Howard Hughes Medical Institute
Harvard Medical School
HHMI/Division of Endocrinology
Children's Hospital Boston
300 Longwood Ave
Boston MA 02115
Phone (617) 919-2846
Fax: (617) 730-0244
Morris.white@childrens.harvard.edu
WHITELAB Product Profile Sheet
About this Form
 This Product Profile Sheet (PPS) is the only form of significant length that Morris asks you to
complete to describe your construct, protein or antibody.
Please respond to all questions if possible. Add comments wherever helpful.
The more you tell me now, the less likely I will need to bother you for additional information. Whenever
possible, please include references, protocols, photos and figures (may be submitted by e-mail or snail
mail).
Tips for completing this form

This MS Word document is constructed of tables.

Set View to Normal under the View Menu.

Use arrow keys or mouse or TAB key to navigate from cell to cell.

Pressing TAB in the final field of a table creates a new row.

Enter responses only in unshaded cells.

Cells will expand to fit content.

Mark multiple choice fields with “X”

Control-Z will undo errors.
To submit: Please email this document as an e-mail attachment to Morris.white@childrens.harvard.edu
Thank you.
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Product Profile Sheet for Recombinant Expression Constructs
(for completion within Microsoft Word and e-mail or fax return)
Note: Type only in non-shaded areas throughout this form.
Date
Contact Information
From the Laboratory of
Lab Contact for Technical Information
Name
Mailing
Address
Phone
Fax Email
Shipping
Address
(if
different)
Descriptive Information
Briefly discuss the structure, nomenclature, activity, cellular function, physiology, etc. of the protein encoded by this cDNA
and types of in vitro assays for which the expressed kinase is suitable.
Publication Information
Are there publications describing the production and/or use of
this cDNA and/or the encoded kinase? If you’d like to use
PubMed, here is the URL: http://www.ncbi.nlm.nih.gov/PubMed/
1
2
3
4
5
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Comments (e.g., applications
illustrated or protocols described)
Will you provide
a reprint or PDF?
Basic Information
Full name of Construct (as designated by your lab)
Expression System
Mammalian
Bacterial
Baculovirus
Yeast
Name of Host Vector
Is this vector available commercially? We will need a map. (please specify vendor and/or URL to product)
cDNA Excision Site(s)
(specify position relative to start codon)
5’
3’
Start codon
encoded within cDNA
provided by vector
Vector Propagation and Protein Expression Information
Vector Propagation Protocol and Notes
(specify reference, or media, antibiotic and concentration, etc.)
Preferred Host Cells for Protein Expression
Protein Expression Protocol and Notes
(specify reference, or media, antibiotic and concentration, etc.)
Protein Purification Protocol and Notes
Calculated Molecular Weight of Expressed Protein
(calculated)
Observed Molecular Weight of Expressed Protein (SDSPAGE)
Approximate Purity of Expressed Protein (%)
Significant Contaminants (if known)
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Approximate Yield of Expressed Protein
Approximate Activity of Expressed Kinase
Comments
Page 4 of 4