Supplementary Information
Protein-based soft micro-optics fabricated by femtosecond laser direct writing
Yun-Lu Sun1, Wen-Fei Dong1,*, Li-Gang Niu1, Tong Jiang1, Dong-Xu Liu1, Lu Zhang1, YingShuai Wang1, Qi-Dai Chen1, Dong-Pyo Kim3, and Hong-Bo Sun1,2,*
Affiliations
1
State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and
Engineering, Jilin University, 2699 Qianjin Street, Changchun, 130012, China
2
College of Physics, Jilin University, 119 Jiefang Road, Changchun, 130023, China
3
National Center of Applied Microfluidic Chemistry, Department of Chemical Engineering,
Pohang University of Science and Technology, Environ. Eng. Bldg., San31, Hyoja-dong,
Nam-gu, Pohang, Gyungbuk, 790-784, Korea
Correspondence:
Prof. Wen-Fei Dong, State Key Laboratory on Integrated Optoelectronics, College of
Electronic Science and Engineering, Jilin University, 2699 Qianjin Street, Changchun,
130012, China
E-mail: wenfeidong@126.com
Prof. Hong-Bo Sun, State Key Laboratory on Integrated Optoelectronics, College of
Electronic Science and Engineering, Jilin University, 2699 Qianjin Street, Changchun,
130012, China
E-mail: hbsun@jlu.edu.cn
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Experimental Details
Optical microscopy characterization: The optical performances, pH treatment and the
flexibility of BSA micro-KPLs were tested by an upright optical microscope (Motic BA400).
The pictures of imaging, focusing and chromatic dispersion were taken by properly tunning
the position of samples along the light axis. White light illumination was from a halogen lamp
and monochromatic incident lights with different wavelengths were obtained by filtering
white light with narrow-band filters (20-nm band width, central wavelengths of 450 nm, 578.4
nm, 492 nm, 618.8 nm, 549 nm and 659 nm, respectively). In the test of pH effect, the protein
micro-KPL was soaked in buffers of different pH values and observed under the optical
microscope without any other change of the setups, including the sample’s position on the
light axis, buffer’s volume, aperture, etc. Thus, the influence of buffers with various pH
values including strong base and acid was visually and faithfully exhibited. Additionally, the
volume of buffer in the sample pool was kept the same every time the buffer was replaced to
maintain a fixed buffer level for excluding liquid level’s influence on the path of light.
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Scheme S1. Schematic of a homemade femtosecond laser 3D direct writing (FsLDW)
approach and the preparation of protein micro-optic. The as formed protein-based micro/nanoelements exhibit excellent morphology, performances, biodegradation and flexibility, etc.
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