Biomineralization mediated by anaerobic
methane-consuming cell consortia
Ying Chen1,2, Yi-Liang Li3, Gen-Tao Zhou4, Han Li4, Yang-Ting Lin5, Xiang Xiao1,2, Feng-Ping Wang1,2*
1
State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology,
Shanghai Jiao Tong University, Shanghai 200240, People’s Republic of China.
State Key Laboratory of Ocean Engineering, School of Naval Architecture, Ocean and Civil
Engineering, Shanghai Jiao Tong University, Shanghai 200240, People’s Republic of China.
2
3
Department of Earth Sciences, The University of Hong Kong, Hong Kong, China.
CAS Key Laboratory of Crust-Mantle Materials and Environments, School of Earth and Space
Sciences, University of Science and Technology of China, Hefei 230026, China
4
Key Laboratory of the Earth’s Deep Interior, Institute of Geology and Geophysics, Chinese
Academy of Sciences, Beijing 100029, China
5
Supplementary Materials：
Supplementary figures and figure legends
Supplementary references
1
Supplementary figure Legends:
Figure S1. XRD (X-ray diffraction) pattern of stored sample, These diffraction peaks can be
indexed to quartz (Q), nacrite (N), calcite (C) and dolomite (D), respectively, compared with the
JCPDS files of No.65-0466, No.76-0631, No.72-1650 and No.74-1687.
2
Figure S2. FTIR (Fourier transform infrared spectroscopy) of stored sample. The vibrational
bands at 1090 cm−1, 799 cm−1, 779 cm−1, 695 cm−1 and 469 cm−1 can be assigned to quartz
characteristic absorption bands
1, 2
. Similarly, the vibrational bands at 3699 cm−1, 3621 cm−1,
3431 cm−1, 1034 cm−1, 914 cm−1, 533 cm−1 and 426 cm−1 are in correspondence with nacrite 3, 4.
The remaining peaks at 1434 cm−1 and 873 cm−1 indicate the existence of calcite or dolomite 5, 6.
3
a
b
1 μm
1 μm
Au
Au
O
O
Mg
Ca
Ca
C
Mg
C
Au
Au
Fe
Ca
c
Au
Au
Ca
d
2 μm
1 μm
Si
O
Al
Au
Si
O
Au
C
Al
C
Mg
Ca
Figure S3. SEM micrographs and EDS microanalyses of stored sample. (a) Coccolith. (b) A
well-defined rhombohedral crystal with an EDS analyzed stoichiometry of dolomite. (c) Nacrite
with platelet morphology. (d) The aggregated material showing a mixture of carbonate,
alluminosilicates. The peaks of Au in all EDS were from coating.
4
a
1 μm
c
1 μm
b
5 1/nm
d
5 1/nm
Figure S4. Selected area electron diffraction analysis on the poorly crystallized and laminated
siliceous materials from stored sample. (a) A monocrystal under TEM; (b) The SAED pattern of
the crystal in (a). (c) the aggregation of siliceous material under TEM; (d) The SAED rings
showed diffraction of multi-crystals. The diffractions of monocrystal and multi-crystal
aggregates consistently gave the structure of kaolinite.
5
Low magnification image covering the whole sample for
localization
High magnification graph
for identification
5 μm
50 μm
Boundary of the
sample drop
Figure S5 Photomicrographs used for cell identification and localization.
6
Supplementary references
1
2
3
4
5
6
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Transform Infrared Spectrophotometer (FTIR): A fast method for identifying Heinrich events in IODP Site
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