Supplementary Information
Biocompatible fluorescent supramolecular nanofibrous hydrogel for longterm cell tracking and tumor imaging applications
Huaimin Wang1*, Duo Mao1*, Youzhi Wang1, Kai Wang1, Xiaoyong Yi2, Deling Kong1,
Zhimou Yang1, Qian Liu2 & Dan Ding1
1
State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive
Materials, Ministry of Education, College of Life Sciences, and Collaborative Innovation
Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin,
300071, P. R. China, 2Department of Urology, Tianjin First Central Hospital, 24 Fukang
Road, Tianjin 300192, P. R. China.
Correspondence
and
requests
for
materials
should
be
addressed
to
D.D.
(dingd@nankai.edu.cn) or Q.L. (simonlq@163.com) or K.W. (wkcs424@163.com).
* These authors contributed equally to this work.
Supplementary Figure S1 1H NMR spectrum of Rhodamine-GFFYE-CS-EE.
mV(x1,000)
CH1 (220nm)
CH2 (254nm)
1.00
0.75
0.50
0.25
0.00
0.0
2.5
5.0
7.5
min
Inten.(x10,000)
828.10
2.5
2.0
1.5
78.30
1.0
1656.70
178.25
0.5
1987.40
370.25
955.95 1104.90
613.05
1397.05 1586.65 1755.20
0.0
250
500
750
1000
1250
1500
1750
Supplementary Figure S2 LC-MS spectrum of Rhodamine-GFFYE-CS-EE.
m/z
Supplementary Figure S3 HPLC spectra of Rhodamine-GFFYE-CS-EE after
treatment with GSH for (A) 1 h, (B) 2 h, (C) 3 h and (D) 17 h.
Normalized intensity (a.u.)
1.0
Absorption
Emission
0.8
0.6
0.4
0.2
0.0
300
400
500
600
700
800
Wavelength (nm)
Supplementary Figure S4 UV-vis absorption and photoluminescence spectra of the
nanofibers in water. Excitation at 553 nm.
B
A
25 μm
C
25 μm
D
25 μm
25 μm
Supplementary Figure S5 CLSM images of the HepG2 cancer cells after incubation
with (A) the nanofiber and (C) precursor for 2 h. (B) and (D) are the corresponding
Normalized intensity (a.u.)
fluorescence/transmission overlay images of (A) and (C), respectively.
Precursor
Nanofiber
1.0
0.8
0.6
0.4
0.2
0.0
560
580
600
620
640
660
Wavelength (nm)
Supplementary Figure S6 Photoluminescence spectra of the precursor and nanofiber
in water. Excitation at 553 nm.
Supplementary Figure S7 CLSM image of the unlabeled HeLa cancer cells.
140
Precursor
Nanofiber
Cell Viability (%)
120
100
80
60
40
20
0
12
24
48
Time (h)
Supplementary Figure S8 Metabolic viability of HeLa cells after incubation with the
precursor and nanofiber at the Rhodamine B concentration of 100 μM for 12, 24 and
48 h.