LAB 17 RESPIRATORY LUNG VOLUMES Objective: To measure and learn the various lung volumes and to understand how they can be used to determine respiratory function. A. Measurement of Individual Lung Volumes Lung volumes vary with age, sex, size and respiratory health. Keep this in mind as measure lung volumes. Obtain a dry spirometer and use it to determine the following: 1. First, measure your vital capacity. Inhale and exhale forcibly several times to warm up. Finally, inhale as much as you can and then exhale as much as you can into the spirometer. Be sure the spirometer is set at zero before you begin each time. Take the reading. Repeat this procedure two more times. a. Calculate your average vital capacity by adding up all three readings and dividing by three. Enter your average vital capacity into your table. Vital Capacity 1 Vital Capacity 2 Vital Capacity 3 TOTAL Avg. Vital Capacity (Total/3) 2. Compare your vital capacity with the expected value for a person your age and size given in the vital capacity table. Enter the expected value into Table 1. 3. Calculate your expected VC below. Show your calculations below and enter the % in Table 1. % Expected VC 4. Your value Expected value 100 Determine your tidal volume (TV) by breathing normally and exhaling into the zeroed spirometer. This should be a normal exhalation, not forced. Repeat this three times, each time after setting the spirometer back to zero. Calculate your average TV and enter it into Table 1 (TV is usually about 10% of your vital capacity). 74 Tidal Volume 1 Tidal Volume 2 Tidal Volume 3 TOTAL Avg. Tidal Volume (Total/3) 5. Determine your expiratory reserve volume (ERV). Do this by exhaling normally (not into the spirometer) then, forcefully exhale as much as you can into the spirometer. Again, be sure the spirometer is set at zero before you begin. Record the reading in Table 1 (ERV is usually about 25% of VC). 6. Calculate your inspiratory reserve volume (IRV) by plugging the known values into the equation below. Show your calculations below and enter the number in Table 1 (IRV is usually about 65% of VC). Vital Capacity = TV + ERV + IRV 7. Calculate your minute respiratory volume. Show your calculations below and enter the value in Table 1. Respiratory rate (breaths/min) Minute Respiratory Volume = TV Respiratory Rate 8. Calculate the dead space. Show your calculations below and enter the number in Table 1. Dead Space = TV x 30% 9. The minute respiratory volume does not take into account the volume of air wasted in the dead space. To get a more accurate measurement of respiratory efficiency, calculate the alveolar ventilation rate (AVR) and enter the number in Table 1. AVR = (TV - Dead Space) Respiratory Rate 10. Residual volume (RV) is an amount of air that remains in the lungs even after a forceful expiration. This air helps to keep the alveoli open and prevent lung collapse. Normally it is around 1200 mL. in males and 1100 mL in females. Keep this in mind as you calculate total lung capacity below and answer the following questions. 75 11. Total lung capacity (TLC) is the sum of all lung volumes (normally around 6000 mL in males). Calculate your TLC and enter the number in Table 1. a. Use your text to write a formula to determine your total lung capacity. Write the formula, show the calculations and your lung capacity as calculated below. TABLE 1 FORMULA Average Vital Capacity Expected Vital Capacity (Table 2) % Expected VC Average Tidal Volume Expiratory Reserve Volume (ERV) Inspiratory Reserve Volume (IRV) Minute Respiratory Volume (MRV) Dead Space Alveolar Ventilation Rate (AVR) Total Lung Capacity (TLC) 76 DATA TABLE 2: Normal Vital Capacity of Adults* (in cubic cm) Age in Years Height (inches) 20 30 40 50 60 70 60 3885 3665 3445 3225 3005 2785 62 4154 3925 3705 3485 3265 3045 64 4410 4190 3970 3750 3530 3310 66 4675 4455 4235 4015 3795 3575 68 4940 4720 4500 4280 4060 3840 70 5206 4986 4766 4546 4326 4106 72 5471 5251 5031 4811 4591 4371 74 5736 5516 5516 5076 4856 4636 58 2989 2809 2629 2449 2269 2089 60 3198 3018 2838 2658 2478 2298 62 3403 3223 3043 2863 2683 2503 64 3612 3432 3252 3072 2892 2710 66 3822 3642 3462 3282 3102 2922 68 4031 3851 3671 3491 3311 3131 70 4270 4090 3910 3730 3550 3370 72 4449 4269 4089 3909 3729 3549 Males Females QUESTIONS 1. What is vital capacity? 2. What is the significance of vital capacity? 3. What is the inspiratory reserve volume? 4. What is the expiratory reserve volume? 5. What is tidal air? 77 6. What volume of air is important in the Heimlich maneuver? 7. What physical principle is demonstrated when performing the Heimlich maneuver? (Review Boyle’s Law) 8. What is the difference between alveolar ventilation and minute respiratory volume? 9. What is dead space? B. OXYGEN-HEMOGLOBIN DISSOCIATION CURVE QUESTIONS 1. What is being measured on the x-axis? _________________________________________ 2. What is being measured on the y-axis? _________________________________________ 3. Based on questions 1 and 2, what does the graph show? 78 4. What is the significance of a PO2 of 100 mm Hg? 5. What is the significance of a PO2 of 40 mm Hg? 6. At a PO2 of 100 mm Hg. what percentage of the total hemoglobin is saturated with O2________________ ? 7. At a PO2 of 40 mm Hg. what percentage of the total hemoglobin is saturated with O 2________________ ? 8. What does the difference between the percentages in questions 6 and 7 represent? 9. What does it mean to shift the curve to the right and what is the result of doing so? 79