Hermann J. Gruber Institute of Biophysics University of Linz A-4040 LINZ, Austria List of Publications 29 October 2007 Patent applications: 1. Gruber, H. J., and Johnson, W. T. (2006) Probe and method of making a probe using crosslinker compositions as chemical and biological sensors. WO/2007/084238. 2. Kienberger, F., Artelsmair, H., Tinazli, A., Rankl, C., Tampé, R., Gruber, H., Hinterdorfer, P., and Blaas, D. (2006) AFM-based nanochip for the detection and characterization of single virus particles. Austrian patent application A 961/2006, patend pending. 3. Gruber, H. J., et al. (2006) Verfahren zur Herstellung von reaktiven Einzelmolekülschicht-Einheiten. Austrian patent application A 1469/2006, patent pending. 4. Zhu, R., Hinterdorfer, P., and Gruber, H. (2007) Method for detecting 5-methylcytosine. WO/2007/087653. Book chapters: 5. Riener, C. K., Kada, G., Borken, C., Kienberger, F., Hinterdorfer, P., Schindler, H., Schütz, G. J., Schmidt, T., Hahn, C. D., and Gruber*, H. J. (2002) Bioconjugation for biospecific detection of single molecules in atomic force microscopy (AFM) and in single dye tracing (SDT). Recent Research Developments in Bioconjugate Chemistry 1, 133-149. 6. Pragl, B., Gruber, H. J., Schütz, G. J., Trieb, M., Prinz, H., and Knaus*, H.-G. (2002) Fluorescently-labeled K+ channel toxins – tools to study K+ channels in native tissues. Recent Research Developments in Bioconjugate Chemistry 1, 83-94. 7. Kienberger,* F., Gruber, H., and Hinterdorfer, P. (2006) Dynamic force microscopy and spectroscopy. Applied scanning probe methods. Volume 2: Scanning probe microscopy techniques (Bushan, B., and Fuchs, H., Eds.) pp 143-164, Chapter 5, Springer-Verlag, Berlin. 8. Kienberger,* F., Chtcheglova, L., Ebner, A., Puntheeranurak, T., Gruber, H., and Hinterdorfer, P. (2007) Single-molecule studies on cells and membranes using the atomic force microscope. Applied scanning probe methods. Volume 6: (Bushan, B., and Fuchs, H., Eds.) pp 101-125, Chapter 14, Springer-Verlag, Berlin. 9. Ebner, A., Marek, M., Kaiser, K., Kada, G., Hahn, C. D., Lackner, B., and Gruber, H. J.* (2007) Application of biotin-4-fluorescein in homogeneous fluorescence assays for avidin, streptavidin and biotin or biotin derivatives. Book chapter invited by Humana Press for the series "Methods in Molecular Biology", accepted for publication. 1 Hermann J. Gruber Institute of Biophysics University of Linz A-4040 LINZ, Austria List of Publications 29 October 2007 Articles in referenced journals: 10. Sterk*, H and Gruber, H. (1984) The relaxational behaviour of self-associated 6methylpurine. J. Am. Chem. Soc. 106, 2239 – 2242. 11. Gruber*, H. J. and Low, P.S. (1988) Interactions of amphiphiles with integral membrane proteins. I. Structural destabilization of the anion transport protein of the erythrocyte membrane by fatty acids, fatty alcohols, and fatty amines, Biochim. Biophys. Acta 944. 414 – 424. 12. Gruber*, H.J. (1988) Interactions of amphiphiles with integral membrane proteins. II. A simple, minimal model for the nonspecific interaction of amphiphiles with the anion exchanger of the erythrocyte membrane. Biochim. Biophys. Acta 944, 425 – 436. Geringer, M., Gruber, H., and Sterk*, H (1991) Self-diffusion and 13C relaxation of self-associated 1,10-phenanthroline. J. Phys. Chem. 95, 2525 – 2529. 13. 14. Gruber*, H. J. and Hansgeorg Schindler (1994) External surface and lamellarity of lipid vesicles: a practice-oriented set of assay methods. Biochim. Biophys. Acta 1189, 212224. 15. Gruber, H.J., Wilmsen, H.U., Cowell, S., Schindler, H., and Buckley*, J.T. (1994) Partial purification of the rat erythrocyte receptor for the channel-forming toxin aerolysin and reconstitution into planar lipid bilayers. Molec. Microbiol. 14, 1093-1101. 16. Seydl, K., Karlsson*, J. O., Dominik, A., Gruber, H. J., and Romanin, C. (1994) Action of calpastatin in prevention of cardiac L-type Ca2+ channel run-down cannot be mimicked by synthetic calpain inhibitors. Pflügers Arch.-Eur. J. Physiol. 429, 503-510. 17. Haselgrübler, T., Amerstorfer, A., Schindler, H., and Gruber*, H. J. (1995) Synthesis and applications of a new poly(ethylene glycol) derivative for the crosslinking of amines with thiols. Bioconjugate Chem. 6, 242-248. 18. Gruber*, H. J., Wilmsen, H. U., Schurga, A., Pilger, A., and Schindler, H. (1995) Measurement of intravesicular volumes by salt entrapment. Biochim. Biophys. Acta 1240, 266-276. 19. Schmidt*, T., Schütz, G. J., Baumgartner, W., Gruber, H. J., and Schindler, H. (1995) Characterization of Photophysics and Mobility of Single Molecules in a Fluid Lipid Membrane. J. Phys. Chem. 99, 17662-17668. 20. Schmidt*, T., Schütz, G.J., Gruber, H. J., and Schindler, H. (1996) Local Stoichiometries determined by counting individual molecules. Anal. Chem. 68, 43974401. 21. Schmidt*, T., Schütz, G. J., Baumgartner, W., Gruber, H. J., and Schindler, H. (1996) Imaging of single molecule diffusion. Proc. Natl. Acad. Sci. USA 93, 2926-2929. 22. Hinterdorfer, P., Baumgartner, W., Gruber, H. J., Schilcher, K., and Schindler*, H. (1996) Detection and localization of individual antibody-antigen recognition events by atomic force microscopy. Proc. Natl. Acad. Sci. USA 93, 3477-3481. 23. Hinterdorfer, P., Gruber, H. J., Striessnigg, J., Glossmann, H., and Schindler*, H. (1997) Analysis of Membrane Protein Selfassociation in Lipid Systems by Fluorescence 2 Hermann J. Gruber Institute of Biophysics University of Linz A-4040 LINZ, Austria List of Publications 29 October 2007 Particle Counting. Application to the Dihydropyridine Receptor. Biochemistry 36, 44974504. 24. Kaiser, K., Marek, M., Haselgrübler, T., Schindler, H., and Gruber*, H. J. (1997) Basic Studies on Heterobifunctional Biotin-PEG Conjugates with a 3-(4Pyridyldithio)propionyl Group on the Second Terminus. Bioconjugate Chem. 8, 545551. 25. Gruber*, H. J., Marek, M., Schindler, H., and Kaiser, K. (1997) Biotin-Fluorophore Conjugates with Poly(ethylene glycol) Spacers Retain Intense Fluorescence after Binding to Avidin and Streptavidin. Bioconjugate Chem. 8, 552-559. 26. Marek, M., Kaiser, K., and Gruber*, H. J. (1997) Biotin-Pyrene Conjugates with Poly(ethylene glycol) Spacers Are Convenient Fluorescent Probes for Avidin and Streptavidin. Bioconjugate Chem. 8, 560-566. 27. Cowell, S., Aschauer, W., Gruber, H. J., Nelson, K. L., and Buckley*, J. T. (1997) The Erythrocyte Receptor for the Channel-Forming Toxin Aerolysin is a Novel Glycosylphosphatidylinositol-Anchored Homologue of the Mammalian ADP-ribosyl Transferases. Molec. Microbiol. 25, 343-350. 28. Schilcher, K., Hinterdorfer, P., Gruber, H. J., and Schindler*, H. (1997) A noninvasive method for the tight anchoring of cells for scanning force microscopy. Cell Biol. Int., 21, 769-778. 29. Schütz, G. J., Gruber, H. J., Schindler, H., and Schmidt*, T. (1997) Fluorophores for single molecule microscopy. J. Luminescence 72-74, 18-21. 30. Hinterdorfer*, P., Schilcher, K., Baumgartner, W., Gruber, H. J., and Schindler, H. (1998) A Mechanistic Study of the Dissoziation of Individual Antibody-Antigen Pairs by Atomic Force Microscopy. Nanobiology 4, 177-188. 31. Altmann, R., Falk*, H., and Gruber, H.J. (1998) Synthesis and Properties of IonophoreConjugated Hypericin Derivatives. Monatshefte für Chemie 129, 235-244. 32. Gruber*, H. J., Kada, G., Marek, M., and Kaiser, K. (1998) Accurate Titration of Biotin-Binding Sites in Avidin and Streptavidin with Biotin-Fluorophore Conjugates. Biochim. Biophys. Acta 1381, 203-212. 33. Trabesinger, W., Schütz, G. J., Gruber, H.J., Schindler, H., and Schmidt*, T. (1998) Detection of Individual Oligonucleotide Pairing by Single Molecule Microscopy. Anal. Chem. 71, 279-283. Willemsen, O. H., Snel, M. M. E., van der Werf, K. O., Gruber, H. J., Hinterdorfer, P., Schindler, H., van Kooyk, Y., de Grooth, B.G., Greve, J., and Figdor*, C.G. (1998) Simultaneous Height and Adhesion of Antibody-Antigen Interactions by Atomic Force Microscopy. Biophys. J. 75, 2220-2228. 35. Obermüller, R. A., Schütz, G. J., Gruber, H. J., and Falk*, H. (1999) Concerning Regioselective Photochemical Intermolecular Proton Transfer from Hypericin. Monatshefte für Chemie 130, 275-281. 3 Hermann J. Gruber Institute of Biophysics University of Linz A-4040 LINZ, Austria List of Publications 29 October 2007 36. Kada, G., Falk, H., and Gruber*, H. J. (1999) Accurate Measurement of Avidin and Streptavidin in Crude Biofluids with a New, Optimized Biotin-Fluorescein Conjugate. Biochim. Biophys. Acta 1427, 33-43. 37. Kada, G., Kaiser, K., Gruber*, H. J. (1999) Rapid Estimation of Avidin and Streptavidin by Fluorescence Quenching or Fluorescence Polarization. Biochim. Biophys. Acta. 1427, 44-48. 38. Harms, G. S., Sonnleitner, M., Schütz, G.J., Gruber, H. J., and Schmidt*, T. (1999) Single Molecule Anisotropy Imaging. Biophys. J. 77, 2864-2870. 39. Gruber*, H. J., Kada, G., Pragl, B., Riener, C., Hahn, C. D., Harms, G. S., Ahrer, W., Dax, T. G., Hohenthanner, K., and Knaus, H.-G. (2000) Preparation of Thiol-reactive Cy5 Derivatives from Commercial Cy5 Succinimidyl Ester. Bioconjugate Chem. 11, 161-166. 40. Schütz*, G. J., Pastushenko, V. P., Gruber, H. J., Knaus, H.-G., Pragl, B., and Schindler, H. (2000) 3D Imaging of Individual Ion Channels in Live Cells at 40 nm Resolution. Single Molecules 1, 25-31. 41. Kienberger, F., Kada, G., Gruber, H. J., Pastushenko, P., Riener, C., Trieb, M., Knaus, H.-G., Schindler, H., and Hinterdorfer*, P. (2000) Recognition Force Spectroscopy Studies of the NTA-His6 Bond. Single Molecules 1, 25-31. 42. Kienberger, F., Pastushenko, V. P., Kada, G., Gruber, H. J., Riener, C., Schindler, H., and Hinterdorfer*, P. (2000) Static and Dynamical Properties of Single Poly(Ethylene Glycol) Molecules Investigated by Force Spectroscopy. Single Molecules 1, 123-128. 43. Hinterdorfer*, P., Kienberger, F., Raab, A., Gruber, H. J., Baumgartner, W., Kada, G., Riener, C., Wielert-Badt, S., Borken, C., and Schindler, H. (2000) Poly(Ethylene Glycol): An Ideal Spacer for Molecular Recognition Force Microscopy/Spectroscopy. Single Molecules 1, 99-103. 44. Baumgartner, W., Gruber, H., Hinterdorfer, P., and Drenckhahn*, D. (2000) Affinity of Trans-Interacting VE-Cadherin Determined by Atomic Force Microscopy. Single Molecules 1, 123-128. 45. Gruber*, H. J., Hahn, C. D., Kada, G., Riener, C, Harms, G. S., Ahrer, W., Dax, T. G., and Knaus, H.-G. (2000) Anomalous Fluorescence Enhancement of Cy3 and Cy3.5 versus Anomalous Fluorescence Loss of Cy5 and Cy7 upon Covalent Linking to Proteins and Noncovalent Binding to Avidin. Bioconjugate Chem. 11, 696-704. 46. Horn*, R., Ding, S., and Gruber, H. J. (2000) Immobilizing the Moving Parts of Voltage-Gated Ion Channels: Implications for Coupling of Voltage Sensors to Gates. J. Gen. Physiol. 116, 461-475. 47. Egwim, I. O. C., and Gruber*, H. J., (2001) Spectrophotometric Measurement of Mercaptans with 4,4'-Dithiodipyridine. Anal. Biochem. 288,188-194. 48. Kada, G., Riener, C. K., and Gruber*, H. J. (2001) Dithio-phospholipids for oriented immobilization of proteins to gold surfaces. Tetrahedron Letters 42, 2677-2680. 4 Hermann J. Gruber Institute of Biophysics University of Linz A-4040 LINZ, Austria List of Publications 29 October 2007 49. Hinterdorfer, P., Gruber, H. J., Kienberger, F., Kada, G., Riener, C., Borken, C., and Schindler, H. (2002) Surface attachment of ligands and receptors for molecular recognition Force microscopy. Coll. Surf. B 23, 115-123. (Colloids and Surfaces B: Biointerfaces) 50. Pragl, B., Koschak, A., Obermair, G., Kaufmann, W., Gerster, U., Blanc, E., Hahn, C. D., Prinz, H., Schütz, G., Darbon, H., Gruber, H. J., and Knaus, H.-G. (2002) Synthesis, characterization, and application of cy-dye- and alexa-dye-labeled hongotoxin(1) analogues. The first high affinity fluorescence probes for voltage-gated K+ channels. Bioconjugate Chem. 13, 416-425. 51. Wielert-Badt, S., Hinterdorfer*, P., Gruber, H. J., Lin, J.-T., Badt, D., Wimmer, B., Schindler, H., and Kinne, R. K.-H. (2002) Single molecule recognition of protein binding epitopes in brush border membranes by force microscopy. Biophys. J. 82, 27672774. 52. Riener, C. K., G. Kada, and Gruber*, H. J. (2002) Quick measurement of protein sulfhydryls with Ellman's reagent and with 4,4'-dithiodipyridine. Anal. Bioanal. Chem. 373, 266-276. 53. Kada, G., Riener, C. K., Hinterdorfer, P., Kienberger, F., Stroh, C. M., and Gruber*, H. J. (2002) Dithio-phospholipids for biospecific immobilization of proteins on gold surfaces. Single Mol. 3, 119-125. 54. Riener, C. K., Stroh, C. M., Ebner, A., Klampfl, C., Gall, A. A., Romanin, C., Lyubchenko, Y. L., Hinterdorfer, P., and Gruber*, H. J. (2003) Simple Test System for Single Molecule Recognition Force Microscopy. Anal. Chim. Acta 479, 59-75. 55. Riener, C. K., Kienberger, F., Hahn, C. D., Buchinger, G. M., Egwim, I. O. C., Haselgrübler, T., Ebner, A., Romanin, C., Klampfl, C., Lackner, B., Prinz, H., Blaas, D., Hinterdorfer, P., and Gruber*, H. J. (2003) Heterobifuctional crosslinkers for linking of single ligand molecules to scanning probes. Anal. Chim. Acta 497, 101-114. 56. Klein, D. C. G., Stroh, C. M., Jensenius, H., van Es, M., Kamruzzahan, A. S. M., Stamouli, A. , Gruber, H. J., Oosterkamp, T. H., and Hinterdorfer, P.* (2003) Covalent Immobilization of Single Proteins on Mica for Molecular Recognition Force Microscopy. ChemPhysChem. 4, 1367-1371. 57. Gamsjaeger, R., Wimmer, B., Kahr, H., Tinazli, A., Picuric, S., Lata, S., Tampe, R., Maulet, Y., Gruber, H. J., Hinterdorfer, P., Romanin, C. (2004) Oriented binding of the His6-tagged carboxyl-tail of the L-type Ca2+ channel 1-subunit to a new NTAfunctionalized self-assembled monolayer. Langmuir 20, 5885-5890. 58. Stroh, C. M., Ebner, A., Geretschläger, M., Freudenthaler, G., Kienberger, F., Kamruzzahan, A. S. M., Smith-Gill, S. J., Gruber, H. J., and Hinterdorfer, P.* (2004) Simultaneous Topography and Recognition Imaging Using Force Microscopy. Biophys. J. 87 (3), 1981-1990. 59. Stroh, C. M., Wang, H., Bash, R., Ashcroft, B., Nelson, J., Gruber, H., Lohr, D., Lindsay, S. M.*, and Hinterdorfer, P. (2004) Single-molecule recognition imaging microscopy. Proc. Natl. Acad. Sci. USA, 101, 12503-12507. 5 Hermann J. Gruber Institute of Biophysics University of Linz A-4040 LINZ, Austria List of Publications 29 October 2007 60. Ebner, A., Kienberger, F., Stroh, C. M., Gruber, H. J., and Hinterdorfer, P.* (2004) Monitoring of Glass Derivatization with Pulsed Force Mode Atomic Force Microscopy. Micros. Res. Tech 65, 246-251. 61. Ebner, A., Kienberger, F., Kada, G., Stroh, C. M., Geretschlager, M., Kamruzzahan, A. S. M., Wildling, L., Johnson, W. T., Ashcroft, B., Nelson, J., Lindsay, S. M., Gruber, H. J., and Hinterdorfer, P.* (2005) Localization of single avidin-biotin interactions using simultaneous topography and molecular recognition imaging. ChemPhysChem. 6, 897900. 62. Kamruzzahan, A. S. M., Kienberger, F., Stroh, C. M., Berg, J., Huss, R., Ebner, A., Zhu, R., Rankl, C., Gruber, H. J., and Hinterdorfer, P* (2004) Imaging morphological details and pathological differences of red blood cells using tapping mode AFM. Biol. Chem. 385, 955-960. 63. Füreder-Kitzmüller, E., Hesse, J., Ebner, A., Gruber, H. J., and Schütz, G. (2005) Nonexponential bleaching of single bioconjugated Cy5 molecules. Chem. Phys. Lett., 404, 13-18. 64. Bonanni, B., Kamruzzahan, A. S. M., Bizzarri, A. R., Rankl, C., Gruber, H. J., Hinterdorfer, P., and Cannistraro, S.* (2005) Single molecule recognition between cytochrome C 551 and gold-immobilized azurin by force spectroscopy. Biophys.l J. 89, 2783-2791. 65. Gamsjäger, R., Johs, A., Gries, A., Gruber, H. J., Romanin, C., Prassl, R., and Hinterdorfer, P.* (2005) Membrane binding of beta-2 glycoprotein I can be described by a two-state reaction model – an atomic force microscopy and surface plasmon resonance study. Biochem. J. 389, 665-673. 66. Kienberger, F., Ebner, A., Gruber, H. J., and Hinterdorfer, P.* (2006) Molecular Recognition Imaging and Force Spectroscopy of Single Biomolecules. Accounts Chem. Res. 39, 29-36. 67. Ebner, A., Kienberger, F., Huber, C., Kamruzzahan, A. S., Pastushenko, V. P., Tang, J., Kada, G., Gruber, H. J., Sleytr, U. B., Sara, M., and Hinterdorfer, P.* (2006) Atomicforce-microscopy imaging and molecular-recognition-force microscopy of recrystallized heterotetramers comprising an S-layer-streptavidin fusion protein. ChemBioChem. 7, 588-591. 68. Puntheeranurak, T., Wildling, L., Gruber, H. J., Kinne, R. K., and Hinterdorfer, P.* (2006) Ligands on the string: single-molecule AFM studies on the interaction of antibodies and substrates with the Na+-glucose co-transporter SGLT1 in living cells. J. Cell. Sci. 114, 2960-2967. 69. Chtcheglova L. A., Vogel, M., Gruber, H. J., Dietler, G., and Haeberli, A. (2006) Kinetics of the interaction of desAABB–fibrin monomer with immobilized fibrinogen. Biopolymers 63, 69-82. 70. Kamruzzahan, A. S. M., Ebner, A., Wildling, L., Kienberger, F., Riener, C. K., Hahn, C. D., Pollheimer, P. D., Winklehner, P., Hölzl, M., Lackner, B., Schörkl, D. M., 6 Hermann J. Gruber Institute of Biophysics University of Linz A-4040 LINZ, Austria List of Publications 29 October 2007 Hinterdorfer, P., and Gruber, H. J. (2006) Antibody linking to atomic force microscope tips via disulfide bond formation. Bioconjugate Chem. 17, 1473-1481. 71. Hahn, C. D., Tinazli, A., Hölzl, M., Leitner, C., Frederix, F., Lackner, B., Müller, N., Klampfl, C., Tampé, R., and Gruber, H. J.* (2007) Pragmatic studies on proteinresistant self-assembled monolayers. Chemical Monthly, 138, 245-252. 72. Hahn, C. D., Leitner, C., Weinbrenner, T., Schlapak, R., Tinazli, A., Tampé, R., Lackner, B., Steindl, C., Hinterdorfer, P., Gruber, H. J., and Hölzl, M.* (2007) Selfassembled monolayers with latent aldehydes for protein immobilization. Bioconjugate Chem. 18, 247-253. 73. Ebner, A., Madl, J., Kienberger, F., Chtcheglova, L. A., Puntheeranurak, T., Zhu, R., Tang, J., Stroh, C., Gruber, H. J., Schütz, G. J., and Hinterdorfer, P.* (2007) Single molecule force microscopy on cells and biological membranes. Curr. Nanosci. 3, 49-56. 74. Puntheeranurak, T., Wimmer, B., Castaneda, F., Gruber, H. J., Hinterdorfer, P., Kinne, R. K. H.* (2007) Substrate Specificity of Sugar Transport by Rabbit SGLT1: Single molecule AFM versus Transport Studies. Biochemistry, 47, 2797-2804. 75. Ebner,* A., Hinterdorfer, P., and Gruber, H. J. (2007) Comparison of different aminofunctionalization strategies for attachment of single antibodies to AFM cantilevers. Ultramicroscopy, in press. 76. Gilbert, Y., Deghorain, M., Wang, L., Xu, B., Pollheimer, P. D., Gruber, H. J., Errington, J., Hallet, B., Haulot, X., Verbelen, C., Hols, P., Dufrêne, Y. F.* (2007) Single-molecule force spectroscopy and imaging of the vancomycin/D-Ala-D-Ala interaction. NanoLetters 7, 796-801. 77. Hölzl, M. Tinazli, T., Leitner, C., Hahn, C. D., Lackner, B., Tampé, R., and Gruber*, H. J. (2007) Protein-resistant self-assembled monolayers on gold with latent aldehyde functions. Langmuir, 23, 5571-5577. 78. Ebner, A., Wildling, L.. Kamruzzahan, A. S. M., Rankl, C., Wruss, J., Hahn, C. D., Hölzl, M., Kienberger, F., Blaas, D., Hinterdorfer, P., and Gruber, H. J.* (2007) A new, simple method for linking of antibodies to atomic force microscopy tips. Bioconjugate Chem. 18, 1176-1184. 79. Schlapak, R., Armitage, D., Saucedo-Zeni, N., Latini, G., Gruber, H. J., Samotskaya , G., Mesquida, P., Cacialli, F., Hohage, M., and Howorka, S.* (2007) Preparation and Characterization of Dense Films of Poly(amidoamine) Dendrimers on Indium Tin Oxide. Langmuir 23, 89169-8924. 80. Waschke, J., Menendez-Castro, C., Bruggeman, P., Koob, R., Amagai, M.; Gruber, H. J., Drenckhahn, D., and Baumgartner, W.* (2007) Imaging and Force Spectroscopy on Desmoglein 1 Using Atomic Force Microscopy Reveal Multivalent Ca2+-Dependent, Low-Affinity Trans-Interaction. J. Membrane Biol. 216, 83-92. 7 Hermann J. Gruber Institute of Biophysics University of Linz A-4040 LINZ, Austria List of Publications 29 October 2007 81. Tang, J., Krajcikova, D., Zhu, R., Ebner, A., Gruber, H. J., Barak, I., and Hinterdorfer, P.* (2007) Atomic Force Microscopy Imaging and Molecular Recognition Force Spectroscopy of Coat Proteins on the Surface of Bacillus subtilis Spore. J. Mol. Rec., in press. 82. Verbelen, C., Gruber, H. J. and Dufrêne, Y. F. (2007) The NTA-His6 bond is strong enough for AFM single-molecular recognition studies. J. Mol. Rec., in press. 83. Kienberger, F., Ebner, A., Chtcheglova, L., Wildling, L., Puntheeranurak, T., Gruber, H. J., and Hinterdorfer, P. (2007) AFM-Tip Chemistry for Single Molecule Recognition Imaging. NSTI Nanotech. 2, 557-560. 84. Rankl, F. Kienberger, H. Gruber, D. Blaas, P. Hinterdorfer. (2007) Accuracy estimation in force spectroscopy experiments. Jpn. J. Appl. Phys. 46, 5536-5539. 85. Ebner, A., Wildling, L., Zhu, R., Haselgrüber, Th., Hinterdorfer, P., and Gruber, H. J.* (2007) Functionalization of Probe Tips and Supports for Single Molecule Recognition Force Microscopy. Top. Curr. Chem., invited review, in press. 86. Puntheeranurak T., Kinne R.K.H., Gruber H.J., and Hinterdorfer P. (2007) SingleMolecule AFM Studies of Substrate Transportation of Sodium-Glucose Cotransporter SGLT1. Journal of the Korean Physical Society, in press. Submitted: 87. Artelsmair, H., Kienberger,* F., Tinazli, A., Schlapak, R., Preiner, J., Kada, G., Wruss, J., Kastner, M., Zhu, R., Hoelzl, M., Rankl, C., Howorka, S., Blaas, D., Gruber, H. J., Tampé, R., and Hinterdorfer, P. (2007) Atomic force microscopy-based nano-chip for the detection of human rhinovirus. Submitted to Small. 88. Tang, J., Ebner, A., Huber, C., Ilk, N., Zhu, R., Pum, D., Leitner, M., Pastushenko, V., Gruber, H. J., Sleytr, U. B., and Hinterdorfer, P. (2007) High Affinity Tags Fused in SLayer Probed by Atomic Force Microscopy. Invited by, and submitted to Langmuir. 89. Graz, I., Ebner, A., Bauer, S., Romanin, C., and Gruber, H: J. (2007) Micropatterned atmospheric pressure discharge surface modification of fluorinated polymer films for mammalian cell adhesion and protein binding. Submitted manuscript. 90. Graz, I., Ebner, A., Bauer, S., Romanin, C., and Gruber, H. J. (2007) Atmospheric pressure discharge surface modification of fluorinated polymer films. Submitted manuscript. In non-referenced journals: 8 Hermann J. Gruber Institute of Biophysics University of Linz A-4040 LINZ, Austria List of Publications 29 October 2007 Ebner, A., Gruber, H. J., und Hinterdorfer, P. (2006) Molekulare Kraftmikroskopie und Kraftspektroskopie. Biospektrum 12, 501-503. Preiner, J., Artelsmair, H., Ebner, A., Kienberger, F., Gruber, H. J., und Hinterdorfer, P. (2006) Charakterisierung von einzelnen Molekülen: Analytik im Nanoraum. GIT Laborfachzeitschrift. Kienberger, F., Ebner, A., Stroh, C., Gruber, H., and Hinterdorfer, P. (2004) Fishing out individual proteins with AFM. BIOforum Europe 06, 66-68. 9