Things You Need to Know About Tissue Culture
This lab practices biosafety level 2 containment.
Lab personnel should be aware of the biohazards they are working with.
This lab uses Human and Murine cell lines as well as Retro/Lenti viruses. These agents
have the potential of infecting humans and may harbor unknown viruses. The following
agents are either in use or have been used in this lab:
Cell Lines & Viruses Kept in This Lab
Murine
Human
129 feeders
COS
NIH/3T3 embryo fibroblasts
MEL erythro leukemia
Hu 11 erythroleukemial cells/human chrom. 11
F9 embryonic carcinoma
Rat II rat fibroblasts
J1 ES cells
7ab ES cells dnmt a/b null
OP-9
HDAC
RB
K562 Chronic myelogenous leukemia
PA-1 embryonic carcinoma
HeLa epitheloid carcinoma, cervix
Jurkat T cell line
EBC-1 squamous cell lung carcinoma (Japan)
LK-1 squamous cell lung carcinoma (Japan)
KMOE erythroid cells
HEL erythroleukemia
CV-1 kidney epithelial cell line
Retrovirus Producers
Assorted retrovirus vectors
PG13 amphotropic packaging cells
PA317 amphotropic packaging cells
Phoenix amphotropic packaging cells
Phoenix ecotropic packaging cells
Lentivirus Producers
HeK 293
MSCV neo vectors and eGFP vectors
MND
HSC
PNP MSCV with PNP inserts
Lentivectors
RRL/CCL (pgkeGFP) & HPV-436 derived
EF1- alpha lentiviral vectors
Virus stocks- Ebv Epstein Bar Virus infected B cell line, HSC, MSCV, Lenti, beta gamma globin
hybrids. ( Most viruses contain eGFP as a reporter)