Standard Operating Procedures
Laboratory Specific
Chemical: Duboisine aka hyoscyamine
IUPAC name (8-methyl-8-azabicyclo[3.2.1]oct-3-yl) 3-hydroxy-2-phenylpropanoate
Please fill out the form completely. Print a copy and insert into your
Laboratory Safety Manual and Chemical Hygiene Plan.
Refer to instructions for assistance.
_____________________________________________________________________________
Department:_____________________
Date when SOP was written:_____________
Date when SOP was approved by the lab supervisor: ___________________
Principal Investigator:___________________________________________________
Internal Laboratory Safety Coordinator/Lab Manager:___________________________________
Laboratory Phone:____________________ Office Phone:_____________________
Emergency Contact:____________________________________________________
(Name and Phone Number)
Location(s) covered by this SOP:__________________________________________
(Building/Room Number)
_____________________________________________________________________________
Type of SOP:
Process
Hazardous Chemical
Hazardous Class
Purpose
An alkaloid obtained from the leaves of an Australian tree (Duboisia myoporoides), and regarded
as identical with hyoscyamine. It produces dilation of the pupil of the eyes.
Synonyms: hyoscyamine, L-Hyoscyamine, Daturine, Duboisine, Hyoscyamine (L), L-Tropine
tropate, 2arm, (-)-Hyoscyamine, (S)-Atropine, Prestwick_273, Hyoscyamine (USP), (-)-Atropine,
ATROPINE, Tropine, (-)-tropate, (S)-(-)-Hyoscyamine.
Apoisonous alkaloid, hyoscyamine , obtained from henbane and other solanaceous plants, is
used as a sedative, analgesic, mydriatic, and antispasmodic.
1
Physical & Chemical Properties/Definition of Chemical Group
CAS#:
101-31-5
Class:
Highly Toxic!!!
Molecular formula:
C17H23NO3
Boiling Point:
N/A
Melting Point:
108.5 deg C
Potential Hazards/Toxicity
EMERGENCY OVERVIEW: Appearance: white powder. May be combustible at high
temperature. Hazardous Decomposition Products: Carbon monoxide, Carbon dioxide.
Danger! Toxic by ingestion, inhalation, or when in contact with skin. Severe over-exposure can
result in death.
Target Organs: Central Nervous system. Peripheral nervous system.
Potential Health Effects:
Eye: There is some evidence to suggest that this material can cause eye irritation and damage in
some persons. When in contact with the eyes, one can experience stinging, dryness, redness,
itch, dilated pupils, and loss of focus with blurred vision. Pupil reflexes may be lost or diminished
for 3 days.
Skin: Skin contact with the material may produce severely toxic effects; systemic effects may
result following absorption and these may be fatal. The material is not thought to be a skin irritant
(as classified using animal models). Abrasive damage however, may result from prolonged
exposures. Open cuts, abraded or irritated skin should not be exposed to this material. Entry into
the blood-stream, through, for example, cuts, abrasions or lesions, may produce systemic injury
with harmful effects.
Ingestion: Severely toxic effects may result from the accidental ingestion of the material. Effects
associated with their use include increased heart rate, decreased saliva production and
other secretions and reduction in bowel movements.
2
Inhalation: Highly toxic by inhalation. Inhalation of dusts, or fume, especially for prolonged
periods, may produce respiratory discomfort and occasionally, distress. Persons with impaired
respiratory function, airway diseases and conditions such as emphysema or chronic bronchitis,
may incur further disability if excessive concentrations of particulate are inhaled.
Chronic: Severe over-exposure can result in death.
Personal Protective Equipment (PPE)
Eyes: Splash goggles designed to minimize the possibility of liquid of vapor eye contact.
Skin: Gloves are required to prevent skin exposure.
Clothing: Where possibility of gross splashing exists, full protective clothing made of rubber,
neoprene or vinyl-coated materials should be worn. Otherwise, wear long pants, closed toed
shoes and a lab coat.
Respirators: Must wear a respirator to prevent dust inhalation. Follow the OSHA respirator
regulations found in 29 CFR 1910.134 or European Standard EN 149. Always use a NIOSH or
European Standard EN 149 approved respirator when necessary.
Engineering Controls
Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety
shower. Use only in a chemical fume hood. If user operations generate dust, fume or mist, use
ventilation to keep exposure to airborne contaminants below the exposure limit. Make sure to
wear splash goggles, a lab coat, gloves and a dust respirator.
First Aid Procedures
Eyes: In case of contact, immediately flush eyes with plenty of water for at least 15 minutes. Do
not use an ointment. Seek medical attention.
Skin: In case of contact, immediately flush skin with plenty of water for at least 15 minutes while
removing contaminated clothing and shoes. Get medical aid immediately. Wash clothing before
reuse.
Ingestion: Call the poison control center at 1-800-222-1222. If swallowed, do not induce
vomiting unless directed to do so by medical personnel. Never give anything by mouth to an
unconscious person. Get medical aid.
Inhalation: POISON chemical! If inhaled, get medical aid immediately. Remove victim to
fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen.
Notes to Physician: Treat symptomatically and supportively.
Special Handling and Storage Requirements
3
Handling: Wear protective splash googles, respirator, gloves and lab attire at all times while
handling this chemical. Use only in a fume hood. Ground and bond containers when transferring
material. Do not get in eyes, on skin, or on clothing. Empty containers retain product residue,
(liquid and/or vapor), and can be dangerous. Keep away from sources of ignition. Empty
containers pose a fire risk, evaporate the residue under a fume hood. Do not ingest or inhale.
Keep away from heat.
Storage: Keep container dry. Keep in a cool place. Ground all equipment containing material.
Keep container tightly closed. Keep in a cool, well-ventilated place. Highly toxic or infectious
materials should be stored in as separate secondary container.
Spill and Accident Procedure
Chemical Spill Dial 911 and x59797
Spill – Vacuum or sweep up material and place into a suitable disposal container. Reduce
airborne dust and prevent scattering by moistening with water. Clean up spills immediately,
observing precautions in the Protective Equipment section. Avoid generating dusty conditions.
Remove all sources of ignition. Use a spark-proof tool. If the material is dry, explosives experts
may be necessary to dispose of the spill. Provide ventilation.
Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate
personal protective equipment and clean-up material for chemical spilled. Double bag spill waste
in clear plastic bags, label and take to the next chemical waste pick-up.
Large (>1 L) – Dial 911 (or 310-825-1491 from cell phone) and EH&S at x59797 for
assistance.
Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in
emergency shower for at least 15 minutes. Seek medical attention. Notify supervisor and EH&S
at x59797 immediately.
Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with
water for 15 minutes by forcibly holding the eye open. Seek medical attention. Notify supervisor
and EH&S at x59797 immediately.
Medical Emergency Dial 911 or x52111
Life Threatening Emergency, After Hours, Weekends And Holidays – Dial 911
(or 310-825-1491 from cell phone) or contact the Ronald Reagan UCLA Medical Center
(emergency room) directly at x52111 (located at 757 Westwood Plaza, enter from Gayley
Avenue). Note: All serious injuries must be reported to EH&S at x59797 within 8 hours.
Non-Life Threatening Emergency– Go to the Occupational Health Facility (OHF),
x56771, CHS room 67-120 (This is on the 6th floor, 7th corridor, room 120. Enter through the
School of Dentistry on Tiverton Drive and proceed to the “O” elevator to the 6th floor.)Hours: M F, 7:30 a.m. to 4:30 p.m. At all other times report to Ronald Regan UCLA Medical Center
(emergency room) at x52111. Note: All serious injuries must be reported to EH&S at x59797
within 8 hours.
4
Needle stick/puncture exposure (as applicable to chemical handling procedure)– Wash
the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane
exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick
nurse by dialing 231 from a campus phone, enter 93333 when prompted and then enter your
extension. Hours: M – F, 8:00 a.m. to 4:00 p.m. At all other times report to Ronald Regan UCLA
Medical Center (emergency room) at x52111. Note: All needle stick/puncture exposures must be
reported to EH&S at x59797 within 8 hours.
Decontamination/Waste Disposal Procedure
Waste disposal procedures
Clean up spills immediately, observing precautions in the Protective Equipment section. Avoid
raising dust and inhalation. Provide ventilation. Ventilate area and wash spill site after material
pickup is complete.
Material Safety Data Sheet (MSDS) Location
(State the location of MSDS)
Hardcopy or electronic copy must be available.
Online MSDS can be accessed at http://msds.ehs.ucla.edu.
Protocol/Procedure
(Add specific description of procedure.)
Note: Any deviation from this SOP requires written approval from PI.
Documentation of Training (signature of all users is required)
I have read and understand the content of this SOP:
Name
Signature
5
Date