Standard Operating Procedures
Laboratory Specific
Chemical: URACIL MUSTARD
aka 2,4 (1H,2H)-Pyrimidinedione or Uramustine
Please fill out the form completely. Print a copy and insert into your
Laboratory Safety Manual and Chemical Hygiene Plan.
Refer to instructions for assistance.
_____________________________________________________________________________
Department:_____________________
Date when SOP was written:_____________
Date when SOP was approved by the lab supervisor: ___________________
Principal Investigator:___________________________________________________
Internal Laboratory Safety Coordinator/Lab Manager:___________________________________
Laboratory Phone:____________________ Office Phone:_____________________
Emergency Contact:____________________________________________________
(Name and Phone Number)
Location(s) covered by this SOP:__________________________________________
(Building/Room Number)
_____________________________________________________________________________
Type of SOP:
Process
Hazardous Chemical
Hazardous Class
Purpose
Uracil Mustard is a chemotherapy drug which belongs to the class of alkylating agents. It is used
for its antineoplastic properties. It is used in lymphatic malignancies such as non-Hodgkin's
lymphoma. It works by damaging DNA, primarily in cancer cells that preferentially take up the
uracil due to their need to make nucleic acids during their rapid cycles of cell division. At high
concentrations of the drug, cellular RNA and protein synthesis are also suppressed. The DNA
damage leads to apoptosis of the affected cells. Chemically it is a derivative of nitrogen mustard
and uracil.
1
Physical & Chemical Properties/Definition of Chemical Group
CAS#:
66-75-1
Class:
Carcinogen, Highly Toxic by ingestion, Irritant!!
Molecular formula:
C8H11Cl2N3O2
Boiling Point:
N/A
Melting Point:
206.1 ° C
Potential Hazards/Toxicity
EMERGENCY OVERVIEW:
Danger! The major hazards encountered in the use and handling of uracil mustard stem from its
toxicological properties. Exposure to this odorless, off-white, crystalline powder may occur from
its manufacture, formulation, or distribution for use as an antineoplastic drug. Effects from
exposure may include nausea, vomiting, diarrhea, dermatitis, irritability, depression, leukopenia,
thrombocytopenia, and anemia. Uracil mustard has been indicated as possibly carcinogenic to
humans. Hazardous decomposition products formed under fire conditions. - Carbon oxides,
nitrogen oxides (NOx), Hydrogen chloride gas.
Potential Health Effects:
Eye: There is some evidence to suggest that this material can cause eye irritation and damage in
some persons.
Skin: Skin contact with the material may initially produce irritation. Systemic effects may result
following absorption and these may be fatal. Avoid contact with skin.
Ingestion: Severely toxic effects including fatality may result if ingested. Effects associated with
their use include increased heart rate, decreased saliva production and other secretions and
reduction in bowel movements. Ingestion LD50 for rats is 3.55mg/kg.
Inhalation: Highly toxic by inhalation. Avoid dust formation. Inhalation of dusts, or fume,
especially for prolonged periods, may produce respiratory discomfort and occasionally, distress.
Persons with impaired respiratory function, airway diseases and conditions such as emphysema
or chronic bronchitis, may incur further disability if excessive concentrations of particulate are
inhaled. Hazardous decomposition products formed under fire conditions are Carbon oxides,
nitrogen oxides (NOx), Hydrogen chloride gas.
2
Personal Protective Equipment (PPE)
Eyes: Safety goggles should be worn to minimize the possibility of liquid of vapor or dust eye
contact.
Skin: Handle with gloves. Gloves must be inspected prior to use. Use proper glove removal
technique (without touching glove's outer surface) to avoid skin contact with this product. Dispose
of contaminated gloves after use in accordance with applicable laws and good laboratory
practices. Wash and dry hands.
Clothing: Employees should be provided with and required to use impervious clothing, gloves,
and safety glasses. Employees should wear long pants, closed toe shoes and a lab coat at all
times.
Respirators: Wear respiratory protection is required. Avoid breathing vapors, mist or gas.
Ensure adequate ventilation. Where risk assessment shows air-purifying respirators are
appropriate use a dust mask type N95 (US) or type P1 (EN 143) respirator. Where risk
assessment shows air-purifying respirators are appropriate use a full-face particle respirator type
N99 (US) or type P2 (EN 143) respirator cartridges as a backup to engineering controls. If the
respirator is the sole means of protection, use a full-face supplied air respirator. Use respirators
and components tested and approved under appropriate government standards such as NIOSH
(US) or CEN (EU).
Engineering Controls
Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety
shower. Use only in a chemical fume hood. Make sure to wear splash goggles, a lab coat, gloves
and the appropriate respirator. Avoid contact with skin and eyes. Avoid formation of dust and
aerosols. Provide appropriate exhaust ventilation at places where dust is formed. Normal
measures for preventive fire protection.
First Aid Procedures
Eyes: In case of contact, immediately flush eyes with plenty of water for at least 15 minutes. Do
not use an ointment. Seek medical attention.
Skin: In case of contact, immediately flush skin with plenty of water for at least 15 minutes while
removing contaminated clothing and shoes. Get medical aid immediately. Wash clothing before
reuse.
Ingestion: Call the poison control center at 1-800-222-1222. If swallowed, do not induce
vomiting unless directed to do so by medical personnel. Never give anything by mouth to an
unconscious person. Get medical aid.
Inhalation: POISON chemical! If inhaled, get medical aid immediately. Remove victim to
fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen.
Notes to Physician: Treat symptomatically and supportively.
3
Special Handling and Storage Requirements
Handling: Wear protective splash goggles, respirator, gloves and lab attire at all times while
handling this chemical. Use only in a fume hood. This chemical is known to be carcinogenic. It is
stable under recommended storage conditions.
Storage: Uracil mustard should be stored and transported in air-tight glass bottles or ampoules
which are, in turn, placed inside strong screw-cap or snap-top containers. Empty drums should be
decontaminated; never re-use containers. Keep in a well-ventilated room.
Spill and Accident Procedure
Chemical Spill Dial 911 and x59797
Spill – Spills of uracil mustard should be taken up with a wet mop or vacuum cleaner equipped
with a high efficiency particulate filter on the exhaust. Do not sweep, brush, or use any other
method which may disperse the substance. Clean up spills immediately, observing precautions in
the Protective Equipment section. Avoid generating dusty conditions. Remove all sources of
ignition. Use a spark-proof tool. If the material is dry, explosives experts may be necessary to
dispose of the spill. Provide ventilation.
Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate
personal protective equipment and clean-up material for chemical spilled. Double bag spill waste
in clear plastic bags, label and take to the next chemical waste pick-up.
Large (>1 L) – Dial 911 (or 310-825-1491 from cell phone) and EH&S at x59797 for
assistance.
Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in
emergency shower for at least 15 minutes. Seek medical attention. Notify supervisor and EH&S
at x59797 immediately.
Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with
water for 15 minutes by forcibly holding the eye open. Seek medical attention. Notify supervisor
and EH&S at x59797 immediately.
Medical Emergency Dial 911 or x52111
Life Threatening Emergency, After Hours, Weekends And Holidays – Dial 911
(or 310-825-1491 from cell phone) or contact the Ronald Reagan UCLA Medical Center
(emergency room) directly at x52111 (located at 757 Westwood Plaza, enter from Gayley
Avenue). Note: All serious injuries must be reported to EH&S at x59797 within 8 hours.
Non-Life Threatening Emergency– Go to the Occupational Health Facility (OHF),
x56771, CHS room 67-120 (This is on the 6th floor, 7th corridor, room 120. Enter through the
School of Dentistry on Tiverton Drive and proceed to the “O” elevator to the 6th floor.)Hours: M F, 7:30 a.m. to 4:30 p.m. At all other times report to Ronald Regan UCLA Medical Center
(emergency room) at x52111. Note: All serious injuries must be reported to EH&S at x59797
within 8 hours.
4
Needle stick/puncture exposure (as applicable to chemical handling procedure)– Wash
the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane
exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick
nurse by dialing 231 from a campus phone, enter 93333 when prompted and then enter your
extension. Hours: M – F, 8:00 a.m. to 4:00 p.m. At all other times report to Ronald Regan UCLA
Medical Center (emergency room) at x52111. Note: All needle stick/puncture exposures must be
reported to EH&S at x59797 within 8 hours.
Decontamination/Waste Disposal Procedure
Waste disposal procedures
Dispose of waste as Hazardous Waste in the appropriate manner as recommended by the
manufacturer.
Material Safety Data Sheet (MSDS) Location
(State the location of MSDS)
Hardcopy or electronic copy must be available.
Online MSDS can be accessed at http://msds.ehs.ucla.edu.
Protocol/Procedure
(Add specific description of procedure.)
Note: Any deviation from this SOP requires written approval from PI.
Documentation of Training (signature of all users is required)
I have read and understand the content of this SOP:
Name
Signature
Date
____________________
___________________
___________________
____________________
___________________
___________________
____________________
___________________
___________________
____________________
___________________
___________________
____________________
___________________
___________________
____________________
___________________
___________________
5
____________________
___________________
___________________
____________________
___________________
___________________
____________________
___________________
___________________
____________________
___________________
___________________
____________________
___________________
___________________
____________________
___________________
___________________
____________________
___________________
___________________
____________________
___________________
___________________
____________________
___________________
___________________
____________________
___________________
___________________
6