University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Hygiene and Ecology, Biology
and Wildlife Diseases
Subject: Biology, 1st year (FVL, FVHE, master study programme)
SYLABUS OF PRACTICALS – WINTER SEMESTRE (2015/2016)
Week Contents
INTRODUCTION, MICROSCOPIC TECHNIQUES
1.
 organizations of practice, protocols, light microscopes, principles of microscopy, dry lenses,
focusing of an object in the visual field, opticle planes, centering the object
EUKARYOTES – ANIMAL CELL AND PROTOZOA
2.
 shape of cells, organelles, shape and number of nuclei
 individual work – monohybridism and dihybridism
MOVEMENT AND IRRITATION, NATIVE SAMPLES
 Brownian molecular motion, amoeboid, ciliar and flagellar movement, cells with cilia,
3.
striated muscle, oxygenotaxis of protozoa
4.
5.
6.
7.
8.
CHEMICAL COMPOSITION OF CELL AND PROKARYOTES
 smears and detection of fat, protein and starch, smears of bacteria, staining, observation under
immersion, measurement
 individual work – sex and inheritance, genetic linkage
BLOOD EXAMINATION
 blood smear and staining, avian and mammalian erythrocytes, measurement
 osmotic phenomena - haemolysis, plazmorhysis, phagocytosis, blood group testing
CELL CYCLE, MITOSIS
 plant cell - onion root, animal cell - tissue culture, intestine, uterus, testis
 consultation – genetic examples
EUKARYOTES – PLANT CELLS
 cell wall, chloroplasts, vacuoles, anthocyanins, osmotic phenomena - plasmolysis, turgor
 first credit test
 individual work – gene interaction, nonmendelian inheritance
REPRODUCTION AND DEVELOPMENT
 meiosis, spermatogenesis, oogenesis, oestral cycle, development of individuals
genetic experiment with Drosophila melanogaster – the beginning of experiment
CYTOGENETIC, MOLECULAR BIOLOGY I
9.
 polytenne chromosome, meiosis, karyotypes, genetic experiment with Drosophila
melanogaster - the removal of adult
 preparation of tissue for DNA isolation
 individual work – population and quantitative genetics
MOLECULAR BIOLOGY II
10.
 genetic experiment with Drosophila melanogaster – evaluation
 DNA isolation, PCR preparation, PCR
MOLECULAR BIOLOGY III
11.
 restriction of PCR product, DNA electrophoresis, visualization and evaluation of PCR
products, demonstration of DNA sequencing
 consultation – genetic examples
CELL CULTURES, GENETICS
12.
13.
 cell culture, working with an inverse microscope
 experiments on animals
 second credit test
CREDITS
Garant: doc. MVDr. Eva Bártová, Ph.D.
On the webpage of department you will find study materials: lectures, sylabus, questions for examination,
and interactive tools (tasks for practicals, foto, video, tests) http://fvhe.vfu.cz/informace-o-fakulte/sekceustavy/ubchvzz/materialy/biology-english-master.html