Molecular Biology – Schedule of Seminars for 2nd year 3rd semester academic year
2014/2015, winter semester. Seminar leader Jacek Anioł, Department of General, Molecular
Biology and Genetics
Medical University of Silesia.
Date
October 07,
2014
October 28,
2014
Topic
1 Structure of the genetic material analysis:
a) bacteria
b) viruses
c) eukaryotic cells
d) hnRNA, folding of the exons, introns fate
e) structure of mRNA, cDNA
2 Molecular biology tools:
a) methods of DNA amplification (inside and outside the cell
replication)
b) variants of PCR
c) methods of RNA amplification, reverse transcription
d) DNA restriction and ligation enzymes, enzymatic
manipulations
e) the use of molecular biology tools in medical diagnostics
November 04, 3 Methods of DNA and RNA amplification outside the
2014
cell - the basics
a) receiving of genetic material, colony PCR, percentage of
cell's RNA
b) methods of qualitative and quantitative analysis of genetic
material and storage conditions
c) principles of oligonucleotide PCR primers design,
oligodT
d) PCR reaction conditions, the effect of various parameters
e) methods of PCR product analysis, Real Time PCR
November 18, 4 Selected methods of detailed analysis of the genetic
2014
material
a) RFLP, DNA fingerprinting
b) Hybridization of DNA with molecular probes, Southern
blot , FISH
c) DNA sequencing, the preparation of DNA for sequencing
d) pyrosequencing
e) the use of methods of the genetic material analysis in
medical diagnostics
November 25, 5 DNA cloning vectors in molecular biology
2014
a) DNA cloning vectors in bacteria
b) DNA sequences useful in genetic engineering of bacteria
c) DNA cloning vectors in eukaryotic cells
d) DNA sequences useful in genetic engineering of
eukaryotic cells
e) The use of vectors in cell-gene therapy, advantages and
disadvantages
SMALL TEST 1
Student
index number
Gr A
Gr B
65180 65174
65159 65168
65170 65162
65194 Yasir
65172 65179
December 02,
2014
December 09,
2014
December 16,
2014
January 13,
2015
6 Methods for introducing DNA into living cells
a) transformation of bacteria, analysis and selection of
positive clones , the banking
b) transduction of eukaryotic cells, the pros and cons
c) transfection of eukaryotic cells, the advantages and
disadvantages
d) analysis and selection of eukaryotic cells positive clones,
reporter genes, FACS
e) the use of cloning in modern medicine
7 Possibility of repair and exchange of genetic material
a) methods of repair DNA sequences in vitro / methods of
mutations introducing
b) non-homologous recombination in cells
c) homologous recombination in cells
d) transposition of DNA
e) the use of genetic engineering in modern medicine
8 Analysis of proteins by molecular methods
a) genetic code, artificial DNA sequences important in the
process of translation and its biological significance
b) protein electrophoresis
c) western blot and other methods of enzyme immunoassays
d) chromatography
e) spectrophotometric and spectral methods for protein
analysis
9 Expression of recombinant proteins
a) conditions for a successful expression of proteins in
bacteria, the correct genetic construction, labeling of
proteins in the preparation of DNA constructs
b) colony culture conditions, used bacterial strains
c) the conditions for the effective expression of proteins in
eukaryotic cells, the correct genetic construction
d) culture conditions of eukaryotic cells, useful cell lines,
adherent cells, insect cells, plant cells, yeast cells
e) analysis and purification of the protein, useful labels
SMALL TEST 2