DNA SEQUENCE SUBMISSION FORM – INTERNAL

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DNA SEQUENCE SUBMISSION FORM – EXTERNAL
Phone: (905) 525-9140 ext. 27048
Fax: (905) 526-1427 E-mail: [email protected] http:// www.science.mcmaster.ca/mobix/
NAME:
DATE:
SUPERVISOR:
TELEPHONE # (
)
EXT.
PO# OR CREDIT CARD #
EXP.
E-MAIL ADDRESS:
 WITH ($18)
 TEXT FILE
 YES
EDITING REQUEST**
E-MAIL RESULTS
MAIL PRINTOUTS (PUROLATOR RATE)
MAILING ADDRESS
SPECIAL CONSIDERATION:
BILLING ADDRESS (if different from mailing)
1)
high GC content (add dmso)
2) difficult template (hairpin protocol)
#
Name of DNA
*MAX 8 CHARACTERS
 WITHOUT($10)
 ABI FILE
Name of PRIMER
*MAX 8 CHARACTERS
#
1
9
2
10
3
11
4
12
5
13
6
14
7
15
8
16
 YES
 YES
(may take 1 to 2 additional days)
Name of DNA
*MAX 8 CHARACTERS
Name of PRIMER
*MAX 8 CHARACTERS
SAMPLE AND PRIMER PREPARATION - (sample & primer should be suspended in Water); we require 5μl of sample and 5μl primer per reaction
**Editing is the correction of miscalled bases. These problems generally occur at the beginning and end of the sequence, but can occur anywhere.
Note – you require the ABI file and the correct computer software to do your own editing.
Sample Concentration – because sequencing is unidirectional the number of copies of your template in the sample becomes very important, thus we require minimum
concentration of DNA to perform the reaction. Too much DNA can result in a short read, or a smear, or no-signal. The sample concentrations listed below take into
account the TOTAL amount of DNA in the sample (i.e. plasmid + insert) and are concentrations calculated to encourage success.
DNA concentration required
Please indicate your
Total Sample Size
ds DNA <10 kb
100 ng/μl
approx. size
ds DNA 10kb-200kb
100ng-2ug/μl
approx. size
PCR products
1ng/μl per 100 bases
approx. size
Primer Concentration: 1 picomole/μl = [1 μM]
SAMPLE STORAGE: We keep DNA samples and primers for only one month after they have been processed.
Mobix Lab, HSC-3N4, McMaster University, 1200 Main Street. W., Hamilton, ON, L8N 3Z5
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