Sample submission form - Yerkes Regional Primate Research Center

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NHP Genomics Core Lab
SOP# 47.1
YERKES NHP GENOMICS CORE
Sample Submission Form
Please print this form and fill all the fields, fill N/A for inapplicable fields. Please ship (or
bring) your samples with copy of the filled form with your samples on dry ice to the
following address:
NHP Genomics Core Lab
Attn.: Nirav Patel
Yerkes National Primate Research Center (YNPRC)
Emory University
Genomics Core, Room#1122
954 Gatewood Road
Atlanta GA 30329
For any questions please contact Nirav Patel at Nirav.patel@emory.edu or call 404-7126898
User Information
Date
PI Name
Contact Person Name
E-mail address
Phone Number
Smartkey* #
Address
Admin person contact details, if any
*For Emory investigators only
Sample details
Number of samples
Species (e.g. Human, Rhesus, Sooty
Mangabey etc.)
Sample type (e.g. Gut biopsy, PBMC,
Whole blood, sorted cells, RNA, cDNA
etc.)
Sample quantity (e.g. number of cells,
volume of whole blood for Paxgene tubes,
size of biopsy etc.)
Sample media (e.g. RLT/BME, Snap
frozen, Nuclease free Water, other buffer
etc.)
Volume of buffer/Solution used (μl)
Comments, if any
1
NHP Genomics Core Lab
SOP# 47.1
Section 1- Assay details
Service(s) required
o Microarray (Go to Section 2)
o Next Generation Sequencing (Go to Section 3)
o Quality Control (QC) only (Go to Section 4)
o DNA/RNA extraction and QC (Go to Section 4)
Section 2- Microarray
Please circle the correct answer:
Is this a new project?
Yes/No
Is this continuation of a previous project?
Yes/No
Will you be providing Gene Chips for this project?
Yes/No
If yes, GeneChip type# _________________________
GeneChip Cat# _________________________
Is Bioinformatics service required for this project?
Yes/No
Would you like your samples after project completion? Yes/No
Section 3- Next Generation Sequencing
Please circle the correct answer:
Is this a new project?
Yes/No
Is this continuation of a previous project?
Yes/No
Is Bioinformatics service required for this project?
Yes/No
Would you like your samples after project completion? Yes/No
Experimental details
Application (e.g., RNA-Seq, DNA-Seq,
Small RNA, Directional mRNA seq etc.)
Kit used to prepare library
Sample/Library Purification method
Library Quantitation method (e.g., Qubit,
Bioanalyzer, qPCR)
Read Length (e.g., 1X50, 2X50, 1X100,
2X100, 1X36, 2X36)
Run type: ☐ SR ☐ PE ☐ SR multiplexed ☐ PE multiplexed
Number of reads/Sample required
Comments, if any
Section 4- DNA/RNA Extraction and QC
Note: For QC service, please provide a minimum of 3 μl sample for analysis.
Sample Specific details
Number of samples
Sample type e.g. RNA, dsDNA, ssDNA
etc. (for QC service only)
QC service required
☐ Nanodrop
Comments, if any
☐ Bioanalyzer
2
☐ Qubit
NHP Genomics Core Lab
SOP# 47.1
Section 5- Sample Inventory (to be filled for all services)
Please use the following template below and fill applicable column(s), submit a separate
excel sheet with details. Please note that we will perform Agilent Bioanalyzer analysis on
all RNA samples submitted for Microarray and Next Generation Sequencing services.
Sample
#
Unique
sample
ID
Sample
description
OD
260/280**
Conc.
(ng/μl)
Vol
(μl)
Species
RNA/DNA
extraction
method
1
2
** OD 260/280 ratios must be between 1.8-2.2 for Microarray and Next Generation Sequencing services.
For Genomics Core use only
Project number assigned:
Project name:
3
NHP Genomics Core Lab
SOP# 47.1
4
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