NHP Genomics Core Lab SOP# 47.1 YERKES NHP GENOMICS CORE Sample Submission Form Please print this form and fill all the fields, fill N/A for inapplicable fields. Please ship (or bring) your samples with copy of the filled form with your samples on dry ice to the following address: NHP Genomics Core Lab Attn.: Nirav Patel Yerkes National Primate Research Center (YNPRC) Emory University Genomics Core, Room#1122 954 Gatewood Road Atlanta GA 30329 For any questions please contact Nirav Patel at Nirav.patel@emory.edu or call 404-7126898 User Information Date PI Name Contact Person Name E-mail address Phone Number Smartkey* # Address Admin person contact details, if any *For Emory investigators only Sample details Number of samples Species (e.g. Human, Rhesus, Sooty Mangabey etc.) Sample type (e.g. Gut biopsy, PBMC, Whole blood, sorted cells, RNA, cDNA etc.) Sample quantity (e.g. number of cells, volume of whole blood for Paxgene tubes, size of biopsy etc.) Sample media (e.g. RLT/BME, Snap frozen, Nuclease free Water, other buffer etc.) Volume of buffer/Solution used (μl) Comments, if any 1 NHP Genomics Core Lab SOP# 47.1 Section 1- Assay details Service(s) required o Microarray (Go to Section 2) o Next Generation Sequencing (Go to Section 3) o Quality Control (QC) only (Go to Section 4) o DNA/RNA extraction and QC (Go to Section 4) Section 2- Microarray Please circle the correct answer: Is this a new project? Yes/No Is this continuation of a previous project? Yes/No Will you be providing Gene Chips for this project? Yes/No If yes, GeneChip type# _________________________ GeneChip Cat# _________________________ Is Bioinformatics service required for this project? Yes/No Would you like your samples after project completion? Yes/No Section 3- Next Generation Sequencing Please circle the correct answer: Is this a new project? Yes/No Is this continuation of a previous project? Yes/No Is Bioinformatics service required for this project? Yes/No Would you like your samples after project completion? Yes/No Experimental details Application (e.g., RNA-Seq, DNA-Seq, Small RNA, Directional mRNA seq etc.) Kit used to prepare library Sample/Library Purification method Library Quantitation method (e.g., Qubit, Bioanalyzer, qPCR) Read Length (e.g., 1X50, 2X50, 1X100, 2X100, 1X36, 2X36) Run type: ☐ SR ☐ PE ☐ SR multiplexed ☐ PE multiplexed Number of reads/Sample required Comments, if any Section 4- DNA/RNA Extraction and QC Note: For QC service, please provide a minimum of 3 μl sample for analysis. Sample Specific details Number of samples Sample type e.g. RNA, dsDNA, ssDNA etc. (for QC service only) QC service required ☐ Nanodrop Comments, if any ☐ Bioanalyzer 2 ☐ Qubit NHP Genomics Core Lab SOP# 47.1 Section 5- Sample Inventory (to be filled for all services) Please use the following template below and fill applicable column(s), submit a separate excel sheet with details. Please note that we will perform Agilent Bioanalyzer analysis on all RNA samples submitted for Microarray and Next Generation Sequencing services. Sample # Unique sample ID Sample description OD 260/280** Conc. (ng/μl) Vol (μl) Species RNA/DNA extraction method 1 2 ** OD 260/280 ratios must be between 1.8-2.2 for Microarray and Next Generation Sequencing services. For Genomics Core use only Project number assigned: Project name: 3 NHP Genomics Core Lab SOP# 47.1 4