Molecular Biology Services
RNA Sample Preparation &
Analysis Order Form
Axil Scientific Pte Ltd
41 Science Park Road, #01-22/23 The Gemini
Singapore Science Park II, Singapore 117610
T: +65 6775 7318
F: +65 6775 7211
E: mbs@axilscientific.com
First BASE Laboratories Sdn Bhd (604944-X)
Lot 7-1 to 7-3, Jalan SP 2/7, Taman Serdang Perdana,
Seksyen 2, 43300 Seri Kembangan, Selangor, Malaysia
T: +603 8943 3252
F: +603 8943 3243
E: mbs-my@base-asia.com
* denotes required fields
CUSTOMER DETAILS
*Name:
Reference ID:
*Institute Name / Department
*Address:
*Contact Number:
*Email Address:
Principal Investigator / Supervisor:
(Office)
(Mobile)
RNA SAMPLE PREPARATION / ANALYSIS (Please indicate with a X)
Type of Service
A.
PREPARATION
Reverse Transcription PCR (RT-PCR):
First-strand cDNA (cDNA/RNA heteromer) synthesis by oligo(dT)
RNA Extraction from Tissue Sample
Please indicate amount of Tissue Sample
mg
(This service fee includes extraction up to 100mg of tissue, please multiply
accordingly service fee with every 100mg of starting material.)
RNA LabChip:
Total RNA (25 to 500 ng/l), Eukaryotic, 18S and 28S, size 25 - 6000 bp
B.
ANALYSIS
RNA LabChip:
Total RNA (25 to 500 ng/l), Eukaryotic (Plant), 18S and 25S, size 25 - 6000 bp
RNA Labchip:
Total RNA (25 to 500 ng/l), Prokaryotic, 16S and 23S, size 25 - 6000 bp
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Min. sample amount / reaction
required
I. 10 g of total RNA
II. OD260/280 = 1.8-2.0
III. Gel photo for total RNA
I. Service includes extraction up to
100mg of tissue/tube. Please
multiple accordingly.
II. Store at minus 80oC/dry ice
Remarks
I. 5 l RNA
II. Concentration: 25 to 500 ng/l in
RNase-free water
I. 5 l RNA
II. Concentration: 25 to 500 ng/l in
RNase-free water
I. 5 l RNA
II. Concentration: 25 to 500 ng/l in
RNase-free water
Salt (TRIS) 10mM or
higher can interfere
with analysis
Salt (TRIS) 10mM or
higher can interfere
with analysis
Salt (TRIS) 10mM or
higher can interfere
with analysis
Molecular Biology Services
RNA Sample Preparation &
Analysis Order Form
Axil Scientific Pte Ltd
41 Science Park Road, #01-22/23 The Gemini
Singapore Science Park II, Singapore 117610
T: +65 6775 7318
F: +65 6775 7211
E: mbs@axilscientific.com
First BASE Laboratories Sdn Bhd (604944-X)
Lot 7-1 to 7-3, Jalan SP 2/7, Taman Serdang Perdana,
Seksyen 2, 43300 Seri Kembangan, Selangor, Malaysia
T: +603 8943 3252
F: +603 8943 3243
E: mbs-my@base-asia.com
SAMPLE INFORMATION (Note: please attach a separate sheet with details as requested in the table if submiting more than 5 samples)
S/N *Sample Name
Organism
Tissue weight
(mg)
Concentration
(ng/l)
Purity
OD260/280
Volume
(l)
#
Special Instructions/Protocols/ Result Requirements
1.
2.
3.
4.
5.
* Required fields
# Please indicate if you have any specific instructions/protocols/result requirements, attach as separate sheets if necessary
Have I met the sample preparation requirements?
Checklist (Please indicate with an X):
Submitted minimum required sample amounts for one reaction.
Purified total RNA is in RNase-free water. Its purity OD260/280 = 1.8-2.0. For RT-PCR service, please attach gel photo of total RNA.
Samples submitted in 1.5/ 0.5ml microcentrifuge tubes. Each tube should be clearly labeled using a permanent marker, with caps sealed with parafilm.
RNA samples should be shipped at -20oC to prevent sample degradation.
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