CHEM 7880
MACROMOLECULAR CRYSTALLOGRAPHY
Spring 2015
INSTRUCTOR
Prof. Steven E. Ealick <see3@cornell.edu>
Department of Chemistry and Chemical Biology
382A S. T. Olin Chemistry Research Laboratory
WEB SITE
Leslie Kinsland <lk65@cornell.edu>
http://arginine.chem.cornell.edu/CHEM7880/Chem7880.html
Requires Cornell user ID and password
GRADING
Weekly take home problems (20%), two in class prelim exams (20% each), final paper
(40%). Weekly problems will be handed out on Thursdays and will be due the following
class.
Prelim exam 1 is March 5
Prelim exam 2 is April 9
Final paper is due on May 15
GRADER
Dr. Yang Zhang yz46@cornell.edu
377 S.T. Olin Chemistry Research Laboratory
TIME AND PLACE
Tuesdays and Thursdays, 1:25 - 2:40 P
Baker Laboratory 335
TEXTBOOK
Gale Rhodes, Biomolecular Crystallography Made Crystal Clear, 3rd Edition
ISBN 978-0-12-587073-3
COURSE DESCRIPTION
Protein X-ray crystallography is becoming increasingly automated. This course will
emphasize the current state of three-dimensional structure determination of
macromolecules using X-ray crystallography. The students will be guided through the
steps that are involved with an emphasis on practical aspects.
COURSE SCHEDULE
1.
January 22 (Th): Introduction and overview
2.
3.
January 25 (Tu): Protein structure
January 27 (Th): Crystallization
4.
5.
February 3 (Tu): Crystallography basics
February 5 (Th): Beamline instrumentation
6.
7.
February 10 (Tu): Becoming a SR user (Dr. Raj Rajashankar)
February 12 (Th): Solving a structure at the beamline (Dr. Raj Rajashankar)
February 17 (Tu): February Break
February 19 (Th): no class
8.
9.
February 24 (Tu): Crystals, lattices, and symmetry
February 26 (Th): Space groups
10.
March 3 (Tu): Reciprocal space
March 5 (Th): Prelim 1
11.
12.
March 10 (Tu): Diffraction basics
March 12 (Th): Phasing methods
13.
14.
March 17 (Tu): Data collection (Dr. Frank Murphy)
March 19 (Th): Data processing (Dr. Frank Murphy)
15.
16.
March 24 (Tu): Model Building (Dr. Jon Schuermann)
March 26 (Th): Refinement and validation (Dr. Jon Schuermann)
March 31 (Tu): Spring Break
April 2 (Th): Spring Break
17.
April 7 (Tu): Reading an X-ray crystallography paper
April 9 (Th): Prelim 2
18.
19.
April 14 (Tu): Finishing the structure, a guide to CCP4 (Dr. Ivo Tews)
April 16 (Th): Complementary methods, a case study (Dr. Ivo Tews)
20.
April 21 (Tu): Pitfalls and validation
April 23 (Th): NE-CAT remote data collection (Dr. Frank Murphy)
April 28 (Tu): no lecture - students work in groups to solve and refine structure
April 30 (Th): no lecture - students work in groups to solve and refine structure
21.
May 5 (Tu): Wrap up, course evaluation