Social Networking in Lizards: Flinders Workshop Students: Primary and Secondary Context: Will have 2 presentations - Static display - 45 mins workshop Student Outcomes Static display: Lizards live in family groups – social networks DNA and behavioural observations are used to gather data Workshop: 1. DNA Workshop Demonstrate a DNA extraction Discuss interpretation of gel profiles Link into building picture with ecological observations 2. Social Network Workshop Students participate in making a social network Discuss how to record a network Link the importance of this research to building a healthy ecosystem Workshop Whole group (5 mins) Learning Task Welcome! Resources Today we are going to look at what some scientists at Flinders University do. First tell me what you know about scientists? (ask 2 -3 kids). Did you know that some scientists at Flinders University study lizards? Now who has watched documentaries on TV? Chances are you would have seen David Attenborough (pp pic of Mike Bull with David Attenborough). The work done at Flinders University was so novel that he came and filmed Flinders University scientists work. Now what we are doing today is… We will have 2 activities…discuss in brief the workshop (DNA and how we interpret data. Gidgee skinks (star of the show) and how we have interpreted their DNA…so cool world first.…we will make a social network activity. Really cool, SA, Flinders leading the world in this research). What is so unusual about this? Ask kids who has seen lizards in the wild/or their backyard? Were they in groups? Most lizards are solitary, but sometimes can be found in groups. DNA Workshop (10 mins) Intro: Let’s get straight into it… historically the main way of gathering evidence was by observation …explain we paint lizards to tell apart from a distance…but with new tech….bit like being a detective and piecing the puzzle together. What do you think DNA is? /What do you know about DNA? Put your hands up…. Thank you for your idea/thinking. (only ask 3 kids max) Show video (?). http://www.neok12.com/php/watch.php?v=zX5e724c6f0042150202670a&t=Genetics (2:25 mins) Key point: DNA provides all the info on how to build any living things DNA is used to tell individuals apart. Discuss where do I get DNA from a lizard? Do I need to kill this lizard to get its DNA? Ask 2 kids max. Show pp pic Lead discussion to why you are doing a pea extraction and not a lizard extraction because….(not enough DNA to see from a lizard) Powerpoint PP and Extraction protocol on the website Lizard bookmark with web details Lizards Extraction stuff and table Activity: (Prepare just before workshop) Walk through the pea DNA extraction step by step in the PP. Step 1 Put peas into blender and mash up (breaks cells apart) Step 2 Strain and add detergent (causes the cell membrane to break down) Step 3 Add meat tenderiser (enzymes - releases DNA into solution) Step 4 Alcohol separation (makes DNA clump together) No volunteers for except for the adding of alcohol at the end (need to have prepared this solution before start of workshop) Not the end of the story…show how the gels are run. Use pp pic Show a gel with 3 lizard profiles. Can anyone explain what they think the peaks mean? Discuss what the peaks mean. Sec level: Discuss regions of DNA - microsatellites Conclusion: Cut any discussion (see you at end of workshop if any questions) To wrap up say the key points of the lesson: - we know where to get DNA from - we extracted DNA - got a bit of an idea on how to read a DNA profile Now we know how to get DNA, I want to take you back to the start of the show which is the gidgees. As I said, we have found that the gidgees live in family groups – so I want to show you how we worked that out. So before we do that it might be good to have a little break and we are going to get out the gidgee and let you guys have a look. Special request – they are live animals so you have to be careful and not too noisy. (To get DNA info…website given to teachers for the kids to go on line or p/c at the back of the room.) Energiser (3 mins MAX!) Stand up and wonder through with the gidgees (2 people one each) Social Intro: Networking Now we know that some of the lizards were related by looking at their (20 mins) DNA (just like we did)…the next obvious question was HOW are these lizards related? Is it just dad/mum and the kids, or are there aunties/uncles /cousins, grandmas etc…? (Slides mum and child…brothers) Intro tool of social networks. Who has heard of social networking? Any ideas what it means? Who uses Facebook? What the point of using Facebook? (Lead discussion to key point of being connected or SHOWING CONNECTIONS) Network cards 5 cards primary level 7 cards secondary level Flagging tape cut to the correct length Show pic of gidgee network We discovered that these lizards were not that dissimilar…they have their own social network. Today we are going to see how scientists try working out the network. It is a lot harder than humans because Lizard these lizards are so secretive. hats Activity: What we are going to do and make our own gidgee skink social network. Explain activity. Call for 8 volunteers – give first one their card, they read card aloud. Progress thru cards each time getting the volunteers to stand in spot as called. Work through cards and flagging tape. (Tip: Make sure when handing the flagging tape to the kids to show the connections remind them to hold flagging tape carefully) Once all positioned – get the chn to sit where they are and not let go of the flagging tape. Tip: Masking tape to mark out positions on floor This is not the end of the story, once observations made…now we have to record it….construct a network. Ask a teacher for a volunteer who they think would be good for this job. On whiteboard student to draw network. Once network drawn audience to give a clap- collect cards and rope and send all students back to their seats. Lead wrap up by discussing the network picture Primary level: keep relatively simple As you can see by this network diagram we have a much better understanding of what is happening in the groups. …I am going to ask a question… What would it mean if a parasite was to infest Lizard 1? (low connection) Lizard 2 (high connection)? What do you think would happen? Through which lizard would the disease spread fastest? OK we have highlighted a bad scenario living in groups (parasite spread), can you think of why living in groups would be advantageous? - predator avoidance / vigilance - sharing common resource Great thinking…I suppose the point of all this is that as scientists we need to know how the lizards are interacting to know how the parasites are spreading so that we can learn how to stop the parasite spread if a new parasite gets into the system. Why is this important to know? Relocation (climate change/avoid extinction); health of ecosystem; fostering a healthy biodiversity. Conclusion: To wrap up say the key points of the lesson: - lizards live in networks - these networks highlight good and bad knock on effects - need to monitor to ensure a healthy eco system. Depending on time…You have been fantastic listeners…Any questions? Next slide: Any questions To get info…website given to teachers for the kids to go on line. Hand out freebies (fridge magnets). Brochures for courses (public groups) Finish Informal chat On website: (Make it very kid friendly – lots of images) - A special front cover page where you can click on either workshops for lesson outline / pp / extraction details / network cards / DNA origami template / links to you tube - Links for further info - Maybe photos of the workshops - Keep live for a month after the workshop - Career pathway options for this kind of career at Flinders DNA Extraction: Genetic Science Learning Center (2011, January 24) How to Extract DNA from Anything Living. Learn.Genetics. Retrieved August 4, 2011, from http://learn.genetics.utah.edu/content/labs/extraction/howto/