How to create a program on the Paradigm plate reader
Step 1. Ensure the PC and plate reader are on, if there is a yellow light on the plate
reader it is in STANDBY.
Step 2 From the Windows Start menu, select Programs | Beckman Coulter |
Multimode Analysis Software | Multimode Analysis Software. The Multimode
Analysis Software window displays.
Do you already have a protocol? YES go to “How To Run a Program on the
Paradigm”
Step 3 Select
protocols. Click
from the navigation pane. This will display a list of all
from the tool bar.
Step 4 There are two options for creating protocols, New Protocol and Template
Protocols. To create a new protocol select the New Protocol tab and continue to
step 5. To create a protocol using a template protocol see Creating a Protocol from
a Template on page 7-187.
Step 5 Select the Protocol Type:
Analysis - Analysis applications allow measurement data to be transformed and
analyzed using formulas, variables, and parameters configured in the protocol.
Quantitation - Quantitation applications measure the purity and/or concentration of
proteins or nucleic acid samples, such as DNA or RNA.
Step 6 Click Next to configure the protocol.
Protocol name, enter a unique name for the protocol.
Duplicate protocol names are not permitted.
Notes, enter a description for the protocol, if desired. 3.
Select the desired Analysis Options for configuration in the protocol:
Variables - define up to ten numeric values that may be used in any formula
configured in the protocol. NOTE The Variables option is only
available for Analysis applications.
Transformation - configure an algebraic formula to transform measurement
data
Concentration - values or formulas for quantitation of sample concentration
with a standard curve.
Cutoff - configure cutoff formulas to classify measured samples according to
defined cutoff values.
Click Next to select the Technique Type
Step 7 Technique Type, select the desired measurement technique(s). Multiple
techniques may be selected to create protocols using different detection techniques.
Click Next to select the Labware Type
Step 8 Select the desired Type of Labware from the list. Click Next to configure the
Labware Layout Settings (for layout settings see page 7-145 Multimode Software
User’s manual on sharepoint).
Step 9 Click Next to configure detection and preparation methods, see page 7-150
Multimode Software User’s manual on sharepoint).
Step 10 Click Next to configure data reduction methods, see page 7-169 Multimode
Software User’s manual on sharepoint).