NEW YORK UNIVERSITY SCHOOL OF MEDICINE Division of Laboratory Animal Resources (DLAR) April 30, 2014 One Park Ave, 6 th floor, 6-221C New York, NY 10016 DLAR Laboratory Animal Training Program Instructions Training is important for the appropriate care and use of laboratory animals. It is mandated by the Animal Welfare Act (9 CFR Chapter 1, Subchapter A, Subpart C, Section 2.32). All researchers are required to participate in laboratory animal training program in order to receive access to the animal facilities of NYU School of Medicine/Medical Center. GUIDELINES TO ACCESS THE ANIMAL FACILITIES – All new Applicants for Skirball (SCAF), Dental (DCAF), Parasitology (PCAF), Sloan Kettering (MSCAF), Varick (VCAF) , Satellite Animal Facilities, Sterling Forest (DEMSAF) and Alexandria ( ERSP) The orientation includes a brief overview of NYUSoM Animal Facilities, the laws and regulations pertaining to animal research, information on animal ordering, DLAR husbandry services, DLAR veterinary services, Guide to Occupational Health and Safety, the availability and charges for special services, and per diem rates. Any questions pertinent to specific animal research programs in the investigator's laboratory are addressed at this time. Please follow the steps below: 1. Obtain an ID card: a. an NYU Langone Medical Center ID is needed for SCAF, PCAF, DEMSAF, VCAF and Smilow and Skirball Satellites. b. an NYU Dental School ID is needed for DCAF at Washington Square, c. a Sloan Kettering/MSCAF ID is needed at MSKCC. For information, refer to DLAR’s website at www.med.nyu.edu/dlar, see Memorial Sloan-Kettering Animal Facility on the home page. You will need to login with your Kerberos ID and password for access to the website. d. an Alexandria/ERSP ID is obtained thru Mispro Biotech, please email: kenneth.jones@misprobiotech.com The Principal Investigator (PI) or Lab Manager can assist the new research staff to obtain an ID card from respective facility sites. 2. Obtain a Kerberos ID: Contact MCIT Helpdesk at 212-263-6868. Employees are issued a Kerberos ID upon starting a job at NYULMC. The PI or the Lab Manager can assist in obtaining the Kerberos ID. Kerberos ID and its password are needed to perform CITI web-based animal training. 3. Submission of Forms: Bring the completed forms to the Skirball Animal Facility Supervisor’s office (ground floor of Skirball building). See page 6 and 7 of this packet. 1) Registration Form 2) Animal Facility Security Access Form (with PI’s signature) All “Forms”” must be submitted together. Deadline for submission is the Monday on the week of DLAR orientation at 3 pm. Once the forms are submitted, the Training Coordinator will review the forms for proper completion and email the applicants to confirm their attendance to the IACUC/DLAR lecture orientation. 1 of 8 4. Any researchers may attend the orientation prior to protocol approval, but a tour is given ONLY after receiving the approval. Please contact IACUC regarding protocols: iacuc@nyumc.org Attend the IACUC/DLAR orientation: This lecture starts promptly at 9 am and ends at 10:30 am. In general, regulations on the conduct of animal research, the role of the IACUC, care and use of lab animals in the facility will be presented. All participants are encouraged to arrive at the lecture room prior to 9:00 a.m. There are no late admittance. 2014 Begins promptly 9-10:30 am May 7 May 21 June 4 June 18 July 2 July 16 July 30 Aug 13 Sept 10 Sept 24 Oct 8 Oct 22* Nov 5 Nov 19 Dec 3 Dec 1 7 Smilow Conf room B at the end of lobby Aug 27 Location: Smilow Seminar Room, except where indicated * 5. Any researchers may complete the CITI web-based animal training module, instruction is below. Please contact IACUC regarding CITI: iacuc@nyumc.org Complete the required CITI web-based animal training module. INSTRUCTION: Go to Collaborative Institutional Training Initiative (CITI) https://www.citiprogram.org/Default.asp Use NYU School of Medicine/NYU Langone Medical Center as your affiliate institution (Do not use NYU). The system requires you to use your Kerberos ID and password associated with it. Contact CITI (Helpdesk 305-243-7970), if problems occur. All researchers MUST take the web animal training mandatory courses (modules) as follows: A. Complete the MANDATORY COURSES for all Researchers 1. “Working with the IACUC” 2. “Species-Specific Module” Researchers will select species- specific modules depending on which lab animals are to be used, e.g. working with Frogs, Toads or other Amphibians, Mice, Rats, Hamsters, Gerbils, Guinea Pigs, Rabbits, Dogs, Swine or Pigs , Nonhuman primates , etc. 3. “Minimizing Pain and Distress”. ALL users are required to complete this module. B. If researchers are conducting survival surgery on MAMMALS, “Aseptic Surgery” module is required. C. For IACUC members ONLY, please complete “Essentials for IACUC members course” D. OPTIONAL Refresher Course: “Working With Animals in Biomedical Research” Refer to http://iacuc.med.nyu.edu/training see Training, for CITI tutorial. 6. Attend the hands-on training: This is required for applicants who have no experience or limited experience in handling mice, rats, guinea pigs, hamsters, rabbits and other species of lab animals. Those who have signified experience for a year or more may not attend the hands-on training, unless the applicant voluntarily expresses intention to attend as a refresher-training. The regular schedule of hands-on training usually occurs on the Friday 2 of 8 of orientation week. Take note that hands-on training is required before a minor amendment of the research protocol can be approved by the IACUC. Only personnel included as Protocol Associates in an approved research protocol can access the animal facility, and therefore, are required to have a tour of the animal facility. “Rodent Biomethodology” hands-on training workshop 1. Refer to the schedule below. 2. Register for a particular date. 3. Confirmation will be emailed to the applicant once it is received. 4. Applicants can also email the Training Coordinator (cortec03@nyumcorg) to follow-up on the schedule. 5. Bring a copy of Biomethodology Hand-out, see www.med.nyu.edu/dlar, Training & Education, Rodent Seminar & Rodent Hands-on Workshops/ handouts 2014 Rodent Hands-on Workshop schedule Workshops are usually the same week as the orientation. May 9 July 18 Sept 12 Nov 7 May 23 Sept 26 Nov 21 Aug 1 June 6 Aug 15 Oct 10 Dec 5 June 20 Aug 29 Oct 24 Dec 19 Registration is required, registration form is on page 8 of this packet and also available on the DLAR website: www.med.nyu.edu/dlar training&education, Hands-on Workshop Occurance: dates above Time: 1:30-2:30 pm Location: 11th Floor of Dental Animal Facility. The building is located along 1st Avenue between 24th and 25th street. The main entrance is on 24th street. Upon entering the main lobby, you will find 4 elevators, take the SL (sublevel) elevator by going downstairs to the sub-level. This is an an express elevator and will only stop twice, on the 6th and on the 11th floor. Get off on 11th floor , turn LEFT, then turn RIGHT and turn LEFT, you will see a door. Knock on the door or call the DLAR office at 212-998-9982, this door will be opened for you. You may also take any of the 3 elevators in the lobby, but it will stop on each floor. For your reference, download the “Biomethodology Handout” from the DLAR website: www.med.nyu.edu/dlar ; see Training & Education For any questions, please contact the Trainer at cortec03@nyumc.org or tel. 646-5012282. 7. Tour the animal facility: A tour will be given after all the above is completed and only after the researcher’s name is added to the approved protocol. Applicants are encouraged to follow-up on the approval of the research protocol thru their PIs and Lab Managers, then, contact the respective Facility Supervisors once the protocol is approved, to attend the tour of the facility. The Training Coordinator and Facility Supervisors with IACUC validation, will countercheck each approved protocol in order for the applicants to participate on the tour. 3 of 8 A checklist of the tour is found on the DLAR website www.med.nyu.edu/dlar, see training & education, required orientation & tour, applicants should bring a copy of the checklist for the tour. Please contact the Facility Supervisor to schedule a tour at the email below: a. For Sloan (MSCAF) tour, email: abdoul.fall@nyumc.org Sloan access will be coordinated thru the DLAR Operations Manager (brian.imperiale@nyumc.org), refer to DLAR homepage Memorial Sloan Animal Facility for detailed instruction. b. For Skirball (SCAF) tour, email: Lillian.rodriguez@nyumc.org, users will bring the Animal Facility Access Form signed by the Facility Supervisor after the tour, to MSB 182, Security Office for activation of ID card. c. For Dental (DCAF) tour, email: frank.mercogliano@nyumc.org Dental access will be arranged by DLAR Operations Manager, DLAR Operations Manager (brian.imperiale@nyumc.org) after the tour with Washington Square Security. d. For Parasitology (OPH), Satellites, and Varick (VCAF), email: frank.mercogliano@nyumc.org, users will bring the Animal Facility Access Form signed by the Facility Supervisor after the tour to MSB 182, Security Office for activation of ID card. e. For Sterling Forest (DEMSAF), email Dr. Tchou-Wong: Kam-Meng.TchouWong@nyumc.org f. For Alexandria (ERSP) email: kenneth.jones@misprobiotech.com Note: Email or contact the Supervisors/Managers regarding a Tours ONLY when your protocol has been approved and your name was added. Facility tours are only routinely scheduled for rodent users as stated above. If you are working with a different species, contact the Training Coordinator for an appointment. Prior to a tour for a different species, the animal users must have their names included in an approved IACUC protocol, and will be arranged by the Facility Supervisor/Training Coordinator. Follow up the approval of the protocol with your PI or Lab Manager. IACUC usually sends an approval letter from a minor amendment of the protocol. 4 of 8 Activate your ID card Following the facility tour, the Training Coordinator or Facility Supervisor will sign the Security Access Form for access into an animal facility, the researcher will need to go to the Security Office located at MSB 182 to activate their ID card or as instructed by the Operations Manager. Security is in charge of your access into the animal facility and DLAR is in charge of your access into the animal room. Depending on the facility, a PIN code will be inputted into the DLAR security system by the Facility Supervisor for this purpose. Request to Change Animal Facility or Changing PI Contact the Training Coordinator for detailed instruction and schedule of tour. Fill-out Animal Facility Security Access Form, with the PI’s signature. Schedule tour with Supervisor via email, bring this form to the tour. The Facility Supervisor or Training Coordinator will contact you to schedule. If you have attended the orientation prior to November of 2011, from the date of request, your application will be treated as a New Applicant. DLAR Seminars Applicants are encouraged to participate in DLAR Seminars. Topics include: Rodent Breeding 101 and 102, and Aseptic Rodent Surgery Techniques. See the DLAR website: www.med.nyu.edu/dlar, see Training & Education/ seminar *****TRAINING INSTRUCTION ENDS HERE**** **************Forms are below this point.************** 5 of 8 Form 1: DLAR Orientation Registration Form Instructions: 1. Use one (1) form per applicant. 3. Submit this form to Skirball animal facility office at 3:00 pm, on the Monday before orientation date, which is a Wednesday. Confirmation to attend the orientation will be acknowledged via email. Required Information for All Applicants First Name: Last Name: KERBEROS ID: Department: Email Address : Phone #: PI Name: Protocol Number : Date: check the box if the protocol is approved & your name is on it For all Researchers: (1) Attendance to DLAR/IACUC lecture orientation, (2) completion of mandatory CITI web-based animal training modules, and (3) participation to hands-on training (if applicable) are required prior to approval of the protocol by IACUC. Tour of the animal facility is provided after the applicant’s name is included in an approved protocol. Please check your response to the questions below: I have had a pre-employment physical examination at NYULMC Occupational Health Services: YES Date__________ NO I have submitted an annual health questionnaire to the NYULMC Occupational Health Services: YES Date_________ NO I am employed at NYULMC . YES Date started: ________________ If your answer is NO, what is your status? VOLUNTEER NO OTHER specify ________________________ Please check your response to the question: Do you have experience working with rodents? No experience Limited experience > one year experience If you are a new animal user or with limited experience, you are required to attend the hands-on training workshop, “Biomethodology ” .. Email dlar@nyumc-org for registration. Refer to http://dlar.med.nyu.edu/training-and-education), for schedules & registration. Required Information for Orientation and Facility Tour (check box) Gender: Male Female Facility you need access to: Dental Skirball Sloan Kettering Parasitology-OPH Varick Satellite Others__________________________ Animal Facility access is granted 24 hours a day, 7days a week. Are you transferring to another facility or PI? YES NO If yes ,indicate previous facility ______________________________ and PI ____________________________________ to new facility _____________________________________and PI ____________________________________________ 6 of 8 Form 2: Animal Facility Security Access Form Investigators and research staff requiring access to Animal Facilities must complete and submit this form for approval. Security access is granted only to those individuals who are either NYU Langone Medical Center employees or students. This form will be processed after completion in DLAR/IACUC lab animal training program. FIRST NAME: _______________________________ LAST NAME: ____________________________________________ KERBEROS ID:_______________________________ DATE: _________________________________________________ Department: _______________________________ Protocol #:_____________________________________________ Phone number: _____________________________ Email: ________________________________________________ PI’s NAME : _________________________________ SIGNATURE OF PI: _____________________________________ ******************************************************************************************************* Acknowledgement: I am aware that I will be given access to only one animal facility. Parasitology/OPH (PCAF): Room # ________ Sloan Kettering (MSCAF): Room# _______ Skirball (SCAF): Room # __________ Varick (VCAF): Room #___________ Dental (DCAF): Room#_________ Sterling Forest (DEMSAF)___________ Satellite Facility: Room# ____________________ **************************************************************************************************************************** Same Facility Transfer: ________________________Animal Facility. From: room number ___________to: room number___________ Different Facility Transfer from ________________________ in __________Facility: TO ________________________ in _______________Facility. (Name of previous PI) (Name of new PI) ********************************************************************************************************************** FOR DLAR OFFICE USE ONLY (Please don’t write anything here) _______ Attended IACUC/DLAR general lecture orientation Attested by: ___________________________________________ Training Coordinator _________Completed all CITI mandatory modules Date: _______________________________ *********************************************************************************************************** NOTE: Only animal users whose names are included in a protocol APPROVED by IACUC will be given a tour of and access to the animal facility. _______ Attended the facility TOUR Dental School (DCAF) ________________ Skirball Facility (SCAF)________________ Satellite Facility _____________________ Sloan Kettering (MSCAF)_______________ Attested by: ________________________________ (Facility Supervisor) Parasitology (PCAF) ___________ Varick (VCAF) ________________ Date_____________________________________ 7 of 8 DLAR HANDS-ON TRAINING REGISTRATION FORM (ATTENDANCE SHEET) REQUIRED INFORMATION FOR APPLICANT FIRST Name: LAST Name (Surname): KERBEROS ID: Department: Email Address : Phone #: PI Name: Protocol Number : Your assigned animal facility: Date of application: Hands-on training workshop will cover proper handling and restraint of animal, intraperitoneal injection , CO2 euthanasia and secondary method (cervical dislocation, decapitation, thoracotomy) Please check your response to the questions below: Did you work with laboratory animals before? YES NO If yes, what species?____________ Did you work with laboratory animals before, but have limited experience? YES NO If yes, what species_______? Did you have prior experience with lab animals (> 1 yr) but would like to attend as refresher training? YES NO Biomethodological procedures to be requested: Email cortec03@nyumc.org to schedule future trainings Blood collection Injection Oral gavage ID Gender/Age determination Other procedures, specify: _____________________________________ Signature of Applicant: _______________________________ Date of hands-on training:____________________ FOR DLAR TRAINER ONLY (leave this blank) Assessment of training : Comments: Species: ___________________________ 1. Proper handling/restraint 2. CO2 euthanasia + cervical dislocation IP injection Others Attested by : _____________________________________ Date:________________________ ( Trainer) CLavilla: form modified 3/27/2014 8 of 8