Chemistry 230
Analytical Biochemistry
Spring 2006
Lecture: MWF 12 noon; 8 Salem Hall
Lab: Mon 2-5:30; 8 Salem Hall
The principal goal of this course is to understand how biological samples
are isolated and analyzed. Students will develop laboratory skills in
sample preparation and quantitative analysis.
Instructor: Dr. Rebecca Alexander
email: alexanr@wfu.edu
Office: 108 Salem Hall (758-5568)
Class web site: www.courseinfo.wfu.edu (Course ID# S2006_CHM230C)
Office hours: Tuesdays & Thursdays 1-3. Feel free to stop by my office any time; if I can’t
give you enough time we can schedule an appointment. Please come see me if you are having
trouble. If you have a disability that may require an accommodation for taking this course,
please contact the Learning Assistance Center (758-5929) within the first two weeks of the
semester.
Text: The text is “Chemical Measurements in Biological Systems” by Stewart & Ebel (Wiley
Interscience 2000).
Attendance/ Academic integrity: Class attendance is required. No make-up labs will be
provided. If documented illness or other excused absence causes you to miss a lab, your
grade will be adjusted accordingly. This only applies to excused absences; unexcused
absences will result in a zero grade for that lab. All students are expected to abide by the
Wake Forest University Honor system.
Grading:
Grading will be on the 10-point scale with plus/minus used in borderline cases.
90-100% = A (A 90% guarantees an A of some flavor – may be an A-!)
80-89% = B
70-79% = C
60-69% = D
Laboratory – conduct, assignments, reports
Quizzes (in-class and on-line)
Homework & other assignments
In-Class Test I (tentative: Mon., Feb. 6)
Final (Comprehensive) Examination (Fri., Mar. 3)
Total
125 points
50
75
100
150
500 points
25 %
10 %
15 %
20 %
30 %
Approximate schedule of lectures
CHM230 Spring 2006
1/11
1/13
1/16
1/18
1/20
1/23
1/23
1/25
1/27
1/30
1/30
2/1
2/3
2/6
2/6
2/8
2/10
2/13
2/13
2/18
2/20
2/20
2/22
2/24
2/27
3/1
3/1
3/3
Introduction to analytical biochemistry, sampling exercise (Ch. 1)
Concentration units, error (Ch. 1, App. 2, 3) – HW1
MLK Day – no class
Error analysis, statistics, Excel (App. 2, 3)
Acid-base equilibrium and pH (Ch. 2) (Candy homework due)
Buffers, Henderson-Hasselbalch (Ch. 2) (Concentration quiz)
Lab 1: Redox titration of Vitamin C – Lab report
Spectroscopy basics (Ch. 3) Luminol reaction demo
Spectrophotometric analysis and Beer’s Law (Ch. 3)
Lab 2: Buffer preparation and pKa determination
Colorimetric assays (Ch. 4)
Reaction kinetics (Ch. 4)
Midterm exam
Lab 3: Colorimetric determination of proteins – Lab report
Enzyme basics (Ch. 5)
Graphical treatment of enzyme kinetics (Ch. 6)
Enzymes in analytical assays (Ch. 5) (Kinetics quiz)
Lab 4: Kinetics
Chromatography (Ch. 7)
Chromatography (Ch. 7)
Lab 5: Ion exchange chromatography and TLC of amino acids
Electrophoresis (Ch. 8)
Ligand binding/ELISA (Ch. 9) (Separations quiz)
Method selection (Ch. 11)
Lab 6: Isolation and electrophoresis of DNA
Analytical biochemistry and forensics
Final exam