Orna Elroy-Stein, Ph.D.
Tel Aviv University
PUBLICATIONS
1. Levanon, D., J. Leiman-Hurwitz, N. Dafni, M. Wigderson, Z. Laver-Rudich, E. Danciger,
O. Elroy-Stein, Y. Groner. (1985) Architecture and anatomy of the chromosomal locus in
human chromosome 21 encoding the Cu/Zn-superoxide dismutase. EMBO J. 4, 77-84.
2. Groner, Y., O. Elroy-Stein, Y. Bernstein, N. Dafni, D. Levanon, E. Danciger, A. Neer.
Molecular genetics of Down's syndrome: Overexpression of transfected human Cu/Znsuperoxide dismutase gene and the consequent physiological changes. New York, Cold
Spring Harbor Sym. Quant. Biol. Vol.51, 1986 (pp.381-393).
3. Elroy-Stein, O., Y. Bernstein, Y. Groner. (1987) Overproduction of human Cu/Znsuperoxide dismutase in transfected cells: Extenuation of paraquate-mediated cytotoxicity
and enhancement of lipid peroxidation. EMBO J. 5, 616-622.
4. Epstein, C.J., K.B. Avraham, M. Lovett, S. Smith, O. Elroy-Stein, G. Rotman, C. Bry, Y.
Groner. (1987) Transgenic mice with increased Cu/Zn-superoxide dismutase activity:
Animal model of dosage effect in Down's syndrome. Proc. Natl. Acad. Sci. USA 84, 80448048.
5. Elroy-Stein, O. and Y. Groner. (1988) Impaired neurotransmitter uptake in rat PC12 cells
overexpressing human Cu/Zn-superoxide dismutase - Implication for dosage effect in
Down's syndrome. Cell 52, 259-267.
6. Schickler, M., H. Knobler, K.B. Avraham, O. Elroy-Stein, Y. Groner, (1989) Diminished
serotonin uptake in platelets of transgenic mice with increased Cu/Zn-superoxide dismutase
activity. EMBO J. 8, 1385-1392.
7. Elroy-Stein, O., T.R. Fuerst, B. Moss. (1989) Cap-independent translation of mRNA
conferred by encephalomyocarditis virus 5' sequence improves the performance of the
vaccinia virus/bacteriophage T7 hybrid expression system. Proc. Natl. Acad. Sci. USA 86,
6126-6130.
8. Groner, Y., O. Elroy-Stein, K.B. Avraham, R. Yarom, M. Schickler, H. Knobler, G. Rotman.
(1990) Down syndrome clinical symptoms are manifested in transfected cells and transgenic
mice overexpressing the human Cu/Zn-superoxide dismutase gene. J. Physiol. 84, 53-77.
9. Elroy-Stein, O. and B. Moss. (1990) Cytoplasmic expression system based on constitutive
synthesis of bacteriophage T7 RNA polymerase in mammalian cells. Proc. Natl. Acad. Sci.
USA 87, 6743-6747.
10. Moss, B., O. Elroy-Stein, T. Mizukami, W.A. Alexander, T.R. Fuerst. (1990) New
mammalian expression vectors. Nature 348, 91-92.
11. Zibert, A., H-C. Selinka, O. Elroy-Stein, B. Moss, E. Wimmer. (1991) Vaccinia Virus
mediated Expression and Identification of the Human Poliovirus Receptor. Virology 182,
250-259.
12. Yang, X-C., A. Karschin, C. Labarca, O. Elroy-Stein, B. Moss, N. Davidson, H.N. Lester.
(1991) Expression of ion channels and receptors in Xenopus oocytes using vaccinia virus.
FASEB J. 5, 2209-2216.
Orna Elroy-Stein, Ph.D.
Tel Aviv University
13. Karschin, A., B.Y. Ho, C. Labarca, O. Elroy-Stein, B. Moss, N. Davidson, H.A. Lester.
(1991) Heterologously expressed serotonin 1A receptors couple to muscarinic K+ channel
in heart. Proc. Natl. Acad. Sci. USA. 88, 5694-5698.
14. Yang, X.-C., C. Labarca, J. Nargeot, B.Y. Ho, O. Elroy-Stein, B. Moss, N. Davidson, H.A.
Lester. (1992) Cell specific posttranslational events affect functional expression at the
plasma membrane but not tetrodotoxin sensitivity of rat brain IIA sodium channel alpha
subunit expressed in mammalian cells. J. Neuroscience 12, 268-277.
15. Morgan,R.A., L. Couture, O. Elroy-Stein, J. Ragheb, B. Moss, W.F. Anderson. (1992)
Retroviral vectors containing putative internal ribosome entry sites: development of a
polycistronic gene transfer system and application to human gene therapy. Nucleic Acids
Research 20, 1293-1299.
16. Davies, M.V., O. Elroy-Stein, R, Jagus, B. Moss, R.J. Kaufman. (1992) The vaccinia K3L
gene product potentiates translation by inhibiting double- stranded-RNA-activated protein
kinase and phosphorylation of the alpha subunit of eukaryotic initiation factor 2. J. Virol.
66, 1943-1950.
17. Zibert, A., H.C. Selinka, O. Elroy-Stein, E. Wimmer. (1992) The soluble form of two Nterminal domains of the poliovirus receptor is sufficient for blocking viral infection. Virus
Research 25, 51-61.
18. Carroll, K., O. Elroy-Stein, B. Moss, R. Jagus. (1993) Recombinant vaccinia virus K3L gene
product prevents activation of dsRNA-dependent, eIF-2a specific protein kinase. J. Biol.
Chem. 268, 12837-12842.
19. Whetter, L.E., S.P. Day, O. Elroy-Stein, E.A. Brown, S.M. Lemon. (1994) Low efficiency of
the 5' Nontranslated Region of Hepatitis A RNA is Promoting Cap-Independent Translation
in Permissive Monkey Kidney Cells. J. Virol. 68, 5253-5263.
20. Whetter, L.E., S.P. Day, E.A. Brown, O. Elroy-Stein, S.M. Lemon. (1994) Analysis of
hepatitis A virus translation in a T7 polymerase-expressing cell line. Arch. Virol. 9, 291298.
21. Zrihan-Licht, S., H.L. Vos, A. Baruch, O. Elroy-Stein, D. Sagiv, I. Keydar, J. Hilkens, D.H.
Wreschner. (1994) Characterization and molecular cloning of a novel MUC1 protein devoid
of tandem repeats expressed in human breast cancer tissue. Eur. J. Biochem. 224, 787-795.
22. Zrihan-Licht, S., A. Baruch, O. Elroy-Stein, I. Keydar, D.H. Wreschner. (1994) Tyrosine
phosphorylation of the MUC1 breast cancer membrane proteins: Cytokine receptor-like
molecules. FEBS Lett. 356, 130-136.
23. Bernstein, J., I. Sheffler, O. Elroy-Stein. (1995) The translational repression mediated by the
PDGF/c-sis mRNA leader is relieved during megakaryocytic differentiation. J. Biol. Chem.
270, 10559-10565.
24. Shterman, N., O. Elroy-Stein, I. Morad, M. Amitsur, G. Kaufmann. Cleavage of the HIV
replication primer tRNA lys,3 in human cells expressing bacterial anticodon nuclease.
(1995) Nucleic Acids Res. 23, 1744-1749.
25. Pasmanik-Chor, M., O. Elroy-Stein, S. Gatt, H. Aerts, M. Horowitz. (1996) Overexpression of human glucocerebrosidase containing different size leaders. Biochem. J. Vol.
316, 81-88.
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Orna Elroy-Stein, Ph.D.
Tel Aviv University
26. Pasmanik-Chor, M., S. Laadan, O. Elroy-Stein, A. Zimran, A. Abrahamov, S. Gatt, M.
Horowitz. (1996) The glucocerebrosidase D409H mutation in Gaucher disease. Biochem. &
Molec. Med. 59, 125-133.
27. Strauss, D., O. Elroy-Stein, R. Ehrlich. (1997) Adenovirus E1A interferes with expression of
vaccinia viral genes. Gene. 184, 279-284.
28. Bernstein, J., O. Sella, S-Y. Le, O. Elroy-Stein. (1997) PDFG2/c-sis mRNA leader contains
a differentiation-induced internal ribosomal entry site (D-IRES). J. Biol. Chem. 272, 93569362.
29. Savitsky, K., M. Platzer, T. Usiel, S. Gilad, A. Sartiel, A. Rosenthal, O. Elroy-Stein, Y.
Shiloh, G. Rotman. (1997) Ataxia-telangiectasia: Structural diversity of untranslated
sequences suggests complex post-transcriptional regulation of ATM gene expression.
Nucleic Acid Res. 25, 1678-1684.
30. Pasmanik-Chor, M., L. Madar-Shapiro, O. Elroy-Stein, H. Aerts, S. Gatt, M. Horowitz.
(1997) Expression of human mutated glucocerebrosidase alleles in human cells. Hum. Mol.
Genet. 6, 887-895.
31. Gerlitz, G. and O. Elroy-Stein. (1997) Does EBV modulates ADA mRNA translation?
Leukemia 11, 249.
32. Akiri, G., D. Nahari, Y. Finkelstein, S.-Y. Le, O. Elroy-Stein, B.-Z. Levi. (1998) Regulation
of vascular endothelial growth factor (VEGF) expression is mediated by internal initiation
of translation and alternative initiation of transcription. Oncogene 17, 227-237.
33. Sella, O., S-Y. Le, G. Gerlitz, O. Elroy-Stein. (1999) Differentiation-induced internal
translation of PDGF2/c-cis mRNA: analysis of the cis-elements and their differentiationlinked binding to hnRNP-C. Molec. Cell Biol. 19, 5429-5440.
34. Finkelstein, Y., O. Factor, O. Elroy-Stein, B.Z. Levi. (1999) The use of bi-cistronic transfer
vectors for the baculovirus expression sytem. J. Biotechnol. 75, 33-44
35. Pozner, A., V. Negreanu, D. Goldenberg, S.-Y. Le, O. Elroy-Stein, D. Levanon, Y. Groner.
(2000) Transcription Coupled-Translation Control of AML1/RUNX1 is Mediated by Capand IRES-dependent Mechanisms. Molec. Cell. Biol. 20, 2297-2307
36. Gerlitz, G., R. Jagus, O. Elroy-Stein. (2002) Phosphorylation of initiation factor 2 is
required for activation of internal translation initiation during cell differentiation. Eur. J.
Biochem. 269, 2810-2819.
37. Koloteva-Levine, N., M. Amichay, O. Elroy-Stein. (2002) Interaction of hnRNP-C1/C2
proteins with RNA: analysis using the yeast three-hybrid system. FEBS Letters 523, 73-78;
38. Koloteva-Levine, N., D. Pinchasi, I. Pereman, A. Zur, M. Brandeis, O. Elroy-Stein. (2004)
The Apc5 subunit of APC/Cyclosome interacts with PABP and represses IRES-mediated
translation. Molec. Cell. Biol. 24, 3577-3587
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Orna Elroy-Stein, Ph.D.
Tel Aviv University
39. Dahary, D., O. Elroy-Stein, R. Sorek (2005) Naturally occurring antisense: Transcriptional
leakage or real overlap? Genome Research 15, 364-368
40. Kantor, L., H. P. Harding, D. Ron, R. Schiffmann, C. R. Kaneski, S. Kimball, O. ElroyStein. (2005) Heightened stress response in primary fibroblasts expressing mutant eIF2B
genes from CACH/VWM leukodystrophy patients. Hum. Genet. 118, 99-106
41. Schiffmann, R. and O. Elroy-Stein. (2006) Childhood Ataxia with CNS
Hypomyelination/Vanishing White Matter Disease - A Common Leukodystrophy Caused by
Abnormal Control of Protein Synthesis. Mol Genet Metab. 88, 7-15
42. Silvera, D., N. Koloteva-Levine, S. Burma, O. Elroy-Stein. (2006) Effect of Ku proteins on
IRES-mediated translation. Biol Cell 98, 353-361
43. Sivan G., N. Kedersha, O. Elroy-Stein (2007) Ribosomal slowdown mediates translational
arrest during cellular division. Mol Cell Biol. 27, 6639-6646
44. Sivan G. and O. Elroy-Stein (2008) Translational control during cellular division. Cell
cycle. Vol. 7, 741-744
45. Marash L., N. Liberman, S. Henis-Korenblit, G. Sivan, E. Reem, O. Elroy-Stein, A. Kimchi
(2008) DAP5 promotes cap-independent translation of Bcl-2 and CDK1 to facilitate cell
survival during mitosis. Mol Cell. 30, 447-459
46. Kantor L, D. Pinchasi, M. Mintz, Y. Hathout, A. Vanderver, O. Elroy-Stein (2008) A Point
mutation in Translation Initiation Factor 2B Leads to a Continuous Hyper Stress State in
Oligodendroglial-Derived Cells. PLoS ONE. 3(11):e3783.
47. Marom L., S. Hen, D. Pinchasi, J.A. Chekanova, D.A. Belostotsky, O. Elroy-Stein (2009).
Diverse poly(A) binding proteins mediate internal translational initiation by a plant viral
IRES. RNA Biol. 6(4), 446-454
48. Raveh-Amit H., A. Maissel, J. Poller, L. Marom, O. Elroy-Stein, M. Shapira, and E. Livneh
(2009). Translational Control of Protein Kinase C by Two Upstream Open Reading
Frames (uORFs). Mol Cell Biol. 29(22), 6140-6148
49. Geva M., Y. Cabilly, Y. Assaf, N. Mindroul, L. Marom, G. Raini, D. Pinchasi and O. ElroyStein. (2010). A mouse model for eIF2B-leukodystrophy reveals abnormal development of
brain white matter. Brain. 133, 2448-2461
50. Horzinski, L., L. Kantor, A. Huyghe, R. Schiffmann, O. Elroy-Stein, O. Boespflug-Tanguy
and A. Fogli (2010). Evaluation of the endoplasmic reticulum-stress response in eIF2Bmutated lymphocytes and lymphoblasts from CACH/VWM patients. BMC Neurology. 10,
94-101
51. Mor, E., Y. Cabilly, Y. Goldshmit, H. Zalts, S. Modai, L. Edry, O. Elroy-Stein, and N.
Shomron (2011) Species-specific microRNA roles elucidated following astrocyte activation.
Nucleic Acids Res. 39, 3710-3723.
52. Sivan G., Aviner R., and O. Elroy-Stein (2011) Mitotic modulation of translation elongation
factor 1 leads to hindered tRNA delivery to ribosomes. J. Biol. Chem. 286 (32), 279277935
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Orna Elroy-Stein, Ph.D.
Tel Aviv University
53. Barhoom, S., J. Kaur, B. S. Cooperman, N. I. Smorodinsky, Z. Smilansky, M. Ehrlich, and
O. Elroy-Stein (2011) Quantitative single cell monitoring of protein synthesis at subcellular
resolution using fluorescently labeled tRNA. Nucleic Acids Res. 39:e129, July 27, 1-13
54. Marom, L., Ulitsky I., Cabilly Y., Shamir, R., and O. Elroy-Stein (2011) A point mutation in
translation initiation factor eIF2B leads to function- and time- specific changes in brain gene
expression. PLoS One, 3(11): e3783
55. Cabilly Y., Brabi M., Geva M., Marom L., Chetrit D, Ehrlich M., and O. Elroy-Stein (2012)
Poor inflammatory response in eIF2B knock-in mice: Implications for the etiology of
Vanishing White Matter diseas. PloS One 7(10):e46715
56. Aviner R., Geiger T., and O. Elroy-Stein (2013) Novel Proteomic Approach (PUNCH-P) Reveals
Cell Cycle Specific Fluctuations in mRNA Translation. Genes Dev. 27, 1834-1844
Highlighted in:
Nature Chemical Biology
Miura, G. "Pack a PUNCH". Research Highlights, Nature Chemical Biology 9,
601 (2013) doi:10.1038/nchembio.1347
Published online 17 September 2013
SciBX: Science-Buisness eXchange (Nature.com Publication)
Lou, K-J. "Puromycin-associated nascent chain proteomics (PUNCH-P) for
monitoring protein synthesis" SciBX 6(35); doi:10.1038/scibx.2013.971
Published online 12 September 2013
57. Barhoom S., Farrell I., Shai B., Dahari D., Cooperman B.S., Smilansky Z., Elroy-Stein O., and M.
Ehrlich. (2013) Dicodon monitoring of protein synthesis (DiCoMPS) reveals levels of synthesis
of a viral protein in single cells. Nucleic Acids Res. Aug 21, 1-10. doi: 10.1093/nar/gkt686
58. Aviner R., Geiger T., and O. Elroy-Stein. (2014) Genome-wise identification and quantification of
protein synthesis in cultured cells and whole tissues by puromycin-associated nascent chain
proteomics (PUNCH-P). Nature Protocols 9(4), 751-760
59. Aviner R., Geiger T., and Elroy-Stein O. (2014) PUNCH-P for global translatome profiling:
Methodology, insights and comparison to other techniques. Translation 1(1), eLocation ID:
e27516 http://dx.doi.org/10.4161/trla.27516
CHAPTERS IN BOOKS
1. Leiman-Hurwitz, J., N. Dafni, L. Sherman, D. Levanon, Y. Bernstein, E. Danciger, O.
Elroy-Stein, Y. Groner. Molecular structure and expression of the gene locus on
chromosome 21 encoding the Cu/Zn-superoxide dismutase and its relevance to Down's
syndrome. New York, Ann. New York Acad. Sci. Vol.450, 1985 (pp.133-156).
2. Groner, Y., N. Dafni, L. Sherman, D. Levanon, Y. Bernstein, E. Danciger, O. Elroy-Stein,
A. Neer. Down syndrome and Alzheimer's disease: Are common genes from human
chromosome 21 involved in both disorders? In: A. Fisher, I. Hanin and C. Lachman (eds.)
Alzheimer's and Parkinson's Disease, New York, Plenum Publish. Co., 1986 (pp. 271-285).
5
Orna Elroy-Stein, Ph.D.
Tel Aviv University
3. Groner, Y., L. Leiman-Hurwitz, N. Dafni, Y. Bernstein, L. Sherman, D. Levanon, E.
Danciger, O. Elroy-Stein. The human CuZnSOD family: Architecture and expression of the
chromosom 21- encoded functional gene and its related processed pseudogenes. In: G.
Rotilio (ed.) Superoxide and superoxide dismutase in Chemistry, Biology and Medicine.
New York, Elsevier Sci. Publish. 1986 (pp. 257-265).
4. Elroy-Stein, O., Y. Bernstein, K.B. Avraham, N. Dafni, D. Levanon, E. Danciger, A. Neer,
Y. Groner. Overexpression of the human Cu/Zn- superoxide dismutase gene in transfected
cells - Implication to Down's syndrome. In: A. Shahar et al. (eds.) Model systems in
Neurotoxicology - Alternative Approaches to Animal Testing. Progress in Clinical and
Biological Research. New York, Alan R. Liss, Inc., Vol.253, 1987 (pp.373-392).
5. Fuerst, T.R., O. Elroy-Stein, B. Moss. Translation enhancement of mRNA conferred by
Encephalomyocarditis virus 5' sequence improves the performance of the Vaccinia virus\
Bacteriophage T7 hybrid expression system. In: F. Brown, R.M. Chanock, H.S. Ginsberg
and R.A. Lerner (eds.) Modern Approaches to New Vaccines Including Prevention of AIDS.
Vaccines 90. Cold Spring Harbor, Cold Spring Harbor Laboratory Press, 1990 (pp.151-155)
6. Elroy-Stein O. and B. Moss. Gene Expression using the Vaccinia virus/T7 RNA Polymerase
Hybrid System. In: F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A.
Smith, and K. Struhlm (eds.) Current Protocols in Molecular Biology. New York, John
Wiley & Sons, Inc. Vol.2, 1992, Unit 16.19
7. Elroy-Stein O. and W. C. Merrick. (2007) Translation initiation by cellular ribosomal entry
sites. In: J.W.B. Hershey, M.B. Mathews, N. Sonenberg (eds.) Translational Control in
Biology and Medicine. Cold Spring Harbor Laboratory Press, New York.
8. Elroy-Stein O. and R. Schiffmann. (2015) Vanishing White Matter Disease. In: J.M.
Pascual and R.N. Rosenberg (eds.) ‘Rosenberg’s Molecular and Genetic Basis of
Neurological and Psychiatric Disease, Fifth Edition’ Elsevier Academic Press, San Diego
PATENTS
Moss Bernard, Fuerst Thomas,Elroy-Stein Orna.
Rapid, versatile and simple system for expressing genes in eukaryotic cells.
Patent number: 5135855, Issued on 04/08/1992, USA
Moss Bernard, Elroy-Stein Orna
Stable mammalian cell line expressing a bacteriophage RNA polymerase.
Patent number: 5126251, Issued on 30/06/1992, USA
Barhoom Sima, Marcelo Ehrlich, Elroy-Stein Orna
Detection of Cellular Stress by Live Imaging of tRNA Localization
Ramot ref. 2010022-00-00; Filed on 22/7/2010
6