MARINE BIOLOGICAL LABORATORY AQUATIC RESOURCES DIVISION WOODS HOLE, MASSACHUSETTS 02543-1015 E-mail: specimens@mbl.edu Telephone: (508) 289-7375 Fax: (508) 289-7900 MARINE ORGANISMS FOR AQUARIA AND THEIR REQUIRED MAINTENANCE The Marine Biological Laboratory's Aquatic Resources Division offers a selection of marine organisms. This collection of live marine organisms is an economical way for students to observe a mini-intertidal ecosystem of invertebrate life in your classroom. This Collection Includes the Following: 2 Brittle Starfish (Ophioderma brevispina) 3 Periwinkle Snails (Littorina littorea) 2 Starfish (Asterias forbesi) 3 Hermit Crabs (Pagurus longicarpus) 2 Sea Urchins (Arbacia punctulata) 2 Sea Cucumbers (Sclerodactyla briareus) 4 Grass Shrimp (Palaemonetes sp.) 2 Barnacles (Balanus sp.) 2 Small Blue Mussels (Mytilus edulis) 3 Mud Snails (Nassarius o) Small amount of Sea Lettuce (Ulva) 10 gallons of Woods Hole Natural Filtered Sea Water A few of these organisms like snails, mussels, and barnacles are not very hardy and/or may be preyed upon by other organisms in this set. They are included for the observational opportunity they provide. With reasonable care, most of the organisms will live for an extended period of time. ORGANISM CARE AND FEEDING: Check the aquarium daily upon arrival in the classroom and remove any dead organisms. Before leaving for the day, check the aquarium again. Remove all except two pieces of uneaten food and any dead organisms. Make sure the air pump is operating properly. Individually feeding the aquarium organisms each weekday with a 1/8” x 1/8” piece of food is good. Organisms may be fed chopped 1/8" pieces of fresh clams, raw shrimp, or fresh fish. Include a small amount of the liquid produced when preparing the food. One small cherry stone clam, blue mussel, or small uncooked shrimp will be enough food for several servings. Buy these fresh or fresh frozen. Beware of using commercially frozen sea food as it is likely to contain preservatives that could adversely affect the organisms. Note the total amount of food eaten per feeding. Two hours after feeding remove any uneaten food, except for two pieces. DO NOT CONTAMINATE THE AQUARIUM SEAWATER BY OVERFEEDING. (Cloudy, foul-smelling seawater is an indication of overfeeding, or a missed dead animal.) Process the remaining amount of fresh food into serving portions. Individually package the serving portions along with any liquid, and freeze. On feeding days, thaw the food and feed it to the animals in the aquarium. Holding bits of food (e.g., clam) next to the starfish and urchins will enable them to grasp the food with their tube feet before the hermit crabs or grass shrimp take it away. If the starfish and/or urchins are attached to the glass, you will be able to watch them move the food to their mouths, which are located on the undersides of their bodies. Brittle stars have long, thin arms and move rapidly along the bottom of the tank, searching for the food. Coral, barnacles, and sea cucumbers will feed on live Artemia (i.e., brine shrimp, sometimes called "sea monkeys"). Live Artemia or their eggs are available at most aquarium supply stores. By following instructions enclosed with the eggs, you can easily hatch them in a separate container. The coral may be fed, if the tentacles are extended, by allowing a tiny piece of food to gently land on their mouths (extended tentacles circle the mouth). We also recommend liquid invertebrate food (or algae cultures) for the coral, sea cucumbers, mussels and barnacles. Liquid invertebrate food may be purchased at your local pet store, by catalog, or online. If your sea lettuce expires we recommend putting small pieces of dried Nori seaweed into the aquarium daily. Remove any left over Nori the next day. Nori can be purchased at most supermarkets or health food stores. I hope that you and your students enjoy observing these interesting ocean organisms. I welcome questions, comments, or suggestions from you. Sincerely, Edward G. Enos, Jr. Superintendent, Aquatic Resources Division Marine Biological Laboratory 1. Was this set of instructions helpful? 2. How can we improve them? 3. How are you using these creatures in your classroom? 4. Would you mind if we listed your classroom methods and acknowledged you? Recommended References: Blasiola, George. The New Saltwater Aquarium Handbook. Barrons Educational Series, Inc., Hauppauge, NY, 1991 (ISBN: 0812044827). Coulombe, Deborah A. The Seaside Naturalist: A Guide to Study at the Seashore. Prentice Hall Press, New York, NY, 1992 (ISBN: 0671765035). Gosner, Kenneth L. and Peterson, Roger T. .A Field Guide to the Atlantic Seashore (Peterson Field Guides). Houghton Mifflin Co., Boston, MA, 1999 (ISBN: 061800209X). 01/03