Hellenic Accreditation System S.A. Annex F1/3 to the Certificate No. 571-2 SCOPE of ACCREDITATION of the Laboratory of Plant Biotechnology & GMO Testing of the Mediterranean Agronomic Institute of Chania (MAICh) Materials / Products tested Types of test / Properties measured Applied standards / Techniques used Biological Tests 1. Wheat: seeds, plant tissue, foodstuffs (grains, flour) 1. Screening method for genetically modified organisms (GMOs), by the detection of CaMV 35S promoter and nopaline synthase terminator (NOS). In house method (O610) based on ISO 21569:2005 and the scientific papers: Iida M. et al, J. of Agricultural and Food Chemistry 53(16):6294-6300, 2005, and Taberlet P. et al, Plant Molecular Biology 17: 1105-1109, 1991, Lipp M. et al, Eur. Food Res. Technol., 212: 497-504, 2001, using PCR and electrophoresis. DNA extraction with the DNAExtractor kit, Eurofins Genescan. 2. Tomato: seeds, plant tissue, foodstuffs (fruit, juice) 1. Screening method for genetically modified organisms (GMOs), by the detection of CaMV 35S promoter and nopaline synthase terminator (NOS). In house method (O620) based on ISO 21569:2005 and the scientific papers: Yang L. et al., J. of Agricultural and Food Chemistry, 53(2):183-90, 2005, and Lipp M. et al, Eur. Food Res. Technol., 212: 497-504, 2001, using PCR and electrophoresis. DNA extraction with the DNAExtractor kit, Eurofins. 3. Soya: seeds, foodstuffs (flour), feedstuffs 1. Screening method for genetically modified organisms (GMOs), by the detection of CaMV 35S promoter and nopaline synthase terminator (NOS). In house method (O650) based on ISO 21569:2005 and the scientific papers: Meyer R. et al., Z. Lebensm. Unters. Forsch., 203, pp 339-344, 1996, and Lipp M. et al, Eur. Food Res. Technol., 212: 497-504, 2001, using PCR and electrophoresis. DNA extraction with the DNAExtractor kit, Eurofins. Page 1 of 2 Annex F1/3 to the Certificate No. 571-2 21.06.2013 Materials / Products tested 4. Maize: seeds, foodstuffs (grains, flour), feedstuffs Types of test / Properties measured 1. Screening method for genetically modified organisms (GMOs), by the detection of CaMV 35S promoter and nopaline synthase terminator (NOS). Applied standards / Techniques used In house method (O660) based on ISO 21569:2005 and the scientific papers: Sachs M.M. et al., Genetics 113 (2), 449467, 1986, and Lipp M. et al, Eur. Food Res. Technol., 212: 497-504, 2001, using PCR and electrophoresis. DNA extraction with the DNAExtractor kit, Eurofins. Site of assessment: Permanent Laboratory premises, 1 Macedonias str., Alsyllio Agrokipiou, P.O. Box 85, GR-73 100, Chania, Greece. Approved signatories: P. Kalaitzis, Th. Spanos. This scope of Accreditation replaces the previous one dated 29.07.2010. The Accreditation Certificate No. 571-2, to ELOT EN ISO/IEC 17025:2005, is valid until 24.06.2017. Athens, June 21, 2013 Ioannis Sitaras Director of the Laboratories Accreditation Division Page 2 of 2 Annex F1/3 to the Certificate No. 571-2 21.06.2013