Pathology Services: A Practical Handbook (5 th Edition, 2008)
Summary of Updates
Updated (S/N 1 – 71) and Uploaded: Apr 2010
S/N
Section #
1
INTRODUCTION
Title
COMMITTEE 2007 - 2010
Page/s
5
Update
Under MEMBERS
Effective
Date
1 Mar 10
Amend:
Dr Tien Sim Leng
To:
A/Prof Tien Sim Leng
2
1
THE ORGANISATION
11
Under LOCATION OF LABORATORIES
Under Cytogenetics Lab
1 Mar 10
After Tel No. 63214678/50
Insert: Tel No. 63213632
3
1
THE ORGANISATION
12
Under PERSONNEL
Under Professional Staff
1 Mar 10
CYTOGENETICS LAB
After Lim Tse Hui
Insert: Scientific Officer
Karen See Hsu-Shien
BSc (Hons)
1
4
1
THE ORGANISATION
14
Under PERSONNEL
Under PROFESSIONAL STAFF
1 May 08
MOLECULAR LAB
Amend:
Head & Consultant
Dr Lynette Oon Lin Ean
BSc (Hons), MBBS, MRCP, FRCPA
Senior Scientific Officer
Dr Eileen Chen Xueqin
B.Med, PhD
Scientific Officer
Ms Lim Kun Lee
BSc (Hons), MSc
To
Head & Senior Consultant
Dr Lynette Oon Lin Ean
BSc (Hons), MBBS, MRCP, FRCPA
Principal Scientific Officer
Dr Eileen Chen Xueqin
B.Med, PhD
Senior Scientific Officer
Ms Lim Kun Lee
BSc (Hons), MSc
2
5
2
LABORATORY POLICIES &
SERVICE INFORMATION
23
Under EXTERNAL QUALITY ASSESSMENT PROGRAMMES
1 Jan 2010
Under Molecular
Delete :
QCMD Toxoplasma gondii
6
2
LABORATORY POLICIES &
SERVICE INFORMATION
25
Amend :
CAP CMV and EBV Viral Load
2/year
To
CAP BKV, CMV and EBV Viral Load
2/year
Insert:
CAP HLA-B27 Typing Survey
CAP EGFR
CAP KRAS
QCMD (JC virus and BK virus)
QCMD (B19 virus DNA)
QCMD (CMV DNA)
QCMD (HHV6 DNA)
QCMD (EBV DNA)
QCMD (Clostridium difficile DNA)
2/year
2/year
2/year
1/year
1/year
1/year
1/year
1/year
1/year
Under OPERATING HOURS
1 Apr 2010
Under OFFICE HOURS
Delete
Monday to Friday
Saturday
8.30am - 5.30pm (Molecular Lab)
8.30am – 1.00pm (Molecular Lab)
(The Molecular Laboratory will be following the same opening hours of the
Department.)
3
7
4
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
Biochemistry
70
Under CALL-BACK VALUES OF CRITICAL ANALYTES
18 Feb 10
Amend:
Potassium
Sodium
Calcium
Glucose
pH (blood gas)
Blood Ketone
Digoxin
Theophylline
Paracetamol
Salicylate
Lithium
<2.5
or
<120 or
<1.75 or
<2.0
or
<7.1
or
>1.4
>2.5
>25.0
all results
all results
2.0
>5.7
>160
>3.25
>25.0
>7.6
To
Potassium
Sodium
Calcium
Glucose
pH (blood gas)
Calcium,Ionic
Blood Ketone
Digoxin
Theophylline
Paracetamol
Salicylate
Lithium
<2.5
or
<120 or
<1.75 or
<2.5
or
<7.1
or
<0.80 or
>1.4
>2.5
>25.0
all results
all results
>2.0
>5.7
>160
>3.25
>25.0
>7.6
>1.60
mmol/L
mmol/L
mmol/L
mmol/L
mmol/L
mg/L
mg/L
mg/L
mmol/L
mmol/L
mmol/L
mmol/L
mmol/L
mmol/L
mmol/L
mmol/L
mg/L
mg/L
mg/L
mmol/L
mmol/L
4
8
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
Biochemistry
72
Under ALPHAFOETO PROTEIN (AFP) FRACTION 3
01 Jan 10
Amend:
Reference interval
:
> 12 years: 0 – 14.9%
:
> 12 years: <10%
To
Reference interval
9
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
Biochemistry
85
Under EVEROLIMUS, BLOOD
02 Nov 09
Amend:
Method
:
Immunoassay
To
Method
10
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
Biochemistry
88
:
Liquid Chromatography/Tandem Mass
Spectrometry
Under HOMOCYSTEINE,PLASMA
16 Mar 10
Amend:
Method
:
Colorimetry
:
Immunoassay
To
Method
5
11
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
Biochemistry
94
Under METANEPHRINE,URINE
25 Feb 10
Amend:
Reference Interval
:
:
3 – 8 years: 26-137 nmol/mmol creatinine
9 – 19 years: 18-126 nmol/mmol creatinine
:
:
3 – 8 years: 27-138 nmol/mmol creatinine
9 – 19 years: 19-126 nmol/mmol creatinine
To
Reference Interval
12
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
Biochemistry
98
Under NORMETANEPHRINE,URINE
25 Feb 10
Amend:
Reference Interval
:
:
3 – 8 years: 38-434 nmol/mmol creatinine
9 – 19 years: 28-359 nmol/mmol creatinine
:
:
3 – 8 years: 38-435 nmol/mmol creatinine
9 – 19 years: 29-360 nmol/mmol creatinine
To
Reference Interval
6
13
4
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
Cytogenetics
122
After ‘CHROMOSOME ANALYSIS OF SOLID TUMOURS’
1 Mar 10
[effect is to move the entry from pg 130-131 to pg122]
Insert: RAPID PRENATAL ANEUPLOIDY SCREENING, DOWN SYNDROME
SCREENING AND SEX CHROMOSOME DETERMINATION WITH
FLUORESCENCE IN SITU HYBRIDIZATION TEST
Common indications : Advanced maternal age (≥ 35 years at EDD), Abnormal
ultrasound findings, Positive maternal serum screening,
Previous history of chromosome abnormality, Family history of
Down syndrome, Parental anxiety
Special instructions : This FISH test is usually requested in conjunction with
conventional cytogenetic studies.
Sample required :
Amniotic fluid (3 mL), chorionic villi (5 mg), or fetal cord
blood/neonate blood (0.5 mL)
Method :
Chromosome analysis as with Amniotic Fluid/Chorionic Villi or
Fetal Cord Blood/Neonate Blood
FISH assay using LSI 13 and 21, and CEP X, Y, and 18 DNA
probes labelled with fluorophores are analysed under
fluorescence microscopy.
Test results :
Normal or Abnormal, with the abnormalities listed.
Findings are reported in accordance to the
InternationalSystem for Human Cytogenetic Nomenclature
(ISCN, 2009).
Reference values :
Individuals with a trisomy will show three signals of a particular
DNA probe. Normal individuals will show two signals of each
DNA probe. Females will show two signals of the X probe,
males one signal of the X and Y probe each.
Turnaround time :
Within 3 days
Day(s) test set up :
Monday – Friday (office hours)
7
14
4
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
Cytogenetics
122
After RAPID PRENATAL ANEUPLOIDY SCREENING, DOWN SYNDROME
SCREENING AND SEX CHROMOSOME DETERMINATION WITH
FLUORESCENCE IN SITU HYBRIDIZATION TEST
1 Mar 10
Insert : CHROMOSOME ANALYSIS WITH FLUORESCENCE IN SITU
HYBRIDIZATION (FISH)TEST FOR MICRODELETION SYNDROMES
Common Indications:
This test is useful for patients suspected of PraderWilli (PW) / Angelman (AS), Di-George (DG) or
Williams (WS) syndromes. Other microdeletion
probes available include the SRY gene probe.
Special Instructions:
This FISH test is always used in conjunction with
conventional cytogenetic studies. Contact the
Cytogenetics laboratory before sending a specimen.
Sample Required:
Peripheral blood
Method:
Long-term culture to obtain metaphases. Probes used
for metaphase and interphase FISH (PWS/AS, DGS
or WS region DNA probes) are labeled with
fluorophores and analysed under fluorescence
microscopy.
Test Results:
Deleted or Not deleted. Nomenclature given is in
accordance to the International System for Human
Cytogenetic Nomenclature (ISCN, 2009).
Reference Values:
Individuals with a microdeletion will show two copies
of the internal control signals but only one copy of the
locus-specific signal for that region of interest.
Turnaround Time:
Within 1 week
Day(s) Test Set Up:
Monday – Saturday (office hours)
8
15
4
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
Cytogenetics
123 -127 Delete Tests:
1Mar 10
INTERPHASE FLUORESCENCE IN SITU HYBRIDIZATION TEST WITH DUAL
FUSION TRANSLOCATION PROBES - BCR/ABL1
INTERPHASE FLUORESCENCE IN SITU HYBRIDIZATION (FISH) TEST WITH
DUAL FUSION TRANSLOCATION PROBES – ETO/AML1
INTERPHASE FLUORESCENCE IN SITU HYBRIDIZATION (FISH) TESTS WITH
DUALCOLOUR BREAKAPART PROBES – CBFB (INV 16), MLL (11Q23), RARA
(17Q21) IN ACUTE MYELOID LEUKAEMIA
INTERPHASE FLUORESCENCE IN SITU HYBRIDIZATION (FISH) TEST WITH
DUAL FUSION TRANSLOCATION PROBES – PML/RARA
INTERPHASE FLUORESCENCE IN SITU HYBRIDIZATION (FISH) PANEL TEST
FOR CHRONIC LYMPHOCYTIC LEUKAEMIA (CLL)
INTERPHASE FLUORESCENCE IN SITU HYBRIDIZATION (FISH) PANEL TEST
FOR MULTIPLE MYELOMA (MM)
INTERPHASE FLUORESCENCE IN SITU HYBRIDIZATION (FISH) PANEL TEST
FOR MYELODYSPLASTIC SYNDROME (MDS)
INTERPHASE FLUORESCENCE IN SITU HYBRIDIZATION TESTS FOR OTHER
HAEMATOLOGICAL MALIGNANCIES
INTERPHASE FLUORESCENCE IN SITU HYBRIDIZATION (FISH) TEST
FORANAPLASTIC LYMPHOMA KINASE (ALK)
9
16
4
SAMPLE COLLECTION &
123 - 127 After “CHROMOSOME ANALYSIS WITH FLUORESCENCE IN SITU
HANDLING – SPECIAL
HYBRIDIZATION (FISH)TEST FOR MICRODELETION SYNDROMES”
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
Insert: new FISH tests title “INTERPHASE FLUORESCENCE IN SITU
& TEST RESULTS:
HYBRIDIZATION (FISH) TEST FOR HAEMATOLOGICAL MALIGNANCIES”
Cytogenetics
Clinical Indications:
Refer to Table 1.
1. A Dual Colour Dual Fusion translocation (DF) probe
detects the juxtaposition of one gene locus with its
translocation gene partner. The translocation event
produces a fusion of the two genes on the derivative
chromosomes.
2.
A Dual Colour Breakapart (BA) probe detects
disruptions to gene sequences that are known to be
involved in translocations with another partner or
disruptions due to other rearrangements such as an
inversion.
3.
An Extra Signal Dual Colour translocation (ES) probe
detects the translocation between the genes of its
translocation partners.
4.
Single Colour Enumeration probes comprise locus
specific identifier probes or centromeric probes and
detect interstitial deletions or loss/gain of a whole
chromosome.
Special instructions:
Interphase FISH test may be requested in conjunction
with conventional cytogenetic studies or as a standalone
test depending on the disease status. The FISH assay
should preferably be done on diagnostic cases prior to
treatment so as to determine the baseline pattern.
Specimen required:
Blood or Bone marrow in sodium/lithium heparin
1 Mar 10
10
16
4
cont’d
123 - 127 Method:
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
Test results:
& TEST RESULTS:
Cytogenetics
Fluorescence In Situ Hybridization using direct-labelled
FISH DNA probes and analysed under fluorescence
microscopy
8
Normal or abnormal signal pattern depending on the
probe construction:
1.
Dual Fusion (DF) probe: Typical abnormal signal
pattern is 2 fusions, 1 red and 1 green pattern.
Variant pattern is outside the normal range.
2.
Breakapart (BA) probe: Typical abnormal signal
pattern is 1 fusion, 1 red and 1 green pattern.
Variant pattern is outside the normal range.
3.
Extra Signal (ES) probe: Typical abnormal signal
pattern is 1 fusion, 2 red signals and 1 green
pattern. Variant pattern is outside the normal
range.
4.
Enumeration probe: Loss or Gain of FISH signal.
FISH findings are reported in accordance to the
International System for Human Cytogenetic
Nomenclature (ISCN, 2009).
Reference values:
Please refer to Table 2 for the normal cut-off level for
each probe.
Turnaround Time:
Within 1 week
Day(s) test set up:
Monday – Saturday (office hours)
11
17
4
SAMPLE COLLECTION &
123 - 127 After “INTERPHASE FLUORESCENCE IN SITU HYBRIDIZATION (FISH) TEST
HANDLING – SPECIAL
FOR HAEMATOLOGICAL MALIGNANCIES”
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
Insert: new FISH tests title “INTERPHASE FLUORESCENCE IN SITU
& TEST RESULTS:
HYBRIDIZATION (FISH) PANEL TESTS FOR MULTIPLE MYELOMA,
Cytogenetics
MULTIPLE MYELOMA HYPERDIPLOIDY, B-CELL CHRONIC LYMPHOCYTIC
LEUKAEMIA (B-CLL) AND MYELODYSPLASTIC SYNDROME (MDS)”
Clinical Indications:
1 Mar 10
Many cytogenetic abnormalities are subtle or are found
in non-dividing cells that only interphase FISH can be
used to elucidate instead of conventional cytogenetics.
FISH panels offered by the laboratory include:
1.
2.
3.
4.
Multiple Myeloma: Expanded MM FISH panel can
be used to detect multiple chromosome aberrations
found in multiple myeloma.
Hyperdiploidy Multiple Myeloma: If hyperdiploidy is
suspected in multiple myeloma, MM hyperdiploidy
FISH panel can be offered.
B-cell Chronic Lymphocytic Leukaemia: CLL FISH
panel can be used to detect the multiple aberrations
that are often found in clonal B-cells.
Myelodysplastic Syndrome: MDS FISH panel are
used to detect MDS.
Special Instructions:
The FISH panel test is charged as separate test from the
accompanying cytogenetic test. Three to five DNA
probes are used in the panel test.
Specimen required:
Blood or bone marrow in sodium/lithium heparin. For Bcell CLL, the preferred specimen is peripheral blood.
Method:
Fluorescence In Situ Hybridization using direct-labelled
FISH DNA probes and analysed under fluorescence
microscopy. Refer to Table 3 for the probes used in each
panel.
12
17
4
cont’d
123 - 127 Test Results:
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
Reference Values:
& TEST RESULTS:
Cytogenetics
Turnaround Time:
Day(s) test set up:
18
4
SAMPLE COLLECTION &
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INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
Cytogenetics
128
Positive or Negative for the FISH panel. FISH findings
are reported in accordance to the International System
for Human Cytogenetic Nomenclature (ISCN, 2009).
Please refer to Table 4 for the normal cut-off level of
each probe.
Within 1 week
Monday – Saturday (office hours)
Under “INTERPHASE FLUORESCENCE IN SITU HYBRIDIZATION TEST FOR
BREAST CANCER TISSUES USING HER2 (ERBB2) DNA PROBE”
1 Mar 10
Under Special instructions :
Insert after “thickness”: on coated slides. The optimal fixation time in formalin
should be between 6-48 hours. An accompanying
Hematoxylin and Eosin stained slide with the tumour
region marked out should be submitted together with at
least 3 unstained sections.
Amend:
Reference values :
To:
Reference values :
Normal specimens have a HER2 (ERBB2) to CEP 17 signal
ratio of < 2.0. Specimens with amplification have a ratio of ≥
2.0….
Normal specimens have a HER2 (ERBB2) to CEP 17 signal
ratio of < 1.8. Specimens with amplification have a ratio of ≥
2.2….
Amend:
Turnaround time :
5 days (mean)
To:
Turnaround time :
Within 1 week
13
19
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SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
Cytogenetics
128-129 Delete Tests:
1Mar 10
INTERPHASE FLUORESCENCE IN SITU HYBRIDIZATION (FISH) TEST FOR
EWING SARCOMA
INTERPHASE FLUORESCENCE IN SITU HYBRIDIZATION (FISH) TESTS FOR
LYMPHOMA (PARAFFIN SECTIONS) – OTHER PROBES
INTERPHASE FLUORESCENCE IN SITU HYBRIDIZATION (FISH) TEST FOR
MUCOSAASSOCIATED LYMPHOID TISSUE (MALT) TYPE LYMPHOMA
14
20
4
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
Cytogenetics
129
After “INTERPHASE FLUORESCENCE IN SITU HYBRIDIZATION TEST FOR
BREAST CANCER TISSUES USING HER2 (ERBB2) DNA PROBE”
1 Mar 10
Insert: new FISH tests title “INTERPHASE FLUORESCENCE IN SITU
HYBRIDIZATION (FISH) TEST FOR LYMPHOMA AND SARCOMA ON
PARAFFIN-EMBEDDED SECTIONS OR TISSUE IMPRINTS’
Clinical Indications:
Refer to Table 1.
Lymphoma probes include ALK breakapart (BA), BCL6
(BA), c-MYC (BA), IGH/c-MYC (DF), API2/MALT1 (DF),
IGH/CCND1 (DF), IGH (BA), IGH/BCL2 (DF), MALT1
(BA).
Sarcoma probes include EWSR1 (BA) and SYT (BA).
Variant translocations can be detected using the
breakapart probe (BA).
Special Instructions:
The FISH test is optimal with fresh tissue samples.
Tissue sections should preferably be prepared between
4time in formalin should be between 6-48 hours. An
accompanying Hematoxylin and Eosin stained slide with
the tumour region marked out should be submitted
together with at least 3 unstained sections.
Specimen required:
Freshly-cut tissue sections or tissue imprints. Bone
marrow aspirates can only be used if there is
lymphomatous involvement.
Method:
Fluorescence In Situ Hybridization using direct-labelled
FISH DNA probes and analysed under fluorescence
microscopy.
Test results:
Normal or abnormal signal pattern depending on the
probe construction.
15
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INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
Cytogenetics
129
1. Dual Fusion (DF) probe: Typical abnormal signal
pattern is 2 fusions, 1 red and 1 green pattern.
Variant pattern is outside the normal range.
2. Breakapart (BA) probe: Typical abnormal signal
pattern is 1 fusion, 1 red and 1 green pattern. Variant
pattern is outside the normal range.
FISH findings are reported in accordance to the
International System for Human Cytogenetic
Nomenclature (ISCN, 2009).
Reference Values:
Please refer to Table 2 for the normal cut-off level of
each probe.
Turnaround Time:
Within 1 week
Day(s) test set up:
Monday – Saturday (office hours)
16
21
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HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
Cytogenetics
130
Under “INTERPHASE FLUORESCENCE IN SITU HYBRIDIZATION (FISH) PANEL
TEST FOR GLIOMA”
1 Mar 10
Under Special instructions
Insert after ‘thickness’: on coated slides. The optimal fixation time in formalin
should be between 6-48 hours. An accompanying
Hematoxylin and Eosin stained slide with the tumour
region marked out should be submitted together with at
least 3 unstained sections.
Amend:
Reference values :
To:
Reference values :
Losses of 1p36 and/or 19q13 regions equal to or greater
than 25% of interphase cells, and/or a ratio of 1p36/1q25
and/or 19q13/19p13 of equal to or less than 0.8.
Losses of 1p36 and/or 19q13 regions equal to or greater
than 17.2% of interphase cells, and/or a ratio of 1p36/1q25
and/or 19q13/19p13 of equal to or less than 0.8.
Amend:
Turnaround time :
5 days (mean)
To:
Turnaround time :
Within 1 week
17
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SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
Cytogenetics
After “INTERPHASE FLUORESCENCE IN SITU HYBRIDIZATION (FISH) PANEL
TEST FOR GLIOMA”
1 Mar 10
Insert: Table 1 - HAEMATOLOGY/ONCOLOGY FLUORESCENCE IN SITU
HYBRIDIZATION (FISH) AVAILABLE PROBES
FISH PROBES
ALK BA
1p36 / 19q13
BCL6 BA
IGH/FGFR3 DF
EGR1
EGFR (HER-1)
PROBE
LOCATION
2p23
1p36 / 19q13
3q27
4p16.3 / 14q32
5q31
7p12
D7S486
CEP 8
c-MYC BA
7q31
Cen 8
8q24
IGH/c-MYC DF
ETO/AML1 DF
BCR/ABL1 DF
CEP 9, 11, 15
8q24.1 / 14q32
8q22 / 21q22
9q34 / 22q11.2
Cen 9 / Cen 11/
Cen 15
11q23
11q22.3
11q21 / 18q21
11q13 / 14q32
MLL BA
ATM
API2/MALT1 DF
CCND1 XT/IGH
DF
CLINICAL INDICATIONS
Anaplastic Large Cell Lymphoma
Gliomas
DLCL, FL
Multiple Myeloma
MDS, AML
Solid tumours: head and neck, ovarian,
cervical, bladder, oesophageal,
non-small cell lung cancer, breast
MDS, AML
AML, CML, MDS
Burkitt's Lymphoma (include variants),
ALL
Burkitt's Lymphoma, ALL
AML
ALL, AML, CML
Multiple Myeloma
ALL, AML
B-CLL
MALT lymphoma, DLBCL
Mantle Cell Lymphoma,
B-Prolymphocytic Leukaemia,
Plasma Cell Leukaemia,
Splenic Lymphoma, CLL,
Multiple Myeloma,
B-lineage lymphocyte
18
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SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
Cytogenetics
FISH PROBES
CEP 12
TEL/AML1 ES
RB1
PROBE
LOCATION
Cen 12
12p13 / 21q22
13q14
D13S25
IGH BA
IGH/BCL2 DF
13q14
14q32
14q32 / 18q21
PML/RARA DF
CBFB BA
TP53
15q22 / 17q21.1
16q22
17p13.1
HER-2/neu
(ERBB2)
RARA BA
MALT1 BA
SYT BA
D20S108
17q11.2q12
EWSRI BA
22q12
Probe
1.
2.
3.
17q21.1
18q21
18q11.2
20q12
CLINICAL INDICATIONS
B-CLL
B-ALL
Multiple Myeloma, CLL, AML, MDS,
CLD
B-CLL
Multiple Myeloma, ALL, CLL, NHL
DLCL, FL
AML
AML
Multiple Myeloma, B-CLL, ALL, AML,
MDS, NHL
Breast Cancer
AML-M3 (include variants)
MALT lymphoma, DLBCL
Synovial sarcoma
MDS, AML, polycythemia vera,
chronic neutrophilic leukemia
Ewing's sarcoma (include variants)
Constructions:
BA- Breakapart
DF- Dual Fusion
ES- Extra Signal
19
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HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
Cytogenetics
After Table 1,
1 Mar10
Insert: TABLE 2 - NORMAL CUT-OFF LEVEL FOR HAEMATOLOGICAL AND
ONCOLOGY FISH PROBES
PROBES
ALK
BCL6
EGFR (HER-1) / CEP 7
c-MYC
IGH/c-MYC/CEP 8
ETO/AML1
BCR/ABL1
MLL
ATM
API2/MALT1
TEL/AML1
IGH
IGH/BCL2
PML/RARA
CBFB
RARA
MALT1
SYT
EWSRI
CONSTRUCTION
BA
BA
Polysomy
BA
DF
DF
DF
BA
Loss
DF
ES
BA
DF
DF
BA
BA
BA
BA
BA
CUT-OFF LEVEL
%
3.77
3.08
21.65
1.48
1.48
1.48
1.48
2.34
7.39
1.48
1.48
5.67
1.48
1.48
2.34
3.08
2.33
5.66
7.39
20
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INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
Cytogenetics
After Table 2,
1 Mar 10
Insert: TABLE 3 - HAEMATOLOGICAL FLUORESCENCE IN SITU
HYBRIDIZATION (FISH) PANEL TESTS
FISH PANEL TESTS
Expanded MM Panel
FISH PROBES USED
IGH/FGFR3 DF
CCND1 XT/IGH DF
RB1
TP53
IGH/MAF DF
MM Hyperdiploidy Panel
CEP 9
CEP 11
CEP 15
CLL Panel
ATM
CEP 12
D13S25
TP53
MDS Panel
EGR1
D7S486
CEP 8
D20S108
21
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4
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
Cytogenetics
After Table 3,
1 Mar 10
Insert: TABLE 4 - NORMAL CUT-OFF LEVEL FOR FISH PANEL PROBES
EXPANDED MM PANEL
CONSTRUCTION
IGH/FGFR3
Cyclin D1 XT/IGH
RB1
TP53
IGH/MAF
DF
DF
Loss
Loss
DF
MM HYPERDIPLOIDY PANEL
CONSTRUCTION
CEP 9
CEP 11
CEP 15
Gain
Gain
Gain
CLL PANEL
CONSTRUCTION
ATM
CEP 12
D13S25
TP53
Loss
Gain
Loss
Loss
MDS PANEL
CONSTRUCTION
EGR1
D7S486
CEP 8
D20S108
Loss
Loss
Gain
Loss
CUT-OFF LEVEL
%
1.48
1.48
5.06
10.5
1.48
CUT-OFF LEVEL
%
2.33
1.48
3.08
CUT-OFF LEVEL
%
7.39
4.43
1.49
10.5
CUT-OFF LEVEL
%
7.39
8.48
3.77
8.48
22
26
27
28
4
4
4
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
MOLECULAR DIAGNOSTICS
185
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
MOLECULAR DIAGNOSTICS
185
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
MOLECULAR DIAGNOSTICS
Amend
ALPHABETICAL TEST LISTING – MOLECULAR LABORATORY
1 Jan 10
To
ALPHABETICAL TEST LISTING – INFECTIOUS DISEASES
186
Under BK VIRUS LOAD (QUANTITATIVE)
Amend:
Turnaround time
Day(s) test set up
: 2 – 14 days
: Monday
To
Turnaround time
Day(s) test set up
: 1 – 5 days
: Monday and Wednesday
Under CHLAMYDOPHILA (FORMERLY CHLAMYDIA) PNEUMONIAE &
MYCOPLASMA PNEUMONIAE PCR (MULTIPLEX PCR 1, QUALITATIVE)
Amend:
Turnaround time
Day(s) test set up
: 2 – 7 days
: Wednesday
To
Turnaround time
Day(s) test set up
: 1 – 4 days
: Variable
1 Jan 10
1 Jan 10
23
29
4
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
MOLECULAR DIAGNOSTICS
187
Before CYTOMEGALOVIRUS (CMV) PCR (QUALITATIVE)
Insert:
CYTOMEGALOVIRUS (CMV) LOAD (QUANTITATIVE)
Specimen required:
Storage and transportation:
Method:
Test results:
Turnaround Time:
Day(s) test set up:
30
4
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
MOLECULAR DIAGNOSTICS
15 Dec 09
187
3-5 ml of whole blood in EDTA
0.5-1 mL CSF / aqueous / vitreous fluid
Refrigerate sample until transfer to laboratory.
Do not freeze. Send sample at 2-8C. Sample
should reach the laboratory within 72 hours after
collection.
Real-time PCR (In-house)
Detected (quantity reported in copies/mL), Not
Detected or Inconclusive
Linear Range: 220 – 2,200,000,000 (2.349.34 log) copies/mL
1 - 5 days
Tuesday and Friday
Under CYTOMEGALOVIRUS (CMV) PCR (QUALITATIVE)
Amend:
Turnaround time
Day(s) test set up
: 1 – 7 days
: Wednesday
To
Turnaround time
Day(s) test set up
: 1 – 4 days
: Variable
1 Jan 10
24
31
4
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
MOLECULAR DIAGNOSTICS
188
Under HEPATITIS B VIRUS (HBV) LOAD (QUANTITATIVE)
Amend
Specimen required
To
Specimen required
Insert
Method
Amend
Test results
To
Test results
1 Jan 10
: 6 - 10 mL blood in sterile EDTA container.
Alternatively, at least 3 mL plasma (separated from
EDTA-treated blood within 1 day of collection by
centrifugation at 800-1600g for 20 minutes at room
temperature) in a sterile screw-capped polypropylene
tube.
: 3 - 5 mL blood in sterile EDTA container or serum
separation tube.
Alternatively, at least 1.5 mL plasma or serum
(separated within 1 day of collection by
centrifugation at 800-1600g for 20 minutes at room
temperature) in a sterile screw-capped polypropylene
tube.
: Real-Time PCR (Roche COBAS Ampliprep/Taqman
version 2.0)
: Quantity reported in copies/mL and/or IU/mL
Reported range : 12 IU/mL to 1.1E+08 IU/mL
Linear Range : 54.5 (Log 10 = 1.74) to at least
1.1E+08 (Log 10 = 8.03) IU/mL
: Quantity reported in copies/mL and/or IU/mL
Linear Range
: 20 to 1.7 X 10 8 IU/mL (116
to 9.9X10 8 copies/mL)
Lowest detection limit : 9 IU/mL
25
32
33
34
35
36
4
4
4
4
4
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
MOLECULAR DIAGNOSTICS
190
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
MOLECULAR DIAGNOSTICS
191
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
MOLECULAR DIAGNOSTICS
191
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
MOLECULAR DIAGNOSTICS
191
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
MOLECULAR DIAGNOSTICS
191
Under HEPATITIS C VIRUS PCR (QUALITATIVE)
Amend:
Turnaround time
Day(s) test set up
: 2 - 14 days
: Wednesday
To
Turnaround time
Day(s) test set up
: 2 – 10 days
: Variable
Under HERPES SIMPLEX VIRUS (HSV) PCR (QUALITATIVE)
Add:
Turnaround time
1 Jan 10
1 Jan 10
: 1 - 4 days; next working day for CSF samples
Delete entire test HLA-B27 TYPING BY PCR (QUALITATIVE)
1 Jan 10
(This test is moved under ALPHABETICAL LISTING – MISCELLANOUS
MOLECULAR TESTS page 195)
Delete entire test HUMAN INFLUENZA A & B PCR (QUALITATIVE)
26 Jan10
(This test is now incorporated together with Influenza A/H1N1/2009 as
INFLUENZA MULTIPLEX PCR – see below)
Delete entire test HUMAN INFLUENZA A/NOVEL H1N1 PCR (QUALITATIVE)
26 Jan 10
(This test is now incorporated together with Influenza A and B as
INFLUENZA MULTIPLEX PCR – see below)
26
37
38
4
4
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
MOLECULAR DIAGNOSTICS
192
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
MOLECULAR DIAGNOSTICS
193
After HUMAN PAPILLOMAVIRUS DNA (HPV) (QUALITATIVE)
26 Jan 10
Insert new test:
INFLUENZA MULTIPLEX PCR (QUALITATIVE)
Indications:
This test detects Influenza A, B and Influenza
A/H1N1/2009.
Specimen Required:
Nasal/ throat / nasopharyngeal swabs: Use
only Dacron-tipped or Copan flocked swab.
Send dry in sterile container or swab sheath.
Storage & Transportation:
Refrigerate sample until transfer to
laboratory. Do not freeze. Send sample at
2-8°C (with an ice-pack). Sample must
reach laboratory within 24 hours after
collection.
Method:
Multiplex reverse-transcription Polymerase
Chain Reaction (In-house real-time)
Test Results:
Detected, Not Detected or Inconclusive
Turnaround Time:
1 working day
Day(s) Test Set Up:
Monday to Saturday
Under NOROVIRUS PCR (QUALITATIVE)
Amend:
Turnaround time
Day(s) test set up
: 2 - 7 days
: Monday
To
Turnaround time
Day(s) test set up
: 1 - 5 days
: Tuesday and Thursday
1 Jan 10
27
39
40
41
4
4
4
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
MOLECULAR DIAGNOSTICS
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
MOLECULAR DIAGNOSTICS
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
MOLECULAR DIAGNOSTICS
193
194
195
Under PAN-ENTEROVIRUS PCR (QUALITATIVE)
Amend:
Turnaround time
Day(s) test set up
: 2 - 5 days
: Wednesday
To
Turnaround time
Day(s) test set up
: 1 - 4 days
: Wednesday and Friday
Under TOXOPLASMA GONDII PCR (QUALITATIVE)
Amend:
Turnaround time
Day(s) test set up
: 2 - 8 days
: Wednesday
To
Turnaround time
Day(s) test set up
: 1 - 4 days
: Variable
Under VARICELLA-ZOSTER VIRUS PCR (QUALITATIVE)
Amend:
Turnaround time
Day(s) test set up
: 1 – 7 days
: Wednesday
To
Turnaround time
Day(s) test set up
: 1 - 4 days
: Variable
1 Jan 10
1 Jan 10
1 Jan 10
28
42
4
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
MOLECULAR DIAGNOSTICS
195
After VARICELLA-ZOSTER VIRUS PCR (QUALITATIVE)
1 Feb 10
Insert:
ALPHABETICAL LISTING – MOLECULAR ONCOLOGY TESTS
Insert new test:
KRAS MUTATION TEST
Indications
: This assay detects 7 KRAS mutations in codons 12 and
13 (exon 2) of the KRAS oncogene. The presence of
these mutations correlates with a lack of response to
certain EGFR inhibitor cancer therapies in metastatic
colorectal cancer patients.
Specimen Required:
Paraffin embedded tissue block
Method:
Allele-specific real-time PCR assay employing
Amplification Refractory Mutation System (ARMS) PCR
Test Results:
Detected, Not Detected or Inconclusive
Turnaround Time:
3-15 days
Day Test Set Up:
Monday
Day Test Reported:
Thursday
29
43
4
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS:
MOLECULAR DIAGNOSTICS
195
After ALPHABETICAL LISTING – MOLECULAR ONCOLOGY TESTS
1Jan 2010
KRAS MUTATION TEST
Insert:
ALPHABETICAL LISTING –MISCELLANOUS MOLECULAR TESTS
Insert test:
HLA-B27 TYPING BY PCR (QUALITATIVE)
Specimen required:
Storage and transportation:
Method:
Test Result:
Turnaround Time:
Day(s)Test Set Up:
44
45
4
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS: Virology
223
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS: Virology
224
5 ml of whole blood in EDTA
Refrigerate sample until transfer to laboratory.
Do not freeze. Send sample at 2-8C. Sample
should reach the laboratory within 72 hours after
collection.
In-house real-time polymerase chain reaction
Detected, Not Detected or Inconclusive
2-8 days
Tuesday
Under CYTOMEGALOVIRUS (CMV) ANTIBODY
24 Nov 09
Amend
Turnaround time : Anti-CMV IgM : <1 – 8 days
Anti-CMV IgG : <1 – 4 days
Under DENGUE VIRUS ANTIBODY
24 Nov 09
Amend
Turnaround time : Anti-dengue IgM : <1 – 5 days
Anti-dengue total : 3 – 15 days
Day(s) test set up : Anti-Dengue total : Once a fortnight
30
46
47
48
49
50
51
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS: Virology
224
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS: Virology
226
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS: Virology
226
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS: Virology
227
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS: Virology
228
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS: Virology
228
Under EPSTEIN-BARR VIRUS (EBV) ANTIBODY
24 Nov 09
Amend
Turnaround time : Anti-VCA IgM/IgG : <1 – 4 days
Anti-VCA IgA
: <1 – 7 days
Anti-EA IgA
: <1 – 7 days
Under HEPATITIS C VIRUS (HCV) SEROLOGY
24 Nov 09
Amend
Turnaround time : Anti-HCV IgG : <1 – 2 days
Anti-HCV RIBA : <1 – 8 days
Under HEPATITIS DELTA VIRUS ANTIBODY (ANTI-HDV TOTAL AB)
24 Nov 09
Amend
Turnaround time : <1 – 7 days
Under HEPATITIS E VIRUS (HEV) ANTIBODY
24 Nov 09
Amend
Turnaround time : Negative report : <1 – 7 days
Under HERPES SIMPLEX VIRUS (HSV) ANTIBODY
24 Nov 09
Amend
Turnaround time : Anti-HSV IgM : Routine cases : <1 – 4 days
Under HUMAN IMMUNODEFICIENCY VIRUS (HIV) ANTIBODY, SCREENING
(includes Confirmation if reactive)
24 Nov 09
Amend
Turnaround time : Routine screening non-reactive report : <1 – 2 days
Confirmation of reactive results
: 1 – 8 days
31
52
53
54
55
56
57
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS: Virology
229
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS: Virology
229
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS: Virology
230
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS: Virology
231
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS: Virology
232
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS: Virology
233
Under HUMAN IMMUNODEFICIENCY VIRUS (HIV) ANTIBODY, WESTERN
BLOT
24 Nov 09
Amend
Turnaround time : 1 – 8 days
Under MEASLES ANTIBODY
24 Nov 09
Amend
Turnaround time : Anti-Measles IgM : <1 – 8 days
Anti-Measles IgG : <1 – 4 days
Under MUMPS ANTIBODY
24 Nov 09
Amend
Turnaround time : Anti-Mumps IgM or IgG : <1 – 4 days
Under PARVOVIRUS ANTIBODY
24 Nov 09
Amend
Turnaround time : IgM or IgG : <1 – 7 days
Under RUBELLA ANTIBODY
24 Nov 09
Amend
Turnaround time : 1 – 8 days
Anti-Rubella IgM or IgG : <1 – 7 days
Anti-Rubella total
: 1 – 8 days
Under SARS-CORONAVIRUS (SARS-COV) ANTIBODY
24 Nov 09
Amend
Turnaround time : Anti-SARS IgM or IgG : <1 – 4 days
32
58
59
60
61
62
63
64
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS: Virology
233
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS: Virology
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS: Virology
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS: Virology
234
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS: Virology
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS: Virology
SAMPLE COLLECTION &
HANDLING – SPECIAL
INSTRUCTIONS, LABORATORY
TESTS, REFERENCE VALUES
& TEST RESULTS: Virology
235
Under VARICELLA-ZOSTER VIRUS (VZV) ANTIBODY
24 Nov 09
Amend
Turnaround time : Routine cases :
Anti-VZV IgM : <1 – 4 days
Anti-VZV IgG : <1 – 7 days
Under CYTOMEGALOVIRUS ANTIGEN
24 Nov 09
Amend
Turnaround time : <1 – 2 days
234
Under DENGUE VIRUS ANTIGEN
24 Nov 09
Amend
Turnaround time : <1 – 4 days
234
Under HERPES SIMPLEX VIRUS AND VARICELLA-ZOSTER VIRUS
ANTIGEN
24 Nov 09
Amend
Turnaround time : >1 – 2 days
Under MEASLES AND MUMPS ANTIGEN
24 Nov 09
Amend
Turnaround time : <1 – 2 days
235
Under NOROVIRUS ANTIGEN
24 Nov 09
Amend
Turnaround time : <1 – 7 days
235
Under RESPIRATORY VIRUS ANTIGEN
24 Nov 09
Amend
Turnaround time : <1 – 2 days
33
65
7
TEST INDEX
276
After INTERPHASE FLUORESCENCE IN SITU HYBRIDIZATION (FISH)
PANELTEST FOR GLIOMA
1 Mar 10
Insert New FISH test: INTERPHASE FLUORESCENCE IN SITU
HYBRIDIZATION (FISH) PANEL TESTS FOR MULTIPLE MYELOMA,
MULTIPLE MYELOMA HYPERDIPLOIDY, B-CELL CHRONIC LYMPHOCYTIC
LEUKAEMIA (B-CLL) AND MYELODYSPLASTIC SYNDROME (MDS)
66
7
TEST INDEX
276
After INTERPHASE FLUORESCENCE IN SITU HYBRIDIZATION (FISH)
PANEL TESTS FOR MULTIPLE MYELOMA, MULTIPLE MYELOMA
HYPERDIPLOIDY, B-CELL CHRONIC LYMPHOCYTIC LEUKAEMIA (B-CLL)
AND MYELODYSPLASTIC SYNDROME (MDS
1 Mar 10
Insert new FISH test: INTERPHASE FLUORESCENCE IN SITU
HYBRIDIZATION (FISH) TEST FOR HAEMATOLOGICAL MALIGNANCIES
67
7
TEST INDEX
276
After INTERPHASE FLUORESCENCE IN SITU HYBRIDIZATION (FISH) TEST
FOR HAEMATOLOGICAL MALIGNANCIES
1 Mar 10
Insert new FISH test: INTERPHASE FLUORESCENCE IN SITU
HYBRIDIZATION (FISH) TEST FOR LYMPHOMA AND SARCOMA ON
PARAFFIN-EMBEDDED SECTIONS OR TISSUE IMPRINTS
68
7
TEST INDEX
275
Before CYTOMEGALOVIRUS (CMV) PCR (QUALITATIVE)
15 Dec 09
Insert new test:
CYTOMEGALOVIRUS (CMV) LOAD (QUANTITATIVE)
69
7
TEST INDEX
276
Before INFLUENZA VIRUS ISOLATION
26 Jan 10
Insert new test:
INFLUENZA MULTIPLEX PCR (QUALITATIVE)
34
70
7
TEST INDEX
277
After KETONE, BLOOD
1 Feb 10
Insert new test:
KRAS MUTATION TEST
35