Standard Operating Procedures
Laboratory Specific
Chemical: 2,4 - Dinitrophenol
Please fill out the form completely. Print a copy and insert into your
Laboratory Safety Manual and Chemical Hygiene Plan.
Refer to instructions for assistance.
_____________________________________________________________________________
Department:________________________
Date when SOP was written:_______
Date when SOP was approved by the lab supervisor: ___________________
Principal Investigator:___________________________________________________
Internal Laboratory Safety Coordinator/Lab Manager:___________________________________
Laboratory Phone:____________________ Office Phone:_____________________
Emergency Contact:____________________________________________________
(Name and Phone Number)
Location(s) covered by this SOP:__________________________________________
(Building/Room Number)
_____________________________________________________________________________
Type of SOP:
Process
Hazardous Chemical
Hazardous Class
Purpose
2,4-Dinitrophenol is a yellow, crystalline solid that has a sweet, musty odor. 2,4-DNP is
considered an important environmental contaminant by the United States Environmental
Protection Agency. It has been found in 61 of 1400 priority sites that need clean-up of industrial
waste. It can enter the air from automobile exhaust, burning of certain industrial substances, and
from reaction of nitrogen in air with other atmospheric chemicals. The major site of degradation is
the soil, where microorganisms metabolize it. Commercial 2,4-DNP is primarily used for scientific
research and in manufacturing. It has been used at times to make dyes, other organic chemicals,
and wood preservatives. It has also been used to make photographic developer, explosives, and
pesticides.
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Physical & Chemical Properties/Definition of Chemical Group
CAS#:
7732-18-5
Class:
Toxic chemical and potentially explosive when dry
Molecular formula:
C6H4N2O5
Boiling Point:
108 deg C Decomposition temperature is Not available
Melting Point:
113-115 deg C
Potential Hazards/Toxicity
EMERGENCY OVERVIEW: Appearance: Yellow, crystalline solid that has a sweet, musty
odor
Danger! Flammable solid. May be fatal if swallowed. Causes eye and skin irritation. Explosive
when dry. Causes respiratory tract irritation. Marine pollutant. May be fatal if inhaled. Harmful if
absorbed through the skin.
Target Organs: Kidneys, heart, central nervous system, liver, reproductive system
Potential Health Effects:
Eye: Causes eye irritation. A worker accidentally sprayed dinitrophenol into his eye. Chemical
conjunctivitis developed and it was treated with Blinex, Neosporin ophthalmic ointment, and an
eye patch. His vision was impaired for one month.
Skin: Causes skin irritation. Harmful if absorbed through the skin. 2,4-Dinitrophenol causes
maculopapular dermatitis. Dermatitis may be due to either primary irritation or allergic sensitivity.
Ingestion: May be fatal if swallowed. The metabolic rate of the poisoned individual can increase
markedly, and the body temperature is elevated. Dinitrophenol exerts its toxic effects by a general
disturbance of cell metabolism resulting in a need to consume excessive amounts of oxygen in
order to synthesize the essential adenine nucleotide required for cell survival in the brain, heart,
and muscles.
Inhalation: May be fatal if inhaled. Causes respiratory tract irritation. May cause effects similar
to those described for ingestion. Signs and symptoms of acute poisoning in humans include
nausea, restlessness, flushed skin, sweating, rapid respiration, tachycardia, fever, cyanosis, and
finally, collapse and coma. If the acute phase of poisoning is survived, the patient usually
tolerates later complications, which may include renal insufficiency and toxic hepatitis.
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Chronic: Prolonged or repeated skin contact may cause dermatitis. May cause liver and kidney
damage. May cause reproductive and fetal effects. 2,4-Dinitrophenol signs and symptoms are
fever/hyperthermia, skin discoloration, acidosis (metabolic, delayed), hypotension, cataract
(subscapular), hearing impairment (delayed).
Personal Protective Equipment (PPE)
Eyes: Wear chemical safety glasses
Skin: Viton gloves are highly preferred to prevent skin exposure.
Clothing: Wear long pants, closed toed shoes and a lab coat
Respirators: Follow the OSHA respirator regulations found in 29 CFR 1910.134 or European
Standard EN 149. Always use a NIOSH or European Standard EN 149 approved respirator when
necessary.
Engineering Controls
Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety
shower. Use adequate ventilation to keep airborne concentrations low. 2,4-dinitrophenol must
strictly be only used in a fume hood. It is never to be used outside of the fume hood.
First Aid Procedures
Eyes: In case of contact, immediately flush eyes with plenty of water for at least 15 minutes. Get
medical aid.
Skin: In case of contact, immediately flush skin with plenty of water for at least 15 minutes while
removing contaminated clothing and shoes. Get medical aid immediately. Wash clothing before
reuse.
Ingestion: Call the poison control center at 1-800-222-1222. If swallowed, do not induce
vomiting unless directed to do so by medical personnel. Never give anything by mouth to an
unconscious person. Get medical aid.
Inhalation: POISON material. If inhaled, get medical aid immediately. Remove victim to fresh
air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen.
Notes to Physician: Treat symptomatically and supportively.
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Special Handling and Storage Requirements
Handling: Wash thoroughly after handling. Wash hands before eating. Remove contaminated
clothing and wash before reuse. Use only in a well-ventilated area. Ground and bond containers
when transferring material. Do not get in eyes, on skin, or on clothing. Empty containers retain
product residue, (liquid and/or vapor), and can be dangerous. Do not ingest or inhale. Store
protected from light. Do not pressurize, cut, weld, braze, solder, drill, grind, or expose empty
containers to heat, sparks or open flames.
Storage: Store in a cool place in the original container and protect from sunlight. Store in a tightly
closed container. Material can ignite if dry. Do not allow material to completely dry. Keep
container closed to prevent drying out.
Spill and Accident Procedure
Chemical Spill Dial 911 and x59797
Spill – Vacuum or sweep up material and place into a suitable disposal container. Reduce
airborne dust and prevent scattering by moistening with water. Clean up spills immediately,
observing precautions in the Protective Equipment section. Avoid generating dusty conditions.
Remove all sources of ignition. Use a spark-proof tool. If the material is dry, explosives experts
may be necessary to dispose of the spill. Provide ventilation.
Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate
personal protective equipment and clean-up material for chemical spilled. Double bag spill waste
in clear plastic bags, label and take to the next chemical waste pick-up.
Large (>1 L) – Dial 911 (or 310-825-1491 from cell phone) and EH&S at x59797 for
assistance.
Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in
emergency shower for at least 15 minutes. Seek medical attention. Notify supervisor and EH&S
at x59797 immediately.
Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with
water for 15 minutes by forcibly holding the eye open. Seek medical attention. Notify supervisor
and EH&S at x59797 immediately.
Medical Emergency Dial 911 or x52111
Life Threatening Emergency, After Hours, Weekends And Holidays – Dial 911
(or 310-825-1491 from cell phone) or contact the Ronald Reagan UCLA Medical Center
(emergency room) directly at x52111 (located at 757 Westwood Plaza, enter from Gayley
Avenue). Note: All serious injuries must be reported to EH&S at x59797 within 8 hours.
Non-Life Threatening Emergency– Go to the Occupational Health Facility (OHF),
x56771, CHS room 67-120 (This is on the 6th floor, 7th corridor, room 120. Enter through the
School of Dentistry on Tiverton Drive and proceed to the “O” elevator to the 6th floor.)Hours: M F, 7:30 a.m. to 4:30 p.m. At all other times report to Ronald Regan UCLA Medical Center
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(emergency room) at x52111. Note: All serious injuries must be reported to EH&S at x59797
within 8 hours.
Needle stick/puncture exposure (as applicable to chemical handling procedure)– Wash
the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane
exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick
nurse by dialing 231 from a campus phone, enter 93333 when prompted and then enter your
extension. Hours: M – F, 8:00 a.m. to 4:00 p.m. At all other times report to Ronald Regan UCLA
Medical Center (emergency room) at x52111. Note: All needle stick/puncture exposures must be
reported to EH&S at x59797 within 8 hours.
Decontamination/Waste Disposal Procedure
Waste disposal procedures
Vacuum or sweep up material and place into a suitable disposal container. Reduce airborne dust
and prevent scattering by moistening with water. Clean up spills immediately, observing
precautions in the Protective Equipment section. Avoid generating dusty conditions. Remove all
sources of ignition. Use a spark-proof tool. If the material is dry, explosives experts may be
necessary to dispose of the spill. Provide ventilation.
Material Safety Data Sheet (MSDS) Location
(State the location of MSDS)
Hardcopy or electronic copy must be available.
Online MSDS can be accessed at http://msds.ehs.ucla.edu.
Protocol/Procedure
(Add specific description of procedure.)
Note: Any deviation from this SOP requires written approval from PI.
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Documentation of Training (signature of all users is required)
I have read and understand the content of this SOP:
Name
Signature
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Date