PH.D. RESUME 1
Mary Doe
1000 Elm Rd. Apt. 1  College Station, Texas 77840  (979) 555-4445  mdoe@dept.tamu.edu
OBJECTIVE
Seeking a position as a researcher using background in both biochemistry and chemistry
SUMMARY OF QUALIFICATIONS
 Three years research experience in enzyme kinetics.
 Research interests: biochemistry, pharmaceutical science, medicine, veterinary medicine, and chemistry.
 Able to work independently or as part of a team.
EDUCATION
Texas A&M University, College Station, TX
Ph.D., Biochemistry; GPR 3.93
Expected: August 2005
Big State University, Middletown, IN
M.S., Chemistry, GPR 3.89
B.S., Pharmacy
August 2002
July 1993
RELEVANT COURSEWORK
 Enzymes, Proteins, and Nucleic Acids
 Biochemical Kinetics; Biochemical Genetics
 Pharmacology; Pharmaceutics
RESEARCH EXPERIENCE
Research Assistant, Texas A&M University, College Station, TX
August 2002- present
 Incorporated Tyrptophan (Trp) Analogs into the Phosphofructokinase from Bacillus stearothermophilus (BsPFK).
 Studying the mechanisms of a chemotaxis system (Transmembrane Serine Receptor, Tsr) in Escherichia coli.
 Measured ligand-binding properties (dissociation constant and stoichiometry) of the periplasmic domain of Tsr
using both fluorescence spectrometry and isothermal titration calorimetry.
 Determined the oligomeric state of periplasmic domain of Tsr using analytical ultracentrifugation and analytical
gel filtration.
 Developed a spectrophotometric method using proteins on native cell membrane.
 Studied the inhibition effect of ligand-bound Tsr on a complexed histidine kinase activity using coupled enzyme
activity assay and radioactive assay.
Research Assistant, Big State University, Middletown, IN
March 2001- August 2002
 Developed a new animal model for cardiovascular research in space: transparent tadpoles outfitted with in vivo
nitric oxide sensors.
 Thesis: “A Chemically Modified Carbon Fiber-Based Nitric Oxide Sensor for Biological Applications”
TECHNICAL SKILLS
Scientific:
 PCR primer design, polymerase chain reaction (PCR), reverse transcription, subclone PCR product into cloning
vector.
 Plasmid construction, enzyme digestion, point mutation, amplification and extraction.
 DNA and RNA puification, elctrophoresis, Northern blot, and Southern blot.
 Construction of bacteria strain, gene knockout, Construction of expression vector, transformation, Cell Culture,
Protein overexpress and purification.
 SDS-PAGE, Western blot, BCA assay, enzyme kinetic assays.
 FPLC, HPLC, analytical ultracentrifugation, spectrophotometry, fluorescence spectrometry, analytical gel
filtration, isothermal titration calorimetry.
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PH.D. RESUME 1
Computer:
 Proficient in using Internet tools to do DNA, RNA and Protein analysis, e.g. Blast etc; to conduct database and
journal search, e.g. Medline.
 Skilled at using some computer programs to visualize and analyze protein structures, e.g. Rasmo; to do protein or
DNA sequence comparison, e.g. Macvec; and to analyze and fit experimental data, e.g. KaleidaGraph, Origin,
ultrascan and Sedfit etc.
 Sufficient in working with computer (Mac, PC, Microsoft Office).
INDUSTRY EXPERIENCE
Texas A&M University, College Station, TX
August 2002- present
Teaching Assistant
 Taught undergraduate biochemistry recitation sections, graded papers, exams and homework, kept office hours.
Big State University, Middletown, IN
March 2001- August 2002
Teaching Assistant
 Taught and prepared for undergraduate chemistry labs, graded lab papers and exams, and kept office hours.
Big Box Discount Center, Smallville, IN
July 1993- August 1998
Pharmacy Tech
 Assisted pharmacist, trained and supervised new employees, and maintained pharmacy inventories
SELECTED PUBLICATIONS/PRESENTATIONS
Biophysical Society Annual Meeting, New York, NY, 2004
Poster: “Purification and Characterization of the Periplasmic Domain of Serine Chemoreceptor”
The Center for the Study of Biochemistry, Noname, TX, August 2004
Poster: “Purification and Characterization of the Periplasmic Domain of Serine Chemoreceptor”
The Center for the Study of Biochemistry, Noname, TX, August 2003
Poster: “Incorporation of Tryptopan (Trp) Analogs into the Phosphofructokinase from Bacillus stearothemophilus
(BsPFK)”
HONORS & AWARDS
Fellowship A
Scholarship B
Outstanding Graduate Student, Dept. of XXX, Texas A&M University
FOREIGN LANGUAGE SKILLS
Proficient written and spoken Spanish
PROFESSIONAL MEMBERSHIPS
American Society of Biochemistry
Southern Association of Biochemistry
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