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Iranian Biomedical Journal, ( ISI ), Year (2003-1)
Title : ( immunization of sheep with phage mimotopes against dermatophilosis )
GHOLAMREZA HASHEMI TABAR ,
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Abstract
Random peptide libraries (RPLs) displayed on the surface of filamentous bacteriophages have
been extensively used as a tool to map epitopes or to identify antigenic mimics (mimotpoes) of
disease-specific monoclonal antibodies or polyclonal sera. These RPLs are engineered by the
insertion of degenerate oligonucleotides, encoding a specific number of random amino acids, in
frame with a bacteriophage’s gene specifying a virion surface protein. The RPLs are constructed
by inserting 21 to 36 random nucleotides into the gene for an appropriate coat protein in a phage
which is propagated in E. coli. Particularly clear mimotopes have been obtained with monoclonal
antibodies and with a few polyclonal antibodies against viruses. The choice and processing of
sera for selection of disease related mimotopes and sera to remove mimotopes reacting with
ubiquitous antibodies is important in studies of RPLs. We have used RPL to select mimotopes of
antigens to be used for immunization against dermatophilosis. Immunoglobulin G (IgG) from
sheep protected from dermatophilosis with an enzyme preparation from Dermatophilus
congolensis, were used to select peptides displayed on phage in the Ph.D.-7 random peptide
library which contains 109 peptides. Phage displaying peptides which were unrelated to the
mimotopes associated with protection to dermatophilosis were removed with IgG from sheep
which were vaccinated with the enzyme preparation but were not protected. The IgG from the
protected sheep before vaccination was also used for negative selection. The selected mimotopes,
those with clearly repeating motifs were chosen and used to immunize sheep. A mixture of four
phage mimotopes induced antibody to a recombinant protease from D. congolensis. The
immunized sheep, recovered more rapidly from the lesions caused by the strains when
challenged with two strains of D. congolensis. Key words: Phage display, Random peptide
library, Dermatophilosis, Serine protease
‫ک لمات ک ل یدی‬
, phage display, random peptide library, dermatophilosis, serine
‫ب رای دا‬.‫ن لود از ش نا سه و رمز ع بور پ رت ال پ وی ا ا س ت فاده ک ن ید‬
‫ش نا سه‬
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BibTeX
EndNote
@article{paperid:201262,
author = {HASHEMI TABAR, GHOLAMREZA},
title = {immunization of sheep with phage mimotopes against dermatophilosis},
journal = {Iranian Biomedical Journal},
year = {2003},
month = {January},
issn = {1028-852x},
keywords = {phage display; random peptide library; dermatophilosis; serine protease},
}
[Download]
%0 Journal Article
%T immunization of sheep with phage mimotopes against dermatophilosis
%A HASHEMI TABAR, GHOLAMREZA
%J Iranian Biomedical Journal
‫‪%@ 1028-852x‬‬
‫‪%D 2003‬‬
‫]‪[Download‬‬
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