1.
What was the first class of tandem repeats used in forensic casework? a.
AFLP b.
STR c.
Minisatellites d.
PCR
2.
Another name for minisatellites is: a.
Variable-Number Tandem Repeats b.
Amplified Fragment Length Polymorphisms c.
Population Match Probability d.
Polymerase Chain Reaction
3.
In VNTRs, the genotype is defined by a particular number of ________________ at a given locus. a.
Population match probabilities b.
Minisatellites c.
Alleles d.
Tandem repeats
4.
The discrimination power of VNTR loci used for forensic testing is measured by which of the following? a.
Random Match Probability b.
Variable-Number Tandem Repeats c.
Amplified Fragment Length Polymorphisms d.
Polymerase Chain Reaction
5.
The ______________ the random match probability, the less likely a match will occur between two randomly chosen individuals. a.
Higher b.
Lower c.
More likely d.
None of the above
6.
Which of the following is involved in the Restriction Fragment-Length Polymorphism method? a.
Southern transfer and hybridization b.
Genomic DNA preparation c.
Agarose gel electrophoresis d.
All of the above
7.
Which of the following RFLP methods was used in North American labs? a.
HinfI-based RFLP b.
HaeIII-based RFLP c.
PstI d.
HaeII-based VNTR
8.
In RFLP, this method can be used to transfer DNA to a filter so that it can be detected with a probe: a.
Hybridization b.
VNTR c.
PCR d.
Southern Blotting

9.
In VNTR analysis, the use of a __________ results in a complex barcode-like band pattern generated from multiple
VNTR loci: a.
Single-locus probe b.
Multi-locus probe c.
Southern Blotting d.
None of the above
10.
This technique used in VNTR analysis was pioneered by Sir Alec Jeffreys in 1984: a.
Single-locus probe b.
Multi-locus probe c.
Southern Blotting d.
PCR
11.
Multi-locus probe approaches have been very successful in which of the following cases? a.
A cold case with degraded DNA b.
A rape with a very small DNA sample c.
A dispute over parentage d.
A robbery
12.
Single-locus probes generate a simple pattern called a: a.
DNA Profile b.
Genetic Fingerprint c.
DNA Fingerprint d.
All of the above
13.
The first case to utilize DNA evidence in a criminal investigation occurred in the United Kingdom in what year? a.
1990 b.
1983 c.
1980 d.
1992
14.
In VNTR analysis, the bands of similar size are grouped into what? a.
Bins b.
Loci c.
Alleles d.
Profiles
15.
In VNTR analysis, a conclusion that indicates “Inclusion” means which of the following? a.
The profiles are different b.
The profiles match c.
No conclusion can be made d.
The samples are from different sources
16.
In RFLP profiling, DNA degradation can be detected using which of the following? a.
Agarose gel electrophoresis b.
Partial restriction digestion c.
PCR analysis d.
All of the above
17.
This term refers to the deviation of the specificity of the cleavage site of a restriction enzyme: a.
Point mutation b.
Point degradation c.
Partial stripping d.
Star activity
18.
The substitution, deletion, or insertion of a single nucleotide in a DNA sequence is called a.
A point mutation b.
Point degradation

c.
Partial stripping d.
Star activity
19.
Which of the following loci was used by forensic DNA labs for amplified fragment length polymorphisms? a.
D1S80 b.
D1S99 c.
T1S80 d.
DS1980
20.
Which of the following cases would be suitable for AFLP analysis? a.
A cold case with a degraded sample b.
A paternity case c.
A case with very limited DNA in the sample d.
All of the above