Alexandria Faculty of Medicine
Clinical Genomics Center
‫اإلســتـخدام‬
‫أســــم الجهــــاز‬
‫م‬
Nucleic Acid Visualization on Ethdium Bromide stained gels
UV-Transiluminator
1
Agarose gel electrophoresis for detection of nucleic acid and PCR products
Submarine Mini Gel
Electrophoresis
2
Used in Electrophoresis
Electrophoresis Power Supply
3
Sterilization of Lab ware
Autoclave
4
For Thermal adjusted incubations
Water Bath
5
Thermal Cycler “Bio Cycler”
(PCR)
6
For automated thermal cycling (PCR)
Wallac System
 In Biochemical Genetics for screening of metabolic disorders such as :
Phenyl Ketonuria , Galactosemia , Cystic fibrosis
 Hormonal assay – Prenatal screening assay :
1. First Trimester ( B HCG - PAPP-A )
2. Second Trimester (AFP - HCG - Eu3)
1. Wallac VICTOR 2 D +
adjoined computer
(fluorescent plate
redden)
7
2. Automatic Dispenser
Guthrie card automated puncher for reliability & high through put newborn
Screening
3. Puncher
4. Washer & Remover
Newborn and Prenatal screening protocols
96 well – plate thermal incubator & shaker
5. Incubator
12 Capillary semi-quantitative electrophoresis for high through put analysis of
nucleic acid up to 95 samples in single run
Nucleic Acid Fragment
Analyzer e-Gene
8
Protein electrophoresis + Power
Supply + canon scanner
9
For Hb electrophoresis and diagnosis of hereditary hemoglobinopathies
(carriers & cases)
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Distillator
10
Mini – Rocker MR-1
11
Vortex
12
For solution preparation
Magnetic Stirrer
13
Weighing chemicals
Sensitive Balance
14
Biological Safety Cabinet
15
Freezer -85⁰C
16
Freezer -75⁰C
17
pH meter
18
Microwave
19
Micro Centrifuge
20
Centrifuge
21
Fume Cabinet
22
3 computers
23
For automated thermal cycling (PCR)
Gene Amplification PCR System
9700
24
Sequencer
Genetic Analyzer 3500
25
Gel imaging System
26
UV Transilluminator
27
For water distillation
Rocking shaking for staining trays
Vortexing for adequate mixing & suspension preparation
Class II safety cabinet for hygienic handling of biological samples
Related to research projects & establishing Biobanking
Measuring PH
Nucleic Acid Visualization on Ethdium Bromide stained gels
Agarose gel electrophoresis for detection of nucleic acid and PCR products
Horizontal gel – Electrophoresis
28
Used in Electrophoresis
Power pack 300 volts
29
Real –time PCR
Real-time Quantitative PCR
System 7500
30