Tutorial Protein Set 2 Matching A) B) C) D) E) F) G) H) I) J) K) L) M) uncharged deprotonated three -amino group two C-terminus lysine racemic mixture zero protonated isoleucine tryptophan phenylalanine 1. When a peptide bond is formed, an ______ reacts with a carboxylate group. Ans: D Section 4.1.B Level of Difficulty: Easy Learning objective: Amino Acid Structure 2. In the peptide Trp-Ser-Val, valine is at the ______. Ans: F Section 4.1.E Level of Difficulty: Easy Learning objective: Amino Acid Structure 3. At a pH above its pK, the phenolic group of tyrosine is ______. Ans: B Section 4.1.C Level of Difficulty: Easy Learning objective: Amino Acid Structure 4. At a pH below its pK, the Ans: J -amino group of lysine is ______. Section 4.1.D Level of Difficulty: Easy Learning objective: Amino Acid Structure 5. At a pH below its pK Ans: A or J -carboxylate group of Asp is ______. Section 4.1.D Level of Difficulty: Easy Learning objective: Amino Acid Structure 6. The net charge of the zwitterion form of Gly is ______. Ans: I Section 4.1.A Level of Difficulty: Easy Learning objective: Amino Acid Structure 7. Biological systems usually produce a single enantiomer, whereas chemical synthesis produces a ______. Ans: H Section 4.2 Level of Difficulty: Easy Learning objective: Amino Acid Structure 8. In the tripeptide Trp-Val-Phe, the N-terminal residue is ______. Ans: L Section 4.1.E Level of Difficulty: Easy Learning objective: Amino Acid Structure 9. In the tripeptide Lys-Pro-Ile, the C-terminal residue is _________. Ans: K Section 4.1.E Level of Difficulty: Easy Learning objective: Amino Acid Structure 10. In the tripeptide Lys-Pro-Ile, there are ______ charged groups at pH 7. Ans: C Section 4.1.C Level of Difficulty: Easy Learning objective: Amino Acid Structure Multiple Choice 11. Which of the following amino acids has a charged polar side chain at pH 7? A) Leu B) Ala C) Met D) Trp E) Glu Ans: E Section 4.1.C Level of Difficulty: Easy Learning objective: Amino Acid Structure 12. Which of the following amino acids has a sulfur atom in its side chain? A) Asn B) Ser C) Phe D) Met E) Tyr Ans: D Section 4.1.C Level of Difficulty: Easy Learning objective: Amino Acid Structure 13. Which of the following amino acids has an uncharged polar side chain at pH 7? A) Arg B) Thr C) Glu D) Pro E) Ile Ans: B Section 4.1.C Level of Difficulty: Easy Learning objective: Amino Acid Structure 14. At pH 7, arginine (pK -amino 8.99, guanidino 12.48) would be charged as follows: -amino, +1 guanidino, +1 net charge -amino, -1 guanidino, 0 net charge -carboxylate, -amino, -1 guanidino, -1 net charge D) -amino, +1 guanidino, +1 net charge E) -amino, +1 guanidino, 0 net charge Ans: D Section 4.1.C Level of Difficulty: Moderate Learning objective: Amino Acid Structure 15. At pH 7, aspartic acid (pK -carboxylate 3.9 ) would be charged as follows: A) 0 -carboxylate, +1 -amino, 0 -carboxylate, +1 net charge B) -1 -carboxylate, +1 -amino, -1 -carboxylate, -1 net charge C) 0 -carboxylate, -1 -amino, 0 -carboxylate, -1 net charge D) +1 -carboxylate, -1 -amino, +1 -carboxylate, +1 net charge E) +1 -carboxylate, +1 -amino, +1 -carboxylate, +3 net charge Ans: B Section 4.1.C Level of Difficulty: Moderate Learning objective: Amino Acid Structure 16. At pH 11, glutamic acid (pK would be charged as follows: A) +1 -carboxylate, 0 -amino, +1 -carboxylate, +2 net charge B) -1 -carboxylate, +1 -amino, -1 -carboxylate, -1 net charge C) 0 -carboxylate, 0 -amino, 0 -carboxylate, 0 net charge D) +1 -carboxylate, -1 -amino, +1 -carboxylate, +1 net charge E) -1 -carboxylate, 0 -amino, -1 -carboxylate, -2 net charge Ans: E Section 4.1.C -carboxylate 4.07. Level of Difficulty: Moderate Learning objective: Amino Acid Structure 17. At pH 4, histidine (pK -amino 9.33, 6.04 imidazole) would be charged as follows: -amino, -1 imidazole, 0 net charge B) -amino, 0 imidazole, 0 net charge -amino, -1 imidazole, +1 net charge D) -amino, +1 imidazole, +1 net charge -amino, +1 imidazole, +2 net charge Ans: D Section 4.1.C Level of Difficulty: Moderate Learning objective: Amino Acid Structure 18. At pH 5, cysteine (pK -amino 10.7, sulfhydryl 8.37 ) would be charged as follows: -amino, 0 sulfhydryl, 0 net charge -carboxylate, -amino, -1 sulfhydryl, -1 net charge C) -amino, +1 sulfhydryl, +1 net charge D) -amino, 0 sulfhydryl, 0 net charge E) +1 -carboxylate, -1 -amino, 0 sulfhydryl, 0 net charge Ans: D Section 4.1.C Level of Difficulty: Moderate Learning objective: Amino Acid Structure 19. At pH 1, lysine (pK charged as follows: -carboxylate, B) C) +1 -carboxylate, +2 D) 0 -carboxylate, +1 E) +2 -carboxylate, +1 - - -amino, -amino, -2 net charge -amino, +1 net charge -amino, +2 -amino, +5 net charge -amino, +1 -amino, +2 net charge -amino, +1 -amino, +4 net charge Ans: D Section 4.1.C Level of Difficulty: Moderate Learning objective: Amino Acid Structure -amino 10.54 ) would be 20. The disulfide bond between two cysteine molecules: A) is a peptide bond. B) is an ionic bond that is stable at physiological pH. C) is a covalent bond formed by oxidation. D) is a hydrogen bond between the two sulfhydryl groups. E) is a weak ion-induced dipole attraction. Ans: C Section 4.1.C Level of Difficulty: Easy Learning objective: Amino Acid Structure 21. What proportion of the 20 amino acids are considered to be nutritionally essential to lab animals? A) 0% B) 25% C) 50% D) 75% E) 100% Ans: C Section 4.1 Level of Easy: Learning objective: Amino Acid Structure 22. Which of the following statements is/are true? A) amino acids can be derived from purines and pyrimidines B) purines and pyrimidines can be derived from amino acids C) no protein contains every standard amino acid D) A and C E) B and C Ans: B Section 4.1 Level of Difficulty: Easy Learning objective: Amino Acid Structure 23. The isoelectronic point of an amino acid is the point where: A) The pK of the carboxylic acid is the same as the amino group B) Sodium ions are attracted to the amino groups C) the solubility of the amino acid is maximized D) the amino acid racemizes E) the amino acid carries no net electrical charge Ans: E Section 4.1.D Level of Difficulty: Moderate Learning objective: Amino Acid Structure 24. Amino acid side-chain residues have: A) a positive charge in every situation B) pKs that assure the solubility of every protein C) constant pKs no matter what aqueous environment they are found in D) different pKs in peptides as compared to the free amino acids E) polar functional groups Ans: D Section 4.1.D Level of Difficulty: Moderate Learning objective: Amino Acid Structure 25. Asx refers to A) a negatively charged aspartic acid B) a positively charged asparagine C) a dipeptide containing both aspartic acid and asparagine D) either aspartic acid or asparagine E) an unnatural amino acid formed on protein hydrolysis Ans: D Section 4.1.E Level of Difficulty: Easy Learning objective: Amino Acid Structure 26. The formation of a dipeptide from two amino acids requires: A) side-chain complementarity B) loss of water -carbon -carbon E) base catalysis Ans: B Section 4.1.B Level of Difficulty: Easy Learning objective: Amino Acid Structure 27. The peptide AYDG has an N-terminal _________ residue. A) glycine B) glutamic acid C) glutamine D) aspartic acid E) alanine Ans: E Section 4.1.E Level of Difficulty: Easy Learning objective: Amino Acid Structure 28. Which of the following tripeptides would be expected to be the most hydrophobic: A) KYG B) KYA C) GYA D) DYA E) DYG Ans: C Section 4.1.E Level of Difficulty: Moderate Learning objective: Amino Acid Structure 29. What is the abbreviation for the peptide valylarginylisoleucine? A) Val-Arg-Ile B) Val-Agn-Leu C) Vln-Arg-Ile D) Val-Agn-Isl E) Vln-Arg-Leu Ans: A Section 4.1.E Level of Difficulty: Easy Learning objective: Amino Acid Structure 30. The least abundant amino acid in proteins is: A) V B) W C) G D) F E) A Ans: B Section 4.1 Level of Difficulty: Moderate Learning objective: Amino Acid Structure 31. Zwitterions are: A) amino acids B) ionic molecules that are racemic C) molecules that bear both negatively and positively charged groups D) side chain carboxylate anions E) delocalized ions Ans: C Section 4.1.A Level of Difficulty: Easy Learning objective: Amino Acid Structure 32. All the standard amino acids except ____ are optically active. A) Pro B) Arg C) Trp D) Gly E) Phe Ans: D Section 4.2 Level of Difficulty: Easy Learning objective: Amino Acid Structure 33. The __________ character of most standard amino acids causes solutions of amino acids to rotate plane polarized light. A) chiral B) zwitterionic C) polar D) all of the above E) none of the above Ans: A Section 4.2 Level of Difficulty: easy Learning objective: Amino Acid Structure 34. All amino acids derived from proteins have the same conformation as: A) L-glyceraldehyde B) dextro-glyceraldehyde C) D-glucose D) cis-lysine E) glycine Ans: A Section 4.2 Level of Difficulty: Easy Learning objective: Amino Acid Structure 35. Typically, ‘unnatural’ amino acids with side chain modifications A) are synthesized due to genetic mutations B) are assembled by specialized bacterial enzymes C) are formed by modification of standard side chain residues after the protein synthesis D) are toxic E) are racemic Ans: C Section 4.3.A Level of Difficulty: Moderate Learning objective: Amino Acid Structure 36. An amide bond between a sideA) disulfide bond B) amino amide bond C) nor-peptide bond D) isopeptide bond E) hormone bond -amino group is also called a(n): Ans: D Section 4.3 Level of Difficulty: Moderate Learning objective: Amino Acid Structure Short Answer 39. Why can amino acid-binding proteins distinguish the enantiomeric forms of the free amino acids? Ans: Proteins consist of L-amino acids, so their binding sites are also chiral and can therefore recognize a particular enantiomer of an amino acid. Level of Difficulty: Moderate Learning objective: Amino Acid Structure 38. Why do bacterial cell walls containing peptides with D-amino acids often escape digestion by the proteases that other organisms use to kill bacteria? Ans: The proteases only recognize L-amino acids. Level of Difficulty: Moderate Learning objective: Amino Acid Structure Fill in the Blank 39. Histamine is formed from histidine by a ________ reaction. Ans: decarboxylation Section 4.3.A Level of Difficulty: Moderate Learning objective: Amino Acid Structure 40. _________ is a neurotransmitter formed from tyrosine by hydroxylation and decarboxylation reactions. Ans: Dopamine Section 4.3.B Level of Difficulty: Moderate Learning objective: Amino Acid Structure 41. Proline differs from the other 19 amino acids in proteins in that it has a _________ amino group. Ans: secondary Section 4.1.C Level of Difficulty: Moderate Learning objective: Amino Acid Structure