Lab Report 3 – Exercise 7 Evaluation & Grading
Maximum Score = 50 points
_____ Title Page = 6 points
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Exercise #’s = 1 point
Title of Exercise in larger font = 2 points
Due date for lab report = 1 point
Identified as BIOL 100B – Spring 2005 = 1 point
Centered on Page = 1 point
_____ Purpose = 6 points
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Overall Purpose of Exercise 7 = 2 points
Expected results if Homozygous = 2 points
Expected results if Heterozygous = 2 points
_____ Materials & Methods = 6 points
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Summary of 7.1—Isolation of DNA methods & materials = 1 point
Summary of 7.2—PCR amplification of DNA (Must include volumes, concentrations, and composition of mixes) = 2 points
Summary of 7.3—Gel Electrophoresis methods & materials = 2 points
Summary of 7.4—Bioinformatics tools used online = 1 point
_____ Results = 6 points
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Identify samples in each lane of gel = 1 point
Label 100bp markers (requires that a diagram of gel is included) = 1 point
Approximate size of resulting bands for marker lane and expected number of bands for homozygotes & heterozygotes = 2 points
Verbal description of what should have gotten in the different lanes of gel including the marker lane = 2 points
_____ Discussion & Interpretation of Results = 20 points
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Overall = 6 points
_____ Brief introduction = 1 point
_____ Brief conclusion = 1 point
_____ Possible sources of error = 3 points
_____ Discuss contributions of Alu to genomic diversity = 1 point
PCR Homework = 6 points
_____ Purpose of PCR =1point
_____ How are DNA strands forced to separate? =1 point
_____ Primer (explain what it is) =1 point
_____ On which strands do primers anneal, and where do they anneal relative to region to be amplified? =1 point
_____ What occurs at each of the three temperatures of PCR (95, 50-56, and 70-74)? = 1 point
_____ How does quantity of DNA in target region change with each cycle? = 1 point
Sequencing Homework = 2 points
_____ Purpose of sequencing = 1 point
_____ How does sequencing differ from PCR (be specific!)? = 1 point
Analysis of Gel Electrophoresis = 4 points
_____ Was DNA in + control (see page 7-15) homozygous or heterozygous? Explain answer. = 1 point
_____ How many bands would you expect to see on the gel if you are homozygous? Explain answer. = 1 point
_____ How many bands would you expect to see on the gel if you are heterozygous? Explain answer. = 1 point
_____ Propose reasons as to how large differences in DNA can exist without influence on the resulting protein = 1 point
Bioinformatics Homework = 2 points
_____ Identify the chromosome gene is located on = 1 point
_____ Identify the function of that gene = 1 point
_____ Literature Cited = 5 points
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Minimum of 5 sources (BLAST, PCR Tutorial, Sequencing Tutorial, Campbell text & lab manual) = 1 point
Proper format of all citations both in the text of paper and on the Literature Cited page = 3 points
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New page titled ‘Literature Cited’ = 1 point
(every citation on Literature Cited page must be properly cited in text of paper; every cited item in the text must be listed on Literature Cited page)
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Points deducted (a maximum of 5 points will be deducted for any spelling and grammatical errors; 1 point deducted if page numbers not
typed or missing; 2 points deducted if margins greater or less than one inch; 1 point deducted if heading titles not boldface; 2 points
deducted if extensive description of methods and materials rather than a summary; 2 points deducted for improper tense in methods section;
1 point deducted for premature interpretation of results in the results section of paper).
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Points deducted for late submission (25 points will be deducted if this lab report is submitted after due date but within 1 week; 50 points
will be deducted if this lab report is submitted 1 week after the due date).
_____ Final Total (maximum = 50 points)