JOINT GENOMIC CENTER
8 Dragan Tsankov blvd, 1164 Sofia, Bulgaria
Е-mail: dna@jgc-bg.org
tel. +359 2/ 819 39 83
fax +359 2/ 819 39 88
Sequencing Request Form
№ … / …………..(dd/mm/yyyy)
Filled by the client
User information
First Name and Family Name of the Client:
University/Institute/Company:
Department/Section:
Tel:
Е-mail:
Fax:
Way of
payment:
Invoice
details:
Cash
Bank transfer
Prepaid account at JGC
account №
Joint project with JGC
account №
Contract with JGC
account №
For organizations registered in Bulgaria
For organizations registered outside Bulgaria
Organization:
Address:
BULSTAT:
VAT number:
Financially Liable Person:
Person who obtains the results from
analysis:
Organization:
Address:
VAT number:
Person who obtains the results from analysis:
Request submission date:
Filled by JGC
Sample received by:
Signature
Date
Notes
Sample analyzed by:
Signature
Date
Notes
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JOINT GENOMIC CENTER
8 Dragan Tsankov blvd, 1164 Sofia, Bulgaria
Е-mail: dna@jgc-bg.org
tel. +359 2/ 819 39 83
fax +359 2/ 819 39 88
Filled by the client:
Samples and Service Information
(www.jgc-bg.org for full description of the type of samples and services)
Sample
Required services
Purified DNA sample synthesized by:
ABI BigDye
ver. 3.1
Services: [A]
ver. 1.1
DNA sample synthesized by:
ABI BigDye
ver. 3.1
Services: [A, B]
ver. 1.1
Purified plasmid DNA
Services: [A, B] and
*sample prepared by BigDye
Purified PCR products
ver.3.1
ver.1.1
ver.3.1
ver.1.1
ver.3.1
ver.1.1
ver.3.1
ver.1.1
Services: [A, B] and
* sample prepared by BigDye
PCR reaction (unpurified PCR products)
Services: [A, B, C] and
* sample prepared by BigDye
Agarose gel slice containing DNA fragments
Services: [A, B, D] and
* sample prepared by BigDye
Services: [A]- DNA analysis by ABI 3130; [B]- Purification by BigDye XTerminator®; [C]- Purification of
PCR fragments; [D]- DNA purification from agarose gel;
PCR primers to be used for the sequencing reaction
‘Universal’ PCR primers free from JGC
Specific PCR primers provided from the client (please fill the name and the concentration of the primer)
Primer name
Concentration (pmol/µl)
Primer name
Concentration (pmol/µl)
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Filled by the client when submitting samples in PCR tubes!
Please, fill in sample names in the JGC Sequencing Sample List EN form when submitting the samples in a
96-well PCR plate!
Sample information 1
Sample name
1
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DNA
concent
ration
(ng/µl)
Sequencing primer
‘Universal’ JGC primers
M13F
M13R
T3
T7
M13F
M13R
T3
T7
M13F
M13R
T3
T7
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Specific primer
(supplied by client)
Comments
JOINT GENOMIC CENTER
8 Dragan Tsankov blvd, 1164 Sofia, Bulgaria
Е-mail: dna@jgc-bg.org
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M13F
M13R
T3
T7
M13F
M13R
T3
T7
M13F
M13R
T3
T7
M13F
M13R
T3
T7
M13F
M13R
T3
T7
M13F
M13R
T3
T7
M13F
M13R
T3
T7
M13F
M13R
T3
T7
M13F
M13R
T3
T7
M13F
M13R
T3
T7
M13F
M13R
T3
T7
M13F
M13R
T3
T7
M13F
M13R
T3
T7
M13F
M13R
T3
T7
M13F
M13R
T3
T7
M13F
M13R
T3
T7
M13F
M13R
T3
T7
M13F
M13R
T3
T7
M13F
M13R
T3
T7
tel. +359 2/ 819 39 83
fax +359 2/ 819 39 88
Copy/ paste more rows
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Detailed information regarding the procedure and terms of sample submission is available at the internet address of JGC (www.jgc-bg.org)
Notes:
- It is necessary to supply at least 10 µL of DNA solution for a single sequencing
- Give unique name to each sample. Spaces ARE NOT allowed in sample names. Please, use the symbol "_"
if necessary. Please, give short names to the samples, preferably consecutive numbers (e.g. 1, 2, 3…) in
order to secure efficient sample processing. Designate clearly the sample tubes.
- We DO NOT accept any sample with potential biohazard
- Submit all samples in a plastic bag with the current form attached to it
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