BIO 105, CRN 21684, Spring 2014 – Desert Ecology Lab DESERT ECOLOGY LAB In this lab, you will work with a partner to conduct your own, hands-on, scientific investigation, on a topic of your choice related to the ecology of the Sonoran Desert. You will gain experience and confidence in your ability to do science and think critically. You will also learn how to communicate scientific results both in writing and via oral presentation. CONTENTS: Page numbers Assignment descriptions and instructions: 1. List of questions 2. Draft proposal 3. Proposal 4. Data table 5. Written report 6. Presentation Grading rubrics: 1. Proposal and written report grading rubric 2. Presentation grading rubric Required proposal and written report format Sample proposal Sample written report 1 2 4 4 4 5 6 7 8 10 12 ASSIGNMENTS: 1. LIST OF QUESTIONS (10 pts): Each student will write down three research questions related to the ecology of the Sonoran Desert that could be answered in two hours of data collection in the desert at the Pima Community College West Campus and turn them in to me. Make sure each question can be answered just by analyzing the data you collect (i.e., without a literature review). Your questions should be descriptive, “Are…?” questions about things that you can easily observe. Do not ask “Why…?” questions!!!! For example, ask, “Are there more holes on one side of a cactus than on the other sides?” Don’t ask, “Why are there more holes on one side of a cactus than on the other sides?” Before you can legitimately ask a “Why” question, you need to answer the “Are” question. (Maybe there aren’t more holes on one side of a cactus than on the other sides.) You will ask the “Why” question and create multiple hypotheses after you answer your descriptive question. Hints for coming up with good, answerable questions: A. Look for aspects of plant or animal ecology that are readily observable, such as plant density (number of individuals per square meter), plant species richness (number of plant species), plant size, locations of rodent burrows or woodpecker holes, positions of cactus arms, etc. B. Then, ask how some of these readily observable variables vary over space or are related to each other. 1 BIO 105, CRN 21684, Spring 2014 – Desert Ecology Lab C. Some of the key things that shape desert ecology and therefore might affect the variables you are interested in are: i. Availability of water: We have a wash on campus. Plants in the wash will receive more water than plants away from the wash, so you could compare aspects of plant or animal ecology in and away from the wash. ii. Amount of sunlight received: The south sides of hills and of plants receive more sunlight than the north sides and therefore tend to be drier and hotter than the north sides. We have a south-facing slope and a north-facing slope on campus, and of course, every plant has a south and a north side, which you could compare. iii. Competition and facilitation between plants: Plants compete fiercely with each other for limiting resources such as water. Some plants even engage in chemical warfare with each other, releasing compounds into the soil that are poisonous to other plants. On the other hand, areas under large shrubs or trees are cooler than areas exposed to direct sunlight and can have more soil moisture as a result. Also, many of the trees and shrubs in the Sonoran Desert are able to convert atmospheric nitrogen to forms that plants can use, so that soil under them can have heightened levels of this important nutrient. Because of these effects, seedlings of other plants may be better able to establish themselves under shrubs or trees than in more exposed locations. iv. Human impacts: Humans alter the environment in a wide range of ways that can affect plants and animals. For instance, areas immediately adjacent to roads tend to receive more water than areas away from roads because of the rainwater that washes off of the roads. These areas also receive more pollution and are avoided by some animals because of traffic noise. There are several roads adjacent to desert around the campus that you could use to look at these effects. Areas near buildings and gardens, such as the classroom buildings on campus, also often receive more water than natural areas because of irrigation and water running off of roofs, sidewalks, etc. These areas will also often receive more pollution as well as trampling and disturbances caused by people walking around. 2. DRAFT PROPOSAL (10 pts): You will choose a partner, and the two of you will develop a research proposal. Before you start: Select which roles each person will have. You will need to collaborate on developing the content of the proposal. However, one person should take the lead in writing the Introduction and Results sections and the other person should take the lead in writing the Methods and Discussion sections. You will need to write these roles on each assignment you turn in and your grade will be based primarily on the sections for which you took the lead (although a component of your grade will be based on the entire project). (See the grading rubrics below.) The final pages of this lab show the correct format for your proposal and an example of a proposal in the required format. You must follow the format I provide exactly! To receive full credit on the draft proposal, you need to have filled in each section (including all the subsections) of the proposal. This draft will give me an opportunity to locate 2 BIO 105, CRN 21684, Spring 2014 – Desert Ecology Lab problems that need to be addressed before you turn in the final proposal. Follow these steps to develop your proposal: A. Select a research question to investigate. This will likely be one of the ones that you wrote down for the first assignment but does not have to be. It does, however, have to be different from every other group’s questions. B. Refine your question and make your (empty) data table. Before you go any further, you need to know exactly what you are asking. That means you need to know what your explanatory and response variables are and how you would measure them. The best way to make sure you have done this is to write down what your data are likely to look like. In other words, create the table you will put your data into and the table and/or graphs you will use to summarize your data. If you make a graph, put the explanatory variable on the x-axis and the response variable on the y-axis. (See the sample proposal for an example.) C. Select a statistical test you can use to analyze your data. Appropriate tests for different types of situations will be covered in class. Doing this step early can prevent you from wasting time on a question that you cannot answer, given the simple statistics that you know. D. Explain why you expect your explanatory variable to influence your response variable. This explanation will form the “Biological Interest” section of your proposal. Make sure you describe the mechanism by which the explanatory variable affects the response variable. You may need to do some library or web research to complete this section. Make sure to provide literature citations for any resources you use. i. Example 1: If you were comparing plant density on south-facing and north-facing slopes, then you should talk about how conditions might differ on the two slopes (e.g., amount of direct sunlight, evaporation rates, etc.) and how these conditions might affect the plant density. ii. Example 2: If you were studying preferences birds have for different kinds of seeds, then you would need to know how the seeds differ (in nutrition, size, difficulty to eat, closeness to natural food, etc.) and why this might cause birds to select one type of seed over the other. E. Come up with hypotheses and predictions. For the kinds of questions we are investigating, there will be a null hypothesis (H0) that states that there is no relationship between the explanatory and response variables and one or two alternative hypotheses (H1 and H2) that state that there is a relationship between the explanatory and response variables (and possibly specify what kind of relationship). For example, your hypotheses could be: i. H0: There are about the same number of holes on the north and south sides of a cactus. ii. H1: There are more holes on the south side of a cactus than on the north side. iii. H2: There are more holes on the north side of a cactus than on the south side. F. Develop your methods. You have already made a start on this task by figuring out what your data are likely to look like. Now, imagine collected those data and 3 BIO 105, CRN 21684, Spring 2014 – Desert Ecology Lab try to think of everything you will need to do. The proposal format provided later in this handout will help you to avoid forgetting important aspects of your methods. Write down your methods in that format. As you design your methods, remember the guidelines for designing scientific studies that I have presented in class! G. Create a proposal title. Make sure your title is descriptive. Usually your best option will be to use your research question as your title. H. Fill in your proposal. Use the sample proposal as a template: I suggest cutting and pasting the sample proposal into a new document and then just changing the text within each section. If you have done all the previous steps, this step should be simple. 3. PROPOSAL (25 pts): Revise your draft proposal until it is as good as you can make it, and then turn it in as the final proposal. Again, you will need to tell me who took the lead in writing the Introduction and Results sections and who took the lead in writing the Methods section. See the proposal and written report grading rubric later in this document for details on how I shall grade your proposal. 4. DATA TABLE (10 pts): Once you have received my comments on your proposal and made any needed changes to your methods, you can collect your data. I shall give you two hours of class time (on two separate days) in which to collect data. Be sure to take some photographs of your study organism and of you doing the measurements! (You’ll want them for your presentation.) After you have collected the data, put it into an Excel workbook in a format that will make it easy to analyze. Make sure to make your column headings clear enough that I can understand what the numbers are, and/or include some explanatory text at the top of the table that tells me what the numbers mean. Then submit the Excel file to me. 5. WRITTEN REPORT (25 pts): Now analyze your data, using the StatisticsFile.xls. Discuss with your partner what the results tell you about your hypotheses. Also talk through all of the limitations your data may have that could influence how you interpret it. You will also identify a new, causal question regarding why you found what you did that could be explored in future work. Then develop several plausible hypotheses that answer this question. You may need to do some library or web research to help you generate your hypotheses. Make sure to provide literature citations for any resources you use. You can convert your proposal into the final, written report by revising the Methods section to reflect what you actually did (and changing it to past tense), re-writing the Results section so that it presents the data you collected, and writing a Discussion section that summarizes your findings, discusses limitations in your data, and presents your new question and hypotheses. Again, to receive full credit, you must follow the format I give at the end of this document exactly. As before, you will need to tell me who took the lead in writing the Introduction and Results sections and who took the lead in writing the Methods and Discussion sections. Keep the same roles as when you wrote your proposal. 4 BIO 105, CRN 21684, Spring 2014 – Desert Ecology Lab See the proposal and written report grading rubric later in this document for details on how I shall grade your written report. 6. PRESENTATION (30 pts): You will prepare a 7-12 minute Powerpoint presentation describing your study and present it to the class. Make sure you follow the Tips for Powerpoint Presentations provided on MyPima! Your presentation will be based on your written report. As before, you need to tell me who was primarily responsible for preparing each section. Use the same roles as in the proposal and written report (one person does the Introduction and Results and the other does the Methods and Discussion). See the presentation grading rubric later in this document for details on how I shall grade your presentation. You must email me your slides no later than 9AM on the day of the presentation, so that I can be sure to get them onto the computer before class begins. Here is what it should contain (in this order): A. Title slide: Title, your name(s), your roles, and relevant picture(s) B. Introduction: i. Research question ii. Biological Interest: 1. Get the audience excited to learn what you found out!! 2. Make sure you present the biology behind your research question in a way that leaves the answer open. 3. Show us pictures of your study subject. C. Methods: Explain how you collected your data, using photographs to illustrate what you did. D. Results: i. Present and explain tables and graphs that summarize your results (the same ones you used in your written report). ii. State whether you found a statistically significant pattern. E. Discussion: i. Tentative conclusion: Tell us the answer to your research question, based on your results. ii. Limitations to results: Tell us about the limitations to your results and tell us what you would do differently if you repeated this study. iii. Causal question [note the spelling!]: Ask why you found what you did. iv. Multiple hypotheses: State at least two hypotheses to answer your causal question and explain why each hypothesis is plausible. 5 BIO 105, CRN 21684, Spring 2014 – Desert Ecology Lab PROPOSAL AND WRITTEN REPORT GRADING RUBRICS Overall quality of the entire proposal or written report To calculate a grade for this section, I shall add together the scores for content for the sections written by you and by your partner and divide by 2 Quality of content presented in your sections EXCELLENT – 10 pts. You include all the material described in the instructions and format guidelines, you demonstrate that you understand the scientific method, your results, and the statistics you used, and you show that you have thought deeply about your research questions and hypotheses and done online/library research as needed to develop your hypotheses GOOD – 8 pts. You include the material listed in the instructions and format guidelines with minor omissions at most, you demonstrate a reasonably good understanding of the scientific method, your results, and the statistics you used and some thought regarding your questions and hypotheses, but there may be some misunderstandings or your thinking may be somewhat shallow POOR – 6 pts. You leave out important material, demonstrate serious misunderstandings of the scientific method, your results, or the statistics you used, or demonstrate little thought regarding your questions and hypotheses VERY POOR – 4 pts. There are major omissions and misunderstandings throughout and little evidence that you have thought about your questions and hypotheses Organization, writing style and grammar in your sections EXCELLENT – 5 pts. : You follow the required format exactly and your writing is easy to understand, with no grammatical or spelling errors GOOD – 4 pts. : You generally follow the required format, but there may be a few deviations from the correct format, some writing that is hard to understand, and/or some grammatical or spelling errors POOR – 3 pts. : You deviate from the required format in significant ways, your writing is difficult to understand, and/or there are many grammatical or spelling errors 10 pts. 10 pts. 5 pts. 6 BIO 105, CRN 21684, Spring 2014 – Desert Ecology Lab PRESENTATION GRADING RUBRIC I shall place your work into the categories that best describe it and assign points accordingly. Overall quality of the entire presentation To calculate a grade for this section, I shall add together the scores for content and use of powerpoint for the sections developed and presented by you and by your partner and divide by 3 Quality of content presented in your sections EXCELLENT – 10 pts. You include all the material listed in the instructions, in the correct order, you demonstrate that you understand the scientific method, your results, and the statistics you used, and you show that you have thought deeply about your research questions and hypotheses and done online/library research as needed to develop your hypotheses GOOD – 8 pts. You include the material listed in the instructions with minor omissions at most, you demonstrate a reasonably good understanding of the scientific method, your results, and the statistics you used and some thought regarding your questions and hypotheses, but there may be some misunderstandings or your thinking may be somewhat shallow POOR – 6 pts. You leave out important material, demonstrate serious misunderstandings of the scientific method, your results, or the statistics you used, or demonstrate little thought regarding your questions and hypotheses VERY POOR – 4 pts. There are major omissions and misunderstandings throughout and little evidence that you have thought about your questions and hypotheses Effective use of powerpoint in your sections EXCELLENT – 5 pts. Your slides are attractive and easy to understand, you use 20+ font size throughout and avoid distracting backgrounds and animations, text is in outline format and is grammatical and spelled correctly, and you illustrate your topic throughout with relevant pictures and diagrams GOOD – 4 pts. Your slides are generally understandable, but you may use too much text at times, sometimes have text that is too small, sometimes include distracting backgrounds or animations, have some grammar or spelling errors, or provide few pictures and diagrams POOR – 3 pts. Your slides are generally difficult to understand, for instance because of confusing wording, too much text, text that is too small, or distracting backgrounds Your presentation style EXCELLENT – 5 pts. You speak to the audience, sound knowledgeable and interested in what you are saying, and speak naturally (without reading slides or notes verbatim) GOOD – 4 pts. You speak clearly and sound knowledgeable but may read some or all of what you present or fail to communicate enthusiasm for the subject POOR – 3 pts. You are difficult to understand and/or sound as though you are unfamiliar with the information you are presenting PENALTY FOR SLIDES NOT PROVIDED ON TIME (BY 9 AM) TOTAL: 10 pts. 10 pts. 5 pts. 5 pts. -3 pts. 30 pts. 7 BIO 105, CRN 21684, Spring 2014 – Desert Ecology Lab REQUIRED PROPOSAL AND WRITTEN REPORT FORMAT This format has been established by the scientific community because of its clarity and organization. Each section must be clearly labeled using the headings and subheadings in bold below. TITLE Your name(s) and roles INTRODUCTION Research Question: State your research question in one sentence with a question mark at the end. Biological Interest: Explain why the explanatory variable might affect the response variable. Make sure you describe the mechanism by which the explanatory variable causes an effect on the dependent variable. Hypotheses and Predictions: State your null hypothesis (H0) and your alternative hypotheses (H1, H2, etc.). (The null hypothesis is the one that states that there is no relationship between the explanatory and response variables.) For each alternative hypothesis, state the statistical criterion you will use to evaluate whether that hypothesis is correct. METHODS [for proposal, this should be in future tense; for written report, this should be in past tense] Dates and Times: Tell the dates and times when you will collect your data (including the year). Must show at least 2 hours of data collection. Location(s): Tell where you will be collecting your data and refer to Figure 1, which should be a map showing the location. (e.g., “We shall collect our data in the wash and adjacent desert areas on the Pima Community College West Campus (Figure 1.)”) Sampling units and sample size: Describe your plots/transects/observation areas, etc., including their size, shape, and how you will place them. Also tell how many plots/transects/observations you expect to use. Data Collection: Tell exactly what data you will collect in each of your sampling units and provide any other details about what you will do that you have not mentioned elsewhere. Data Analysis: Specify how all data are to be summarized (totals, averages, etc.) and analyzed (state which statistics you will use). RESULTS [or, for the proposal, ANTICIPATED RESULTS] Write at least one sentence of text summarizing your data and referring to your table(s) and/or graph(s). For example, “There were more holes on the north than on the south side of cacti (see Table 1 and Figure 2).” (For the proposal: just say, “Table 1 [and Figure 2, etc.] show the kind of data we plan to collect.”) Tables and Figures: Each table and figure (e.g., graph or map) should be named (i.e., Table 1, Table 2, ... Figure 1, Figure 2, ... etc.). Each table and figure should contain a caption that includes enough information to allow the table or figure to stand alone so that someone would understand the data 8 BIO 105, CRN 21684, Spring 2014 – Desert Ecology Lab without reading the rest of the report (e.g., include data units, dates, times, locations, sample size, etc.). (For the proposal: Put in fake data to show what the data might look like: for instance, will your data tables contain counts (e.g., 134 holes on south sides of cacti and 120 holes on north sides) or averages (e.g., 1.2 holes per north side of each cactus, on average, and 0.8 holes per south side of each cactus), if you have graphs, will they be bar graphs or scatter plots, etc. You can leave the pvalues out.) DISCUSSION [For the proposal: skip this section] Summary of results: Write one or two sentences that tell whether you were able to reject your null hypothesis in favor of your alternative hypotheses. If you had more than one alternative hypothesis, tell us which one was supported. Interpretation of results: Write a short paragraph in which you describe what you think your results mean. Make sure to mention the limitations to your data. (All datasets have limitations!) For instance, if you did not find a significant effect, is it possible that there actually is a relationship between the variables that you did not detect because your sample size was too small or because of other problems with your methods? If your results did support one of your alternative hypotheses, are there any reasons why we should be hesitant to embrace that hypothesis? For instance, were there any aspects of your methods that might have biased your results towards that hypothesis? Regardless of what you found, are there any reasons why the data you collected on campus might not apply to the Sonoran Desert in general? Next steps: Tell us a causal question (that is, a “why?” question) regarding why you found what you did. (Usually, that question will simply be “Why was there a relationship between….?” or, “Why did we fail to find a relationship between…?”) Then list at least two hypotheses that answer that question. LITERATURE CITED Alphabetized list of sources fully cited (see How to Cite Sources, available on MyPima). 9 BIO 105, CRN 21684, Spring 2014 – Desert Ecology Lab SAMPLE PROPOSAL (Note: This is made up to illustrate correct format, and its contents are not necessarily accurate.) How does plant density differ between the north-facing and south-facing slope of Tumamoc Hill in Tucson, AZ? Brad Fiero (Introduction and Results) and Carla Essenberg (Methods and Discussion) INTRODUCTION Research Question: How does plant density (succulents, shrubs, and trees combined) differ between the north-facing and south-facing slope of Tumamoc Hill in Tucson, AZ? Biological Interest: South-facing slopes receive more direct sunlight than north-facing slopes, thus will differ in the following respects ... . [State the variables that differ between south-facing and north-facing slopes that could affect plant density. Present relevant plant biology to understand how plant density may be affected by the variables that differ between the two locations]. Hypotheses and Predictions: H0: Plant density will be similar on the north-facing and south-facing slopes of Tumamoc Hill in Tucson, AZ. H1: Plant density will be greater on the north-facing than the south-facing slope of Tumamoc Hill in Tucson, AZ. D1: The p value for the chi square test will be less than 0.05 and the density will be greater on north-facing slope. H2: Plant density will be greater on the south-facing than the north-facing slope of Tumamoc Hill in Tucson, AZ. D2: The p value for the chi square test will be less than 0.05 and the density will be greater on south-facing slope. METHODS Dates and Times: On February 10, 2013 from 12:30-1:30 pm we shall sample the north-facing slope, and on February 15, 2013 from 12:30-1:30 pm we shall sample the south-facing slope. Locations: North and south sides of Tumamoc Hill located on the West side of Tucson, Arizona (Figure 1). Tumamoc Hill is a protected site reaching 947 m (3107 feet) elevation (~200 m above surrounding), and contains natural Sonoran Desert vegetation. 10 BIO 105, CRN 21684, Spring 2014 – Desert Ecology Lab Sampling units and sample size: On the north and south sides of Tumamoc Hill (determined by compass), we shall start at an arbitrary point 100 meters up from the bottom of the hill, and we shall lay out five (more if time allows) 50-meter transects (10 transects total), 20 meters apart, running along the contour of the slope (not up and down the slope). The area sampled will be everything within 10 meters of each transect line (1000 square meters per transect line). Figure 1. Aerial photo of Tumamoc Hill, Tucson, AZ, the location of this study. The approximate locations of the two sampling areas are circled: the one on the north side of the hill is circled in blue, and the one on the south side of the hill is circled in orange. Photo is from maps.google.com. Data Collection: We shall record the number of individuals for all succulents, shrubs, and trees that are at least 10 cm (4") tall within 10 meters of each transect line. Data Analysis: We shall sum the data from the five transects on each side of the hill to calculate density per 5000 square meters. We shall compare densities using the chi square test to determine if there are significant differences (p<0.05) in plant density between the two sides of the hill. ANTICIPATED RESULTS Table 1 shows the kind of data we plan to collect. Table 1. Plant density (number of individuals per 5000 m2) on the north- versus south-facing slope of Tumamoc Hill in Tucson, AZ as observed February 21 and 22, 2012. P-value is from a Chi-square test. Plant Density North-facing Slope 6103 South-facing Slope 5921 p-value LITERATURE CITED MacMahon, J. A. 1985. Deserts. Alfred A. Knopf, New York. 11 BIO 105, CRN 21684, Spring 2014 – Desert Ecology Lab SAMPLE WRITTEN REPORT (NOTE: This is made up just to illustrate correct format. Its contents are not necessarily accurate, and in particular it does not contain real data.) How does plant density differ between the north-facing and south-facing slope of Tumamoc Hill in Tucson, AZ? Brad Fiero (Introduction and Results) and Carla Essenberg (Methods and Discussion) INTRODUCTION Research Question: How does plant density (succulents, shrubs, and trees combined) differ between the north-facing and south-facing slope of Tumamoc Hill in Tucson, AZ? Biological Interest: South-facing slopes receive more direct sunlight than north-facing slopes, thus will differ in the following respects ... . [State the variables that differ between south-facing and north-facing slopes that could affect plant density. Present relevant plant biology to understand how plant density may be affected by the variables that differ between the two locations]. Hypotheses and Predictions: H0: Plant density will be similar on the north-facing and south-facing slopes of Tumamoc Hill in Tucson, AZ. H1: Plant density will be greater on the north-facing than the south-facing slope of Tumamoc Hill in Tucson, AZ. D1: The p value for the chi square test will be less than 0.05 and the density will be greater on north-facing slope. H2: Plant density will be greater on the south-facing than the north-facing slope of Tumamoc Hill in Tucson, AZ. D2: The p value for the chi square test will be less than 0.05 and the density will be greater on south-facing slope. METHODS Dates and Times: On February 10, 2013 from 12:30-1:30 pm we sampled the north-facing slope, and on February 15, 2013 from 12:30-1:30 pm we sampled the south-facing slope. 12 BIO 105, CRN 21684, Spring 2014 – Desert Ecology Lab Locations: North and south sides of Tumamoc Hill located on the West side of Tucson, Arizona (Figure 1). Tumamoc Hill is a protected site reaching 947 m (3107 feet) elevation (~200 m above surrounding), and contains natural Sonoran Desert vegetation. Sampling units and sample size: On the north and south sides of Tumamoc Hill (determined by compass), we started at an arbitrary point 100 meters up from the bottom of the hill and laid out five 50-meter transects (10 transects total), 20 meters apart, running along the contour of the slope (not up and down the slope). The area sampled was be everything within 10 meters of each transect line (1000 square meters per transect line). Figure 1. Aerial photo of Tumamoc Hill, Tucson, AZ, the location of this study. The approximate locations of the two sampling areas are circled: the one on the north side of the hill is circled in blue, and the one on the south side of the hill is circled in orange. Photo is from maps.google.com. Data Collection: We recorded the number of individuals for all succulents, shrubs, and trees that were at least 10 cm (4") tall within 10 meters of each transect line. Data Analysis: We summed the data from the five transects on each side of the hill to calculate density per 5000 square meters. We compared densities using the chi square test to determine if there were significant differences (p<0.05) in plant density between the two sides of the hill. RESULTS We found that plant density was higher on the north side of the slope than the south side (see Table 1). Table 1. Plant density (number of individuals per 5000 m2) on the north- versus south-facing slope of Tumamoc Hill in Tucson, AZ as observed February 21 and 22, 2012. P-value is from a Chi-square test. Plant Density North-facing Slope 5122 South-facing Slope 4807 p-value 0.002 DISCUSSION 13 BIO 105, CRN 21684, Spring 2014 – Desert Ecology Lab Summary of results: Our first alternative hypothesis, that plant density is greater on the north-facing slope than on the south-facing slope of Tumamoc Hill in Tucson, AZ, was supported. Interpretation of results: These results suggest that plant density is higher on the north side than on the south side of Tumamoc Hill. Plant density may be higher on north-facing slopes in general than on south-facing slopes in the Sonoran Desert around Tucson, AZ. However, our study only sampled a single north-facing area and a single south-facing area, and therefore the difference in densities between these two areas results could have been due to many variables other than aspect. For example, the steepness of the slope different between the two sites and the minerals underlying the soil may have been different as well. Samples from many more, separate north-facing and southfacing slopes, which controlled for soil type and steepness, are needed to reach firm conclusions about plant densities on north- vs. south-facing slopes in Sonoran Desert. Next steps: For our next step, we would like to investigate why plant density is higher on the north than on the south side of Tumamoc Hill. Hypotheses to explain that pattern include: 1. The north-facing side retains moisture longer than the south-facing side because it receives less sunlight. 2. The north-facing side retains moisture longer than the south-facing side because its slope is less steep. 3. The north-facing slope has deeper soil because its slope is less steep than the south-facing side, leading to less soil erosion. LITERATURE CITED MacMahon, J. A. 1985. Deserts. Alfred A. Knopf, New York. 14