INTRODUCTION The Bloukrans River is an important river to Grahamstown, it transports the waste produced by the community, and downstream of Grahamstown the farmers use the river for irrigation purposes. As part of an ongoing research, the SASS5 method was used in November 2005 and June 2006 to assess the water quality and the health of the river. METHOD The health of the rivers is determined by assessing the biota in rivers. Biomonitoring was done at the Bloukrans River using SASS5 following the method explained in Dickens CWS and Graham PM (2002). Basic water quality parameters (electrical conductivity, dissolved oxygen, pH and temperature) were measured at each site. SITES SAMPLED All sites surveyed are from the Bloukrans River. Site1 is a section of non-canalized river below small road-bridge near Fort England Hospital. 33˚18’47”S 26˚32’29”E. Stones, vegetation and gravel sand and mud (GSM) were sampled both in November 2005 and June 2006. Site 3 is a section of river below farm road-bridge close to N2 highway bridge. 33˚19’04”S 26˚34’06”E. In November all the biotopes were sampled but in June only the vegetation and GSM were sampled. Site 4 is a section of river below railroad bridge and immediately below road bridge. 33˚19’26”S 26˚35’59”E. Stones, vegetation and GSM were sampled in both seasons. Site 5 is a section of river below a weir on Mr. Duncan’s farm. 33˚19’25”S 26˚38’39”E. Stones, vegetation and GSM were sampled in both seasons. RESULTS Table 1 summarises the SASS5 scores, total number of taxa and ASPT values calculated. The SASS score is simply the sum of the assigned scores of the families encountered in the sample of all the biotopes. The ASPT is calculated by dividing the SASS score by the number of taxa found in the sample. Table1. Summary of SASS5 scores, number of taxa collected and ASPT values at each site along the Bloukrans River. 17 November 2005 and 14 June 2006. Site1 Site3 Site4 Site5 Biotope S VG GSM S VG GSM S VG GSM S VG GSM Sass score Nov 05 9 3 7 39 32 32 26 26 13 20 47 31 No. of taxa Nov 05 4 2 3 10 7 8 7 7 5 6 12 8 No. of taxa Jun 06 4 5 4 9 4 10 8 5 8 7 9 ASPT Nov 05 2.25 1.5 2.33 4 3.7 3.7 2.6 3.3 3.9 3.9 ASPT Jun 06 5.5 3.4 3.5 3.22 3.75 4 3.63 4.2 3.88 5.86 3.56 Sass score Jun 06 22 17 14 29 15 40 29 21 31 41 32 No. of taxa Jun 06 4 5 4 9 4 10 8 5 8 7 9 3.9 4.6 Sass scores stones 60 40 Nov 05 20 Jun 06 0 1 3 4 5 sites Fig.1. Graph representing the SASS5 scores calculated for the stones biotope collected in the Bloukrans River in November 2005 and June 2006. Sass scores vegetation 60 40 Nov 05 20 Jun 06 0 1 3 4 5 sites Fig.2. Graph representing the SASS5 scores calculated for the vegetation biotope collected in the Bloukrans River in November 2005 and June 2006. Sass scores GSM 40 30 20 10 0 Nov 05 Jun 06 1 3 4 5 sites Fig.3. Graph representing the SASS5 scores calculated for the gravel, sand and mud biotope collected in the Bloukrans River in November 2005 and June 2006. No. of taxa stones 15 10 Nov 05 5 Jun 06 0 1 3 4 5 sites Fig.4. Graph representing the number of different invertebrate taxa for the stones biotope collected in the Bloukrans River in November 2005 and June 2006. No. of taxa vegetation 15 10 Nov 05 5 Jun 06 0 1 3 4 5 sites Fig.5. Graph representing the number of different invertebrate taxa for the vegetation biotope collected in the Bloukrans River in November 2005 and June 2006. No. of taxa GSM 10 Nov 05 5 June 06 0 1 3 4 5 sites Fig.6. Graph representing the number of different invertebrate taxa for the gravel, mud and sand biotope collected in the Bloukrans River in November 2005 and June 2006. ASPT scores stones 6 4 Nov 05 2 Jun 06 0 1 3 4 5 sites Fig.7. Graph representing the Average score per taxa (ASPT) calculated for the stones biotope collected in the Bloukrans River in November 2005 and June 2006. ASPT scores vegetation 8 6 4 2 0 Nov 05 Jun 06 1 3 4 5 sites Fig.8. Graph representing the Average score per taxa (ASPT) calculated for the vegetation biotope collected in the Bloukrans River in November 2005 and June 2006. ASPT scores GSM 6 4 Nov 05 2 Jun 06 0 1 3 4 5 sites Fig.9. Graph representing the Average score per taxa (ASPT) calculated for the gravel, sand and mud biotope collected in the Bloukrans River in November 2005 and June 2006. Table 2. Summary of the water chemistry parameters measured in the Bloukrans River in November 2005 Water chemistry Nov 05 Site 1 Site 3 Site 4 Site 5 Temperature (˚C) 20 20 20 19.9 Electrical conductivity (mS/m) 10.36 7.3 9.13 5.8 pH 7.55 7.51 7.45 7.41 Dissolved Oxygen (mg/L) 1.48 7.16 5.23 7.99 Table 3. Summary of the water chemistry parameters measured in the Bloukrans River in June 2006. Water chemistry Jun 06 Site 1 Site 3 Site 4 Site 5 Temperature (˚C) 13 13.4 11 10.4 Electrical conductivity (mS/m) 10.12 10.02 9.85 9.6 pH 8.19 7.29 7.27 7.71 Dissolved Oxygen (mg/L) 9.31 5.2 8.56 8.8 8.5 pH 8 Nov-05 7.5 Jun-06 7 6.5 1 3 4 5 Sites Fig 10. pH in the Bloukrans River in November 2005 and June 2006 at each site. Temperature 25 20 15 Nov-05 10 Jun-06 5 0 1 3 4 5 Sites Fig.11. Temperature in the Bloukrans River in November 2005 and June 2006 at each site. Electrical conductivity (mS/m) 12 10 8 6 4 2 0 Nov-05 Jun-06 1 2 3 4 sites Fig.12 Electrical conductivity in the Bloukrans River in November 2005 and June Dissolved oxygen (mg/L) 2006 at each site. 10 8 6 Nov-05 4 Jun-06 2 0 1 2 3 4 sites Fig.13 Dissolved oxygen in the Bloukraans River in November 2005 and June 2006 at each site. DISCUSSION Based on SASS5 scores obtained the survey conducted in November 2005 reveals that the river was heavily polluted upstream but below the sewage treatment works there is gradual recovery of the river health. In June 2006 site 1 showed an improvement of water quality and deteriorates as you go downstream, but there is an improvement of water quality at site 5. At site 1 the electrical conductivity is high at both the surveys; the introduction of the treated sewage water reduces the conductivity