PROTOCOLS
Complex production
Expression in insect cells: reagents and protocols for multi-protein complex expression using
baculovirus vectors.
J Struct Biol. 2010 Feb 21. [Epub ahead of print] PMID: 2017884
New eukaryotic expression tools for protein complex production.
Trowitzsch, S., Bieniossek, C., Nie, Y., Garzoni, F., Berger, I.
ACEMBL: a versatile and automatable system for protein-complex expression in Escherichia coli.
Nat Methods. 2009 Jun;6(6):447-50. Epub 2009 May 3. PMID: 19412171
Automated unrestricted multigene recombineering for multiprotein complex production.
Bieniossek C, Nie Y, Frey D, Olieric N, Schaffitzel C, Collinson I, Romier C, Berger P, Richmond
TJ, Steinmetz MO, Berger I.
MultiBac: reagents and protocols for multi-protein complex expression using baculovirus vectors.
J Struct Funct Genomics. 2009 Mar;10(1):37-46. Epub 2008 Nov 14. PMID: 19009368
Towards eukaryotic structural complexomics.
Bieniossek C, Berger I.
ESPRIT: automated library-based method for mapping and soluble expression of protein domains
J Struct Biol. 2010 Mar 4. [Epub ahead of print] PMID: 20206698
ESPRIT: An automated, library-based method for mapping and soluble expression of protein
domains from challenging targets
Yumerefendi, H., Tarendeau, F., Mas, P. & Hart, DJ
Mass Spectrometry & Electron Microscopy
Grafix: method that improves sample quality for structure determination by single-particle electron
cryomicroscopy (cryo-EM). GraFix uses a glycerol gradient centrifugation step in which the complexes
are centrifuged into an increasing concentration of a chemical fixation reagent to prevent aggregation
and to stabilize individual macromolecules.
Nat Methods. 2008 Jan;5(1):53-5. Epub 2007 Dec 23. PMID: 18157137
GraFix: sample preparation for single-particle electron cryomicroscopy.
Kastner B, Fischer N, Golas MM, Sander B, Dube P, Boehringer D, Hartmuth K, Deckert J, Hauer
F, Wolf E, Uchtenhagen H, Urlaub H, Herzog F, Peters JM, Poerschke D, Lührmann R, Stark H.
Ion mobility-mass spectrometry approach capable to distinguish the overall subunit architecture of
protein complexes. The approach relies on the simultaneous measurement in the gas phase of the mass
and size of intact assemblies and sub-complexes. These data are then used as restraints to generate
topological models of protein complexes.
Structure. 2009 Sep 9;17(9):1235-43. PMID: 19748344
Subunit architecture of multiprotein assemblies determined using restraints from gas-phase
measurements.
Pukala TL, Ruotolo BT, Zhou M, Politis A, Stefanescu R, Leary JA, Robinson CV.
Nuclear Magnetic Resonance
Protocol for NMR-Based structure determination of Protein Complexes in solution
Angew Chem Int Ed Engl. 2010 Mar 8;49(11):1967-70. PMID: 20148424
An Efficient Protocol for NMR-Based Structure Determination of Protein Complexes in
Solution
Simon B, Madl T, Mackereth CD, Nilges M, Sattler M
A structure refinement protocol combing NMR residual dipolar couplings and small angle
scattering restraints
J Biomol NMR. 2008 Aug;41(4):199-208. Epub 2008 Aug 1.
A structure refinement protocol combining NMR residual dipolar couplings and small angle
scattering restraints.
Gabel F, Simon B, Nilges M, Petoukhov M, Svergun D, Sattler M. PMID: 18670889