Appendix S2. Mass spectrometry analysis of IAA and GAs Prior analysis by LC/MS the samples were derivatised according to the MS-probe concept Kojima et al. (2009). Each sample was dissolved in 75µl of isopropanol and 20µl H2O. Thereafter 4µl 500mM bromocholine (Sigma-Aldrich) in 70% acetonitrile and 0.8µl triethylamine were added to the sample, and kept at 80°C for 2h. After evaporation in a speed-vac concentrator each sample was dissolved in 15-30µl 0.1% HCOOH, and thereafter further analysed by LC/MS in multiple-reaction-monitoring (MRM) mode. Chromatography was performed on a Agilent 1200 HPLC system, equipped with column oven, coupled to a Agilent 6460 triple quadrupole mass spectrometer equipped with a jet stream electrospray source operating in positive ion mode. The jet-stream gas temperature was 200ºC with a gas flow of 6 L hr-1, and a sheath gas temperature equal 400 ºC and flow of 12L/hr; nebulizer pressure was set to 25 psi. The ion spray voltage was set at 3.5 kV for positive ion mode, nozzle voltage was set to 1500V. The fragmentor voltages, transition stages and collision energies were set according to Table S1. N2 was used as collision gas, the dwell time was 100200 ms and the resolution was set to unit resolution. A 5 µL aliquot of sample (4˚C) was injected onto a 2.1 x 50 mm, 1.7 µm HPLC column (Kinetex, Phenomenex) held at 50˚C in a column oven. The gradient elution buffers were A (H2O, 0.1% formic acid) and B (acetonitrile, 0.1% formic acid), and the flow-rate was 300 µl min-1. The mobile phase was first held at 0.9% B for 1 min, and then between 1 and 5 min a linear gradient from 1 to 30% B, between 5 and 12 min from 30 to 60% and then in one min up to 95%B, and held for 1 min. All samples including standards were analysed in randomised order. The LC/MS analysis was done by the MassHunter™ software v 2.00 and data processing by the MassHunter™ Quantitation software v 4.00 (Agilent, USA). Table S1. Summary of retention time and precursor-to-product ion transitions used for the quantification of derivatised IAA and GAs (including internal standards) using LC-ESI-MS/MS. 1 n.d.=not possible to use qualifier ions due to low intensity. Plant hormone RT (min) Fragmentor (V) IAA 13 C6-IAA GA1 2 H2-GA1 GA4 2 H2-GA4 GA8 2 H2-GA8 GA9 2 H2-GA9 4.2 4.2 4.47 4.45 6.20 6.22 3.55 3.53 6.94 6.93 124 124 180 180 185 185 172 172 157 157 Transition stage quantifier 261.1→130.0 267.1→136.0 434.3→375.1 436.3→377.1 418.3→359.1 420.3→361.1 450.3→391.2 452.3→393.2 402.3→343.1 404.3→345.1 Transition stage qualifier 261.1→202.0 267.1→208.0 n.d.1 n.d.1 418.3→225.1 420.3→227.1 n.d.1 n.d.1 402.3→225.1 404.3→277.1 Collision energy quantifier/qualifier (eV) 30/14 30/14 30 30 26/45 26/45 30 30 26/38 26/38 GA19 2 H2-GA19 GA20 2 H2-GA20 GA24 2 H2-GA24 GA29 2 H2-GA29 GA34 2 H2-GA34 GA53 2 H2-GA53 5.36 5.34 5.50 5.49 6.84 6.83 3.80 3.79 4.99 4.99 6.05; 6.40 6.04; 6.38 162 162 155 155 157 157 170 170 155 155 152 152 448.3→389.1 450.3→391.1 418.3→359.1 420.3→361.1 434.3→375.1 436.3→377.1 434.3→375.1 436.3→377.1 434.3→375.1 436.3→377.1 434.3→375.1 436.3→377.1 448.3→299.1 450.3→301.1 418.3→241.1 420.3→243.1 434.3→285.0 436.3→287.1 n.d.1 n.d.1 n.d.1 n.d.1 434.3→285.1 436.3→287.1 22/26 22/26 26/45 26/45 22/30 22/30 26 26 30 30 26/34 26/34