Product Description
ViroReal® Kit Rotavirus (A)
For veterinary use only
ViroReal® Kit Rotavirus (A)
Order no.
Reactions
Pathogen
Internal positive control
DVEV01713
100
FAM channel
Cy5 channel
DVEV01753
50
FAM channel
Cy5 channel
DVEV01711
100
FAM channel
VIC/HEX channel
DVEV01751
50
FAM channel
VIC/HEX channel
Kit content:
 Detection assay for rotavirus (A)
 Detection assay + target for internal RNA positive control
(control of RT-PCR amplification and/or RNA extraction)
 RNA reaction mix
 Nuclease-free water
 Positive control (RNA) for rotavirus
Background: Rotaviruses are the most common cause of dehydrating gastroenteritis in infants and children, and
also in a variety of mammals and birds. Rotavirus is a double-stranded (ds) RNA virus of the family Reoviridae and
is classified into 8 groups (A to H) based on antigenicity and nucleotide sequence identities of the VP6 gene. Group
A causes approximately 90% of infections in humans and animals, group B causes gastroenteritis primarily in adults
and is mainly found in China. Group C is found worldwide but this virus is extremely less prevalent, while group D, E
and F has not been found in humans. The virus is composed of three concentric shells that enclose 11 gene
segments. The outermost shell contains two important proteins – VP7 (G genotypes), and VP4 (P genotypes). The
antigenicity and gene sequence diversity of these proteins define the two serotypes (G-serotype and P-serotype)
and genotypes (G-type and P-type) of the virus. To date, at least 27 G and 37 P genotypes have been identified in
humans and animal species.
Description: ViroReal® Kit Rotavirus (A) is based on the amplification and detection of a part of the non-structural
protein 4 (NSP4), a transmembrane ER-specific glycoprotein, using one-step reverse transcription real-time PCR. It
allows the rapid and sensitive detection of RNA of rotavirus (genotype A) from samples purified from fecal specimen
(e.g. with the QIAamp Viral RNA Mini Kit).
PCR-platforms: ViroReal® Kit Rotavirus (A) is developed and validated for the ABI PRISM® 7500 instrument (Life
Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other realtime PCR instruments.
Specificity: ViroReal® Kit Rotavirus (A) is specific for rotavirus isolates belonging to genotype A.
References: N. Kobayashi, M. Ishino, Y-H. Wang, M. Chawla-Sarkar, T. Krishnan, and T.N. Naik. 2007. Diversity of
G-type and P-type of human an animal rotaviruses and its genetic background. Communicating Current Research
and Educational Topics and Trends in Applied Microbiology: 847 – 858.
ingenetix GmbH
Simmeringer Hauptstr. 24, 1110 Vienna, Austria
T +43 (0)1 74 04 03 91
F +43 (0)1 74 04 03 92
office@ingenetix.com
www.ingenetix.com
ViroReal® Kit Rotavirus (A)
Product description v1.2e
Page 1 of 2
Product Description
Detection of
rotavirus
Detection of
internal RNA positive control IPC-3
Detection of
internal RNA positive control IPC-1
ABI Prism® 7500: FAM channel, 530 nm
ABI Prism® 7500: Cy5 channel, 667 nm
ABI Prism® 7500: VIC channel, 554 nm
1:10 dilution of a rotavirus RNA positive control
Detection of internal RNA positive control
Detection of internal RNA positive control
Mx3005P®: FAM channel
Mx3005P®: CY5 channel
Mx3005P®: HEX channel
1:10 dilution of a rotavirus RNA positive control
Detection of internal RNA positive control
Detection of internal RNA positive control
LightCycler® 480: FAM channel
LightCycler® 480: Cy5 channel
1:10 dilution of a rotavirus RNA positive control
Detection of internal RNA positive control
Ingenetix ViroReal®, BactoReal® and ParoReal Kits run with the same thermal cycling conditions.
RNA and DNA material can be analysed in one PCR run.
For further information on our products please visit our homepage (www.ingenetix.com)
ingenetix GmbH
Simmeringer Hauptstr. 24, 1110 Vienna, Austria
T +43 (0)1 74 04 03 91
F +43 (0)1 74 04 03 92
office@ingenetix.com
www.ingenetix.com
ViroReal® Kit Rotavirus (A)
Product description v1.2e
Page 2 of 2