BIO 150 Study Guide
to accompany Forensic Biology by Richard Li (2008)
Chapter 13: DNA Quantitation
1.
In DNA quantitation, if too much DNA template is used in PCR the result would be:
a. Artifacts which interfere with data analysis and interpretation
b. A partial genotype profile
c. Failure to obtain a profile
d. All of the above
2.
Which of the following is an example of a DNA quantitation method?
a. Interchelating dye
b. Slot blot
c. Quantitative PCR
d. All of the above
3.
Which of the following DNA quantitation methods is the most sensitive?
a. Interchelating dye
b. Slot blot
c. Quantitative PCR
d. RFLP
4.
Choose which of the following quantitative DNA methods can detect any primate DNA but will not detect nonprimate DNA:
a. Interchelating dye
b. Slot blot
c. Gel electrophoresis
d. RFLP
5.
Which of the following DNA quantitation methods can detect PCR inhibitors?
a. Interchelating dye
b. Slot blot
c. STR
d. Quantitative PCR
6.
In this quantitative DNA method, the probe employed is a 40-nucleotide probe complementary to a primate-specific
α satellite DNA sequence at the D17Z1 locus:
a. Interchelating dye
b. Slot blot
c. STR
d. Quantitative PCR
7.
Please choose one of the following that can be a detecting scheme of a slot blot assay:
a. Radioisotope detection
b. STR
c. PCR
d. RFLP
8.
In this DNA quantitation method, a dye is used to stain double-stranded DNA:
a. Interchelating
b. Slot blot
c. STR
d.
9.
Quantitative PCR
This DNA quantitation method is useful when dealing with known reference samples:
a. Interchelating dye
b. Slot blot
c. STR
d. Quantitative PCR
10. There are two categories of quantitative PCR methods, end-point PCR and _______________.
a. PicoGreen
b. dsDNA
c. Real time PCR
d. Slot blot
11. In this category of quantitative PCR methods, a fluorescent reporter is used to monitor the PCR:
a. Real time PCR
b. End-point PCR
c. STR
d. RFLP
12. This quantitative PCR method can detect PCR inhibition:
a. Real time PCR
b. End-point PCR
c. Interchelating Dye
d. RFLP
13. This real-time quantitative PCR method is the most widely used probe technique:
a. TaqMan Method
b. Quant-iT
c. PicoGreen
d. None of the above
14. The cycle threshold in the TaqMan method is defined by:
a. The initial concentration of target sequences
b. The melting temperature
c. The fluorescing level
d. All of the above