ANALYSIS OF THE PERFORMANCE OF THE DEHULLING SYSTEM OF CONFECTIONARY SUNFLOWER GRAINS Ana K. de Figueiredo, Luciana M. Rodríguez, Isabel C. Riccobene, and Susana M. Nolasco Grupo de Investigación TECSE, Facultad de Ingeniería, Universidad Nacional del Centro de la Provincia de Buenos Aires, Av. del Valle 5737, B7400JWI Olavarría, Buenos Aires, Argentina, kdfiguer@fio.unicen.edu.ar, iriccobe@fio.unicen.du.ar, snolasco@fio.unicen.edu.ar ABSTRACT Confectionary sunflower, which has a larger size and lower oil content than oilseed sunflower, has a considerable market, since it is used for birdfeed and human consumption. The grains are classified according to the characteristic dimensions. Medium-size grains are dehulled for use as a snack, a topping on salads, breads, yogurt, and ice cream, or they can be blended into numerous baked goods and confectionary products. Sunflower grains are composed by a pericarp, also known as the “hull” that surrounds the botanic seed, usually called “kernel”. The characteristics of use of confectionary grains require a dehulling process that is efficient in the removal of the hull and that also allows to obtain a product consisting mainly of whole kernels. In the industry, sunflower grains are dehulled by a process based on centrifugal force, where the resulting impact causes the hull to open. The ability of sunflower grains to lose their hull is affected by the moisture content of the grains and by process variables (dehuller rotation speed). This ability can be quantified by means of the “dehulling ability” (DA), the percentage ratio of the percentage of mechanically extracted hull to the total amount of hull. The aim of the present work was to determine an optimal combination of working conditions (impact speed and moisture content of the grain) in the dehulling process of confectionary sunflower grains in order to maximize the DA, maintaining a high percentage of whole kernels (WK, percentage ratio of the mechanically extracted whole kernels to the total amount of kernel of the grains), both expressed on a dry basis (db). The confectionary sunflower hybrid Mycogen 9338 (Morgan) was used. The grains were classified according to size (> 8.75 mm, between 6.50 and 8.75 mm, and < 6.5 mm), carrying out the tests with the intermediate fraction. Grains with different moisture contents were obtained by drying (forced circulation stove at 40-45 ºC) or by spraying a pre-calculated amount of distilled water. Moisture determination was performed by forced-air oven (3 hours, 130 ºC). An experimental design consisting of 11 experiments was used, adding three replicates of the center and four points with star configuration to the full 2 2 factorial design. The impact speeds used (pilot dehulling equipment) were between 2700 and 4000 rpm, and moisture content of the grains was between 4-14% db. The experimental data were adequately fitted into second-order polinomial models (p < 0.0001) that allow to predict the variation of DA and WK with the moisture content of the grain (MC) and its impact speed (IS) within the experimental range studied (lack of fit not significant). The models developed explain more than 82% and 94% of the variation in DA and WK, respectively, based on the selected operating variables (MC and IS). Both response variables (DA and WK) compete with each other, and the improvement of one of them has the opposite effect on the other. The optimization of the system was carried out by using the desirability function (D). The optimal values of the factors were determined by maximizing this function D, using maximum WK and maximum DA as criteria. The results of the optimization technique suggest that by dehulling Mycogen 9338 confectionary sunflower grains (size between 6.50 and 8.75 mm) at 12.3% db and 3100 rpm the maximum values of DA and WK, 72.6% and 63% respectively, can be obtained. The moisture value defined as optimal determines a requirement of humidification of the grain prior to dehulling, establishing the need for a technical and economic feasibility study. The results obtained offer, especially for the oil industry, criteria for the dehulling process of confectionary sunflower grains. This would help to optimize the process, obtain products of better quality and higher profits. Key words: Dehulling, Confectionary sunflower, Whole kernels INTRODUCTION Sunflower is mainly cultivated for commercial oilseed production by pressing and/or solvent extraction. Although the non-oilseed variety (confectionery sunflower, of larger size and lower oil content) is grown to a lesser extent, it presents a wide market because it is used for human consumption and in the food industry for birds and others animals. Confectionary sunflower is generally classified into three categories: the larger grains are roasted, salted and packaged for human consumption; medium size grains are dehulled and packaged for use as snacks or in bakery foods; and the smaller grains are used as poultry feed. Commercial processing of sunflower grains requires achieving an efficient separation of the hull from the grain in the dehulling process. In the oil industry, partial dehulling of the grain presents many advantages, such as a suitable bulk porosity for the oil extraction process, better quality of both raw oil (lower wax content) and de-oiled meal (higher protein content), as well as an increase of the life span of the machinery (Tranchino et al., 1984; Subramanian et al., 1990). On the other hand, regarding confectionary sunflower, the size of the grains and the easiness to remove the hull are two properties that have an essential role in the quality of the obtained product. The characteristics of use of this grain require a dehulling process that is efficient in the removal of the hull and that also allows to obtain a product consisting mainly of whole kernels. The most efficient method for industrial proccessing of sunflower grains is based on a combination of impact and centrifugal forces. During impact dehulling, grains are fed into the top of a spinning rotor, which expels the grains against an impact frame. The force of the impact causes the hull to break away from the kernel, and then the hull is removed by aspiration as the kernels are separated by vibratory sieves. Many investigations have shown that the dehulling ability depends on different characteristics of the grains, such as size and density (Tranchino et al., 1984; Subramanian et al., 1990; Merrien et al., 1992), hull content (Dedio and Dorrell, 1989; Denis et al., 1994), oil content (Beauguillaume and Cadeac, 1992; Merrien et al., 1992) and moisture content (Subramanian et al., 1990; Merrien et al., 1992; Gupta and Das, 1997; Riccobene et al., 2001; de Figueiredo et al., 2011). Subramanian et al. (1990) and Gupta and Das (1999) analyzed the effect of feed rate and the impeller speed on dehulling performance of oilseed sunflower varieties grown in India. Previous studies investigated the effect of moisture content of the grains on the dehulling ability of a non-oilseed sunflower hybrid (de Figueiredo et al., 2011), but the literature does not show studies on confectionary sunflower involving other process variables or a combined study of them. Therefore, the present work was undertaken to determine an optimal combination of the operating conditions (moisture content of the grains and dehuller impact speed) in the dehulling process in order to maximize the dehulling ability maintaining a high percentage of whole kernels. MATHERIALS AND METHODS Sample preparation The confectionary variety Mycogen 9338 (Morgan) was selected for this study. The sunflower grains used were grown Olavarría (36.5º S, 60.5º W), Buenos Aires province, Argentina. The grains were manually cleaned to remove all foreign matter, broken or immature grains. The moisture content of the grains (MC) was determined according to the ASAE S352.2 Method (ASAE, 1999). Both the hull total content (Htotal) and the kernel total content (Ktotal) were determined by manual dehulling from a sample of 10 grams and were expressed as dry basis percentage (%, d.b.). The desired moisture content was obtained by drying the grains in a convection air oven at 40-45 ºC or by spraying with pre-calculated amounts of distilled water, and then thoroughly mixing and sealing them in separate polyethylene bags. The samples were kept in a refrigerator for at least 72 hours to allow a homogeneous moisture distribution. Before starting a test, the required amount of grains was taken out of the refrigerator and allowed to equilibrate to ambient temperature. Dehulling ability and whole kernel percentage The mechanical dehulling was carried out by impact in a dehulling pilot equipment based on a centrifugal process (Figure 1). The rotor speed was adjusted with a variable frequency drive and calibrated with a tachometer. After mechanical dehulling of the sample, the resulting product was classified into hull, whole kernels and rest (undehulled grains, partially dehulled grains, broken kernels and fines, i.e. material smaller than 2 mm). The fractions were dried in a forced-air oven at 130 °C for three hours, and the mechanically-extracted hull/kernel percentages were calculated by equation (1) and (2), respectively, expressed as percentages in dry basis (%, d.b.). Fig. 1. Dehulling pilot equipment. Hmec tota l hull extracted (g) 100 total hull extracted (g) whole kernels extracted (g) rest (g) WK mec whole kernels extracted (g) 100 total hull extracted (g) whole kernels extracted (g) rest (g) (1) (2) The dehulling ability (DA) and the whole kernels production (WK) were determined as the percentage ratio of the percentage of mechanically extracted hull/whole kernels to the total amount of hull/kernels of the grains, equations (3) and (4), respectively. DA (%) H H WK (%) mec 100 (3) total WK K mec 100 (4) total Experimental design and statistical analysis The response surface methodology (RSM) combined with a central composite rotatable design (CCRD) were employed to point out the relationship between the response functions and the process variables, as well as to determine the conditions of these variables able to optimize the performance of the dehulling system for confectionary sunflower grains. The design consisted of 11 experiments with three center points and four axial points added to the 22 full factorial design (Table 1). STATGRAPHICS Centurion XV software (Version 15.2.06, StatPoint, Inc.) was used to perform the statistical analysis of the results, develop multiple regression models from experimental data, and predict process conditions that improve the performance of the dehulling system. Experimental data were fitted to a generalized second order model by the following equation: Yi b0 b1X1 b2X 2 b11X12 b22 X 2 2 b12 X1X 2 (5) where Yi is one of the two predicted responses (dehulling ability of the grains or the percentage of whole kernels) and X1, X2 represent the coded levels of the independent variables. Table 1. Experimental program for the independent variables X1 (moisture content of the grains, d.b.) and X2 (impact speed, rpm) Coded levels Run 1 2 3 4 5 6 7 8 9 10 11 X1 X2 -1 -1 -1 1 1 -1 1 1 -1.41 0 1.41 0 0 -1.41 0 1.41 0 0 0 0 0 0 Actual levels Moisture content (%, d.b.) 6 6 12 12 4.76 13.2 9 9 9 9 9 Impact speed (rpm) 2900 3700 2900 3700 3300 3300 2734 3865 3300 3300 3300 Statistical significance of the terms in the regression equation was examined by analysis of variance (ANOVA) for each response. The optimization of the responses was carried out by using the desirability function (D). The optimal values of the factors (independent variables) were determined by maximizing this function D. A high value of D, from 0 to 1, indicates the best combinations of the factors in order to optimize the studied system (Eren and Kaymak-Ertekin, 2007; Corzo et al., 2008). In the present work, D function was developed for the maximum WK and maximum DA criteria. RESULTS AND DISCUSSION Estimated values of regression coefficients and their corresponding p-values for the DA response are presented in Table 2. They demonstrate that moisture content of the grain (X1) and impact speed (X2) had the largest effect on the DA. These effects were followed by the quadratic term of impact speed (X2). The quadratic term of moisture content (X12) and the interaction between moisture content of the grain and impact speed (X1X2) did not present a significant effect on DA (p > 0.05). Table 2. Regression coefficients of the development model for the dehulling ability bo b1 b2 b11 b22 bi 81.29 -4.49 0.49 -2.97 6.88 p-value 0.001 0.000 0.688 0.026 b12 -0.86 0.555 Table 3 shows the estimated values of regression coefficients and their corresponding p-values for the WK response. All the coefficients were significant (p 0.05), except the interaction moisture content of the grain/impact speed (X1X2). Table 3. Regression coefficients of the development model for the whole kernel percentage bo b1 b2 b11 b22 bi 49.71 12.86 -4.58 -6.90 -13.27 p-value 0.000 0.000 0.006 0.000 b12 0.95 0.577 The prediction models were rearranged by removing the terms that were not significant at 5% in the second order polynomial models, and the corresponding equations are shown in Table 4. Table 4. Adjusted models with only significant coefficients Response DA WK Model equation DA 81.75 4.49X 1 6.88X 2 3.11X 2 2 WK 49.71 12.86X 1 13.27X 2 4.58X 12 6.90X 2 2 Model (p-value) < 0.0001 < 0.0001 0.8282 0.9467 0.7996 0.6308 0.9341 2 R 2 Adjusted R Lack of fit (p-value)b b 0.7643 Want the selected model to have non-significant lack of fit (p > 0.05). ANOVA test revealed that quadratic polinomial models adequately represent the responses of DA and WK with coefficients of determination, R2 = 0.8282 and R2 = 0.9467, respectively (Table 4). R2 and the adjusted R2 values for the studied response variables were equal or higher than 0.80, hence there is a close agreement between the experimental results and the theoretical values predicted by the proposed models. The model adequacy was tested using the lack of fit test which was not significant for p > 0.05. Since the models were found to show insignificant lack of fit (Table 4), the responses were sufficiently explained by the regression equations. The coefficients in Table 4 are presented in terms of coded factors. Thus the size of the coefficients could directly be related to the observed change in the response, enabling a straight comparison between the coefficients. Accordingly, the importance of each factor in the response could be evaluated. The coefficients of moisture content (X1) were, in absolute value, in the same order as those of impact speed (X2), indicating that both factors had the same impact on the considered response. The linear terms of the factors showed an opposite effect on both considered responses. On the other hand, any change in one of the studied factors in order to improve one response has the opposite effect on the other one. Namely, an increase in the impact speed improves the DA of the grains, but reduces the WK percentage. Likewise, an increase in moisture content of the grains in order to achieve a higher WK production reduces the ability of the grains to dehull, (i.e., the DA of the grains). This situation makes difficult the optimization of the dehulling system. In the present work, the optimization procedure was carried out by using the D function. The optimum conditions for dehulling of confectionary grains were determined with the criteria of maximum DA and maximum WK. The regression models developed were used for each response in order to determine the specified optimum conditions. These regression models are valid only in the experimental selected domain, which was determined taking into account some economic and operational considerations of the industry, and quality characteristics of the grains. By applying the desirability function method, the optimal operating conditions, obtained with a desirability value of 0.9460, were 12.3% db for the moisture content of the grains and an impact speed of 3100 rpm for the dehuller equipment. This combination of the factor level would achieve the maximum response values (72.6% and 63.2% for DA and WK, respectively) for Mycogen 9338 confectionary sunflower grains. Considering that confectionary sunflower grains should not be stored with a moisture content above 10-11%, the optimal optimum moisture value obtained from the experimental data (12.3%,d.b.) determines the need to moisturize the grains prior to the dehulling process. Hence, studies of technical and economic feasibility may be necessary. Optimization of the processing operations should be performed in order to obtain the best conditions for the dehulling of grains, resulting in a superior quality product as well as maximizing throughput capacity. 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