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香山生态论坛
XIANGSHAN-ECOLOGY FORUM · CHINA
Serial Reports No. 8
No.2009-8-1
Crop biomass yield - carbon acquisition
and partitioning
Speaker:
Prof. John Patrick
The University of Newcastle, Australia
No.2009-8-1
Transfer cells play a role in seed
development: How are their ingrowth walls
deposited?
Speaker:
Prof. Tina Offler
The University of Newcastle, Australia
Time：2：00 pm Oct. 26, 2009
Venue：Multifunctional conference hall, General Building
（图资楼多功能厅）
Person to contact & Tel：Zhang Wen-Hao (6697)
中国科学院植物研究所植物生态学研究中心
Research Center for Plant Ecology, Institute of Botany, Chinese Academy of Sciences
CURRICULUM VITAE of JOHN. WILLIAM PATRICK
Qualifications:
B.Sc. Agric. (Hons. 1) University of Sydney (1966).
PhD. Macquarie University (1970).
Current Appointment:
Emeritus Professor, Biological Sciences (2007- ).
Previous Academic Appointments:
1973 - Academic staff member, The University of Newcastle.
Lecturer (1973 - 1977);
Senior Lecturer (1978 - 1987);
Associate Professor (1988 – 2000);
Professor, Personal Chair in Biological Sciences (2001- 2007).
Other Visiting and Educational Academic Appointments:
1970 – ’72. Postdoctoral Felllow, University College of Wales, Aberystwyth, Wales, Britain.
1981.
Visiting Senior Research Scientist, CSIRO Plant Industry, Canberra, Australia.
1986.
Visiting Professor, Department of Botany, Washington State University, Pullman, USA.
1994
Principal Research Scientist, HortResearch, Lower Hut, New Zealand.
1998.
Visiting Professor, Department of Botany, Eberhard-Karls-University, Tuebingen, Germany.
2000.
Visiting Professor, ZMBF, Plant Physiology, Eberhard-Karls-University, Tübingen, Germany.
2001.
Visiting Professor, ZMBF, Plant Physiology, Eberhard-Karls-University, Tübingen, Germany.
Professional Activities:
Associate Editor, Functional Plant Biology (2008- )
Contributing Reviewer, Faculty 1000 (2000- )
International Editorial Board, Journal of Experimental Botany (1991- )
Organising Committee for International Phloem Transport Conference (1990- )
Referee for 9 x scientific journals, 4 x granting agencies
International and national working groups and committees such as:
Crop Productivity - Research Imperatives Revisited (1986). Kettering Foundation.
International Phloem Transport Conference Committee (1990 to present).
External Review of CSIRO Sub-program: Photoassimilates, Carbon Partitioning and Energy (1998).
Workshop review – CRC Centre for Legumes in Mediterranean Agriculture (1998).
Pre-review of Department of Plant Sciences, University of Tasmania (2003).
Member of 5 x Scientific Societies
Honorary Secretary Australian Society of Plant Scientists (2004 – 2006)
Oz Reader, Australian Research Council (2000-2003; 2007-)
Member Expert Advisory Panel for Biological Sciences and Biotechnology, Australian Research Council
(2004-2006).
Member Scrutiny Committee, Australian Research Council (2008-)
Member Expert Advisory Panel for Future Fellowships, Australian Research Council (2009- )
Member Advisory Board, Australian Plant Phenomics Facility (2008- )
CSR Scientific Advisor for SugarBooster R & D Program (2004- )
Research Training
1. Current Research Higher Degree Students (with 14 PhD completions to date)
Felicity Andriunas co-supervised (60%) with McCurdy and Offler
Katherine Dibley co-supervised (70%) with Offler
Nigel Fisher co-supervised (20%) with Offler, Mahony and Cole
Hui-Ming Zhang co-supervised (60%) with Offler and McCurdy
2. Current Postdoctoral Fellows (in addition to a previous six)
Dr Emily Grace
Significant Contributions to Research Field
The paradigm that sink processes exert primary control over resource distribution in plants (Patrick 1997) has
guided my approach to discovering how nutrient (and water) transport is regulated. In established
non-meristimatic sinks, control is restricted to sink transport processes (Patrick 1997). To examine these transport
phenomena experimentally, we introduced an innovative model using developing legume seeds (Patrick &
McDonald 1980). Thereafter, substantial progress has been made in understanding cellular pathway, mechanism
and control of nutrient and water import into seed sinks (Zhang et al., 2007). Employing novel structure/function
approaches, CE Offler and I showed that nutrient release/uptake occurs in specialised transport cells located at
maternal/filial seed interfaces (Patrick and Offler 2001). These studies highlighted a central role for transfer cells
in developing seeds (Offler and Patrick 1993). A claim since verified by physiological and molecular observations
(Zhang et al., 2007). With NA Walker, we showed that sucrose release from maternal seed tissues was energy
coupled by a proton antiport mechanism and that uptake by their filial tissues occurs by sucrose/H+ symport
(Patrick 1997). The latter finding was the first direct demonstration of transport function by a higher plant transfer
cell (McDonald et al., 1996 cited in Patrick 1997). These findings underpinned a collaboration with W Frommer
to clone genes encoding sucrose/ (Tegeder et al., 1999) and amino acid/ (Tegeder et al., 2000) H+ symporters and
sucrose effluxers (Zhou et al., 2007a). A molecular genetic approach established that sucrose transporters play a
central role in determining biomass accumulation by seeds (Rosche et al., 2002). With SD Tyerman, we
discovered channels, including novel non-selective channels (Zhang et al., 2004) and aquaporins (Zhou et al.,
2007b), responsible for release of phloem-imported ions and water respectively from seed coats (Zhang et al.
2007). We developed a verifiable model that resource transport in maternal and filial seed tissues is regulated by a
turgor homeostatic mechanism located in maternal seed tissue (Patrick 1997; Zhang et al., 2007). Working along
similar lines to those described above, discovering the cellular pathway of phloem unloading in developing tomato
fruits (Ruan and Patrick 1995) led to identifying hexose transporters as major determinants of fruit sugar levels
(Dibley et al unpubl).
Competitive External Grant Funding (from 2004 only)
CI
Project Title
Patrick, McCurdy & Offler
Induction
of
transfer
cells
-
Year(s)
Granting
Body
Amount
$ (x K)
2006-08
ARC
322
Discovering regulatory networks
Patrick
Phloem unloading of sucrose:
cloning,
characterization
&
regulation of novel membrane
transporters
2003-05
ARC
205
Tyerman & Patrick
Multifunctional channels as key
components of biotrophic interfaces
in legumes
2003-05
ARC
270
Patrick & McCurdy
Genetic engineering of enhanced
sweetness and flavour in tomato
2000-04
ARC
93
Kisi, Donne, Keast, Brichta,
Melchers,
Offler,
O'Connor,
Moheimani, Patrick, van Helden,
King, Evans, Dunstan, Macfarlane,
Ruan, Roselli, Lin, Grof, Frisia,
Lim, Sugo, Forrester, Buzzi,
Fleming, Wanless, Holdsworth,
Drysdale, Fityus, McCurdy, Zhu
Electron
Microscopes
for
Nanometer-scale
Imaging/Microanalysis
in
the
Materials, Biological, Physical,
Engineering and Chemical Sciences.
2009
ARC
LIEF
650
Jones, McLaughlin, Aitken, Rose,
Patrick, Offler, McCurdy, Ashman,
Burns, van Helden, Verrills, Nixon,
Roman, Ruan, Thorne, Calford
Confocal
Laser
Scanning
Microscopy for Live Cell Imaging.
2009
ARC
LIEF
275
Aitken,
McLaughlin,
Lewis,
Griffith, Rose, Patrick, McCurdy,
McCluskey, Von Nagy-Felsobuki,
Dunkley, Dickson, Rostas, Ashman,
Burns, Foster, Gibson, Smith, Sim,
Tooney, Ross
High throughput proteomics Thermo Finnigan ProteomeX LCQ
Integrated Proteomicis Workstation
2005
ARC
LIEF
207
Patrick, Rose, Offler, McCurdy,
Cole, Macfarlane, Rodger
Upgrade and expansion of Newcastle
Plant Growth Facility
2004
ARC
LIEF
329
Ten Best Publications
Patrick JW, McDonald R. (1980). Pathway of carbon transport within developing ovules of Phaseolus vulgaris L.
Australian Journal of Plant Physiology 7, 671-684.
Based on an analysis of unloading pathways, demonstrated utility of excised seed coat cups to study
mechanisms of phloem unloading.
Citation: 55;*JIF: 2.38
Offler CE, Patrick JW. (1993). Pathway of photosynthate transfer in the developing seed of Vicia faba L. A
structural assessment of the role of transfer cells in unloading from the seed coat. Journal of Experimental
Botany 44, 711 - 724.
First demonstration of the putative role of a higher plant transfer cells in transporting nutrients between maternal
and filial tissues of a developing seed.
Citation: 57; JIF: 3.92
Ruan Y-L, Patrick JW. (1995). The cellular pathway of postphloem sugar transport in developing tomato fruit.
Planta 196, 434 - 444.
Discovery of a switch between a symplamic to an apoplasmic pathway of sugar transport in developing
tomato fruit.
Citation: 70; JIF: 3.08
*Patrick JW. (1997). Phloem unloading: Sieve element unloading and post-sieve element transport. Annual
Review of Plant Physiology & Plant Molecular Biology 48, 191 - 222.
Establishes central role of phloem unloading in partitioning of nutrients
Citation:139;JIF: 18.71
*Patrick JW, Offler CE. (2001). Compartmentation of transport and transfer events in developing seeds.
Journal of Experimental Botany 52, 551-564.
Major review on nutrient loading of developing seeds
Citation: 57; JIF: 3.92
Tegeder M, Wang X-D, Frommer WB, Offler CE, Patrick JW. (1999). Sucrose transport into developing seeds of
Pisum sativum L. Plant Journal 18, 151-161.
Cloning of the first transfer cell sucrose transporter.
Citation: 60; JIF: 6.75
Tegeder M, Offler CE, Frommer WB, Patrick JW. (2000). Amino acid transporters are localized to transfer cells
of developing pea seeds. Plant Physiology 122, 319-326.
Cloning of transfer cell amino acid transporters.
Citation: 44; JIF: 6.37
Zhang W-H, Skerrett M, Walker NA, Patrick JW, Tyerman SD. (2002). Non-selective channels in plasma
membranes of protoplasts from coats of developing seeds of bean. Plant Physiology 128, 388-399.
Novel role of a non-selective channel function in unloading nutrients.
Citation: 15; JIF: 6.37
Rosche E, Blackmore D, Tegeder M, Richardson T, Schroeder H, Higgins TJV, Frommer WB, Offler CE, Patrick
JW. (2002). Seed-specific expression of a potato sucrose transporter increases sucrose uptake and growth rates
of developing pea cotyledons. Plant Journal 30, 165-175.
First demonstration of key role sucrose transporters play in sucrose and biomass accumulation by developing
seeds.
Citation: 21; JIF: 6.75
*Zhou Y, Qu H, Dibley KE, Offler CE, Patrick JW. (2007). A suite of sucrose transporters expressed in coats of
developing legume seeds includes novel pH-independent facilitators. Plant Journal 49, 750-764.
First reported cloning and functional characterisation of sucrose facilitators
Citation: 8; JIF: 6.75
* JIF, Journal Impact Factor (current)
PUBLICATION LIST
Summary. 105 Refereed journal articles; 18 Reviews; 20 Book chapters; Over 100 Conference papers including 30
keynote presentations; 4 technical reports.
Refereed Journal Articles
1. Patrick, J. W. (1972). Vascular system of the stem of the wheat plant. I. Mature state. Australian
Journal of Botany 20, 49 - 63.
2. Patrick, J. W. (1972). Vascular system of the stem of the wheat plant. II. Development. Australian
Journal of Botany 20, 65 - 78.
3. Patrick, J. W. (1972). Distribution of assimilate during stem elongation in wheat. Australian Journal of
Biological Sciences 25, 455 - 467.
4. Patrick, J. W. and Wareing, P. F. (1973). Auxin-promoted transport of metabolites in stems of Phaseolus
vulgaris L. Some characteristics of the experimental transport system. Journal of Experimental Botany.
24, 1158 - 1171.
5. Patrick, J. W. and Woolley, D. J. (1973). Auxin physiology of decapitated stems of Phaseoulus vulgaris L.
treated with indol-3yl-acetic acid. Journal of Experimental Botany 24, 949 - 957.
6. El - Beltagy, A. S., Patrick, J. W., Hewett, E. W. and Hall, M. A. (1976). Endogenous plant growth regulator
levels in tomato fruits during development. Journal of Horticulture Science. 51, 15 - 30.
7. Patrick, J. W. and Wareing, P. F. (1976). Auxin-promoted transport of metabolites in stems of Phaseolus
vulgaris L. Effects at the site of hormone application. Journal of Experimental Botany. 27, 969 982.
8. Patrick, J. W. and Wareing, P. F. (1978). Auxin-promoted transport of metabolites in stems of Phaseolus
vulgaris L. Effects remote from the site of hormone application. Journal of Experimental Botany 29,
359 - 366.
9. Mulligan, D. R. and Patrick, J. W. (1979). Gibberellic-acid-promoted transport of assimilates in stems of
Phaseolus vulgaris L. Localised versus remote site(s) of action. Planta 145, 233 - 238.
10. Patrick, J. W. (1979a). Auxin-promoted transport of metabolites in stems of Phaseolus vulgaris L.
Further studies on effects remote from the site of hormone application. Journal of Experimental Botany.
30, 1 - 13.
11. Patrick, J. W. (1979b). An assessment of auxin-promoted transport in decapitated stems and whole shoots
of Phaseolus vulgaris L. Planta 146, 107 - 112.
12. Patrick, J. W., Johnstone, G. F. S. and Wareing, P. F. (1979). Mobilizing ability of gibberellic acid and kinetin
applied to mature, decapitated stems of Phaseolus vulgaris L. Annals of Botany 44, 517 - 519.
13. Turvey, P. M. and Patrick, J. W. (1979). Kinetin-promoted transport of assimilate in stems of Phaseolus
vulgaris L. Localised versus remote site(s) of action. Planta 147, 151 - 155.
14. Patrick, J. W. and McDonald, R. (1980). Pathway of carbon transport within developing ovules of Phaseolus
vulgaris L. Australian Journal of Plant Physiology 7, 671-684.
15. Patrick, J. W. (1981). An in vitro assay of sucrose uptake by developing bean cotyledons. Australian
Journal of Plant Physiology 8, 221-235.
16. Patrick, J. W. and Turvey, P. M. (1981). The pathway of radial transfer of photosynthate in decapitated stems
of Phaseolus vulgaris L. Annals of Botany 47, 611 - 621.
17. King, R. W. and Patrick, J. W. (1982). Control of assimilate movement in wheat. Is abscisic acid involved?
Zeitschrift fur Pflanzenphysiologie 106, 375 - 380.
18. Offler, C. E., Patrick, J. W. and Rose, R. J. (1983). Chloroplast characteristics and efficiency of low
irradiance grown Eucalyptus pilularis Sm. seedlings. Photosynthetica 17, 227 -234.
19. Patrick, J. W. (1983). Photosynthate unloading from seed coats of Phaseolus vulgaris L. General
characteristics and facilitated transfer. Zeitschrift fur Pflanzenphysiologie 111, 9-18.
20. Offler, C. E. and Patrick, J. W. (1984). Cellular structures, plasma membrane surface areas and
plasmodesmatal frequencies of seed coats of Phaseolus vulgaris L. in relation to photosynthate transfer.
Australian Journal of Plant Physiology 11, 79-99.
21. Patrick, J. W. (1984). Photosynthate unloading from seed coats of Phaseolus vulgaris L. Control by
tissue water relations. Journal of Plant Physiology 115, 297-310.
22. Patrick, J. W. and Wardlaw, I. F. (1984). Vascular control of photosynthate transfer from the flag leaf to the
ear of wheat. Australian Journal of Plant Physiology 11, 235 - 241.
23. van Bel, A. J. E. and Patrick, J. W. (1984). No direct linkage between proton pumping and photosynthate
unloading from seed coats of Phaseolus vulgaris L. Plant Growth Regulation 2, 319-326.
24. Hayes, P. M., Offler, C. E. and Patrick, J. W. (1985). Cellular structures, plasma membrane surface areas and
plasmodesmatal frequencies of the stem of Phaseolus vulgaris L. in relation to radial photosynthate
transfer. Annals of Botany 56, 125 - 138.
25. Hayes, P. M. and Patrick, J. W. (1985). Photosynthate transport in stems of Phaseolus vulgaris L. treated
with gibberellic acid, indole-acetic acid or kinetin. Effects at the site of hormone application. Planta
106, 371-379.
26. Heinrich, P. A. and Patrick, J. W. (1985). Phosphorus acquisition in the soil-root system of Eucalyptus
pilularis Sm. l. Characteristics of the soil system. Australian Journal of Soil Science 23, 223 - 236.
27. Mulligan, D. R. and Patrick, J. W. (1985). Phosphorus and carbon economies of ectomycorrhizal seedlings
of Eucalyptus pilularis Smith. Australian Journal of Plant Physiology 12, 669 - 679.
28. Mulligan, D. R. and Patrick, J. W. (1985). Growth of and phosphorus partitioning in Eucalyptus pilularis
Smith seedlings raised in a phosphorus-deficient soil. Australian Journal of Botany 33, 245 - 259.
29. Mulligan, D. R. and Patrick, J. W. (1985). Carbon and phosphorus assimilation and deployment in
Eucalyptus pilularis Smith seedlings with special reference to the role of the cotyledons. Australian
Journal of Botany 33, 485 - 496.
30. Mulligan, D. R. and Patrick, J. W. (1985). Phosphorus and carbon budgets of Eucalyptus pilularis Smith
seedlings during cotyledonary phase of growth. Australian Journal of Plant Physiology 12, 535 - 548.
31. van Bel, A. J. E. and Patrick, J. W. (1985). Proton extrusion in the seed coats of Phaseolus vulgaris L. Plant,
Cell and Environment 8, 1 - 6.
32. Clifford, P. E., Offler, C. E. and Patrick, J. W. (1986). Growth regulators have rapid effects on photosynthate
unloading from seed coats of Phaseolus vulgaris L. Plant Physiology 80, 635-637.
33. Heinrich, P. A. and Patrick, J. W. (1986). Phosphorus acquisition in the soil-root system of Eucalyptus
pilularis Smith seedlings. ll. The effect of ectomycorrhizas on seedling phosphorus and dry weight
acquisition. Australian Journal of Botany 34, 445 - 454.
34. Patrick, J. W., Jacobs, E., Offler, C. E. and Cram, W. J. (1986). Photosynthate unloading from seed coats
of Phaseolus vulgaris L. - Nature and cellular location of turgor-sensitive unloading. Journal of
Experimental Botany 37, 1006-1019.
35. Clifford, P. E., Offler, C. E. and Patrick, J. W. (1987). Injection of growth regulators into seeds growing in
situ on plants of Phaseolus vulgaris L. with a double-fruit sink system. Canadian Journal of Botany.
65, 612 - 615.
36. Hayes, P. M., Patrick, J. W. and Offler, C. E. (1987). The cellular pathway of radial transfer of
photosynthates in stems of Phaseolus vulgaris L.: Effect of cellular plasmolysis and
p-chloromercuribenzene sulfonic acid. Annals of Botany 59, 635 - 642.
37. Patrick, J. W. (1987). Effect of potassium on photosynthate unloading from seed coats of Phaseolus vulgaris
L. Specificity and membrane location. Annals of Botany 59, 181 - 190.
38. Patrick, J. W. and Steains, K. H. (1987). Auxin-promoted transport of metabolites in stems of Phaseolus
vulgaris L. Auxin dose-response curves and effects of inhibitors of polar auxin transport. Journal of
Experimental Botany 38, 203 - 210.
39. Heinrich, P. A., Mulligan, D. R. and Patrick, J. W. (1988). The effect of ectomycorrhizas on the phosphorus
and dry weight acquisition of Eucalyptus seedlings. Plant and Soil 109, 147 - 149.
40. Offler, C. E., Nerlich, S. M. and Patrick, J. W. (1989). Pathway of photosynthate transfer in the developing
seed of Vicia faba L. Transfer in relation to seed anatomy. Journal of Experimental Botany 40,
769-780.
41. Patrick, J. W. and Mulligan, D. R. (1989). Gibberellic-acid-promoted transport of assimilates in stems of
Phaseolus vulgaris L. Effects on assimilate accumulation by the sink. Annals of Botany 63, 581 589.
42. Clifford, P. E., Offler, C. E. and Patrick, J. W. (1989). How are sugars unloaded from the phloem? Some
answers from a novel experimental system. Journal of Biological Education 23, 147 – 151.
43. Shen, Y.-W. and Patrick, J. W. (1990). Cellular pathway and hormonal promotion of phloem unloading in
stems of Phaseolus vulgaris L. Acta Phytophysiology Sinica 16, 115 - 124.
44. Shen, Y.-W. and Patrick, J. W. (1990). Photosynthate unloading from seed coats of Phaseolus vulgaris L. An
attempt to define conditions required for stimulation by abscisic acid and benzylamino purine. Acta
Agriculture Universitatis Zhajiangensis. 16, 454 - 462.
45. Fieuw, S. and Patrick, J. W. (1993). Mechanism of photosynthate efflux from Vicia faba L. seed coats. I.
Tissue studies. Journal of Experimental Botany 44, 65 - 74.
46. Offler, C. E. and Patrick, J. W. (1993). Pathway of photosynthate transfer in the developing seed of Vicia
faba L. A structural assessment of the role of transfer cells in unloading from the seed coat. Journal of
Experimental Botany 44, 711 - 724.
47. Patrick, J. W. (1993a). Osmotic regulation of assimilate unloading from seed coats of Vicia faba L.
Assimilate partitioning to and within attached seed coats. Physiologia Plantarum 87, 345 - 352.
48. Patrick, J. W. (1993b). Osmotic regulation of assimilate unloading from seed coats of Vicia faba. Role of
turgor and identification of turgor-dependent fluxes. Physiologia Plantarum 89, 87 - 96.
49. Wang, H. L., Patrick, J. W., Offler, C. E. and Wardlaw, I. F. (1993). A novel experimental system for studies
of photosynthate transfer in the developing wheat grain. Journal of Experimental Botany 44, 1177 1184.
50. Patrick, J. W. (1994a). Turgor-dependent unloading of photosynthates from coats of developing seed of
Phaseolus vulgaris and Vicia faba. Turgor homeostasis and set points. Physiologia Plantarum 90,
367 - 377.
51. Patrick, J. W. (1994b). Turgor-dependent unloading of assimilates from coats of developing legume seed.
Assessment of the significance of the phenomenon in the whole plant. Physiologia Plantarum 90, 645
- 654.
52. Wang, H. L., Offler, C. E., Patrick, J. W. and Ugalde, T. D. (1994). The cellular pathway of photosynthate
transfer in the developing wheat grain. I. Delineation of a potential transfer pathway using fluorescent
dyes. Plant, Cell & Environment 17, 257 - 266.
53. Wang, H. L., Offler, C. E. and Patrick, J. W. (1994). Nucellar projection transfer cells in the developing
wheat grain. Protoplasma 182, 39 -52.
54. Wood, R. M., Patrick, J. W. and Offler, C. E. (1994). The cellular pathway of short-distance transfer of
photosynthates and potassium in the elongating stem of Phaseolus vulgaris L. Stem anatomy, solute
transport and pool sizes. Annals of Botany 73, 151 - 160.
55. Patrick, J. W., Offler, C. E. and Wang, X-D. (1995). Cellular pathway of photosynthate transport in coats of
developing seed of Vicia faba L. and Phaseolus vulgaris L. I. Extent of transport through the coat
symplast. Journal of Experimental Botany 46, 35 - 47.
56. McDonald, R., Wang, H. L., Patrick, J. W. and Offler, C. E. (1995). Cellular pathway of sucrose transport in
developing cotyledons of Vicia faba L. and Phaseolus vulgaris L. A physiological assessment. Planta
196, 659 - 667.
57. Ruan, Y-L., Mate, C., Patrick, J. W. and Brady, C. J. (1995). Non-destructive collection of apoplast fluid
from developing tomato fruit using a pressure dehydration procedure. Australian Journal of Plant
Physiology 22, 761 - 769.
58. Ruan, Y-L. and Patrick, J. W. (1995). The cellular pathway of post-phloem sugar transport in developing
tomato fruit. Planta 196, 434 - 444.
59. Walker, N. A., Patrick, J. W., Zhang, W. and Fieuw, S. (1995). Mechanism of photosynthate efflux from
seed coats of Phaseolus vulgaris: a chemiosmotic analysis. Journal of Experimental Botany 46, 539 549.
60. Wang, X-D., Harrington, G., Patrick, J. W., Offler, C. E. and Fieuw, S. (1995). Cellular pathway of
photosynthate transport in coats of developing seed of Vicia faba L. and Phaseolus vulgaris L. II.
Principal cellular site(s) of efflux. Journal of Experimental Botany 46, 49 -63.
61. Wang, H. L., Offler, C. E. and Patrick, J. W. (1995). Cellular pathway of photosynthate transfer in the
developing wheat grain. II. A structural analysis and histochemical studies of the transfer pathway from
the crease phloem to the endosperm cavity. Plant, Cell and Environment 18, 373 - 388.
62. Wang, H. L., Patrick, J. W. and Offler, C. E. (1995). Cellular pathway of photosynthate transfer in the
developing wheat grain. III. A structural analysis and physiological studies of the pathway from the
endosperm cavity to the starchy endosperm. Plant, Cell and Environment 18, 389 - 407.
63. Wardlaw, I. F., Moncur, L. and Patrick, J. W. (1995). The response of wheat to high temperature following
anthesis: Sucrose accumulation and metabolism by isolated kernels. Australian Journal of Plant
Physiology 22, 399 - 407.
64. McDonald, R., Fieuw, S. and Patrick, J. W. (1996a). Sugar uptake by the dermal transfer cells of developing
cotyledons of Vicia faba L. 1. Experimental systems and general transport properties. Planta 198,
54 - 63.
65. McDonald, R., Fieuw, S. and Patrick, J. W. (1996b). Sugar uptake by the dermal transfer cells of
developing cotyledons of Vicia faba L. II. Mechanism of energy coupling. Planta 198, 502 - 509.
66. Ruan, Y-L., Brady, C. J. and Patrick, J. W. (1996). The composition of apoplast fluid recovered from intact
developing tomato fruit. Australian Journal of Plant Physiology 23, 9 - 13.
67. Zhang, W., Atwell, B. J., Patrick, J. W. and Walker, N. A. (1996). Turgor-dependent efflux of assimilates
from coats of developing seed of Phaseolus vulgaris L. Water relations of the efflux cells. Planta 199, 25
- 33.
68. Harrington, G. N., Franceschi, V. R., Offler, C. E., Patrick, J. W. Tegeder, M., Frommer, W. B., Harper, J. F. &
Hitz, W. D. (1997). Cell specific expression of three genes involved in plasma membrane sucrose
transport in developing Vicia faba seed. Protoplasma 197, 160-173.
69. Harrington, G. N., Nussbaumer, Y., Wang, X-D., Tegeder, M., Franceschi, V. R., Frommer, W. B., Patrick, J. W.
and Offler, C. E. (1997b) Spatial and temporal expression of sucrose transport-related genes in
developing cotyledons of Vicia faba L. Protoplasma 200, 35 - 50.
70. Ruan, Y-L., Patrick, J. W. and Brady, C.J. (1997). Protoplast hexose carrier activity is a determinate of
genotypic difference in hexose storage in tomato fruit. Plant Cell and Environment 20, 341 - 349.
71. Wood, R. M., Offler, C. E. and Patrick, J. W. (1997). The cellular pathway of short-distance transfer of
photosynthate and potassium in the elongating stem of Phaseolus vulgaris L. A structural assessment.
Annals of Botany 79, 89 - 100.
72. Zhang, W. H., Walker, N. A., Tyerman, S. D. and Patrick, J. W. (1997). Mechanisms of solute efflux from
seed coats: Whole-cell K+currents in transfer cell protoplasts derived from coats of developing seeds of
Vicia faba L. Journal of Experimental Botany 48, 1565-1572.
73. Darussalam, Cole, M. A. and Patrick, J. W. (1998). Auxin control of photoassimilate transport to and within
developing grains of wheat Australian Journal of Plant Physiology 25, 69-77.
74. Wood, R. M., Patrick, J. W., and Offler, C. E. (1998). The cellular pathway of short-distance transfer of
photosynthates and potassium in the elongating stem of Phaseolus vulgaris L. A physiological
assessment. Annals of Botany 82, 337 - 345.
75. Tegeder, M., Wang, X-D., Frommer, W. B., Offler, C. E. and Patrick, J. W. (1999). Sucrose transport
into developing seeds of Pisum sativum L. Plant Journal 18, 151-161.
76. Bagnall, N., Wang, X-D., Scofield, G. N., Furbank, R. T., Offler, C. E. and Patrick, J. W. (2000). Sucrose
transport-related genes are expressed in both maternal and filial tissues of developing wheat grains.
Australian Journal of Plant Physiology 27, 1009-1020.
77. Farley, S. J., Patrick, J. W. and Offler, C. E. (2000). Transfer cell differentiate in cultured cotyledons of Vicia
faba L. seeds. Protoplasma 214, 102-117.
78. Gear, M., McPhillips, M., Patrick, J. W. and McCurdy, D. W. (2000). Hexose transporters of tomato:
molecular cloning, expression analysis and functional characterization. Plant Molecular Biology 44,
687-697.
79. Tegeder, M., Offler, C.E., Frommer, W.B. and Patrick, J.W. (2000a) Amino acid transporters are localized to
transfer cells of developing pea seeds. Plant Physiology 122, 319-326.
80. Tegeder, M., Thomas, M., Hetherington, L., Wang, X.-D., Offler, C.E. and Patrick, J.W. (2000b)
Genotypic differences in seed growth rates of Phaseolus vulgaris L. Factors contributing to cotyledon sink
activity and sink size. Australian Journal of Plant Physiology 27, 119-128.
81. Thomas, M., Hetherington, L. and Patrick, J.W. (2000). Genotypic differences in seed growth rates of
Phaseolus vulgaris L. General characteristics, seed coat factors and comparative roles of seed coats and
cotyledons. Australian Journal of Plant Physiology 27, 109-118.
82. Walker, N. A., Zhang, W-H., Harrington, G., Holdaway, N. and Patrick, J.W. (2000). Effluxes of solutes
from developing coats of Phaseolus vulgaris L. & Vicia faba L.: Locating the effects of turgor in a
coupled chemiosomotic system. Journal of Experimental Botany 51, 1047-1055.
83. Zhang, W-H., Walker, N. A., Tyerman, S. D. and Patrick, J. W. (2000). Fast-activation of a time-dependent
outward current in protoplasts derived from coats of developing Phaseolus vulgaris seeds. Planta 211,
894-898.
84. Furbank, R. T., Scofield, G., Hirose, T., Wang, X-D., Patrick, J. W. and Offler, C. E. (2001). Cellular
localisation and function of a sucrose transporter OsSUT1 in developing rice grains. Australian Journal
of Plant Physiology. 29, 1187-1196.
85. Aoki, N., Whitfield, P., Hoeren, F., Scofield, G., Newell, K., Patrick, J., Offler, C., Clarke, B., Rahman, S. and
Furbank, R. T. (2002). Three sucrose transporter genes are expressed in the developing grain of
hexaploid wheat. Plant Molecular Biology 50, 453-462.
86. Rosche, E., Blackmore, D., Tegeder, M., Richardson, T., Schroeder, H., Higgins, T. J. V., Frommer, W. B.,
Offler, C. E. and Patrick, J. W., (2002). Seed-specific expression of a potato sucrose transporter
increases sucrose uptake and growth rates of developing pea cotyledons. Plant Journal 30, 165-175.
87. Zhang, W-H., Skerrett, M., Walker, N.A., Patrick, J. W. and Tyerman, S. D. (2002). Non-selective channels
in plasma membranes of protoplasts from coats of developing seeds of bean. Plant Physiology 128,
1187-1196.
88. Ritchie, R., Fieuw-Makeroff, S. and Patrick, J. W. (2003). Sugar retrieval by coats of developing seeds of
Phaseolus vulgaris L. and Vicia faba L. Plant Cell and Physiology 42, 163-172.
89. Aoki N, Scofield GN, Wang X-D, Patrick JW, Offler CE, Furbank RT (2004). Expression and localisation of
the wheat sucrose transporter TaSUT1 in vegetative tissues. Planta 219, 176-184.
90. Zhang W-H, Walker NA, Patrick JW, Tyerman SD (2004) Pulsing Cl- channels in coat cells of developing
bean seeds linked to hypo-osmotic turgor regulation. Journal of Experimental Botany 55, 993-1001.
91. Zhang W-H, Walker NA, Patrick JW, Tyerman SD (2004). Calcium - dependent K current in plasma
membranes of dermal cells of developing bean cotyledons. Plant, Cell & Environment 27, 251-262.
92. Harrington, GN, Dibley KE, Ritchie RJ, Offler CE, Patrick JW (2005). Hexose uptake by developing
cotyledons of Vicia faba: physiological evidence for transporters of differing affinities and specificities.
Functional Plant Biology 32, 987-995.
93. Rosche EG, Blackmore D, Offler CE, Patrick JW (2005). Increased capacity fro sucrose uptake leads to earlier
onset of protein accumulation in developing pea seeds. Functional Plant Biology 32, 997-1007.
94. Dibley SJ, Gear ML, Yang X, Rosche EG, Offler CE, McCurdy DW, Patrick JW (2005). Temporal and spatial
expression of hexose transporters in developing tomato (Lycopersicon esculentum) fruit. Functional Plant
Biology 32, 777-785
95. Aoki N, Scofield GN, Wang XD, Offler CE, Patrick JW, Furbank RT (2006). Pathway of sugar transport in
germinating wheat seeds Plant Physiology 141, 1255-126
96. Koller CE, Patrick JW, Rose RJ, Offler CE, MacFarlane GR (i2007). Pteris umbrosa R. Br as an arsenic
hyperaccumulator: Accumulation, partitioning and comparison with the established As hyperaccumulator
Pteris vittata. Chemosphere 66, 1256-1263.
97. Wardini T, Talbot MJ, Offler CE, Patrick JW (2007). Role of sugars in regulating transfer cell development
in cotyledons of Vicia faba seeds. Protoplasma 230, 75-88.
98. Wardini T, Wang X-D, Offler CE, Patrick JW (2007). Induction of wall ingrowths of transfer cells occurs
rapidly and depends upon gene expression in cotyledons of developing Vicia faba seeds. Protoplasma 231,
15-23.
99. Zhou Y, Qu H, Dibley KE, Offler CE, Patrick JW (2007). A suite of sucrose transporters expressed in coats of
developing legume seeds includes novel pH-independent facilitators. Plant Journal 49, 750-764.
100. Zhang W-H, Patrick JW and Tyerman SD (2007). Actin filaments modulate hypoosmotic-responsive K+
efflux channels in specialised cells of developing bean seed coats. Functional Plant Biology 34,
874-884.
101. Zhou Y, Setz N, Niemietz C, Qu H, Offler CE, Tyerman SD, Patrick JW (2007). Aquaporins and unloading of
phloem-imported water iin coats of developing bean seeds. Plant, Cell and Environment 30, 1566-1577.
102. Tegeder M, Tan Q, Crennan AK, Patrick JW (2007). Amino acid transporter expression and localisation
studies in pea (Pisum sativum). Functional Plant Biology 34, 1019-1028.
103. Koller CE, Patrick JW, Rose RJ, Offler CE, MacFarlane GR. (2008). Arsenic and heavy metal accumulation
by Pteris vittata L. and P. umbrosa R. Br. Bulletin of Environmental Contamination and Toxicology 80,
128-133.
104. Zhou Y, Chan K, Wang TL, Hedley CL, Offler CE, Patrick JW (2009). Intracellular sucrose communicates
metabolic demand to sucrose transporters in developing pea cotyledons. Journal of Experimental Botany
60, 71-85.
105. Dibley SJ, Zhou Y, Andriunas FA, Talbot MJ, Offler CE, Patrick JW, McCurdy DW. (2009). Early gene
expression programs accompanying trans-differentiation of epidermal cells of Vicia faba cotyledons into
transfer cells. New Phytologist 182, 863-877.
Reviews
106. Patrick, J. W. (1986). Hormonal control of assimilate partitioning. Plant Growth Regulator 14, 7 - 11.
107. Patrick, J. W. (1988). Assimilate partitioning in relation to crop productivity. HortScience 23, 33-40.
108. Patrick, J. W. (1989). Solute efflux at plant/micro-organism interfaces. Australian Journal of Plant
Physiology 16, 53 - 68.
109. Patrick, J. W. (1990). Sieve element unloading: cellular pathway, mechanism and control. Physiologia
Plantarium 78, 298 - 308.
110. Patrick, J. W. (1992). Assimilate partitioning in plants: Opportunities for improving agricultural
productivity. In 'Agricultural Frontiers. Opportunities and Risks' pp. 25 - 29. Proc. N.Z. Inst. Ag. Sci.
& Hort. Sci.
111. Patrick, J. W. (1993). Sink strength: Whole plant considerations. Plant, Cell & Environment 16, 1019 1020.
112. Patrick, J. W. and Offler, C. E. (1995). Post-sieve element transport of sucrose in developing seed.
Australian Journal of Plant Physiology 22, 681 - 702.
113. Patrick, J. W. and Offler, C. E. (1996). Post-sieve element transport of photoassimilates in sink regions.
Journal of Experimental Botany 47, 1165 - 1178.
114. Patrick, J. W. (1997). Phloem unloading: Sieve element unloading and post-sieve element transport.
Annual Review of Plant Physiology & Plant Molecular Biology 48, 191 - 222.
115. Lalonde, S. L., Bole, E., Hellmann, H., Ward, J. M., Patrick, J. W. and Frommer, W. B. (1999). A dual
function of plant sugar carriers in transport and sugar sensing. Plant Cell 11, 702-726.
116. Offler, C. E., Thorpe, M. and Patrick, J. W. (2000). Assimilate transport and partitioning: Integration of
structure, function and molecular biology. Australian Journal of Plant Physiology 27, 473-476.
117. Patrick, J. W. and Offler, C. E. (2001). Compartmentation of transport and transfer events in developing
seeds. Journal of Experimental Botany 52, 551-564.
118. Patrick, J. W., Zhang, W-H., Tyerman, S. D., Offler, C. E and Walker N. A. (2001). Role of membrane
transport in phloem translocation of assimilates and water. Australian Journal of Plant Physiology 28,
1–13.
119. Lalonde, S., Tegeder, M., Throne-Holst, M., Frommer, W. B. and Patrick, J. W. (2003).
and unloading of amino acids and sugars.
Plant Cell and Environment 26, 37-56.
120. Offler, C. E., McCurdy, D. W., Patrick, J. W. and Talbot, M. J. (2003).
for a special purpose.
Phloem loading
Transfer cells: Cells specialized
Annual Review of Plant Biology 54, 431-454.
121. Zhang W-H, Zhou Y, Dibley KE, Tyerman SD, Furbank RT, Patrick JW. (2007). Nutrient loading of
developing seeds. Functional Plant Biology 34, 314-331.
122. McCurdy DW, Patrick JW, Offler CE. (2008). Wall ingrowth formation in transfer cells; novel examples of
localized wall deposition in plant cells. Current Opinion in Plant Biology 11, 653-661.
123. Patrick JW, Stoddard FL. (2009). Physiology of flowering and grain filling in faba bean. Field Crops
Research (in press).
Book Chapters
124. Martin, P. M. and Patrick, J. W. (1965). Growth, development and yield in crop production. In
Fundamentals of Modern Agriculture. (C. Blake ed.). pp. 180 - 196. Sydney University Press.
125. Patrick, J. W. and Wareing, P. F. (1970). Experiments on the mechanism of hormone-directed transport. In
Plant Growth Substances. (Ed. D. J. Carr) pp. 695 - 700. (Springer-Verlag)
126. Wareing, P. F. and Patrick, J. (1975). Source-sink relations and the partition of assimilates in the plant. In
Photosynthesis and Productivity in Different Environments. (Ed. J. P. Cooper) pp. 481-499.
(Cambridge University Press: Cambridge, U.K.)
127. Patrick, J. W. (1976). Hormone-directed transport of metabolites. In Transport and Transfer Processes in
Plants. (Eds. I. F. Wardlaw and J. Passioura) pp. 433 - 446. (Academic Press Inc.)
128. Patrick, J. W. and Wareing, P. F. (1981). Hormonal control of assimilate movement and distribution. In
Aspects and Prospects of Plant Growth Regulators. (Ed. B. Jeffcoat) pp. 65 - 84. (DPRG and BPGRG
Symposium)
129. Patrick, J. W. (1982). Hormonal control of assimilate transport. In Plant Growth Substances. (Ed. P. F.
Wareing) pp. 669 - 678. (Springer-Verlag)
130. Patrick, J. W. (1984). Carbon partitioning. In Control of Crop Productivity. (Ed. C. J. Pearson) pp. 59
- 79. (Academic Press)
131. van Bel, A. J. E. and Patrick, J. W. (1984). Proton extrusion in seed coats of Phaseolus vulgaris L. In
Membrane Transport in Plants. (Eds. W. J. Cram, K. Janacek, R. Rybova and K. Sigler) pp. 434 - 435.
(John Wiley and Sons)
132. Offler, C. and Patrick, J. W. (1986). Cellular pathway and hormonal control of short-distance transfer in
sink regions. In Phloem Transport. (Eds. J. Cronshaw, W. J. Lucas and R. T. Giaquinta.) pp.
296-306. (Alan Liss: New York)
133. Patrick, J. W. (1987). Are hormones involved in assimilate transport? In Hormone Action in Plant
Development - A Critical Appraisal. (Eds. G. V. Hoad, M. B. Jackson and J. R. Lenton.) pp. 175 - 187.
(Butterworths)
134. Cram, W. J., Jacobs, E., Patrick, J. W. and Offler, C. E. (1990). Phloem unloading site of bean seed coats.
Kinetics of unloading of different cells by microautoradiography. In Membrane Transport in Plants and
Fungi. (Eds. M. J. Beilby, N. A. Walker and J. R. Smith) pp. 482 - 483. (University of Sydney Press)
135. Patrick, J. W. (1991). Control of phloem transport to and short-distance transfer in sink regions. In Recent
Advances in Phloem Transport and Assimilate Compartmentation. (Eds. J. L. Bonnemain, S. Delrot, J.
Dainty and W. J. Lucas) pp. 167 - 177. (Quest Editions, Paris)
136. Patrick, J. W., Offler, C. E., Wang, H. L., Wang, X., Jin, S., Zhang, W., Ugalde, T. D., Jenner, C., Wang, N.,
Fisher, D. B., Felker, F. C., Thomas, P. A. and Crawford, C. G. (1991). Assimilate transport in
developing cereal grain. In Recent Advances in Phloem Transport and Assimilate Compartmentation.
(Eds. J. L. Bonnemain, S. Delrot, J. Dainty and W. J. Lucas) pp. 233 - 243. (Quest Editions: Paris.)
137. Patrick, J. W. (1992). Sugar transport to the stalk. In Improvement of Yield in Sugar Cane Through
Increased Sucrose Accumulation. (Ed. J. R. Wilson). pp. 65 - 70. (CSIRO, Division of Tropical Crops
and Pastures)
138. Wang, H. L., Offler, C. E. and Patrick, J. W. (1995). Assimilate delivery pathways in the developing
wheat grain and their implications for the control of carbon transport. In: Carbon Partitioning and
Source-Sink Interactions in Plants. (Eds. W. J. Lucas & M. Madore). pp. 156 - 169. Proceedings 17th
Annual Symposium of Plant Physiology. (University of California, Riverside)
139. Patrick, J. W., Offler, C. E. And Wardlaw, I. F. (1998). Vascular integration and resource storage (Sections
5.3 to 5.6). In Plants in Action. (Eds. B. J. Atwell, P. E. Kriedemann & C. G. N. Turnbull). pp. 158 –
185. (MacMillan Education Australia)
140. Patrick, J. W., van Bel, A. J. E. and Offler, C. E. (2003). Seed development - nutrient loading. In
“Encyclopedia of Applied Plant Sciences”. (ed B. Thomas, D. Murphy and B. Murray). Pp. 1240-1249.
Academic Press. London.
141. Van Bel, A. J. E., Offler, C. E. and Patrick, J. W. (2003). Environmental regulation of growth and
development – sources and sinks. In “Encyclopedia of Applied Plant Sciences”. (ed. B. Thomas, D.
Murphy and B. Murray). Pp 724 – 734. Academic Press. London
142. Throne-Holst, M., Offler, C. E. and Patrick, J. W. (2006). Assimilate transport to developing seeds. In
“ The Encyclopedia of Seeds: Science, Technology and Uses”. (ed. J. D. Bewley, M. Black and P Halmer).
CAB International. Wallingford, UK.
143. Tegeder M, Rentsch D, Patrick JW (in press). Organic carbon transport. In “Plant Plasma Membrane” (ed. A
Murphy, B Schulz and W Peer). Plant Cell Monographs. SpringerVerlag. Heidelberg, Germany.
Non-refereed articles/reports
144. Conroy, B. and Patrick, J. W. (1973). An assessment of the biological impact of effluent from the
proposed abattoir at Farley.
145. Patrick, J. W. and Conroy, B. (1974). An assessment of the biological impact of the proposed dam at
Glennies Creek. Water Conservation and Irrigation Commission of NSW.
146. Patrick, J. W. And McDonald, K. (1974). Nutrient status of high-dune soil of the Central Coast of NSW.
Hunter Natural History 6, 219 - 227.
147. Patrick, J. W., Offler, C. E. and Orr, J. F. (1976). Environmental studies in the area of Kurri Kurri works
1975-76. ALCAN Report KK-4-76.
INVITED INTERNATIONAL SYMPOSIUM PRESENTATIONS
1. Patrick, J. W. (1975). Hormone-directed transport of assimilates. In Transport and Transfer Processes in
Plants. USA-Australian Agreement for Scientific and Technical Cooperation, Canberra, Australia.
2. Patrick, J. W. (1979). Hormone-directed transport of assimilates. In Hormonal Regulation of Plant Growth.
49th ANZAAS Congress, Aukland, New Zealand.
3. Patrick, J. W. (1981). Hormone-directed transport as a possible mechanism of assimilate distribution. In
Assimilate Partitioning. XIII International Botanical Congress, Sydney.
4. Patrick, J. W. (1982). Hormonal control of assimilate transport. In Hormonal Regulation of Assimilate
Partitioning. 11th International Conference on Plant Growth Substance, Aberystwyth, Wales, UK.
5. Patrick, J. W. (1984). Photosynthate unloading from seed coats of Phaseolus vulgaris L. Cellular pathway,
mechanism and control. 4th Minnesota Soybean Workshop. St. Paul, Minnesota, USA.
6. Patrick, J. W. (1985). Hormonal control of short-distance transfer. In Phloem Unloading. International
Conference on Phloem Transport. Asiloma, California, USA.
7. Patrick, J. W. (1986). Assimilate partitioning. In Yield: A Challenge for all Seasons. XXII International
Horticultural Congress. Davis, California, USA
8. Patrick, J. W. (1986). Are hormones involved in assimilate transport? 10th Long Ashton Symposium,
Bristol, UK.
9. Patrick, J. W. (1988). Solute efflux at plant/micro-organism interfaces. In Biotrophic Relationships.
Australian Society of Plant Physiology. Adelaide, SA.
10. Patrick, J. W. (1990). Control of phloem transport to and short-distance transfer in sink regions. In Sink
Metabolism. International Conference on Phloem Transport, Cognac, France.
11. Patrick, J. W. (1992). Control of transport to and short-distance transfer in sink regions. In Assimilate
Partitioning and Carbon Metabolism. American Society of Plant Physiologists, Pittsburgh, USA.
12. Patrick, J. W. (1992). Assimilate partitioning in plants. In Opportunities for Improving Agricultural
Productivity. New Zealand Society of Plant Physiologists. Christchurch, New Zealand.
13. Patrick, J. W. (1992). Sugar transport to the stalk. In Improvement of Yield in Sugar Cane Through
Increased Sucrose Accumulation. CSIRO, Division of Tropical Crops and Pastures.
14. Patrick, J. W. (1993). Control of phloem transport to and short-distance transfer in sink regions. In Enzymes
and Metabolism. XV Botanical Congress, Tokyo, Japan.
15. Patrick, J. W. and Offler, C. E. (1994). Post-phloem transport of sucrose in developing seed. In JS Hawker
Memorial Symposium. Australian Society of Plant Physiologists. Gold Coast, Qld, Australia.
16. Patrick, J. W., Offler, C. E., Franceschi, V. R. and Walker, N. A. (1995). Post-sieve element transport in
developing seeds of bean and wheat. In Phloem Loading and Unloading Mechanisms. International
Conference on Transport of Photoassimilates, Canterbury, UK.
17. Patrick, J. W. (1995) Sucrose transport. In First International Symposium on Sucrose Metabolism. Mar
del Plata, Argentia (declined).
18. Offler, C. E. and Patrick, J. W. (1996). Solute transport dynamics of plasmodesmata in stems and roots.
In Plasmodesmata Intercellular and Long Distance Communication. Third International Workshop on
Basic and Applied Research in Plasmodesmal Biology. Zichron-Yakov, Israel.
19. Patrick, J. W. and Offler, C. E. (1996) Transport and storage: current technologies and scope for future
research. In Biochemistry and Molecular Modification of Sucrose Accumulation in Sugarcane. CSIRO,
Division of Tropical Crops and Pastures, Brisbane.
20. Patrick, J. W. and Offler, C. E. (1998). Physiology of fruit filling. Identifying opportunities to increase
seed yield. In Towards Better Fruit Filling and Seed Quality in Grain Legumes. Centre for Legumes
in Mediterranean Agriculture, University of Western Australia, Perth.
21. Patrick, J. W. and Offler, C. E. (2000). Compartmentation of transport and transfer events in developing
seeds. In Cellular Compartmentation of Plant Metabolism. Society for Experimental Biology, Exeter.
UK.
22. Offler, C. E. and Patrick, J. W. (2000). Transfer cells – key players in apoplasmic/symplasmic transport.
In Cell-Cell Communication and Transport. Robertson Symposium on Plant Cell Biology, Canberra.
23. Patrick, J. W. (2000) Nutrient transfer from seed coat to embryo. In Nutrient Supply to the Developing
Seed. 8th International Symposium on Plant Seeds. Gatersleben, Germany (declined).
24. Patrick, J. W. (2000).
Membrane transport in movement of assimilates. In Membrane Transport in
Modern Plant Physiology: Mechanisms and Processes from Organelles to Whole Plants. Centre for Plant
Membrane Biology, Port Lincoln, SA.
25. Offler, C. E., Patrick,J. W., Talbot, M. J. and McCurdy, D. W. (2000). Regulation of transfer cell wall
deposition
--
A working model.
Western Australia.
In Plant Form and Function. Adaptations to Stress.
University of
Symposium Honouring Professor John Pate.
26. Patrick, J. W. and Offler, C. E.
(2000).
Do transfer cells transport nutrients and, if so, does their
structure confer specialised transport functions? In Plant Form and Function. Adaptations to Stress.
University of Western Australia.
27. Patrick JW (2003).
Symposium Honouring Professor John Pate.
Phloem unloading strategies: Free loaders and suckers. In International
Conference on Phloem Transport.
Bayreuth, Germany.
28. Patrick, J. W. (2004). Nutrient import into crop seeds.
In Seeds in the -omics Era. 9th International
Symposium on Plant Seeds. Gatersleben, Germany.
29. Patrick JW (2006). A quest to discover sucrose effluxers – developings seeds offer promise. In
International Symposium in Memory of Vincent R. Franceschi. Plant Cell: Structure-Function Relations.
Pullman USA.
30. Patrick JW (2007).
Regulation of nutrient import into sink tissues. In International Plant Vascular Biology
Conference. Taipei, Taiwan
CONJOINT PROFESSOR CHRISTINA E OFFLER BSc (Hons), PhD (Adel)
School of Environmental & Life Sciences
Faculty of Science & Information Technology
The University of Newcastle
Date Birth:
July 13th, 1943.
Research Interests: Nutrient transport and distribution in plants; phloem unloading; transfer cell
development and function.
Research Overview and Contributions:
Co-leader of a research team with Emeritus Professor John W Patrick focused on understanding transport
and distribution of nutrients in plants. Using developing seeds and fruits (grain legumes, wheat and tomato),
we have integrated structural and physiological studies to pinpoint the cellular location of regulatory
transport processes. This approach has allowed directed molecular analysis, resulting in cloning of key
regulatory genes (sucrose and amino acid transporters) and novel sucrose effluxes. A research focus on
transfer cells, cells specialised for membrane transport of nutrients, has provided the first unequivocal
evidence that these cells do facilitate membrane transport in developing seeds. Using a unique transfer cell
induction system developed by us we have advanced current understanding of transfer cell biology through
elucidating the pattern of deposition of their wall ingrowth labyrinths, identifying gene expression profiles
during transfer cell induction and exploring signalling of inductive process.
Key Reviews:
Offler CE, McCurdy DW, Patrick JW, Talbot MJ (2003) Transfer cells: Cells specialized for a special
purpose. Annual Review of Plant Biology 54: 431-453.
McCurdy DW, Patrick JW, Offler CE (2008) Wall ingrowth formation in transfer cells: novel examples of
localized wall deposition in plant cells. Current Opinion in Plant Biology DOI :
10.1016/j.pbi.2008.08.005
Other Contributions to Research:
Research Training: Research Higher Degree completions (9 PhD and 3 MSc); 18 Honours students.
Currently co- supervising 5 PhD students.
Invited speaker: International Symposium in Memory of Vincent R Franceschi, Washington State University,
Pullman WA, USA, June 2006 – transfer cells: special cells – special purpose.Conference Convenor: (with
Dr Michael Thorpe, New Zealand) International Conference on Assimilate Transport and Partitioning
(ICATP'99), Newcastle, Australia. Contributions published in two special issues of the Australian Journal of
Plant Physiology (AjPP 2000 vol. 27, Nos 6 and 8/9).Chair Editorial Advisory Committee: Australian
Journal of Plant Physiology (renamed Functional Plant Biology), Chair 2001 – 2003 - instrumental in
facilitating name change of journal. Committee Member 1998 – 2000
Administrative Contribution: Head of School, School of Environmental and Life Sciences, Faculty of
Science and Information Technology, 2003 – 2007.
Refereed Publications: 2003 - 2008
(53 peer-reviewed research publications and reviews prior to 2003)
Book Chapters
Patrick JW, van Bel AJE, Offler CE (2003) Seed Development - Nutrient Loading. pp. 724-734. In:
Encyclopedia of Applied Plant Science. (eds. B Thomas, D Murphy, B Murray). Academic Press.
London.
Van Bel AJE, Offler CE, Patrick JW (2003) Environmental Regulation of Growth and Development –
Sources and Sinks. pp. 1240-1249. In: Encyclopedia of Applied Plant Sciences. (eds. B Thomas, D
Murphy, B Murray). Academic Press. London.
Throne-Holst M, Offler CE, Patrick JW (2006) Assimilate transport to developing seeds. In: The
Encyclopedia of Seeds: Science, Technology and Uses. (eds. JD Bewley, M Black, P Halmer). CAB
International. Wallingford, UK.
Reviews
Offler CE, McCurdy DW, Patrick JW, Talbot MJ (2003) Transfer cells: Cells specialized for a special
purpose. Annual Review of Plant Biology 54: 431-453.
McCurdy DW, Patrick JW, Offler CE (2008) Wall ingrowth formation in transfer cells: novel examples of
localized wall deposition in plant cells. Current Opinion in Plant Biology Biology DOI :
10.1016/j.pbi.2008.08.005
Journal Articles
Aoki N, Scofield GN, Wang X-D, Patrick JW, Offler CE, Furbank RT (2004). Expression and localisation of
the wheat sucrose transporter TaSUT1 in vegetative tissues. Planta 219: 176-184.
Dibley SJ, Gear ML, Yang X, Rosche EG, Offler CE, McCurdy DW, Patrick JW (2005) Temporal and
spatial expression of hexose transporters in developing tomato (Lycopersicon esculentum) fruit.
Functional Plant Biology 32: 777-785.
Harrington GN, Dibley KE, Ritchie RJ, Offler CE, Patrick JW (2005) Hexose uptake by developing
cotylwdons of Vicia faba: physiological evidence for transporters of differing affinities and specificities.
Functional Plant Biology 32: 987-995.
Aoki N, Scofield GN, Wang X-D, Offler CE, Patrick JW, Furbank RT (2006) Pathway of sugar transport in
germinating wheat seeds. Plant Physiology 141: 1255-1263.Zhou Y, Qu H, Dibley KE, Offler CE,
Patrick JW (2007a). A suite of sucrose transporters expressed in coats of developing legume seeds
includes novel pH-independent sucrose facilitators. Plant Journal 49: 750-764.
Talbot MJ, Wasteneys GO, Offler CE, McCurdy DW (2007a) Cellulose synthesis is required for deposition
of reticulate wall ingrowths in transfer cells. Plant & Cell Physiology 48: 147-158.
Vaughn KC, Talbot MJ, Offler CE, McCurdy DW (2007) Wall ingrowths in epidermal transfer cells of Vicia
faba cotyledons are modified primary walls marked by localized accumulations of arabinogalactan
proteins. Plant & Cell Physiology 48: 159-168.
Koller CE, Patrick JW, Rose RJ, Offler CE, MacFarlane GR (2007) Pteris umbrosa R.Br. as an arsenic
hyperaccumulator: accumulation, partitioning and comparison with the established As hyperaccumulator
Pteris vittate. Chemosphere 66: 1256-1263.
Wardini T, Talbot MJ, Offler CE, Patrick JW (2007a) Role of sugars in regulating transfer cell development
in cotyledons of developing Vicia faba seeds. Protoplasma 230: 75-88.
Wardini T, Wang X-D, Offler CE, Patrick JW (2007b) Induction of wall ingrowths of transfer cells occurs
rapidly and depends upon gene expression in cotyledons of developing Vicia faba seeds. Protoplasma 231:
15-23.
Talbot JM, Wasteneys G, McCurdy DW, Offler CE (2007b). Deposition patterns of cellulose microfibrils in
flange wall ingrowths of transfer cells indicate clear parallels with those of secondary wall thickenings.
Functional Plant Biology 34: 307-313.
Zhou Y, Setz N, Niemietz C, Qu H, Offler CE, Tyerman SD, Patrick JW (2007b) Aquaporins and unloading
of phloem-imported water in coats of developing bean seeds. Plant, Cell & Environment 30: 1566-1577.
Offler CE, MacFarlane GR (2008) Arsenic and heavy metal
accumulation by Pteris vittata and P. umbrosa. Bulletin of Environmental Contamination and Toxicology.
80: 128-133.
Zhou Y, Chan K, Wang TL, Hedley CL, Offler CE, Patrick JW (2008) Intracellular sucrose communicates
metabolic demand to sucrose transporters in developing pea seed cotyledons. Journal of Experimental
Botany PMID: 18931350