SUPPLEMENTAL FIGURES Figure S1. Geographic distribution of Y-chromosome main lineages in the studied populations. Only haplogroups reaching frequencies of at least 3% in the combined dataset were plotted. Color code for Y-chromosome lineages as in the legend at the top right. Labels: ARB_CAL: Arbereshe of Calabria, ARB_SIC: Arbereshe of Sicily; SSI: Sicilians and SouthernItalians, ALB: Albanians, GRE: Greeks. Haplogroup composition for each community of Calabrian and Sicilian Arbereshe are detailed in the boxes at the top- and bottom-left of the plot respectively (Codes for single Arbereshe communities as in Table S1). Figure S2. Non-metric MDS plot based on STRs distances. Population codes as in Table S1. Symbols and color codes for ethnic-group affiliations as in the legend. Abbreviations: ARB_CAL: Arbereshe of Calabria, ARB_SIC: Arbereshe of Sicily; SSI: Sicilians and SouthernItalians, ALB: Albanians, GRE: Greeks. Figure S3. Phylogenetic haplotypes network of haplogroup E-V13. Each circle represents a different haplotype. Circles’ areas are proportional to haplotype frequencies. White circles represent median vectors. Population color codes as in the legend. Abbreviations: ARB_CAL: Arbereshe of Calabria, ARB_SIC: Arbereshe of Sicily; ITA: Sicilians and Southern-Italians, ALB: Albanians, GRE: Greeks. Red oval designates the Calabrian Arbereshe-specific cluster (EV13α) selected for age estimate. Figure S4. Phylogenetic haplotypes network of haplogroup I-P215(xM26,M223). Each circle represents a different haplotype. Circles’ areas are proportional to haplotype frequencies. White circles represent median vectors. Population color codes as in the legend. Abbreviations: ARB_CAL: Arbereshe of Calabria, ARB_SIC: Arbereshe of Sicily; ITA: Sicilians and SouthernItalians, ALB: Albanians, GRE: Greeks. Orange oval designates the Sicilian Arbereshe-specific cluster (I-P215α) selected for age estimate. Figure S5. Phylogenetic haplotypes network of haplogroup R-SRY10831.2. Each circle represents a different haplotype. Circles’ areas are proportional to haplotype frequencies. White circles represent median vectors. Population color codes as in the legend. Abbreviations: ARB_CAL: Arbereshe of Calabria, ARB_SIC: Arbereshe of Sicily; ITA: Sicilians and SouthernItalians, ALB: Albanians, GRE: Greeks. Red/orange oval designates the shared Calabrian/Sicilian Arbereshe-specific cluster selected for age estimate. Figure S6. Phylogenetic haplotypes network of haplogroup I-M223. Each circle represents a different haplotype. Circles’ areas are proportional to haplotype frequencies. White circles represent median vectors. Population color codes as in the legend. Abbreviations: ARB_CAL: Arbereshe of Calabria, ARB_SIC: Arbereshe of Sicily; ITA: Sicilians and Southern-Italians, ALB: Albanians, GRE: Greeks. Red oval designates the Calabrian Arbereshe-specific cluster (IM223α) selected for age estimate. Figure S7. Phylogenetic haplotypes network of haplogroup E-M123. Each circle represents a different haplotype. Circles’ areas are proportional to haplotype frequencies. White circles represent median vectors. Population color codes as in the legend. Abbreviations: ARB_CAL: Arbereshe of Calabria, ARB_SIC: Arbereshe of Sicily; ITA: Sicilians and Southern-Italians, ALB: Albanians, GRE: Greeks. Red and orange ovals designate the Calabrian (E-M123α) and Sicilian (E-M123β) Arbereshe-specific clusters respectively, which have been selected for age estimates. SUPPLEMENTAL TABLES Table S1. List of the Arbereshe and comparison populations included in the present study. For each population the geographic coordinates, the region and the language affiliation are detailed. N: sample size. Table S2. Sets of simulated parameters for both stationary and growth models. Table S3. Y-Chromosome STRs haplotypes and SNPs analysis results for the newly-typed samples of Arbereshe (N=150) and Albanian (N=223) populations. Table S4. Y-chromosome haplogroup composition in the newly-generated dataset. For each Ychromosome lineage the absolute number of individuals and the percentage frequency (between brackets) are reported. Frequencies of the 36 combined HGs used in comparative analyses are reported in bold, whereas the sub-lineages decomposition for all the SNPs genotyped in the present study are detailed in plain font. Table S5. Y-chromosome diversity parameters for the investigated populations based on both haplogroup frequencies (SNPs) and haplotype data (STRs). Table S6. Analyses of the molecular variance (AMOVA). Apportionment of molecular variance are in percentage (%) and based on both haplogroup frequencies (SNPs) and haplotype data (STRs).