STANDARD OPERATING PROCEDURE
SP # (number)
Page 1 of 2
Version: 1.0
Date: 5 May 2010
Revise by: 05-May-2013
Replaces: N/A
Written by: Colette Boskovic
Authorised by: EHS Officer
NMR Laboratory, School of Chemistry, The University of Melbourne
Conducting Experiments on the 400 MHz NMR Spectrometer
in the Masson Resonance Lab
1.
Introduction
This document covers the operation of the 400 MHz NMR spectrometer in the Masson
resonance laboratory.
2.
Scope
This document defines the operating procedures for all staff, students and visitors who use
the NMR spectrometer.
3.
Safety
All users must undergo a safety induction with Dr Hamish Grant.
4.
Licences and Permits
Upon completion of instrument training and safety induction, all users must supply an
"operation of hazardous equipment" permission form signed by Dr Hamish Grant and their
supervisor to the School of Chemistry safety officer.
5.
Training and Competency
All users must complete an instrument training session and safety induction with Dr Hamish
Grant.
Access to instrument requires an account and password.
6.
Risk Assessments
The risk assessment for operating the spectrometer is included as an attachment to this
document.
7.
Equipment and Maintenance / Handling and Storage / Labelling
Regular cryogen (liquid nitrogen and helium) fills are performed by trained staff using
appropriate PPE.
The presence of a strong magnetic field is labelled at the entry point.
An operating manual is available on the instrument computer.
8.
Operating Procedures
1.
Log in
2.
Eject standard/insert sample
3.
Select experiment
4.
Lock and shim
5.
Acquire
6.
Save file
7.
Eject sample/insert standard
8.
Lock and shim
9.
Exit.
STANDARD OPERATING PROCEDURE
SP # (number)
Page 2 of 2
Version: 1.0
Date: 5 May 2010
Revise by: 05-May-2013
Replaces: N/A
Written by: Colette Boskovic
Authorised by: EHS Officer
NMR Laboratory, School of Chemistry, The University of Melbourne
9.
Controls and Calibrations
Following the initial installation, further calibration is not required, other than the standard
chemical shift referencing, pulse calibration and probe tuning that are performed routinely by
experimenters.
10.
Waste Disposal
No waste materials are generated through operation of the spectrometer.
Samples are removed following completion of the experiment.
In the event of a broken sample, Dr Hamish Grant or another authorised person (Dr Colette
Boskovic, Dr Frances Separovic) must be informed immediately.
11.
Relevant Documents / References
School of Chemistry Safety Manual, Masson Resonance Laboratory Safety & Emergency
Procedures Manual, Risk Assessment for Operation and Routine Maintenance of the 400
MHz NMR Spectrometer.
All documents are accessible from the School of Chemistry safety website:
http://safety.chemistry.unimelb.edu.au/
12.
Signage / Summaries / Templates
Signage at entry point designates the presence of strong magnetic and radio-frequency fields.
Signage near the spectrometer indicates the 5 Gauss line.
13.
Appended Material
Risk Assessment for Operation and Routine Maintenance of the 400 MHz NMR
Spectrometer