Curriculum vitae
Noni Franklin-Tong, March 2014
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email
Date of birth:
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Vernonica Elsa Franklin-Tong
School of Biosciences, University of Birmingham, Edgbaston,
Birmingham, B15 2TT.
021 414 3702; Fax 021 414 5925
V.E.Franklin-Tong@bham.ac.uk
10-03-61
Age: 53
Female
British
EDUCATION
University of Birmingham: B.Sc. (Hons) Biological Sciences. Sept. 1979- July 1982.
Awarded B.Sc (Hons) 2i, July 1982, and the John Humphreys Memorial Prize in Botany.
Department of Genetics, University of Birmingham. (1982-1985). Ph.D.
The self-incompatibility system of Papaver rhoeas. I obtained my Ph.D. in March 1986.
EMPLOYMENT
Oct 2004-present
Oct 2001- Oct 2004
Dec 1997- Oct 2001
Dec 1992-Dec 1997
Sept 1992- Dec 1992
Sept 89-Aug 1992
July 1986-1989
Apr-July 1986
Oct-Dec 1985
Professor in Plant Cell Biology (personal Chair)
Reader in Plant Cell Biology
Lecturer, University of Birmingham
BBSRC Advanced Research Fellow, University of Birmingham (Independent Fellowship)
Research Fellow: University of Birmingham (Gatsby Charitable Foundation).
Research Fellow: University of Birmingham (AFRC Plant Molecular Biology Initiaitive)
Research Fellow: University of Birmingham (AFRC Cell Signalling Initiative)
Research Fellow: Unit for Applied Cell and Molecular Biology, Umeå University (NFR)
EMBO Short Term Fellowship, Umeå University (Sweden).
SUMMARY:
International recognition of my lab’s work on self-incompatibility and signalling in pollen tubes has been firmly
established in the last few years. I have an excellent track record obtaining research funding. I am currently PI
on 2 BBSRC grants. My research output includes 50 original research papers published in refereed
journals & 35 invited reviews. I have edited a book on self-incompatibility; this is the first monograph on this
topic for 30 years. I have 12 chapters in books and 33 invited reviews published. I have presented 52 invited
talks at International conferences; 79 invited university lectures abroad and in the UK; over 100 other
conference papers/posters. I am also pursuing potential applied aspect of my research, and a patent for
“Engineering Of Plants To Exhibit Self-Incompatibility”. I currently have a very high research profile and am held
in high esteem by my peers internationally, evidenced by the number of invitations to speak at prestigious
International meetings, to write articles for, e.g. Current Biology, Current Opinion in Plant Biology etc.. I referee
papers for many journals, including Nature, Science, Current Biology, as well as more specialist journals. I
review grants for BBSRC, NSF, USDA, HFSP. I was nominated for the Joseph Chamberlain Award for
Academic excellence in the University’s Founders Awards, 2010. I am a member of several Committees of
external bodies: Society for Experimental Biology (SEB) Plant Biology Committee: 1998-current; Council,
SEB 2002-2007; BBSRC BCB Committee Jan 2007-2009/10; BBSRC Data Sharing Policy Monitoring
Group; “Quality and Impact” review panel member: major evaluation of research at SLU, Uppsala Sweden,
2009; University of Birmingham R&KT Committee. 2007-2008. Elected Secretary General for the
International Association of Sexual Plant Reproduction Research (IASPRR): Aug 2010-current, Oxford
Brookes external member of Professorial promotions panel, 2010-current. I am also on the Editorial
Board of several International Journals: Associate Editor for Journal of Experimental Botany 2010-current;
Sexual Plant Reproduction, 2006-current; Frontiers in Plant Physiology (2011-current); Associate Editor for
Plant Signalling and Behavior (Landes) 2005-2010; Plant & Cell Physiology 2007-2010; Plant Journal Advisory
Board. 1997-2004. I am committed to a public engagement with science. I was a BBSRC Regional Coordinator
of the Schools Liaison Scheme (1999-2004). I have given presentations in a BBSRC “Communicating with the
Public” workshop (2001) and to school teachers at the annual ASE meeting (Liverpool, 2002). I have organised
& participated in several scientific demonstrations/activities for the public, including “Flower Power”(Victoria
Rooms, Bristol 2010), ThinkTank, Birmingham (2006), and was a judge at the Celebration of Young Scientists
and Engineers (2005).
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(i) RESEARCH ACTIVITIES
RESEARCH GRANTS FUNDED:
Funding value:
Jan 2010
BBSRC Research Grant: Investigating the PrpS-PrsS
(pollen & pistil S-determinant) interaction
(PI: VE Franklin-Tong, joint with Christoph Baumann,
D Sanders (York), J Rappoport, Y Gu (Bham); 36 months).
July 2008
£520,423
BBSRC Research Grant: Investigating the
phospho-regulation of pollen soluble inorganic
pyrophosphatases (PI: VE Franklin-Tong, joint with
Dr Scott White, Biosciences; 36 months).
July 2005
£558,763
BBSRC Research Grant: Characterisation of caspase-like
protein activated by the SI response in Papaver rhoeas.
June 2005
2003-2005
(36 months). PI: VE Franklin-Tong
£271,533
Royal Society International Fellowship –China.
Investigating the involvement of the p56 MAP kinase in
programmed cell death and actin reorganisation during
self-incompatibility in Papaver pollen tubes. (12 months).
PI: VE Franklin-Tong.
£18,200
BBSRC Research Grant: Investigating if SI-induced actin
disruption is involved in programmed cell death. (36 months).
PI: VE Franklin-Tong
£217,228
2004-2007
BBSRC Research Grant: SI in Papaver rhoeas: functional characterization of
the pollen linked gene, STM1. (PI: FCH Franklin, joint
with VE Franklin-Tong & Prof D. Sanders FRS (Univ. of York).
£242,266
2002-2006
BBSRC Research Grant: Control of p26 activity by a CDPK, its
function in pollen tube growth and the SI reaction in Papaver rhoeas.
(36 months). PI: VE Franklin-Tong, joint with F.C.H. Franklin,
School of Biosciences.
£176,660
Royal Society-NATO Fellowship for Galina Shevchenko.
Investigating actin binding proteins in pollen tubes, and their
possible roles in the SI response. (12 months). PI: VE Franklin-Tong
£12,720
BBSRC Research Grant: The involvement of programmed cell
death in the SI response in Papaver rhoeas. (18 months).
PI: VE Franklin-Tong
£103,252
BBSRC Research Grant: The role and mode of action of
SPH1/74: a novel class of protein signal molecule from
Arabidopsis thaliana. (36 months). PI: F.C.H. Franklin, joint with
VE Franklin-Tong & Dr JP Ride, School of Biosciences.
£226,836
2002-2003
2002-2004
2002-2005
2001-2003
BBSRC Research Grant: Investigation of the p52 MAPK signalling
cascade involved in the SI response in Papaver rhoeas (18 months).
PI: VE Franklin-Tong, joint with F.C.H. Franklin, School of Biosciences. £105,460
1998-2000
M ARIE CURIE BIOTECHNOLOGY GRANT: Characterization of the
alterations in the cytoskeleton and its role in the self-incompatibility
response in pollen tubes of Papaver rhoeas.(24 months). Joint PIs:
Dr VE Franklin-Tong & Dr AMC Emons,Wageningen University,
2
1998-2000
1998-2001
1997-2000
1995-1998
1992-1997
The Netherlands
ECU 110,184
BBSRC Research Grant: Characterization of key events in
the self-incompatibility response in Papaver rhoeas. (36 months).
PI: VE Franklin-Tong, joint with F.C.H. Franklin, School of
Biological Sciences
£205,547
BBSRC Research Grant: Analysis of Ca2+ signalling and
Ca2+ waves in the self-incompatibility (SI) response in
Papaver rhoeas. (36 months). PI: VE Franklin-Tong
£166,832
BBSRC Research Grant: Molecular and biochemical characterization of
protein phosphorylation involved in the self-incompatibility reaction in
Papaver rhoeas. (36 months). PI: VE Franklin-Tong, joint with
F.C.H. Franklin, School of Biological Sciences, University of Birmingham
£151,512
BBSRC Research Grant: Self-incompatibility in Papaver rhoeas:
functional analysis of the stigmatic S-protein. PI: F.C.H. Franklin,
joint with VE Franklin-Tong & J.P. Ride, School of Biological Sciences,
University of Birmingham.
£142,476
BBSRC Advanced Fellowship: (Previously AFRC
Personal Fellowship). Personal 5-year independent Fellowship.
£210,000
Other grants awarded
2006
2004
2003
2000
1999
1999
1999
1995
1993
1993
Royal Society Travel Grant To attend Plant and fungal Cytoskeleton
Gordon Research Conference. NE, USA, Aug 2006.
Royal Society Travel Grant To attend senescence & PCD Gordon Research
Conference. NE, USA, June 2004.
Visiting Professor: October 2003 to Universidad Nacional
Autonoma de Mexico, Facultad de Quimica, Departamento de Bioquimica.
Royal Society Travel Grant To attend Gordon Research Conference. NH, USA
HEFCE/JREI: “Fluorescence Imaging in the Life Sciences”.
(JK Heath, R Insall, MJ Wakelam, VE Franklin-Tong).
BBSRC special grant. Collaborative research project for
BN Snowman to visit Staiger lab. Purdue University, USA.
BBSRC ISIS grant. Collaborative visit to Staiger lab. Purdue University, USA
Cytonet Collaborative Research Initiative “Characterization
of the SI response in Papaver pollen using ratio-imaging”. U.S.-funded
travel grant to visit Prof. P.K. Hepler’s lab. UMass, Amherst, Mass. USA.
Japan Seed Association Award for Self-incompatibility Special Session,
Yokohama, Japan.
Royal Society Travel Grant To attend International Botanical Congress
and visits to labs in Australia.
POST-DOCTORAL FELLOWS EMPLOYED
1997-2000
1998-2000
1998-2000
1998-2000
1999-2000
1999-2005
2001-2004
2002-2003
2002-2006
2005-2006
2005-2006
2002-current
2005-current
2009-09
2008-2009
2010- 2011
2010- current
Dr J.J. Rudd
Dr N.D. Jordan
Dr KR Straatman
Dr A.Geitmann
Dr A Caryl
Dr MJ Wheeler
Dr K. Osman
Dr G. Shevchenko
Dr S.G. Thomas
Dr Shutian Li
Dr MJ Wheeler
Dr B. de Graaf
Dr M. Bosch
Dr Min Gong
Dr Huawen Zou (Chinese visiting research scholar for 12 months)
Dr Richard Tudor
Dr Javier-Andres Juarez-Diaz (Mexican visiting researcher)
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2010- current
2011- current
Dr Andrew Beacham
Dr Deborah Eaves
PHD STUDENTS SUPERVISED
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Jason J. Rudd (1992-1995). PhD awarded 1996.
Anne Rehmany (1993-1996) BBSRC Committee Studentship. PhD awarded 1997.
Sadie Whittaker. (1997-2000). School Studentship. PhD awarded 2001.
Benjamin N. Snowman (Oct. 1998- 2001) BBSRC Committee Studentship. PhD awarded 2002.
Natalie Poulter (Oct 2005- 2009) BBSRC Quota studentship.
Richard Tudor (June 2006-2009) BBSRC (joint with FCH Franklin)
Sabina Vatovec (Oct 2007-) BBSRC Quota studentship.
Katie Wilkins (Oct 2008-) BBSRC Quota studentship.
Ju-You Wu, from Department of Horticulture, Nanjing Agricultural University (China Scholarship Council
award to study in an international lab for 12 months).
Lijun Chai (Feb 2011) from Huazhong Agricultural University, Wuhan (1 year award to study in an
international lab for 12 months).
Zongchen Lin (Oct 2011 –current) – LES College studentship
Tammana Haque (Oct 2011 –current) – Commonwealth studentship
Carlos Ortiz-Flores (Oct 2011 –current) – Chilean scholarship
Internal PhD supervisor:
Emma Punt (PhD 2002), Narinder Tamber (PhD 2006), Bicheng Yang (PhD 2008), Joanne Whiley
PhD external examiner
Manchester, Exeter, Glasgow.
Undergraduate special project students
June- Aug 2007. James Bancroft: awarded a Wellcome summer studentship.
Oct 2006-June 2007. Sabina Vatovec: Slovenian project student.
SUMMARY OF RESEARCH (PUBLISHED)
I have been studying pollination and the self-incompatibility (SI) system of Papaver rhoeas (the Field Poppy) for 20
years. SI is a genetically controlled mechanism whereby self-fertilization is prevented. SI in Papaver rhoeas is
controlled by a single, multi-allelic S-locus, comprising pollen and pistil (stigma) S-detereminants. My lab has made
major contributions to elucidating the signalling pathways involved in pollen tube growth and self-incompatibility. I
have established an International reputation in this field of research, which is evident from the talks I have been
invited to give and the review articles I have been invited to write. My work over the last few years, in the form of
publications and especially presenting my work in the USA, has firmly established international recognition of the
work on self-incompatibility and signalling in pollen tubes as first class. My lab is clearly recognized as leading the
field in elucidating the mechanism of the self-incompatibility response. I have also made an impact in the broader
area of plant signal transduction and also in signalling to the actin cytoskeleton. I review some of the major findings
below.
During the early 1990s, using microinjection and calcium imaging, I firmly established a role for cytosolic free
Ca2+ (Ca2+i) acting as a second messenger in the regulation of growth of Papaver rhoeas pollen tubes during the SI
response. This was one of the pioneering studies on calcium imaging in living plant cells, in collaboration with
Prof AJ Trewavas. These studies firmly established that the pistil “S proteins” (recently renamed “PrsS”: “Papaver
rhoeas stigma S”) act as elicitors for the SI response and that increases in [Ca 2+]i were induced by the SI response,
suggesting that Ca2+ acted as a second messenger in SI. More recently, I established that influxes of Ca 2+ occur at
the shank of the pollen tube. I obtained evidence that points to a central role for inositol(1,4,5)trisphosphate-induced
calcium release and for the phosphoinositide pathway in the control of Ca 2+ fluxes and control of pollen tube growth.
This work also provided the first published evidence for calcium waves in plant cells, and was published in
The Plant Cell (1996). These Ca2+ imaging studies profoundly affected the model(s) of the signalling pathway(s)
regulating the SI response and led the field internationally in plant cell signalling at the time.
Other studies have investigated events downstream of the SI-induced Ca2+ transients, where we have made
significant breakthroughs. My lab recently established that programmed cell death (PCD) is responsible for
mediating SI in incompatible Papaver pollen. PCD is an important mechanism responsible for the controlled death
of targeted cells in animal cells, and recently good evidence has emerged for this in plant cells. The demonstration
that PCD is triggered in the SI response and that a PCD signalling pathway is responsible for the death of
incompatible pollen, made headline news when my lab’s work was published in the highly prestigious journal,
Nature, in May 2004. This work was picked up in Nature’s News and Views article. A press release was made on
the BBSRC website when the work was published and it was also mentioned as a highlight in BBSRC’s Annual
Report. This work attracted a lot of media attention, a phone interview from the leading national newspaper in
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60 min
Brazil, and commentary articles in Scientific American (in its science news column), Innovations Report,
Wissenschaft-online and Spectrum-direkt scientific news columns. This exciting development will be the focus of
future research.
My lab established that dramatic and rapid changes to the actin cytoskeleton are observed in incompatible
pollen tubes of P. rhoeas undergoing the SI reaction. These alterations are S-specific and strongly suggest that the
actin cytoskeleton is targeted by the signalling pathway(s) stimulated in the SI response; this was published in The
Plant Cell (2000).. We have shown that there is massive and sustained actin depolymerization triggered. This is the
first demonstration of measurement of actin in plant cells in response to S-specific biological stimulus, and was
published in The Plant Cell (2002). More recently, following up this story, my lab established link between actin
depolymerization and initiation and progression into programmed cell death (published in Journal of Cell Biology
2006). This article attracted a lot of media attention and was selected for a highlight JCB “In This Issue” section.
This article was also selected by the highly prestigious journal “Cell” in a “Leading Edge” (their equivalent to
Science's Editor's Choice and Nature's Research Highlights) article. We recently published further studies on actin
alterations, characterizing the SI-induced punctate actin foci and actin binding proteins that bind these structures in
Plant Physiology (2010).
My lab recently established that eukaryotic soluble inorganic pyrophosphatases could be phosphorylated
and that this inhibited their activity. Notably this is the first example of a eukaryotic soluble inorganic
pyrophosphatase being phosphorylated. This work was published in Nature (444, 490-493) in November 2006. This
article attracted a lot of media attention and was selected for “Editor’s Choice” highlight in Nature (Nov 2006). It was
the topic of a press release on the BBSRC website. I helped write this. The story was featured on a number of
websites around the world. This research was featured as a front cover feature on the front of the University of
Birmingham BUZZ magazine (May 2007) and also as an inside feature article. This research was also featured as a
full page, illustrated article in BBSRC’s “Business” Magazine (Spring 2007)
More recently, my lab published on studies of several caspase-like activities implicated in mediating
programmed cell death in SI, in the highly prestigious journal, Proceedings of the National Academy of
Sciences USA (November 2007). This paper presented characterization of the SI-induced caspase-like activities
in detail; our study provided novel insights into the temporal and spatial activation of plant caspase-like enzymes.
We showed that SI also activates a VEIDase and a LEVDase, and that the VEIDase plays a role in SI-induced
PCD. The DEVDase and VEIDase are activated remarkably rapidly: detectable within 1-2h after SI-induction; the
LEVDase activity peaks later. Importantly, we showed the first live-cell imaging of a DEVDase activity in a higher
plant cell; the SI-activated DEVDase has a cytosolic and nuclear localization. We also demonstrated that SI
induces a rapid and substantial cytosolic acidification, which matches the in vitro pH optima for the SI-induced
caspase activities. pH alterations during plant PCD have not previously been reported to our knowledge. Since both
cytosolic acidification and nuclear caspase localization are observed during apoptosis in animal cells, our data
provided striking parallels between SI-induced PCD and apoptosis in animal cells. Recent studies have
characterized an unusual VPE in Papaver pollen that is not involved in PCD (Plant Molecular Biology, 2010).
The latest major breakthrough in this system, was published in Nature in June 2009. This described the
identification of PrpS (“Papaver rhoeas pollen S”), the pollen S determinant, which is a small novel transmembrane
protein that interacts with the small secreted pistil determinant, which we previously called “S protein”, but have
now renamed “PrsS” (“Papaver rhoeas stigma S”). This paper described cloning of three alleles of a highly
polymorphic pollen-expressed gene, PrpS, from Papaver and provided evidence that this encodes the pollen S
locus determinant. PrpS is a single copy gene linked to the pistil S gene, PrsS. Sequence analysis indicates that
PrsS and PrpS are equally ancient and are likely to have co-evolved. PrpS encodes a novel ~20 kDa protein.
Consistent with predictions that it is a transmembrane protein, PrpS is associated with the plasma membrane. We
show that a predicted extracellular loop segment of PrpS interacts with PrsS and, using PrpS antisense
oligonucleotides, we demonstrate that PrpS is involved in S-specific inhibition of incompatible pollen. Identification
of PrpS represents a major advance in our understanding of the Papaver SI system. The identification of PrpS as a
novel cell-cell recognition determinant thus contributes to the available information regarding the evolution of selfnonself recognition systems, which also include histocompatibility systems in primitive chordates and vertebrates.
This milestone opens up the way for studies of the interaction (see below). A press release was made on the
BBSRC website and on the University web site.
Current ongoing research & future research focus
SI in P. rhoeas is an excellent system in which to investigate intracellular signalling. Significant progress has been
made and the studies have provided several promising avenues to follow for further understanding of SI and plant
responses to signals in general. It is anticipated that future studies will consolidate the work described. Signalling is a
ground-breaking area in plants at present, as is the topic of programmed cell death, which is an up-and-coming area
of research since its recent discovery in plants within the last decade. The response of the actin cytoskeleton to
signals is also a “hot” topic at present. The research is focused into 4 main areas:
1. Analysis of phosphorylation of soluble inorganic pyrophosphatases (sPPases) Pr-p26.1a/b. This is a
BBSRC-funded project. A major aim is to establish phosphorylation sites on for Pr-p26.1a/b using mass
spectrometry and site-directed mutagenesis to establish those involved in sPPase phospho-regulation.
Another is to clone and analyse the CDPK(s) involved in SI-induced phosphorylation of Pr-p26.1a/b.
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Functional analysis will analyse the impact of phospho-mediated changes in sPPase reaction kinetics and/or
protein stability.
2. Investigations into the nature of the pollen S determinant, PrpS as a receptor/ion channel, and its
interaction with the PrsS ligand. This will focus on aspect of the receptor-ligand interaction and very early
signaling events upstream of all the events so far characterised. Successful BBSRC grant proposal
(awarded Jan 2010) to carry this forward; grant started July 2010.
3. Pursuing possible commercial aspects of using SI, by investigating whether poppy SI can be
transferred to other species. This follows on from filing a patent in November 2008, based on very
preliminary data that suggested that transferring PrpS1 to Arabidopsis allowed it to recognise poppy
recombinant PrsS proteins resulting in the inhibition of PrpS1-GFP pollen germination. This is a very exciting
opportunity, and I obtained LES College KT Development funding (£20,000) to pursue obtaining further
data to consolidate the patent. I have also been in discussions with PBL, who are keen to market license this
application, which could be of immense value to plant breeders in the production of F1 hybrids. This has
resulted in marketing of our invention and also investment into research projects to provide further data to
support claims. Newly initiated collaboration with JIC, Norwich to investigate if the poppy SI system functions
in barley, as a model crop plant.
4. Continuing investigations into the integration of signals and targets involved in mediating SI in
incompatible pollen tubes. Emphasis will be given to the major components which appear to have
functional significance for modulating alterations in the cell. This will include examining the components
involved in modulating PCD and identifying the cellular targets for PCD. Since SI-stimulated PCD involves a
caspase-like activity and as caspase genes have not been identified in plants to date, an important goal will
be to try to purify the protein involved and clone the gene encoding it. Other studies planned will investigate
the MAPK, ROS and NO & other signalling components involved in SI in more detail, in particular, to
investigate whether the MAPK may interact with the actin and PCD signalling cascades. One current area is
the role of the vacuole in contributing to the SI response. It is thought that the disintegration of the vacuole
may be an important step in activation/progression of PCD, as we have data suggesting that it may be
involved in acidification of the cytosol, thereby allowing activation of the caspase-like activities. The longterm goal is to establish how the different components integrate and interact in what has turned out to be a
complex signalling network.
Commercialization of research outputs
I am attempting to commercialize the poppy SI determinants for improved plant breeding.
PATENT FILED: I filed a patent ((appl. number 0821812.5.) for “Engineering Of Plants To Exhibit SelfIncompatibility” on 28th November 2008. This was taken to International level (filed May 2011).
I have been actively engaging with PBL (Norwich) to commercialize the poppy SI patent. I have initiated a collaborative
project with Dr Wendy Harwood at the John Innes Centre (JIC), to move the poppy S-genes into barley to test their
functionality in a crop species. PBL have funded a project at JIC to help push this forward. This project is ongoing, with
new PhD students involved.
I have also recently initiated a further collaboration with Prof Zhenbiao Yang at SIPPE (CAS, Shanghai) to engineer
transgenic rice with the popy S-determinants to further test functionality and possibilities for plant breeding applications.
PUBLICATIONS
*Publications in which I am first author or senior author are indicated *
Original research papers in Journals –all refereed
1.
Barend H. J. de Graaf, Sabina Vatovec, Javier Andrés Juárez-Díaz, Lijun Chai, Kreepa Kooblall, Katie
A. Wilkins, Huawen Zou, F. Christopher H. Franklin and Vernonica E. Franklin-Tong. The Papaver
self-incompatibility pollen S-determinant, PrpS, functions in Arabidopsis thaliana. Current Biology
22 (2), 154-159. doi:10.1016/j.cub.2011.12.006
2.
Katie A. Wilkins, James Bancroft, Maurice Bosch, Jennifer Ings, Nicholas Smirnoff and Vernonica E.
Franklin-Tong (2011). ROS and NO mediate actin reorganization and programmed cell death in the
Self-Incompatibility
response
of
Papaver.
Plant
Physiology
156,
404-416.
DOI:
10.1104/pp.110.167510.
3. Natalie S. Poulter, Maurice Bosch,Vernonica Franklin-Tong (2011). Proteins implicated in mediating
self-incompatibility-induced alterations to the actin cytoskeleton of Papaver pollen. Annals of Botany.
doi:10.1093/aob/mcr022
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4. *Juyou Wu, Su Wang, Yuchun Gu, Shaoling Zhang, Stephen J Publicover and V. E. Franklin-Tong
(2011). Self-incompatibility in Papaver rhoeas activates non-specific cation conductance(s) permeable
to Ca2+ and K+. Plant Physiology 155, 963–973. DOI: 10.1104/pp.110.161927
5. *Maurice Bosch, Natalie S. Poulter, Ruth M. Perry, Katie Wilkins and V. E. Franklin-Tong (2010).
Characterization of a legumain/vacuolar processing enzyme and YVADase activity in Papaver pollen.
Plant Molecular Biology 74 (4), 381-393. DOI: 10.1007/s11103-010-9681-9.
6. *Natalie S. Poulter, Christopher J. Staiger, Joshua Z. Rappoport, and V. E. Franklin-Tong (2010).
Actin-Binding Proteins Implicated in the Formation of the Punctate Actin Foci Stimulated by the SelfIncompatibility Response in Papaver. Plant Physiol. 152: 1274-1283.
7. Liu J, Piette BMAG, Deeks MJ, Franklin Tong VE, Hussey PJ. 2010. A Compartmental Model Analysis
of Integrative and Self-Regulatory Ion Dynamics in Pollen Tube Growth, PLoS ONE, 5, 10, e13157.
8. *Wheeler, M.J., de Graaf, B.H.J., Hadjiosif, N.E., Perry, R.M., Poulter, N.S., Osman, K., Vatovec, S.,
Harper, A., Franklin, F.C.H & Franklin-Tong, V.E. (2009). Identification of the pollen self-incompatibility
determinant in Papaver rhoeas. Nature 459, 992-995.
9. *Poulter, N. S., Vatovec, S. and Franklin-Tong VE. (2008). Microtubules Are a Target for SelfIncompatibility Signaling in Papaver Pollen. Plant Physiol. 146, 1358-1367.
10. *Bosch M. & Franklin-Tong VE. (2007). Temporal and spatial activation of caspase-like enzymes
induced by self-incompatibility in Papaver pollen. Proc. Natl. Acad. Sci. USA. 104 (46) 18327-18332.
11. *S. Li, J. Samaj & V. E. Franklin-Tong. (2007). A MAP kinase signals to Programmed Cell Death
induced by Self-Incompatibility in Papaver pollen. Plant Physiol 145, 236–245.
12. *B.H.J. de Graaf, J.J. Rudd, M. J. Wheeler, R.M. Perry, E. M. Bell, K. Osman, F. C.H. Franklin & V. E.
Franklin-Tong (2006). Self-incompatibility in Papaver targets soluble inorganic pyrophosphatases in
pollen. Nature 444, 490-493. Selected for “Editor’s Choice” in Nature.
13. * Thomas, S. Huang , S. Li, C.J. Staiger and V.E. Franklin-Tong. (2006). Actin depolymerization is
sufficient to induce programmed cell death in self-incompatible pollen. S.G. Journal of Cell Biology 174,
221-229. **This article was selected for a highlight JCB “In This Issue” section and by Cell in a “Leading
Edge” (their answer to Science's Editor's Choice and Nature's Research Highlights; Aug 11th 2006
issue of Cell).
14. Geitmann, A, McConnaughey, W, Lang-Pauluzzi, I, Franklin-Tong, VE & Emons, AMC. (2004).
Cytomechanical properties of Papaver pollen tubes are altered after self-incompatibility challenge.
Biophysical.Journal 86, 3314-3323.
15. Geitmann, A, Franklin-Tong, VE & Emons, AMC. (2004). Ultrastructural analysis of the selfincompatibility response in Papaver rhoeas pollen reveal early and striking alterations to organelles. Cell
Death & Differentiation 11: 812-822.
16. Huang, S., Blanchoin, L., Chaudhry, F., Franklin-Tong, V.E. and Staiger, C.J. (2004) A gelsolin-like
protein from Papaver rhoeas pollen (PrABP80) stimulates calcium-regulated severing and
depolymerization of actin filaments. Journal of Biological Chemistry 279, 23364-23375.
17. *Thomas, S.G. & Franklin-Tong, VE. (2004) Programmed Cell Death is triggered by self-incompatibility in
Papaver pollen. Nature 429, 305-309.
18. *Rudd JJ, Osman, K., Franklin, F. C. H., Franklin-Tong V. E. (2003) Activation of a putative MAP
kinase in pollen is stimulated by the self-incompatibility (SI) response. FEBS Letters 547, 223-227
19. *VE Franklin-Tong, TL Holdaway-Clarke, KR Straatman, JG Kunkel, PK Hepler (2002). Involvement of
extracellular calcium influx in the self-incompatibility response of Papaver rhoeas. Plant Journal 29, 333345.
20. Snowman, B.N., Kovar, D.R., Shevchenko, G., Franklin-Tong, V.E., and Staiger, C.J. (2002). Signalmediated depolymerization of actin in pollen during the self-incompatibility response. Plant Cell 14:
2613-2626.
21. *K.R. Straatman, S.K. Dove, T. Holdaway Clarke, PK Hepler, JG Kunkel & VE Franklin-Tong (2001).
Calcium signalling in pollen of Papaver rhoeas undergoing the self-incompatibility (SI) response. Sexual
Plant Reproduction 14, 105-110. (Special Issue: “Frontiers in Sexual Plant Reproduction").
22. Jordan, ND, Ride, JP, Rudd, JJ, Davies, EM, Franklin-Tong VE, Franklin FCH (2000). Inhibition of selfincompatible pollen in Papaver rhoeas involves a complex series of cellular events . Annals of Botany 85
(supplement A), 197-202.
23. *Snowman, BN, Geitmann,A., Clarke, SR., Staiger, CJ, Franklin, FCH, Emons, AMC, Franklin-Tong,
VE (2000). Signalling and the cytoskeleton in pollen tubes of Papaver rhoeas. . Annals of Botany 85
(supplement A), 49-57.
24. * Geitmann, A, Snowman, BN, Emons, AMC, Franklin-Tong, VE (2000). Alterations to the Actin
Cytoskeleton of Pollen Tubes Are Induced by the Self-Incompatibility Reaction in Papaver rhoeas. Plant
Cell 12, 1239-1252.
25. * ND Jordan, FCH Franklin, VE Franklin-Tong (2000). Evidence for DNA fragmentation triggered in the
self-incompatibility response in pollen of Papaver rhoeas. Plant Journal 23 471-479.
26. Wheeler, M.J., Allan, A.C., Jordan, N.D., Rudd, N.D., Franklin-Tong, V.E., Franklin, F.C.H. (1999). The
intracellular events triggered by the self-incompatibility response in P. rhoeas. Protoplasma 208, 99-108.
7
27. Jordan, N.D., Kakeda, K., Conner, A., Ride, J.P., Franklin-Tong, V.E. and Franklin, F.C.H. (1999) Sprotein mutants indicate a functional role for SBP in the self-incompatibility reaction of P. rhoeas. Plant
J. 20, 119-126.
28. *Clarke, SR, Staiger, CJ, Gibbon, BC, and Franklin-Tong, VE (1998). A potential signaling role for
profilin in pollen of Papaver rhoeas. Plant Cell 10, 967-979.
29. Kurup, S., Ride, J.P., Jordan, N.D., Fletcher, G., Franklin-Tong V.E., Franklin, F.C.H. (1998).
Identification and cloning of related self-incomaptibility S genes in Papaver rhoeas and Papaver
nudicaule. Sex. Plant Reprod.11, 192-198.
30. Kakeda, K., Jordan, N.D., Conner, A., Ride, J.P., Franklin-Tong, V.E., Franklin, F.C.H. (1998)
Identification of residues in a hydrophilic loop of the Papaver rhoeas S protein that play a crucial role in
recognition of incompatible pollen. Plant Cell 10, 1723-1731.
31. *Rudd, J., Franklin, F.C.H. Lord, J.M. & Franklin-Tong, V.E. (1997) Ca2+ -independent phosphorylation
of a 68kDa protein is stimulated by the self-incompatibility response. Plant Journal 12, 507-514.
32. Calder, G.M., Franklin-Tong, V.E., Shaw, P.S., Drøbak, B.K. (1997) Calcium oscillations in plant cells:
initiation by rapid elevation in cytosolic free Ca2+ levels. Biochem Biophys. Res. Comm. 234, 690-694.
33. *Franklin-Tong, V.E., Hackett, G., and Hepler, P.K. (1997) Ratio-imaging of Ca2+i in the selfincompatibility response in pollen tubes of Papaver rhoeas. Plant Journal 12, 1375-1386.
34. Walker, E.A., Ride, J.P., Kurup, S., Franklin-Tong, V.E., Lawrence, M.J. & Franklin, F.C.H. (1996)
Molecular analysis of two functionally identical S 3 homologues of the self-incompatibility gene of
Papaver rhoeas from two different populations. Plant Molecular Biology 30, 983-994.
35. *Rudd, J., Franklin, F.C.H. Lord, J.M. & Franklin-Tong, V.E. (1996) Increased phosphorylation of a
26kDa protein is induced by the self-incompatibility response. Plant Cell 8, 713-724.
36. *Franklin-Tong, V.E., Drøbak, B.K., Allan, A.C. & Trewavas, A.J. (1996) Growth of pollen tubes of
Papaver rhoeas is regulated by a slow moving calcium wave propagated by inositol (1,4,5)
trisphosphate. Plant Cell 8, 1305-1321.
37. *Franklin-Tong, V.E., Ride, JP. & Franklin, F.C.H. (1995) Recombinant stigmatic self-incompatibility (S) protein elicits a Ca2+ transient in pollen of Papaver rhoeas. The Plant Journal 8, 299-307.
38. *Lawrence, M.J. & Franklin-Tong, V.E. (1994). The population genetics of the self-incompatibility
polymorphism in Papaver rhoeas. IX. Evidence of an extra effect of selection acting on the S-locus.
Heredity 72, 353-364.
39. Foote, H.G., Ride, J.P., Franklin-Tong, V.E., Walker, E.A., Lawrence, M.J. & Franklin, F.C.H. (1994).
cloning and expression of a novel self-incompatibility (S-)gene from Papaver rhoeas L. Proc. Natl. Acad.
Sci. (USA) 91, 2265-2269.
40. *Franklin-Tong, V.E., Ride, J.P., Read, N.D., Trewavas, A.J. & Franklin, F.C.H. (1993). The selfincompatibility reaction in Papaver rhoeas is mediated by cytosolic free calcium. Plant Journal 4, 163177.
41. Lawrence, M.J., Lane, M., O'Donnell, S. & Franklin-Tong, V.E. (1993). The population genetics of the
self-incompatibility polymorphism in Papaver rhoeas. V. The cross-classification of S-alleles of samples
from three natural populations. Heredity 71, 581-590.
42. Franklin, F.C.H., Franklin-Tong, V.E., Thorlby, G.J., Atwal, K.K., & Lawrence, M.J. (1992). Molecular
basis of the self-incompatibility mechanism in Papaver rhoeas L.. Plant Growth Regulation 11, 5-12.
43. Elleman, C.E., Franklin-Tong, V.E. & Dickinson, H.G. (1992). Pollination in species with dry stigmas the nature of the early stigmatic response & the pathway taken by pollen tubes. New Phytologist 121,
413-424.
44. *Franklin-Tong, V.E., Atwal, K.K., Howell, E.C., Lawrence, M.J. and Franklin, F.C.H. (1991). Selfincompatibility in Papaver rhoeas L.: there is no evidence for the involvement of stigmatic ribonuclease
activity. Plant, Cell and Environment 14, 423-429.
45. *Franklin-Tong, V.E., Lawrence, M.J. and Franklin, F.C.H. (1990). Self-incompatibility in Papaver
rhoeas L: inhibition of incompatible pollen is dependent on pollen gene expression. New Phytologist,
116, 319-324.
46. *Franklin-Tong, V.E., Lawrence, M.J., Ruuth, E., Franklin, F.C.H. (1989). Characterization of a
stigmatic component from Papaver rhoeas L. which exhibits the specific activity of a self-incompatibility
(S-) gene product. New Phytologist, 112, 307-305.
47. *Franklin-Tong, V.E., Lawrence, M.J. and Franklin, F.C.H. (1988). An in-vitro method for the
expression of self-incompatibility in Papaver rhoeas using stigmatic extracts. New Phytologist, 110, 109118.
48. Franklin-Tong VE, Lawrence MJ, Franklin FCH (1988) Self-Incompatibility in Papaver rhoeas. 1.
Characterization of the Stigmatic Component. Heredity 61, 286-286.
49. Franklin FCH, Jepson I, Franklin-Tong VE, Lawrence MJ (1988) Self-Incompatibility in Papaver rhoeas.
2. Cloning of Stigma Specific Genes. Heredity 61, 286-287.
50. Lawrence,M.J., Marshall,D.F., Fearon,C.H. and Curtis*,V.E. (* Curtis was my previous married name)
(1985). Gametophytic self-incompatibility re-examined: A Reply. Heredity, 54, 131-138.
8
Invited reviews for journals:
51. Eaves DJ, Flores-Ortiz C, Haque T, Lin Z, Teng N, Franklin-Tong VE.(2014) Self-Incompatibility in
Papaver: Advances in integrating the signalling network. Biochermical Transactions 42:370-6. doi:
10.1042/BST20130248
52. Smertenko, A. & Franklin-Tong, VE (2011). Organization & Regulation of the cytoskeleton in plant
programmed cell death. Cell Death & Differentiation 18, 1263-1270. doi:10.1038/cdd.2011.39
53. Noni Franklin-Tong (2011). Self-fertilization: article in: Brenner’s Encyclopedia of Genetics.
54. Wouter G. van Doorn, Eric P. Beers, Jeffery L. Dangl, Vernonica E. Franklin-Tong, Hiroo Fukuda,
Patrick Gallois6, Jean Greenberg, Ikuko Hara-Nishimura, Alan M. Jones, Maki Kawai-Yamada, Eric
Lam11, John Mundy12, Luis Mur, Morten Petersen, Andrei Smertenko, Michael Taliansky, Frank van
Breusegem16, Thomas Wolpert, Ernst Woltering, Boris Zhivotovsky, Peter V. Bozhkov (2011).
Morphological classification of plant cell deaths. Cell Death & Differentiation 18, 8, 1241-1246.
doi:10.1038/cdd.2011.36
55. VE Franklin-Tong (2010). Plant fertilization: Bursting pollen tubes! Current Biology 20, R681-683.
56. NS Poulter, MJ Wheeler, M. Bosch, VE Franklin-Tong.
(2010). Self-incompatibility in Papaver:
identification of the pollen S-determinant. Biochemical Transactions. 38, 588–592.
57. Staiger CJ, Poulter NS, Henty JL, Franklin Tong VE, Blanchoin L. 2010. Regulation of actin dynamics by
actin-binding proteins in pollen, Journal of Experimental Botany, 61 1969-1986.
58. MJ Wheeler, S. Vatovec & VE Franklin-Tong.(2010) The pollen S-determinant in Papaver: comparisons
with known plant receptor and protein ligand partners. Journal of Experimental Botany 61(7):2015-2025;
doi:10.1093/jxb/erp383
59. Bosch, M., Poulter, N.S., Vatovec, S., and Franklin-Tong, V.E. (2008). Initiation of Programmed Cell
Death in Self-Incompatibility: Role for Cytoskeleton Modifications and Several Caspase-Like Activities.
Molecular Plant 1, 879-887.
60. V. E. Franklin-Tong and C. W. Gourlay (2008). A role for actin in regulating apoptosis/programmed cell
death -evidence spanning yeast, plants and animals. Biochemical Journal 413, 389-404.
61. M. Bosch, N. Poulter, S. Vatovec, S. Li & V. E. Franklin-Tong. (2008). Initiation of Programmed Cell
Death in Self-Incompatibility: Role for Cytoskeleton Modifications and Several Caspase-Like Activities.
Molecular Plant 1(6):879-887; doi:10.1093/mp/ssn053 (Special issue on Plant Cell Biology).
62. S. Li & V. E. Franklin-Tong. (2008). Self-incompatibility in Papaver: A MAP kinase signals to
Programmed Cell Death. Plant Signaling & Behavior 3 (4) 243-245.
63. Bosch M. & Franklin-Tong VE. (2008). Self-incompatibility in Papaver: signalling to trigger PCD in
incompatible pollen. J. Exp. Bot. (Focus Issue, Feb 2008) 59, 481-490.
64. Wheeler, MJ & Franklin-Tong, VE. (2007). Specifying self-recognition: peptides lead the way.
Commentary. New Phytologist 175, 597-599.
65. Franklin-Tong VE (2007). Inhibiting self-pollen: self-incompatibility in Papaver involves integration of
several signaling events. Journal of Integrative Plant Biology 49, 1219-1226.
66. F. C.H. Franklin & V. E. Franklin-Tong. (2007) Self-incompatibility. Encyclopaedia of Life Sciences
(ELS), published by Wiley. Invited review. It is planned that this article will be included in a forthcoming
'spin-off' book from ELS, the Handbook of Plant Science (ed Keith Roberts).
67. *McClure B.A. & Franklin-Tong, V.E. (2006). Gametophytic self-incompatibility: understanding the
cellular mechanisms involved in “self” pollen tube inhibition. Invited Review: Planta 224 (2), 233 – 245.
68.B.K. Drøbak, V.E. Franklin-Tong and C. J. Staiger. (2004) The role of the actin cytoskeleton in plant
cell signalling. New Phytologist (Tansley Review). 163, 13-30.
69.
*V. E. Franklin-Tong and F. C.H. Franklin. (2003). Gametophytic self-incompatibility inhibits pollen
tube growth using different mechanisms. Trends in Plant Science 8 (12) 598-605.
70.*V. E. Franklin-Tong and F. C.H. Franklin. (2003). The different mechanisms with gametophytic selfincompatibility. Phil. Trans. Roy. Soc. Lond. B. 358, 1025-1032.
71.*Thomas S, Osman, K., de Graaf, B.H.J., Shevchenko, G., Wheeler, M.J., Franklin, F.C.H., & FranklinTong V. E. (2003) Investigating mechanisms involved in the self-incompatiblity response in Papaver
rhoeas. Phil. Trans. Roy. Soc. Lond. B. 358: 1033-1036.
72.*Rudd JJ, Franklin-Tong VE (2003) Signals and targets of the self-incompatibility response in pollen of
Papaver rhoeas. Journal of Experimental Botany 54: 141-148
73.
Staiger CJ, Franklin-Tong VE (2003) The actin cytoskeleton is a target of the self-incompatibility
response in Papaver rhoeas. Journal of Experimental Botany 54: 103-113.
74. Wheeler MJ, Armstrong SA, Franklin-Tong VE, Franklin FCH (2003) Genomic organization of the
Papaver rhoeas self-incompatibility S-1 locus. Journal of Experimental Botany 54: 131-139.
75. *Franklin-Tong, V.E. (2002). The difficult question of sex: the mating game. Current Opinion in Plant
Biology 5, 14-18.
76.*Franklin-Tong, V.E. (2002). Receptor-ligand interaction demonstrated in Brassica self-incompatibility.
Trends in Genetics, March 2002.
77.*Rudd, J.J., and Franklin-Tong, V.E. (2001). Unravelling response-specificity in Ca2+ signalling
pathways in plants cells. New Phytologist 151, 7-33. Tansley review.
9
78. Wheeler, M.J., Franklin-Tong, V.E. and Franklin, F.C.H. (2001). The molecular and genetic basis of
pollen-pistil interactions. New Phytologist 151, 565-584. Tansley review.
79.*Franklin-Tong, V.E. & F.C.H. Franklin (2000). INSIGHT article: Self-incompatibility in Brassica: The
elusive pollen S gene is identified! Plant Cell 12, 305-308.
80.*Rudd, J.J., and Franklin-Tong, V.E. (1999). Calcium signalling in plants. Cell and Molecular Life
Sciences. 55, 214-232. [Special multi-author review on Signaling in Plants].
81.*Franklin-Tong, V.E. (1999). Signalling and the modulation of pollen tube growth. Plant Cell 11, 727738. [Special Issue on Plant Cells and Organelles].
82.*Franklin-Tong, V.E. (1999). Signaling in pollination. Current Opinion in Plant Biology. 2, 490-495.
83.Wheeler MJ, Allan AC, Jordan ND, Rudd JJ, Franklin-Tong VE, Franklin FCH (1999) The intracellular
events triggered by the self-incompatibility response in Papaver rhoeas. Protoplasma 208: 99-106.
84.*Drøbak, B.K., Franklin, F.C.H., Shaw, P.S., Calder, G.M., Trewavas, A.J., Hepler, P.K. & FranklinTong, V.E. (1997). Second messenger-induced signalling events in pollen tubes of Papaver rhoeas.
Experimental Biology Online: 2:10 http://science.springer.de.ebo/tocs.t97.htm
85.*Franklin-Tong, V.E. Lawrence, M.J. & Franklin, F.C.H. (1995) Cell and molecular biology of selfincompatibility in flowering plants. International Review of Cytology. 158, 1-64.
86.*Franklin-Tong, V.E. and Franklin, F.C.H. (1993) Gametophytic Self-Incompatibility (SI) Systems:
contrasting mechanisms for Nicotiana and Papaver. Trends in Cell Biology 3, 340-345.
87.*Franklin-Tong, V.E. & Franklin, F.C.H. (1992). Gametophytic Self-incompatibility in Papaver rhoeas L.
Sexual Plant Reproduction 5, 1-7.
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Manuscripts submitted/in preparation
Lijun Chai, Richard L. Tudor, F. Christopher H. Franklin and Vernonica E. Franklin-Tong. PrMPK9-1, a
TDY-type MAPK, is functionally involved in mediating the Self-Incompatibility Response of Papaver.
The Self-Incompatibility Response of Papaver involves dramatic acidification of the incompatible pollen
tube cytosol. Katie A. Wilkins, Maurice Bosch, Tamanna Haque, Nianjun Teng and Vernonica E. FranklinTong.
Taking one for the team: Self recognition and cell suicide in pollen. Katie A. Wilkins and Noni (V. E.)
Franklin-Tong. JXB Special Issue.
Phosphoinositides play a role in the Self-Incompatibility Response of Papaver. Katie A. Wilkins, Teun
Munnik & Noni (V. E.) Franklin-Tong.
Michael J.Wheeler, Elizabeth M. Bell, Eric B. Holub, Jonathan Ride, Vernonica E. Franklin-Tong and F.
Christopher H. Franklin. SPH: a novel family of secreted proteins with members implicated in plant
defense regulation and development.
Chapters in books
1.
2.
3.
4.
5.
6.
7.
8.
Papaver rhoeas S-determinants and the signaling networks they trigger. Vernonica E. FranklinTong. Sexual Reproduction in Animals and Plants, Editors: Hitoshi Sawada, Naokazu Inoue, and
Megumi Iwano. Springer-Verlag.
Franklin-Tong VE, Self-Fertilization. In: Stanley Maloy and Kelly Hughes, editors. Brenner’s Encyclopedia of
Genetics 2nd edition, Vol 6. San Diego: Academic Press; 2013. p. 379–381.
S. Li and V.E. Franklin-Tong (2011). Modulating and Monitoring MAPK Activity During Programmed
Cell Death in Pollen. In: "Methods in Molecular Biology" (issue on Plant Kinases). Ed Arp Schnitger.
I have edited a book on Self-incompatibility, comprising 14 chapters; published by Springer in 2008.
V.E. Franklin-Tong (ed.) Self-Incompatibility in Flowering Plants – Evolution, Diversity, and
Mechanisms. Publ. Springer-Verlag Berlin Heidelberg 2008. This is the first monograph on this topic for 30
years.
V.E. Franklin-Tong (2008). Self-Incompatibility in Papaver Rhoeas: Progress in Understanding
Mechanisms Involved in Regulating Self-Incompatibility in Papaver. In: V.E. Franklin-Tong (ed.) SelfIncompatibility in Flowering Plants – Evolution, Diversity, and Mechanisms. Publ. Springer-Verlag Berlin
Heidelberg 2008
B.H.J. de Graaf, C. Lee, B. A. McClure, V.E. Franklin-Tong. (2006). Cellular mechanisms for pollen tube
growth inhibition in self-incompatibility. In: Plant Cell Monographs: “The Pollen Tube”, ed R. Malho.
Publisher: Springer-Verlag GmbH. ISSN: 1861-1370 (Paper) 1861-1362 (Online)
Thomas SG, Huang S., Staiger, CJ & Franklin-Tong, V.E. (2005) Signals and targets triggered by selfincompatibility: recognition of "self" can be deadly! In: Communication in Plants -Neuronal Aspects of Plant
Life. Edited by Baluska, F., Mancuso, S., Volkmann, D. published by Springer.
Geitmann, A. M. C. Emons & V.E. Franklin-Tong. (2000). Early cellular events in pollen tubes during the
self-incompatibility reaction. In: Geitmann, A., Cresti, M., and Heath, I.B. eds Cell Biology of Plant and
Fungal Tip Growth. NATO Science Series. Amsterdam: IOS Press.
10
9. *B.N.Snowman, Anja Geitmann, Anne Mie C. Emons and V.E. Franklin-Tong (2000). Actin rearrangements
in pollen tubes are stimulated by the self-incompatibility (SI) response in Papaver rhoeas L. In: Actin: a
dynamic framework for multiple plant cell functions. Eds Baluska, Barlow, Staiger, Volkmann.
10. *Franklin-Tong, V.E., Lawrence, M.J., Ride, J.P. & Franklin,. F.C.H. (1994) The molecular and cellular
biology of gametophytic self-incompatibility in Papaver rhoeas.(Invited review). In: Genetic control of selfincompatibility and reproductive development in flowering plants. Ed. EG Williams, RB Knox, AE Clarke.
Kluwer Academic, Dordrecht, The Netherlands. pp 42-66.
11.
*F.C.H. Franklin, K.K. Atwal, J.P. Ride & V.E. Franklin-Tong. (1994) Progress towards elucidating the
molecular basis of the Papaver rhoeas self-incompatibility system. In: Molecular and Cellular Aspects of
Plant Reproduction, SEB Seminar Series 55. Scott and Stead (eds). Cambridge University Press. 173-191.
12.
*Franklin-Tong, V.E., Atwal, K.K., Thorlby, G.J. & Franklin,F.C.H. (1993) Molecular Biology of Selfincompatibility in Papaver rhoeas. In: Current Topics in Molecular Genetics (Invited review). Ed. J. Menon.
Research Trends, in collab. with the Council of Scientific Research Information, Trivandrum, India. pp 9-14.
13. *Franklin-Tong, V.E., Thorlby, G.J., Lawrence, M.J. & Franklin,. F.C.H. (1992). Recognition signals and
pollen responses in the incompatibility reaction in Papaver rhoeas. In: Angiosperm Pollen and Ovules: Basic
and Applied Aspects (ed. Mulcahy, DL Bergamini-Mulcahy, G & Ottaviano, E).Springer-Verlag, New York.
pp 84-93.
14. *Franklin, F.C.H., Hackett, R.M. & Franklin-Tong, V.E. (1992). The molecular biology of self-incompatible
responses. In: Callow & Green (eds.) Perspectives in Plant Cell Recognition. SEB Seminar Series. (April,
1991). Cambridge University Press.pp 79-103.
Editorial work
1. EDITORIAL WORK ON J. EXP. BOT. SPECIAL ISSUE (2003).
Editor of the Special Issue of J. Exp. Bot. vol 54, no. 380 (2003) entitled: “Plant Reproductive Biology” (18
papers) that resulted from the SEB symposium.
2. EDITORIAL WORK ON J. EXP. BOT. SPECIAL ISSUE (2010).
Co-editor of the Special Issue of J. Exp. Bot. vol 61, no. 7 (2010) entitled: Pollen Tube Dynamics, Tip
growth and the Control of Pollination (10 papers). This resulted from two symposia at the PMB
Congress in St Louis, Oct 2009.
3.
EDITORIAL WORK ON J. EXP. BOT. SPECIAL ISSUE (2011).
Editor of the Special Issue of J. Exp. Bot. that resulted from the International Congress of Sexual Plant
Reproduction in Bristol, Aug 2010.
Published conference abstracts
1. Recognition of "self": conversations that commit “self” pollen to suicide: Reproductive Biology Keynote
Melbourne, IBC J Melbourne, IBC july 2011uly 2011
2. Caspases and signaling to PCD in self-incompatible pollen: Maurice Bosch, Katie A. Wilkins, Richard L
Tudor & Noni (V. E.) Franklin-Tong. Melbourne, IBC July 2011
3. Self-incompatibility in Papaver: signalling to trigger PCD in incompatible pollen. Franklin-Tong, VE.
Comparative Biochemistry & Physiology: SEB Annual meeting, Glasgow. March 2007.
4. Characterisation
of
caspase-like
activities
induced
in
Papaver
pollen
by
self-incompatibility. Comparative Biochemistry & Physiology: SEB Annual meeting, Glasgow. March 2007.
5. Shutian Li, Steven G. Thomas, Shanjin Huang, Christopher J. Staiger and Vernonica E. Franklin-Tong
(2006. Signals and cellular targets involved in PCD induced by self-incompatibility. FESBP Congress, Lyon,
France. July 16-21, 2006.
6. Steven G. Thomas, Shanjin Huang1, Shutian Li, Christopher J. Staiger1 and Vernonica E. Franklin-Tong
(2006). Signals and cellular targets involved in PCD induced by self-incompatibility. 20th International
Congress of Biochemistry and Molecular Biology (20th IUBMB 2006), Kyoto, Japan. June 18-23, 2006.
7. Identification and Functional Analysis of the Pollen Determinant of Self-Incompatibility in Papaver rhoeas.
BJH de Graaf, NE Hadiosif, NS Poulter, RM Perry, K Osman VE Franklin-Tong & FCH Franklin. Keystone
Meeting, Coeur d’Alene, Idaho, USA. March 2007.
8. Role of actin-binding proteins in remodelling of actin cytoskeleton during the SI response in Papaver
rhoeas pollen tubes. Shevchenko, G., Franklin-Tong, VE. 14th FESPB Congress, Krakow, Poland. Aug
2004.
9. A soluble inorganic pyrophosphatase is inactivated during the self-incompatibility response in Papaver
rhoeas pollen. BHJ de Graaf, JJ Rudd, EM Bell, FCH Franklin, VE Franklin-Tong. (2004). In: Recent
Advances in Inorganic Pyrophosphatase Research, ed SA White. Pp 110-114. July 2004.
10. An actin filament severing protein may function to inhibit tip growth during the self-incompatibility response
of poppy pollen. Huang S, Chaudhry F, Franklin-Tong, VE, Staiger, CJ. ASPB, Hawaii 2003.
11. Pollen profilin: a complex regulator of actin dynamics. Staiger, CJ, Kovar, DR, Chaudhry, F, Matsumoto, T.,
Snowman, B, & Franklin-Tong, VE. ASPB Annual Meeting, Denver, CO, USA. Aug 2002.
11
12. *Franklin-Tong, V.E. The pollen SI response involves actin depolymerization. SEB annual meeting,
Swansea, April 2002. Comparative Biochemistry and Physiology Part A 132 (2002) 125–137.
13. NATO Advanced Research Workshop: Cell Biology of Plant and Fungal Tip Growth. Siena, Italy. June 1923, 2000. Geitmann, A., Franklin-Tong, V. E., Emons, A.M.C. Till death do us part: Papaver pollen tubes
undergo programmed cell death upon contact with a self-incompatible stigma.
14. *Franklin-Tong, V.E. Mechanisms of pollen tube inhibition in Papaver rhoeas. Frontiers in Sexual Plant
Reproduction. Albany, NY State, USA. October 2000.
15. *Whittaker, S., Rudd, J.J., Clarke, S.R., Franklin-Tong, V.E. (1999). Manganese stimulation of
serine/threonine protein kinases in pollen of Papaver rhoeas. SEB Edinburgh, March 1999. J. Exp Bot.
16. Geitmann, A. Franklin-Tong V.E., Emons, A.M.C. (1999). The role of the cytoskeleton in signal
transduction in pollen tubes. 6th International Botanical Microscopy Meeting. St Andews University, 25-29
March 1999. Proceedings of the Royal Microscopical Society.
17. Pollen profilin: a regulator of actin polymerization and protein phosphorylation. Staiger, CJ, Kovar,DRA,
Gibbon, BC, Franklin-Tong, V.E., Clarke, SA, Zonia, LE. ASPP annual meeting, Madison, Wisconsin,
USA. June 1998.
18. *Franklin-Tong, V.E., Shaw, P.J., Calder, G.M., Trewavas, A.J. &Drøbak, B.K. (1997). Use of confocal
laser scanning microscopy in the study of second messenger-induced signalling events in pollen tubes of
Papaver rhoeas. Animal & Cell Biology Topics: [ACP2.6] 64. Society for Experimental Biology Annual
Meeting.
19. Franklin, FCH., Kakeda, K., Hearn., MJ., Franklin-Tong ,VE., & Ride JP.(1996). Pollen pistil recognition
during the self-incompatibility reaction in Papaver rhoeas. J. Exp. Bot. 47, 1.
20. Rudd, J.J., Franklin-Tong ,VE. & Franklin, FCH. (1996). Biochemical characterization of pollen protein
phosphorylation associated with the self-incompatibility reaction in Papaver rhoeas J. Exp. Bot. 47, 10.
21. Jordan, ND., Kakeda, K., Ride, JP., Franklin-Tong ,VE., & Franklin, FCH.(1996). Functional analysis of the
stigmatic S protein from Papaver rhoeas. J. Exp. Bot. 47, 1.
22. *Rehmany, AP., Franklin, FCH., Ride JP. & Franklin-Tong ,VE.(1996). Molecular characterization of Gproteins in pollen of Papaver rhoeas. J. Exp. Bot. 47, 13.
23. Franklin, FCH., Kakeda, K., Franklin-Tong ,VE., & Ride JP.(1996). Functional analysis of mutant S
proteins of Papaver rhoeas. Plant Reproduction ’96: 14th Congress of Sexual Plant Reproduction. Lorne,
Melbourne, Australia. 16.
24. Franklin, FCH., Kakeda, K., Franklin-Tong ,VE., & Ride JP.(1996). Construction and analysis of S protein
mutants in Papaver rhoeas. Banbury Conference, Cold Spring Harbor, New York, USA, 1996.
25. *Franklin-Tong, VE., Ride, JP. & Franklin, FCH. (1995) Molecular investigations of the control and
elicitation of self-incompatibility in Papaver rhoeas. J. Cell. Biochem. 19A, 136.
26. Kurup, S., Kakeda, K., Ride, JP., Franklin-Tong, VE. & Franklin, FCH. (1995) Mutational analysis of Sgenes from Papaver rhoeas. J. Exp. Bot. 46, 38.
27. Rudd, J.J., Franklin-Tong ,VE., Lord, JM., & Franklin, FCH. (1995). Specific hyperphosphorylation of a
pollen protein occurs during the self-incompatibility reaction in Papaver rhoeas. Signalling in Plant
Development, Cold Spring Harbor, New York, USA. p98.
28. *Franklin, FCH. and Franklin-Tong, VE. (1994) 13th Annual Symposium: Self-incompatibility in Papaver
rhoeas: a novel gametophytic S-gene. Current topics in plant biochemistry, physiology and molecular
biology: the biology of communication in plants. University of Missouri, Columbia, USA.
29. *Franklin, FCH., Ride JP. & Franklin-Tong ,VE. (1994) Molecular and cellular studies of self-incompatibility
in Papaver rhoeas. Plant Physiol. 5, 11.
30. *Franklin, FCH., Ride, JP. & Franklin-Tong, VE. (1993) Progress towards elucidating the molecular basis
of the Papaver rhoeas self-incompatibility system. J. Exp. Bot. 44, 8.
31. *Franklin-Tong, V.E., Thorlby, G.J., Atwal, K.K. Lawrence, M.J. and Franklin, F.C.H.(1991). Selfincompatibility in Papaver rhoeas: Molecular basis of the pollen-stigma interaction. Fundacion Juan March
Serie Universitaria 258, 60-61.
32. *Franklin-Tong, VE., Thorlby, GJ., Lawrence, MJ. & Franklin, FCH. (1990) Self-incompatibility in Papaver
rhoeas: Effect of an incompatible response on gene expression in pollen. Physiologia Plantarum 79, 244.
33. *Franklin-Tong, V.E. Lawrence, M.J. Ruuth, E. Jepson, I. and Franklin, FCH (1988). Molecular Biology of
self-incompatibility in Papaver rhoeas. Genome 30, suppl. 1, 291.
34. Franklin, FCH., Franklin-Tong, VE. & Lawrence, MJ. (1988) Self-incompatibility in Papaver rhoeas:
Cloning of stigma specific genes. Heredity 61, 287.
INVITED SPEAKER -INTERNATIONAL MEETINGS
1.
2.
3.
4.
1st International Symposium on Pollination and Fertilization. Tai’an, China, October 19-21, 2014
Symposium on Plant Sexual Reproduction, Academia Sinica, Taipei, Taiwan, Oct 15-17 2014.
Programmed Cell death Symposium, Barcelona, Oct 2013
German Botanical Congress, Tubingen Oct 2013
12
5. SEB symposium: reactive oxygen species, stress & PCD. Florence, June 2013
6. International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants. Nagoya, Nov
2012.
7. 22nd IASPRR, Melbourne. Invited speaker. Feb 2012.
8. Mt Tai 1st International Plant Reproductive Symposium
9. Plant Reproductive Development Symposium: Shanghai Jiao Tong University, China, August 2011
10. XVII International Botanical Congress- Melbourne, Australia, 23-30 July 2011- KEYNOTE SpeakerReproductive Biology Symposium
11. XVII International Botanical Congress- Melbourne, Australia, 23-30 July 2011- Symposium on Programmed
cell death in plant development and defense
12. XXI International Congress for Plant Sexual Reproduction, Bristol August 2010.
13. XVII Congress -Federation of European Societies of Plant Biology (FESPB) Valencia, July 4-9, 2010
14. International Symposium of Cell-Cell Communication in Plant Reproduction, Nara. March 2010.
15. 9th International Plant Molecular Biology (IPMB) Congress, October 2009 in St. Louis, Missouri.
16. 6th ISEM 2009. Symposium “Morphological features of programmed cell death across the eukaryotic
kingdom” Sept. 2009. Centennial Hall, Kobe University, Japan.
17. Phoenix 2009- Protein Complexes in signalling and development. Glasgow, June 2009.
18. Biochem. Soc. Meeting. Rothampsted, Sept 2009 (invited, but had to cancel).
19. Self-incompatibility in Papaver: signaling to programmed cell death in incompatible pollen and
identification of the pollen S-determinant. 3rd NAIST Global COE International Symposium, NAIST,
Nara, Japan. Nov 2008.
20. Self-Incompatibility in Papaver: Identification of the Pollen Self-Incompatibility Determinant.
Frontiers in Sexual Plant Reproduction III, Tucson, Arizona. October 17th-19th, 2008.
21. Self-incompatibility in Papaver: Identification of the pollen self-incompatibility determinant and
recent progress in characterizing signalling events triggered in incompatible pollen
22. 20th International Congress of Genetics (ICG), Berlin. July 2008.
23. Signalling to Programmed Cell Death in self-incompatible pollen. SEB session on Cell Biology of Plant
Development. Marseilles. July 2008.
24. Self-incompatibility in Papaver: signalling cascades trigger PCD in incompatible pollen. Society for
Experimental Biology Annual Meeting. Glasgow, March 2007
25. Actin depolymerization is sufficient to induce programmed cell death in self-incompatible pollen.
Gordon Research Conferences- GRC “Plant and Fungal Cytoskeleton”, Andover, NH, USA, Aug. 20-25
2006.
26. Signals and targets of self-incompatibility: recognition of "self" can be deadly! (Invited Session
Chair) FESBP Congress, Lyon, France. July 16-21, 2006.
27. Signals and cellular targets involved in PCD induced by self-incompatibility. (Symposium speaker)
20th International Congress of Biochemistry and Molecular Biology (20th IUBMB 2006), Kyoto, Japan. June
18-23, 2006.
28. How to stop a pollen tube? Signalling to the targets of self-incompatibility in Papaver pollen.
(Keynote speaker). 2nd International Symposium on Plant Neurobiology. May 22-26 2006, Beijing, China.
29. Signals and targets triggered by self-incompatibility: recognition of "self" can be deadly!
Symposium on Plant Neurobiology. Florence, Italy, May 2005.
30. Plenary lecture at the Irish Plant Scientists Meeting, University College Dublin, April 2005 (declined due to
other commitments).
31. Death of a pollen tube: Papaver uses programmed cell death to inhibit incompatible pollen. Frontiers
in Sexual Plant Reproduction II. Albany, NY State, USA. Oct 15-17, 2004.
32. Death of a pollen tube: Papaver uses programmed cell death to inhibit incompatible pollen.
Challenges in Plant Sciences: Symposium of the Zurich-Basel Plant Science Center, October 8, 2004.
33. The self-incompatibility system operating in Papaver rhoeas: recent advances. EMBO Practical
Course on Plant Reproduction, Institute of Genetics & Developmental Biology, Chinese Academy of
Sciences, Beijing, China. September 25 Aug- 7 Sept. 2004.
34. Self-Incompatibility involves actin depolymerization … is gelsolin involved, and does this signal to
PCD? Gordon Research Conference, Plant and fungal cytoskeleton, Andover, NH, USA. August 2004.
35. Self-Incompatibility triggers PCD in poppy pollen tubes: is signalling to the actin cytoskeleton
involved? Gordon Research Conference: Plant Senescence and Cell Death. Mt Holyoke College, MA,
USA. June 2004.
36. The different mechanisms of Gametophytic self-incompatibility. Royal Society Discussion Meeting:
Mechanisms regulating gene flow in flowering plants. London, Dec. 2003.
37. Signalling and targets in the self-incompatibility response of Papaver rhoeas. Ascona, Swizerland,
June 2003. Plant Gametophytes: evolution, development and function.
38. The targets of the SI signalling cascade indicate mechanisms involved in SI in Papaver rhoeas.
International Symposium on Plant Self-Incompatibility, Nara, Japan, Sept. 2003.
39. The targets of the SI signalling cascade indicate mechanisms involved in SI in Papaver rhoeas.
Japanese Society of Breeding, Annual Meeting, Kobe, Japan. Sept. 19, 2003.
13
40. The actin cytoskeleton is a target for self-incompatibility. SEB Annual meeting, Swansea, April 2002.
41. The actin cytoskeleton is a target for self-incompatibility. Gordon Conference on Plant actin &
cytoskeleton. New Hampshire, USA. August 2002.
42. Inhibition of pollen tube growth in the self-incompatibility response of Papaver rhoeas. Gordon
Conference on Developmental Physiology. New Hampshire, USA. July 2000.
43. Mechanisms of pollen tube inhibition in Papaver rhoeas. Frontiers in Sexual Plant Reproduction.
Albany, NY State, USA. October 2000.
44. Recognition, signalling and responses in the SI response of Papaver rhoeas. Nagoya, Japan, Jan.
1999.
45. Signalling and the cytoskeleton in pollen tubes of Papaver rhoeas. Pollen-Stigma Interactions
International meeting St Catherines College, University of Oxford, July 1999
46. Nara Institute of Science & Technology (NAIST), Nara, Japan. Workshop on Cell Signalling in Plants.
March 7, 1997. Signal Transduction pathways in plants, in particular calcium signalling.
47. Second messenger-induced signalling events in pollen tubes of Papaver rhoeas. SEB International
Symposium: April 1997.
48. Signalling in the self-incompatibility response in Papaver rhoeas. Third Cytonet Retreat,
Breckenridge, Colorado, USA. May 1997.
49. Molecular investigations of the control and elicitation of self-incompatibility in Papaver rhoeas.
Keystone Symposia on Molecular and Cellular Biology: Plant Cell Biology: Mechanisms, molecular
machinery, signals and pathways. Taos, New Mexico, USA, Jan. 1995.
50. The self-incompatibility response in P. rhoeas.Second Cytonet Retreat, Kona, Hawaii, May 13-16 1995.
51. Signalling in pollen of Papaver rhoeas- involvement of Ca2+ in the modulation of pollen tube growth.
Cold Spring Harbor Laboratory Meeting: Signalling and Plant Development, New York, USA, Oct 1 1995.
52. Molecular investigations of the control and elicitation of self-incompatibility in Papaver rhoeas L.
22nd Aharon Katzir-Katchalsky Conference on Plant Molecular Biology, Köln, Germany, Oct 2-6 1994.
53. Self-incompatibility in Papaver rhoeas: a novel S-gene whose pollen response is mediated by
[Ca2+]i. XVth International Botanical Congress, Tokyo (Japan), Aug. 1993.
54. Mechanisms involved in the self-incompatibility system of Papaver rhoeas. Self-incompatibility
Special Session: Yokohama Kokusai, Japan, August 1993.
55. Self-incompatibility in Papaver rhoeas. Rank Prize Fund Mini Symposium on Genetical Variation in the
study of Plant Processes, Grasmere, May 1992.
56. The self-incompatibility response in Papaver rhoeas is mediated by cytosolic calcium. 24th Lunteren
Lectures on Signal Transduction in Microorganisms and Plants, Lunteren (The Netherlands), Sept. 1992.
57. Recognition, signals and the pollen response in the incompatibility reaction in P. rhoeas.
International Symposium on Angiosperm Pollen and Ovules, Como (Italy), June 1991.
58. Molecular biology of self-incompatibility in P. rhoeas. Genetical Society: 16th International Congress of
Genetics, Toronto (Canada), August 1988.
INTERNATIONAL DEPARTMENTAL LECTURES
1. Departamento de Biología Molecular de Plantas; UNAM Instituto de Biotecnología, Cuernavaca, Mexico.
Feb 2014.
2. Depto. de Bioquimica, Facultad de Quimica, UNAM. Mexico City, Feb. 2014.
3. University of Osnabrück, Germany, June 2013.
4. Dept Biology, Wuhan University. Sept 2011
5. Dept Life Sciences, Peking University. Sept 2011
6. SIPPE, Chinese Academy of Sciences, Shanghai: Recognition of “self” in Papaver: what’s involved in
making “self” pollen stop? August 2011
7. Shanghai Jiao Tong University, China, August 2011
8. Institute of Botany, Beijing China, March 2011
9. Institute of Genetics &Developmental Biology, Chinese Academy of Sciences, Beijing, March 2011
10. Huangzhong Agricultural University, Wuhan China, March 2011
11. Nanjing Agricultural University China, March 2011
12. Depto. de Bioquimica, Facultad de Quimica, UNAM. April/May 2010. Recognition of "self" can be
deadly: self-incompatibility signalling networks trigger programmed cell death in pollen
13. Depto. de Bioquimica, Facultad de Quimica, UNAM. April/May 2010. Recognition of "self" can be
deadly: receptor-ligand interactions and signalling networks that trigger programmed cell death in
pollen
14. Departamento de Biología Molecular de Plantas; Instituto de Biotecnología, Cuernavaca, Mexico. April/May
2010. Recognition of "self" can be deadly: receptor-ligand interactions and signalling networks that
trigger programmed cell death in pollen
14
15. Department of Biological Sciences, Graduate School of Science, The University of Tokyo.March 2010.
Self-incompatibility signalling networks: conversations to commit “self” pollen to suicide
16. Department of Applied Biological Science, Tokyo University of Science. March 2010. Pollen tube
signalling networks: conversations that tell pollen which way to go and whether to live or die
17. Department of Applied Biological Science, Tokyo University of Science. March 2010. Recognition of
"self" can be deadly: receptor-ligand interactions and signalling networks that trigger programmed
cell death in pollen
18. University of Missouri, Columbia, USA (Nov 2009).
19. Gulbenkian Institute, Portugal. June 2008.
20. Botanisches Kolloquium – University of Bonn. Dec 2007.
21. University of Turku, Finland. March 2007.
22. Biology Department, University of Massachussetts, Amherst, USA. June 2004. NRE special summer
seminar. Death of a pollen tube: Papaver uses programmed cell death to inhibit incompatible pollen.
23. Plant Cytoskeleton Group, Purdue University, West Lafayette, USA. August 2004.
24. Self-Incompatibility involves both PCD and actin depolymerization. Are the two linked?? Death of a
pollen tube: Papaver uses programmed cell death to inhibit incompatible pollen. Biology Department,
Northwestern University, Xian, P.R. China. Sept 2004.
25. Death of a pollen tube: Papaver uses programmed cell death to inhibit incompatible pollen. Institute
of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of
Sciences, Shanghai, P.R. China. September 2004.
26. Death of a pollen tube: Papaver uses programmed cell death to inhibit incompatible pollen. State
Key Laboratory of Plant Physiology & Biochemistry, China Agricultural University, Beijing, P.R. China.
September 2004.
27. Death of a pollen tube: Papaver uses programmed cell death to inhibit incompatible pollen. CNRSINRA-ENSL-UCB Lyon, France. October 2004.
28. Death of a pollen tube: Signals and Responses triggered by Self-Incompatibility in Papaver reveal
several novel mechanisms to stop a pollen tube. Universidad Nacional Autonoma de Mexico (UNAM),
Facultad de Quimica, Departamento de Bioquimica, Mexico City, Oct. 2003.
29. Death of a pollen tube: Signals and Responses triggered by Self-Incompatibility in Papaver reveal
several novel mechanisms to stop a pollen tube. Institute for Biotechnology, UNAM, Cuernavaca,
Mexico, Oct. 2003.
30. Signalling cascades in self-pollen inhibition. March 2001. Plant Biology department, University of
Wageningen, The Netherlands.
31. Self-incompatibility protein-mediated signaling to growth inhibition in poppy pollen tubes.
Department of Botany & Plant Sciences, University of California, Riverside, California U.S.A. Aug 2000.
32. Inhibition of pollen tubes by self-incompatibility: many ways to stop a pollen tube.
33. Biology Department, University of Massachussetts, Amherst, Mass. USA. Oct 2000.
34. The actin cytoskeleton and signalling during the SI response of Papaver rhoeas. Dept. of Biological
Sciences, Purdue University, West Lafayette, USA. May 1999.
35. Cell-cell recognition and signalling in the self-incompatibility response of Papaver rhoeas. National
Institute of Agrobiological Resources (NIAR), Tsukuba, Japan. March 1997.
36. Cell-cell signalling in the self-incompatibility response of Papaver rhoeas. RIKEN (Frontier Research
Programme), Wako, Japan. March 1997.
37. Calcium signalling in the self-incompatibility response of Papaver rhoeas. British Embassy, Tokyo,
Japan. Plants as useful tools in Biotechnology. March 1997.
38. Cell-cell recognition and signalling in the self-incompatibility response of Papaver rhoeas. Hitachi
Advanced Research Laboratory, Japan. March 5, 1997.
39. Signal Transduction pathways in plants, in particular calcium signalling. Japan Tobacco (JT) Plant
Breeding and Genetics Research Laboratory, Iwata, Japan. March 1997.
40. Signalling in the self-incompatibility response in Papaver rhoeas. University of Wageningen, The
Netherlands, October 31st, 1997. To the Graduate school of Experimental Plant Sciences.
41. Self-incompatibility in Papaver rhoeas. Department of Biological Sciences, Purdue University, West
Lafayatte, Indiana, USA. May 1995.
42. Self-incompatibility in Papaver rhoeas: how is S-specific inhibition of pollen tube growth elicited?
Biology Department, University of Massachussetts, Amherst, Mass. USA., Sept 1995.
43. A novel S-gene from Papaver rhoeas elicits a Ca2+-mediated signal transduction pathway in pollen.
Plant Molecular Biology Laboratory, Rockefeller University, New York, USA. April 1994.
44. Calcium signalling in the self-incompatibility response in Papaver rhoeas. Institut fur Angewandte
Genetik, University of Hannover (Germany), April 1993.
45. SI in Papaver rhoeas: a new gametophytic S-gene. Plant Cell Biology Research Centre, University of
Melbourne (Australia). August 1993.
46. Self-incompatibility in Papaver rhoeas: a new gametophytic S-gene. Department of Genetics,
University of Adelaide (Australia). August 1993.
15
47. Self-incompatibility in Papaver rhoeas: a novel, calcium-mediated gametophytic S-gene. MaxPlanck-Institut fur Zuchtungsforschung, Cologne (Germany). December 1993.
48. Self-incompatibility in Papaver rhoeas: molecular basis for the pollen-stigma interaction. Fundacion
Juan March International Meeting on Biology: Flower Development, Madrid (Spain), March 1991.
49. Investigating the molecular basis of self-incompatibility in Papaver rhoeas. Department of Genetics,
Michigan State University, Lansing (U.S.A.), August 1988.
50. Investigating the molecular basis of self-incompatibility in Papaver rhoeas. Department of Biology,
State University of New York, Albany, N.Y. (USA.), Sept 1988.
51. The self-incompatibility system in Papaver rhoeas, the field poppy. Unit for Applied Cell and Molecular
Biology, Umea University, Umea (Sweden), Nov. 1985.
UK MEETINGS & UK DEPARTMENTAL LECTURES
1. ICMB, University of Edinburgh, Jan 2014
2. John Innes Centre, Norwich. July 2013.
3. School of Biological Sciences, University of York. May 2009. Self-incompatibility: cell-cell recognition
and communication using a novel pollen S-determinant, PrpS.
4. School of Biological and Biomedical Sciences, Durham University (Molecular Cell Biology seminar series).
Feb 2009.
5. School of Biological Sciences, University of Bristol. Jan 2009. Self-incompatibility in Papaver: Signaling
to PCD and identification of the pollen S-determinant:PrpS.
6. Department of Plant Sciences, University of Oxford. Feb 2008.
7. Plant Sciences, University of Glasgow, Feb. 2008.
8. School of Biosciences, Plant Sciences Division, University of Nottingham. Jan 2008.
9. Society for Experimental Biology (SEB) Annual Meeting (Canterbury, March 2006). Investigating the
involvement of the p56 MAPK in programmed cell death and actin reorganization during selfincompatibility in Papaver pollen. Invited talk by Dr Shutian Li - selected from submitted abstract in
“Developments in Plant Biology” session.
10. School of Biosciences, University of Birmingham. February 2006.
11. Department of Biological Sciences, University of Exeter. November 2005. Sex and contraception
in plants: signals and targets result in suicide of incompatible pollen.
12. Department of Biological Sciences, University of Durham. November 2005. Sex and contraception
in plants: signals and targets result in suicide of incompatible pollen.
13. School of Biosciences, University of Birmingham, May 2005. Inaugural lecture. Sex and contraception
in plants: a conversation ending in suicide.
14. School of Biological Sciences, University of Manchester, December 2004. Death of a pollen tube: Papaver
uses programmed cell death to inhibit incompatible pollen tubes.
15. Department of Plant Sciences, University of Oxford. January 2002. Self-incompatibility in Papaver
rhoeas: signalling cascades involved in self-pollen inhibition.
16. Department of Plant Sciences, University of Cambridge. October 2002 Self-incompatibility in Papaver
rhoeas: signalling cascades involved in self-pollen inhibition.
17. Calcium 2001, National workshop, HRI Wellesbourne, July 2001. Calcium signalling in the SI response
of Papaver rhoeas.
18. Department of Botany, University of Nottingham. October 2000. Inhibition of pollen tubes by selfincompatibility: many ways to stop a pollen tube.
19. School of Biological Sciences, School seminar, Feb. 1998. The SI response of Papaver rhoeas.
20. Calcium 98, National workshop, HRI Wellesbourne, July 2 nd 1998. Calcium signalling in the SI response
of Papaver rhoeas.
21. Biotechnology Forum: Future light-based Quantitation in Biotechnology, University of Birmingham, June 23
1997. Confocal & ratiometric imaging of [Ca2+]i in the self-incompatibility response in Papaver pollen.
22. University of Birmingham, School of Biological Sciences, Mini-Symposium on plant genetics and plant
breeding. September 1997. Self-incompatibility in Papaver rhoeas.
23. Royal Holloway University of London, Plant group seminar November 1997. Molecular analysis of the SI
response in Papaver.
24. Department of Botany, University of Leicester, January 1995. Self-incompatibility in Papaver rhoeas: a
model cell-cell recognition and signalling system.
25. School of Biological Sciences, University of Birmingham, March 1994. Molecular analysis of the SI
response in Papaver.
26. Plant Cell and Molecular Biology Group, School of Biological Sciences, University of Birmingham, January
1993. Microinjection of living pollen tubes: a new way in which to investigate self-incompatibility in
Papaver rhoeas.
27. Centre for Plant Sciences, I.C.M.B., University of Edinburgh, December 1991. Recognition, signals and
pollen responses in the self-incompatibility reaction of Papaver rhoeas.
16
28. Plant Biochemistry and Molecular Biology Group, School of Biology, University of Warwick, December
1991. Recognition, signals and pollen responses in the self-incompatibility reaction in Papaver
rhoeas.
29. Plant Cell and Molecular Biology Group, School of Biological Sciences, University of Birmingham, March
1990. Looking at the self-incompatibility response in pollen of Papaver rhoeas.
30. Department of Botany, University of Nottingham. November 1990. SI in Papaver rhoeas: The molecular
basis of the pollen-stigma interaction.
31. School of Biological Sciences, University of Birmingham, October 1988. Molecular biology of selfincompatibility in Papaver rhoeas, the Field poppy.
32. Unilever research group, Sharnbrook, Oct. 1987. The self-incompatibility system in Papaver rhoeas.
33. Department of Genetics, University of Birmingham, July 1985. Biology of the self-incompatibility
polymorphism in Papaver rhoeas, the Field poppy.
17
(ii) Teaching activities
Teaching Admin
1. Degree Label Leader for Plant Biology. Aug 2008- 2010. Responsibility for coordinating and
overviewing content and delivery of all Plant Biol. teaching over all 3 years).
2. Module Leader, 2nd year Plant Biology. Aug 2008 – 2010. Responsibility for delivering and organising
2nd year Plant Biology)
3. SECOND YEAR TUTOR for the School of Biosciences. 2002-2004.
4. STUDENT EXCHANGE TUTOR for the Biological Sciences degree programme.1998 –2003.
Teaching activities
1. 2nd year Field trip to Finse, Norway. July 2010, 2011.
A 1 week intensive field trip with ~20 undergraduate students, amounting to ~80 contact hours,
involving project work, lectures and field work.
2. Lectures in module BIO 237 (2nd year undergraduate Plant Biology module). 2000- 2013.
I have developed and teach 12 integrated set of lectures on the theme of Signals and responses in
plants. This incorporates lectures on plant responses to light, hormones, mechanical stimuli, gravity,
and developmental stimuli, as well as development and reproduction.
3. Practical class in module BIO 237 (2nd year undergraduate Plant Biology module). 20052012. I have produced a new practical class on pollen tube growth and its modlation for 2nd year Plant
Biol BIO237, with help and support from Bob Hall. This involves observations, measurements, pooling
of data across the class & drawing conclusions from results. This has been developed with Dr J
Pritchard to also involve “dry” practicals associated with the write-up, involving training in analyzing and
presenting data in the form of a scientific paper.
4. Lectures in module BIO 395 (Final year undergraduate Plant Molecular and Cell
Biology) 1997- 2011. I currently provide 12 lectures. Feedback from peer observation indicate my
lectures & handouts are good.
5. Lectures in Module BIO 124, BNat Sci students (>360 students) 1999- 2011.
I currently provide 4 lectures on basic animal cell biology: secretory pathway (x2), cytoskeleton (x2).
6. Practical class for module BIO 237 (2nd year undergraduate Plant Biology module).
2005- 2011. I developed a new practical on pollen tube growth and components involved in
modulating growth. This involves experimental organization, data collection and analysis. The practical
is written up for assessment in the form of a scientific paper. 4 sessions of 3 h =12 h.
7. Supervision of final year undergraduate project students (4-6 per year). 1996- 2012.
The projects are independent research orientated laboratory projects. They are generally based on
investigating potential functional components involved in either signal transduction or mechanisms
involved in pollen tube growth/ self-incompatibility in pollen. The feedback in recent years has been
excellent -students have enjoyed the experience and appreciated the care taken in supervising them.
8. Supervision of Final year Dissertation students (4-8 per year). 1997-2011.
Dissertation topics offered by myself are on a variety of topics in the area of plant cell signalling;
apoptosis and programmed cell death; alterations in the cytoskeleton. These are literature review
research projects, which are closely supervised. I meet fortnightly undergraduate dissertation students
on individual basis, advising and directing reading, and suggesting organisation of material for the final
dissertation write-up. This also involves reading and correcting drafts.
9. Personal Tutor to Undergraduates. 1997 to 2012
18
10. I have previously have acted as Personal Tutor for up to 35 undergraduate students a year (when
dealing with Exchange students). Recently this has been a more realistic number, at ~18
undergraduate tutees p.a.
11. Lectures and Practicals on Basic Cytogenetics to the MSc Applied Genetics, MSc
CUPGR students. 1999-2004. (~25 students). Comprised 5 h lectures, 10 h pracs. I developed the
practicals with Dr SJ Armstrong.
12. Lectures on Self-incompatibility to the MSc Applied Genetics students. 1999- 2005.
(~25 students). 12 h lectures. A challenging course, as the background of the intake is highly variable.
External Teaching activities




Lectures at EMBO Summer School, IGDB, Beijing. 2004.
Lectures at Summer School at Shanghai Jiao Tong University, China, August 2011. For Chinese
PDRA students.
Developed & provided a set of slides for the Gatsby online resource for Plant Biology HE teaching in my
area of research (invited by Celia Knight).
Lectures at Summer School, University of Osnabrück, Germany, Sept 2012. For German PDRA
students.
19
(iii) ACADEMIC CITIZENSHIP- External activities & services to the community
Active membership of national or international research evaluation panels & other relevant
committees, societies and funding bodies
1. Elected Secretary General for the International Association of Sexual Plant Reproduction
Research (IASPRR): Aug 2010-2014. The purpose of the IASPRR is to stimulate scientific research
and promote contact between scientists undertaking research in sexual plant reproduction and related
subjects through conferences, congresses, workshops, databases, and by the exchange of information
concerning the entire range of activities in the field of plant sexual reproduction research. The IASPRR is
affiliated with the International Union of Biological Sciences (IUBS). The Association holds an International
Symposium every two years and the Board appoints an organizing committee and acts as advisory
members of the organizing committee.
2. Member of BBSRC Biochemistry & Cell Biology Committee Jan 2007-Feb 2009
Invited to apply by BBSRC.
3. Member of BBSRC Committee D. May 2009-2010
Retained on the committee as a core member after reorganization of Committee structure.
4. Member of evaluation panel for “Quality and Impact” at SLU, Upsala, Sweden. May 2009.
A major external review of research carried out at SLU (Swedish University of Agricultural Sciences)
Uppsala, Sweden. May 2009. This was based on my internationally recognized expertise and impact in
research in plant sciences. I was a member of a team assessing two plant science research units, headed
by Chris Leaver, FRS..
5. Member of BBSRC Data Sharing Policy Monitoring Group 2008-current
Invited to apply by BBSRC.
6. SOCIETY FOR EXPERIMENTAL BIOLOGY (SEB) PLANT BIOLOGY COMMITTEE
Elected Nov 1998-2011. Committee work centred around the Society’s educational & publishing
activities and the Annual Meeting, which is the largest conference for plant scientists in Europe. In addition,
I organized a 2 day session on Plant Reproductive Biology for SEB annual Meeting March 2002, jointly with
Prof. JA Bryant, University of Exeter.
COUNCIL MEMBER -SOCIETY FOR EXPERIMENTAL BIOLOGY (SEB). Elected 2002-2007.
Equivalent to Board of directors.
7. EDUCATION OFFICER -SOCIETY FOR EXPERIMENTAL BIOLOGY (SEB) 1999- 2005.
for School of Biosciences, University of Birmingham. Involved in SEB Schools programme session at the
Annual SEB meeting in Exeter.
8. EDITORIAL WORK FOR INTERNATIONAL JOURNALS

Review Editorial Board of Frontiers in Plant Physiology Jan 2011-current
www.frontiersin.org This is a newly launched journal system which extracts and distills the most
outstanding research. Authors of the most impactful Original Research Articles are invited to write a
Focused Review, centred on original discoveries, but placed in a broader context for the general
reader in the field with the goal of conveying the discovery to other specialists in the field.

Associate Editor for Journal of Experimental Botany, Oct 2011-current.
The Journal of Experimental Botany (Impact factor: 4.271) publishes high-quality primary research
and review papers in the plant sciences. These papers cover a range of disciplines from molecular
and cellular physiology and biochemistry through whole plant physiology to community physiology.
20

EDITORIAL ADVISORY BOARD MEMBER 2008-2013
The Open Plant Science Journal (new Open Access online journal)

EDITORIAL BOARD MEMBER 2007-2011
Editorial board of Plant and Cell Physiology (Japanese Society of Plant Physiologists).
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EDITORIAL BOARD MEMBER 2006-current
Editorial board of Sexual Plant Reproduction, published by Springer.
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EDITORIAL BOARD MEMBER 2005-2011
Associate Editor for a new Journal being launched in 2006: Plant Signalling and Behavior, published
by Landes Bioscience.
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PLANT JOURNAL ADVISORY BOARD MEMBER (1997-2004).
EXPERT OPINION
1. Professorial promotions committee: Member of the University's
Promotions Committee, Oxford Brookes University, UK. 2011-2012.
Professorial
2. Active participation in tenure reviews or professorial appointments at major overseas
universities. I have been approached to write letters of evaluation in the capacity of an
external independent international expert. I have indicated my expert opinion on
people who are up for promotion, being recruited to new Chairs, candidates for
National Awards.
3. International Advisory Board member for the Federation of European Societies of Plant
Biology (FESPB) Board Member for the biennial Congress in 2006, Lyon, France.
4. International Advisory Board for the IASPRR “Plants For Food”, Melbourne, Australia.
5. MANUSCRIPTS PRE-SUBMISSION ENQUIRIES
I am consulted by journals like Nature, Science, Current Biology about whether pre-submisison
enquiries should be followed up or submissions sent out.
6. GRANT PROPOSALS REFEREEING
I routinely referee grant applications a year for various agencies including: BBSRC, NSF, USDA,
Human Frontier Science Program (HFSP), Katholieke Universiteit Leuven, Belgium.
7. MANUSCRIPTS REFEREEING I routinely referee 1 manuscript a week for journals including:
Nature, Science, Current Biology, Nature Cell Biology, Trends in Cell Biology, Journal of Cell Science,
The Plant Cell, Plant Journal, Plant Physiology, Plant Biology, Planta, New Phytologist, Plant Molecular
Biology, Annals of Botany, Journal of Experimental Biology, FEBs Letters, Sexual Plant Reproduction,
Plant Biosystems, Euphytica, Heredity, Kluwer publishers.
8. European Technology Platform “Plants for the Future”: Involved in UK Consultation Group
discussions, organized by Mike Bevan (JIC). Consultations on Stakeholders Proposal for a Strategic
Research Agenda: inputting into the policy forming document for European Technology Platform
“Plants
for
the
Future”,
wrt
Draft
Action
Plan.
London,
December
2005.
http://www.epsoweb.org/Catalog/TP/index.htm
9. External PhD examiner: University of Manchester, Exeter, Glasgow
PUBLIC UNDERSTANDING OF SCIENCE & SCHOOLS EVENTS :
I have had a long-standing involvement in widening participation and understanding of science, and have liased
with BBSRC for many years in events for schools and the public, to promote science to a wider audience.
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1. ORGANIZED A PUBLIC EVENT “FLOWER POWER”, Victoria rooms, Bristol, Aug 2010
I liaised with BBSRC Publicity and the to organize an evening interactive events in association with an
international meeting: the IASPRR Congress. This event comprised a series of 6 large displays
illustrating the importance of plant reproduction and how scientists are working to use knowledge for
applied work. Several hundred people attended this event, which was opened by the Lord Mayor of
Bristol and was manned by myself and several scientists involved in the conference.
2. JUDGE AT THE 2005 CELEBRATION OF YOUNG SCIENTISTS AND ENGINEERS
The annual Regional Finals of the BA CREST Awards; 1st July 2005, at Think Tank, Millenium Point,
Birmingham.
3. Special exhibition/activities, Feb. 2006: I liased with Kenny Webster, the Science Officer at
Thinktank, Millenium Point, Curzon Street, Birmingham, to organize a 1 day stand at the BBSRC “DNA
in The Garden Exhibition”, February 2006, for the general public during half-term week. This was
manned by myself and members of my laboratory.
4.
Schools visit March 2006: I was invited to visit John Cleveland College, Hinckley, Leicestershire,
which specializes in Science teaching. I presented a lecture to 6th Formers on plant reproduction.
5.
Participated in event at Birmingham University organized by BDI consultants: “Getting Women on
Track” event aimed at encouraging school girls to go into science careers. Panel member: role
model on Women in Education.
6. BBSRC SCHOOLS LIAISON SCHEME 1998-1999
Participated in a Liaison project between the University and Kings Norton J&I School.
7. BBSRC REGIONAL COORDINATOR OF THE BBSRC Schools Liaison Scheme WEST
MIDLANDS CLUSTER Sept 1999- 2003. This involved: Liaising with contacts at BBSRC and
SATRO. Encouraging staff and students in Biosciences to participate in the scheme. Compiling
information on schools participating. Coordinating matching scientists with schools. Organising
meetings, writing Newsletters, disseminating information to the Group. Reporting back to BBSRC on
the Projects. Publicity for the Programme. Managing the budget for the projects.
8. Presentation to scientists taking part in BBSRC “Communicating with the Public” workshop. Spoke
about the Primary Schools liaison scheme (Wellesbourne, Feb 2000)
9. Presentation to school teachers at the annual ASE meeting (Liverpool, Jan 2002) about the Schools
liaison scheme and experiences with it.
10. INTERACTIONS WITH THE MEDIA
I contributed to a commentary article in Science magazine’s News website, ScienceNOW, entitled "How
Plants Avoid Incest" (2006).
My research has attracted considerable media attention. Examples include:
 I contributed to writing a press release entitled "Sexual Frustration: programmed cell death prevents
plant inbreeding" for the BBSRC website.
 I had a phone interview with the science correspondent from the leading national daily newspaper in
Brazil (Folha de Sao Paolo) and an article describing my work was published in this newspaper.
 I had an Email-based interview with the German daily scientific News service, Wissenschaft
Online, with parts of this interview published in the Spectrum-Direkt and AgBiotech Net.

My research was discussed in popular layman's terms in an article in Science magazines News
website, ScienceNOW (May 2004).

I was interviewed and comments included in article in online article: Science-NOW news section
article: “How plants avoid Incest” (2006)
 I liased with the science writer for JCB’s highlight “In this Issue” section.
 The journal, Cell, ran a “Leading Edge” article, highlighting my work (2006). I liaised with them over
editing the summary paragraph and provided images.
 I contributed to writing a press release for the BBSRC website: “Inbreeding –there’s a stigma
attached!” (2007).
 Full page article in BBSRC’s BUSINESS magazine, April 2007.
 Front cover picture feature on research in my lab, and I contributed to the writing of article (“to bee or
not to be…”) in BUZZ magazine (UoB & to the general public) & inside feature article May 2007.
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 I contributed writing a press release for the BBSRC website: “Scientists solve poppy puzzle with
new gene discovery” (2009) and an article to BUZZ magazine in Aug 2009.
CONTRIBUTED DISCUSSION to the Change Exchange. Blog created to celebrate National Science and
Engineering Week (March 2009). Blog on Agriculture & food, specifically on genetically modified food- are we
making foods that may harm us? Responses to an anti-GM Greenpeace supporter.
ORGANIZATION OF INTERNATIONAL CONFERENCES
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Co-organiser IPMB Symposium in St Louis, USA (Oct. 2009). " Molecular/ cellular control of
pollen tube growth" joint with Bruce McClure.
Co-organiser SYMPOSIUM on Reproductive Biology. Swansea, April 2002.
organiser of an International Meeting: Pollen-stigma interactions, St Catherines,
University of Oxford, July 1999. ~150 delegates.
Co-organiser of Society for Experimental Biology (SEB) International symposium
EDITORIAL WORK
 EDITED BOOK: Invited by Springer-Verlag to organize an edited a book on Self-incompatibility,
comprising 14 chapters: V.E. Franklin-Tong (ed.) Self-Incompatibility in Flowering Plants –
Evolution, Diversity, and Mechanisms. Publ. Springer-Verlag Berlin Heidelberg 2008. This is the first
monograph on this topic for 30 years.
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EDITORIAL WORK ON J. EXP. BOT. SPECIAL ISSUE (2011).
I was co-editor of the Special Issue of J. Exp. Bot. that resulted from IASPRR meeting in Bristol, 2010.
EDITORIAL WORK ON J. EXP. BOT. SPECIAL ISSUE (2010).
I was co-editor of the Special Issue of J. Exp. Bot. that resulted from the SEB symposium.
EDITORIAL WORK ON J. EXP. BOT. SPECIAL ISSUE (2003). I was editor of the Special Issue
of J. Exp. Bot. that resulted from the SEB symposium. I was responsible for overseeing refereeing,
editing and acceptance of all the 18 papers in this Issue, and for cover images.
EDITORIAL WORK ON ANNALS OF BOTANY SPECIAL ISSUE. 1999.
Other measures of recognition
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Short-listed and interviewed for the Sherardian Chair, University of Oxford (Feb 2008)
Part of the Birmingham “Heroes” campaign autumn 2010 –poster campaign to highlight important
research ongoing at Birmingham University on city trains, at the airport, several magazines and London
Underground.
Short-listed for inaugural Founders Award: Joseph Chamberlain award for academic advancement,
July 2010.
Selected by HoD for my research achievements to be mentioned by VC in December 2010 degree
ceremony speech
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