Li-Cor Biosciences Odyssey® Imaging System
The Odyssey Imaging System offers exceptional versatility for protein and other target imaging compared to other
available systems. This system uses infrared and near-infrared fluorescent probes that possess a very wide linear
dynamic range with two channels so that two separate targets can be visualized and quantified simultaneously. The
following description provides additional explanations of the advantages that this system provides for performing
protein and other target analysis.
1.
Wide Linear Range – Through the use of fluorescently labeled antibodies rather than an enzymatic reaction,
the Odyssey provides a broad linear dynamic range to accurately detect strong and weak bands on the same
Western blot.
2.
High Sensitivity – The use of the infrared spectrum provides for equal to or better sensitivity than
chemiluminescence because autofluorescence and light scatter is dramatically reduced. This results in the
cleanest background, highest signal-to-noise ratios, and best detection sensitivity available with a fluorescent
system.
3.
Direct Detection – When using the Odyssey system, there is no film, darkroom, or messy substrates. The dyes
are stable on the membranes for years (as long as they are properly stored).
4.
Flexible System with Many Applications
Applications include:
Coomassie Gels
DNA Gel Staining
ELISA/FLISA
EMSA/Gel Shift Assay
Glycoprotein Detection
In-Cell Western Assay
In-Gel Western Assay
Northern Blot
Protein Array
Quantitative Western Blot
Reporter Gene Assay
Reverse Phase Array
RNAi Screens
Small Animal Imaging (uses the MousePod but also see Pearl Imager)
Southern Blot
Tissue Section Imaging
Transcription Factor Assay
5.
Multiple user capability --The software provided for acquisition and analysis is site licensed at no cost and
with the extended warranty lifetime upgrades are provided at no cost.
The instrument is currently housed in Rm. 6S11 in the Brody Medical Sciences building. If you are interested in
information regarding this item of equipment and its use, please contact Dr. David A. Taylor or Ms. Kathleen Thayne in
the Department of Pharmacology and Toxicology.