TECHNIQUES IN MOLECULAR BIOLOGY SYLLABUS
Biol 319 – Fall 2010
Instructor:
Office:
Phone:
E-mail:
Website:
Dr. Diane Caporale
437 TNR Building
(715) 346-3922
dcaporal@uwsp.edu
http://www.uwsp.edu/biology/faculty/DCaporal
Office Hours:
Mon & Wed: 1-2 pm
(or by appointment)
Required Lab Manual: Caporale, D. 2010. Techniques in Molecular Biology: A Laboratory Manual.
Department of Biology, UW -Stevens Point. For purchase in UWSP Bookstore. ($3.80)
Required Laboratory Notebook: Purchase a small notebook with lined (or graph) paper. This will be
used to take notes in lab to help prepare for your lab reports.
Class Meetings:
Section 1: Tues 10:00am – 12:50pm; Sci B212
Section 2: Thurs 10:00am – 12:50 pm; Sci B212
Course Objectives: 1) to understand basic molecular approaches to answer a variety of biological
questions, and 2) to gain experience in using basic molecular laboratory techniques.
Grading: (Lab #3 is omitted this semester)
Assignment
% Points
Lab 1: Gel electrophoresis
Lab 2: Southern Blot: A paternity case
Lab 3: Tick Flagging Participation
Lab 4: DNA isolate & quantify
Lab 5: PCR: Detection of a pathogen
10
10
5
10
Assignment
Lab 6: DNA sequencing
Lab 7: RAPD fingerprinting
Lab 8: AFLP
Laboratory Participation
% Points
10
15
15
15
10
Grading Scale: The grading scale above is firm. There will be no borderline grades. If you attend and
participate in class, read your manual, hand in your lab reports on time and ask questions, then you’ll
find success in accumulating points. The lab participation grade refers to your attendance and effort
during each lab meeting. Guidelines on how to write a lab report are in the lab manual. Late reports
are subject to 10% off each day late.
Attendance Policy: Students who must miss a lab due to religious observances or participation in
university sanctioned events should notify me within the first 3 weeks of the beginning of class, so
makeup arrangements can be made. The only other valid excuses for missing a lab or deadline
are death in the family, violent illness, or accident. In such cases: (1) you must provide evidence
of some kind (e.g. note from health center), and (2) you must reschedule within 24 hrs after the
date of the deadline.
Academic Misconduct: You are responsible for the honest completion and representation of your
work and for the respect of others’ academic endeavors. Any act of cheating, plagiarism, or
academic misconduct is subject to the penalties outlined in UWS Chapter 14.
Please refer to this link for more information:
http://www.uwsp.edu/centers/rights/RRBOOKLET8-2005-06.pdf
TECHNIQUES IN MOLECULAR BIOLOGY SCHEDULE
Week
Date
Topic
Lab Investigation
& Pages
2
Sept
7, 9
Intro, Prepare 1X TAE buffer
Pour 2 Gels
3
14, 16
4
21, 23
Gel Electrophoresis Lab
Southern Blot Lab
Southern Blot Lab – detect DNA
ii-iv
1: 1-4
2:14
1: 5-7
2: 8-17
2: 17-19
5
28, 30
NO LAB
6
Oct 5
Flagging in Stockton
handout
7
7
12, 14
Flagging in Schmeeckle Reserve
1st DNA Isolation & Quantification Lab
4: 31-35
19, 21
PCR Lab - Amplification by RealTime-PCR /
Pour Gel
2nd DNA Isolation
8
9
26, 28
10
Nov
2, 4
11
9, 11
12
16, 18
13
23, 25
14
30,
Dec 2
15
7, 9
16
14
Run PCR Gel & analyze RT-PCR results/
Purify PCR Products/ Cycle-Sequence
Quantify 2nd DNA
Purification of Cycle-Seq Products/
DNA Sequence
AFLP Lab - Digest & Ligate
DNA Sequence Analysis - Edit Sequences
AFLP Preselective Amplification
RAPD Lab - Amplification
DNA Sequence Analysis - Blast Search/
Sequence Alignment
RAPD Lab - Pour & Run Agarose Gel /
RAPD Genescan
AFLP Selective Amplification
5: 36-43
33
5: 43-45
6a: 46-51
34-35
6b: 52-53
6b: 54-58
8: 73-78
6c: 59-61
8: 79-80
7: 62-65
6c: 59-61
Gel Lab #1
Southern Blot Lab
#2
DNA Quant lab
#4
PCR lab
#5
7: 66-69
8: 80-82
DNA Sequence Lab
#6 (a,b,c)
Thanksgiving Break!!!!
lab due Mon, Nov 23 by Noon
drop off at TNR 437 - Dr. C's office
RAPD Analysis - Edit DNA Fingerprints
(Genemapper) /
Create 01 Matrix(Geneious)
AFLP Lab - Pour & Run Gel/AFLP Genescan
AFLP Analysis
(Genescan or GeneMapper)
due Friday, Dec 18 by Noon
drop off at TNR 437 - Dr. C's office
Assignment Due
7: 70-72
8: 82-84
8: 84
70-71
Lab open Tuesday to
finish AFLP analysis
RAPD Lab #7
AFLP Lab #8