Laboratory Standard Operating Procedure
(For the Use of Hazardous Materials or Equipment)
Name of Procedure: High Performance Liquid Chromatography - HPLC___________
Prepared by: ________________________
Revision Date: ___________
LOCATION- This procedure may be performed in the following location(s):
Building :
Room #:
HAZARDS- The following materials and equipment associated with this procedure present exposure or
physical health hazards. The following hazards require safety precautions:
ACETONITRILE (CAS # 75-05-8)- May be fatal if swallowed, inhaled or absorbed through the skin. Affects
cardiovascular system, central nervous system, liver and kidneys. Flammable liquid and vapor. May cause irritation
to skin, eyes, and respiratory tract. Review MSDS prior to working with Acetonitrile.
METHANOL (CAS # 67-56-1) – Vapor harmful. May be fatal or cause blindness if swallowed. Harmful if
inhaled or absorbed through the skin. Cannot be made non-poisonous. Flammable liquid and vapor. Causes irritation
to skin, eyes, and respiratory tract. Affects central nervous system and liver . Review MSDS prior to working with
Methanol.
ENGINEERING CONTROLS: Prior to performing this procedure, the following safety equipment must
be accessible and ready for use: (ex. Chemical fume hood, biological safety cabinet, laminar flow hood)
Containers should be capped when not in use.
PROTECTIVE EQUIPMENT- Prior to performing this procedure, the following personal protective
equipment (PPE) must be obtained and ready for use: (ex. Acid resistant gloves, safety eyewear, lab coat,
chemical splash apron)
Nitrile gloves are suitable for handling containers and cleaning up small spills of Acetontrile and Methanol. If
accidental contact occurs, remove and discard contaminated gloves immediately. Break though may occur after
prolonged exposure.
Safety glasses and lab coat must be worn when performing all tasks associated preparing and running HPLC
analysis. Wear chemical splash goggles when there is a splash hazard (when pouring chemicals).
WASTE DISPOSAL- This procedure will result in the following regulated waste, which must be
disposed of in compliance with the REHS Hazardous Waste Policy:
Acetonitrile and Methanol waste must be collected and disposed of via REHS.
ACCIDENTAL SPILL- In the event of a spill of hazardous materials, follow these procedures:
Small Spill
ASSESS spill size, chemical type and available
cleanup equipment.
EVACUATE the immediate area.
WARN other personnel.
WEAR appropriate PPE.
CLEAN UP spill using appropriate spill kit if trained
to do so.
CONTAIN spilled material in a sturdy plastic bag or
sealed drum. Apply a hazardous waste label and
contact REHS for disposal.
Large Spill
ATTEND to injured persons, seek appropriate
medical attention.
EVACUATE area/building.
EXTINGUISH ignition and heat sources.
PREVENT spill from spreading.
CONTACT REHS for assistance. After hours call
Campus Police.
FILE a formal incident report
PRIOR APPROVAL- This procedure is considered hazardous enough to warrant prior approval from
the PI or department safety coordinator: YES/NO
NO