Standard Operating Procedures for

UC Irvine Environmental Health & Safety: Example
Standard Operating Procedures for
Hazardous Chemicals and Particularly Hazardous Substances
#1 Process
Topical Application of DMBA. All users must read the Material Safety Data Sheet ( ) prior to
handling this material. Additional safety information can also be obtained from Enter CAS number: 57-97-6
#2 Hazardous Chemicals/Class of Hazardous Chemicals
Dimethylbenzanthracene CAS No. 57-97-6. This chemical is an experimental carcinogen, neoplastigen, tumorigen,
and teratogen. Poison by ingestion, intravenous, subcutaneous, intraperitoneal, and intratracheal route.
#3 PPE - Personal protective Equipment
Wear safety glasses when handling the material. When the chemical is handled as dry material, an N95 HEPA filter
respirator must be worn. Contact EH&S for procedures on how to obtain a respirator. MSDS recommends the use
of a 0.38 mm thick nitrile gloves (Edmont 37-155 ). .
#4 Engineering/Ventilation Controls
Use in a “Designated Area” only such as a fume hood. Decontaminate all surfaces after a spill. See spill and
decontamination procedures below.
#5 Special Handling Procedures and Storage Requirements
Protect material from exposure to light. Keep away from oxidizing materials and store under refrigerated
Spill and Accident Procedures
Small Spills: Do not attempt clean-up if you feel unsure of your ability to do so or if you perceive the risk to be
greater than normal laboratory operations. Absorb incidental spills with absorbent pads. Collect and submit for
waste disposal by EH&S.
Large Spills: Notify others in area. Evacuate room/immediate area. If splashed on an individual or in eyes flush for
15 minutes with copious quantities of water. Call EH&S for HazMat response. Post area and prevent unnecessary
entry until EH&S response team arrives. Provide assistance to EH&S as requested.
If a spill occurs, remove all sources of ignition. Dampen the solid spill of material with acetone and transfer the
material to a labeled “Hazardous Waste” container. Use absorbent paper dampened with acetone to pick-up
remaining material. Seal contaminated clothing and absorbent paper in a vapor tight plastic bag for hazardous waste
#7 Waste Disposal
Dispose contaminated material as hazardous waste.
#8 Approval Required
Approval required from PI.
#9 Decontamination Procedures
Solvent wash all contaminated surfaces with acetone followed by washing with soap and water solution. Do not reenter the contaminated area if large spill occurs.
#10 Designated Area
Handle dry powders inside a fume hood.
For more information relevant to completing this form, review thoroughly Chemical Hygiene Plan: Section 5.
Training Acknowledgement: I have read, asked questions, and understand the hazards of and safe working
procedures for the activity/materials described herein.
UC Irvine Environmental Health & Safety: Example
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