MATERIALS AND METHODS
Dicer processing of the bipartite pRNA/siRNA complex
The procedure for in vitro Dicer processing assay has been reported previously(23).
The 5’-end [32P] labeled bipartite pRNA/siRNA chimera or the intact pRNA/siRNA
chimera was treated with the recombinant Dicer (Genlantis/Gene Therapy Systems, San
Diego, CA) and separated by 16% Urea/PAGE gel for subsequent autoradiography.
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