Chapter 7
Genetic Transfer and Mapping in Bacteria and Bacteriophages
Study Outline
I. Genetic Transfer and Mapping in Bacteria a
A. Bacteria can transfer genetic material during conjugation
B. Hfr strains contain an F factor integrated into the bacterial chromosome
C. Hfr strains can transfer a portion of the bacterial chromosome to recipient cells
D. Conjugation experiments can map genes along the E. coli chromosome
E. A genetic map of the E. coli chromosome has been obtained from many conjugation
studies
F. Bacteria may contain different types of plasmids
G. Bacteriophages transfer genetic material from one bacterial cell to another via
transduction
H. Cotransduction can be used to map genes that are within 2 minutes of each other
I. Bacteria can also transfer genetic material by transformation
J. Bacteria may acquire new genes by horizontal gene transfer
II. Intragenic Mapping in Bacteriophages
A. Mutations in viral genes can alter plaque morphology
B. A complementation test can reveal if mutations are in the same gene or in different genes
C. Intragenic maps were constructed using data from a recombinational analysis of mutants
within the rII region
D. Deletion mapping can be used to localize many rII mutations to specific regions in the
rIIA or rIIB genes
E. Intragenic mapping experiments provided insight into the relationship between traits and
molecular genetics