Curriculum Vitae
Bruce Kelder, PhD
Scientist II
Edison Biotechnology Institute
206B Konneker Research Laboratories
Ohio University
Athens, OH 45701
Telephone: (740) 593-4605
Fax: (740) 593-4795
Email: kelder@ohio.edu
Academic Preparation
Ohio University, Athens, OH
Field of Study: Molecular and Cellular Biology
Doctor of Philosophy, 1995
University of Maryland, College Park, MD
Field of Study: Zoology
Bachelor of Science, 1980
Professional Work Experience
1979-1980
Undergraduate Research Assistant, The National Institutes of Health,
Bethesda, MD.
1981-1985
Assistant Scientist, Roche Institute of Molecular Biology, Nutley, NJ.
1985-1987
Staff Biochemist, Dept. of Animal Drug Discovery, Merck Sharp & Dohme
Research Laboratories, Rahway, NJ.
1987-present Scientist II, Edison Biotechnology Institute, Ohio University, Athens, OH.
Other Experience and Professional Memberships
2008- present
Member, The Endocrine Society
Scholarly and Creative Accomplishments
US Patent Application No: 20070111933 (Kopchick JJ, Kelder B, Boyce K, Kriete A)
“Diagnosis and treatment methods related to aging, especially of liver” (May 17, 2007).
US Patent Application No: 20030159164 (Kopchick JJ, Kelder B, Yung-Sheng H, Kirchner S,
Mukerji P) Compositions and methods for the synthesis of fatty acids, their derivatives and
downstream products (August 21, 2003).
Book Chapters
Bruce Kelder. Book Chapter: Reproduction: Laron Syndrome-From Man to Mouse, SpringerVerlag Chapter 58 p507-512, 2011.
Kelder B, Rashidbaigi A, Pestka S. A sandwich radioimmunoassay for human IFN"Methods in Enzymology" (Pestka, S., ed.) Academic Press Inc., Vol. 19, pp. 582-587, 1986.
Refereed Journal Articles
List EO, Berryman DE, Funk K, Gosney E, Jara A, Kelder B, Wang X†, Kutz L, Troike K†,
Lozier N, Lubbers E†, Zhang H†, Vesel C†, Junnila R, Frank SJ, Masternak M, Bartke A,
Kopchick JJ. Fat-specific deletion of the growth hormone receptor results in obesity with no
alteration in glucose homeostasis. Molecular Endocrinology (in press, accepted December 10,
2012).
Cruz-Topete D, List EO, Okada S, Kelder B, Kopchick JJ. Proteomic changes in the heart of
diet-induced pre-diabetic mice. J. Proteomics May 74(5):716-27, 2011.
List EO, Sackmann-Sala L, Berryman DE, Funk K, Kelder B, Gosney ES, Okada S, Ding J,
Cruz-Topete D, Kopchick JJ. Endocrine parameters and phenotypes of the growth hormone
receptor gene disrupted (GHR-/-) mouse. Endocr Rev. Jun:32(3):356-86, 2011.
Rouet V, Bogorad RL, Kayser C, Kessal K, Genestie C, Bardier A, Grattan DR, Kelder B,
Kopchick JJ, Kelly PA, Goffin V. Local prolactin is a target to prevene expansion of basal/stem
cells in prostate tumors. Proc. Natl. Acad. Sci. USA Aug 107(34):15199-204, 2011.
Longo KA, Govek EK, Nolan A, McDonagh T, Charoenthongtrakul S, Giuliana DJ, Morgan K,
Hixo J, Zhou C, Kelder B, Kopchick JJ, Saunders JO, Navia M, Curtis R, DiStefano PS, Geddes
BJ. Pharmacologic inhibition of ghrelin receptor signaling is insulin sparing and promotes insulin
sensitivity. J Pharmacol Exp Ther. 2011 Oct;339(1):115-24. Epub 2011 Jul 20.
Longo KA, Berryman DE, Kelder B, Charoenthongtrakul S, Distefano PS, Geddes BJ, Kopchick
JJ. Daily energy balance in growth hormone receptor/binding protein (GHR -/-) gene-disrupted
mice is achieved through an increase in dark-phase energy efficiency. Growth Horm IGF Res.
20:73-79, 2010.
List EO, Palmer AJ, Berryman DE, Bower B, Kelder B, Kopchick JJ. Growth hormone
improves body composition, fasting blood glucose, glucose tolerance and liver triacylglycerol in
a mouse model of diet-induced obesity and type 2 diabetes. Diabetologia Aug;52(8):1647-55,
2009.
Kelder B, Berryman DE, Clark R, Li A, List EO, Kopchick JJ. CIDE-A gene expression is
decreased in white adipose tissue of growth hormone receptor/binding protein gene disrupted
mice and with high-fat feeding of normal mice. Growth Horm IGF Res. Aug;17(4):346-351,
2007.
Kelder B, Boyce K, Kriete A, Clark R, Berryman DE, Nagatomi S, List EO, Braughler M,
Kopchick J.J. CIDE-A is expressed in liver of old mice and in type 2 diabetic mouse liver
exhibiting steatosis. Comp. Hepatol. May 1;6:4, 2007.
List E.O., Berryman D.E., Palmer A., Gosney E., Okada S., Kelder B., Lichtenberg J., Welch
L.R., and Kopchick J.J. Application of bioinformatics and scalable computing to perform
proteomic analysis of stomach tissue from diabetic mice. Scalable Computing: Practice and
Experience 8(2):173-183, 2007.
List E.O., Berryman D.E., Palmer A., Kelder B., Qiu L., Okada S., and Kopchick J.J.. Analysis
of mouse skin reveals proteins that are altered in a diet-induced diabetic state: a new method for
detection of type 2 diabetes. Proteomics Mar 27;7(7):1140-1149, 2007.
Manhes C, Kayser C, Bertheau P, Kelder B., Kopchick JJ, Kelly PA, Touraine P and Goffin V.
Local over-expression of prolactin in differentiating mouse mammary gland induces functional
defects and benign lesions, but no carcinoma. J Endocrinol. 190(2):271-85, 2006.
Boyce, K., Kriete, A., Nagatomi, S., Kelder, B., Coschigano, K. and Kopchick, J.J. Phenotypical
enrichment strategies for microarray data analysis applied in a type II diabetes study. J.
Integrative Biology, 9(3):252-266, 2005.
Leonard, A.E., Kelder, B., Gobik, E.G., Chuang L., Lewis, C.J., Kopchick, J.J., Mukerji, P. and
Huang, Y-S. Identification and expression of mammalian long-chain PUFA elongation enzymes.
Lipids 37:733-740, 2002.
Li, Y., Kelder, B. and Kopchick, J.J. Identification, isolation and cloning of growth hormone
(GH)-inducible interscapular borwn adipose complementary deoxyribonucleic acid from GH
antagonist mice. Endocrinology 142(7):2937-2945, 2001.
Kelder, B., Erney, R., Kopchick, J.J., Cummings, R. and Prieto, P. Glycoconjugates in human
and transgenic animal milk. Adv Exp Med Biol. 501:269-78, 2001.
Kelder, B., Mukerji, P., Kirchner, S., Hovanec, G., Leonard, A.E., Chuang, L-T., Kopchick, J.J.
and Huang, Y-S. Expression of fungal desaturase genes in cultured mammalian cells. Mol. Cell.
Biochem. 21:7-11, 2001.
Das, T., Johns, P.W., Goffin, V., Kelly, P., Kelder, B., Kopchick, J.J., Buxton, K. and Mukerji, P.
High-level expression of biologically active human prolactin from recombinant baculovirus in
insect cells. Protein Expr. Purif. 20:265-273, 2000.
Leonard, A.E., Kelder, B., Bobik, E.G., Chuang, L.T., Parker-Barnes, M., Thurmond, J.M.,
Kroeger, P.E., Kopchick, J.J., Huang, Y.S. and Mukerji, P. Identification and expression of
human delta 5-desaturase gene. Biochem. J. 347:719-724, 2000.
Prieto, P.A., Kopchick, J.J. and Kelder, B. Transgenic animals and nutrition research. J. Nutri.
Biochem. 10:682-695, 1999.
Tarapore, P., Richmond, C., Zheng, G., Cohen, S.B., Kelder, B., Kopchick, J.J., Kruse, U.,
Sippel, A.E., Colmenares, C. and Stavnezer, E. DNA binding and transcriptional activation by
the Ski oncoprotein mediated by interaction with NFI. Nucl. Acids Res. 25(19):3895-3903,
1997.
Kelder, B., Stavenezer, E. and Kopchick, J.J. Production, characterization and functional
activities of v-ski in cultured cells. Gene, 202:15-21, 1997.
Tuck, M., James, C. B. L., Kelder, B. and Kopchick, J. J. Elevation of internal 6-methyladenine
mRNA transferase activity after cellular transfromation. Cancer Letters, 103:107-113, 1996.
Kopchick, J.J., Jura, J., Mukerji, P. and Kelder, B. Transgenic technology as it applies to animal
agriculture. Biotechnologia, 2:33:31-35, 1996.
Prieto, P.A., Mukerji, P., Kelder, B., Erney, R., Gonzalez, D., Yun, J.S., Smith, D.F., Moremen,
K.W., Nardelli, C., Pierce, M., Li, Y., Chen, X., Wagner, T.E., Cummings, R.D. and Kopchick,
J.J. Remodeling of mouse milk glycoconjugates by transgenic expression of a human
glycosyltransferase. J. Biol. Chem. 270:49:29515-29519, 1995.
Wang, X., Souza, S.C., Kelder, B., Cioffi, J.A. and Kopchick, J.J. A 40-amino acid segment of
the growth hormone receptor cytoplasmic domain is essential for GH-induced tyrosinephosphorylated cytosolic proteins. J. Biol. Chem. 270:11:6261-6266, 1995.
Harding, P.A., Wang, X.Z., Kelder, B., Souza, S. and Kopchick, J.J. In vitro mutagenesis of
Asn-linked glycosylation sites in the porcine growth hormone receptor. Mol. and Cell. Endo.
106:171-180, 1994.
Campbell, R.M., Chen, W.Y., Wiehl, P., Kelder, B., Kopchick, J.J. and Scanes, C.G. A growth
hormone (GH) analog that antagonizes the lipolytic effect but retains full insulin-like
(antilipolytic) activity of GH. Proc. Soc. Exp. Biol. and Med., 203:311-316, 1993.
Wang, X.Z., Cioffi, J.A., Kelder, B., Harding, P., Chen, W. and Kopchick, J.J. Expression of
Porcine Growth Hormone Receptor (pGHR) cDNA in Mouse L Cells. Molecular and Cellular
Endocrinology, 94:89-96, 1993.
Okada, S., Chen, W.Y., Wiehl, P., Kelder, B., Goodman, H.M., Guller, S. Sonenberg, M. and
Kopchick, J.J. A growth hormone (GH) analog can antagonize the ability of native GH to
promote differentiation of 3T3-F442A preadipocytes and to stimulate insulin-like and lipolytic
activities in primary rat adipocytes. Endo. 130(4):2284-2290, 1992.
McAndrew, S.J., Chen, N.Y., Kelder, B., Cioffi, J.A. and Kopchick, J.J. Effects of leucine
analog on growth hormone processing and secretion by cultured cells. Journal of Bio. Chem.,
266:15016-15020, 1991.
Kelder, B., Chen, H. and Kopchick, J.J. Activation of the mouse metallothionein-I promoter in
transiently transfected avian cells. Gene 76:75-80, 1989.
Martin-Gallardo, A., Montoya-Zavala, M., Kelder, B., Taylor, J., Chen, H., Leung, F. and
Kopchick, J.J. A comparison of bGH expression in mouse L cells directed by the Moloney
murine leukemia virus long terminal repeat, the simian virus 40 early or cytomegalovirus
immediate early promotors. Gene, 70:151-156, 1988.
Cascieri, M., Hayes, N., Kelder, B., Kopchick, J.J., Chicchi, G., Slater, E. and Bayne, M.
Inability of a mouse cell line transformed to produce biologically active recombinant human
insulin-like growth factor I (IGF-I) to respond to exogenously added IGF-I. Endocrinology
122:1314-1320, 1988.
Bayne, M.L., Cascieri, M.A., Kelder, B., Applebaum, J., Chicchi, G., Shapiro, J., Pasleau, F. and
Kopchick, J.J. Expression of a synthetic gene encoding human insulin-like growth factor I in
cultured mouse fibroblasts. Proc. Natl. Acad. Sci., USA, 84: 2638-2642, 1987.
Daugherty, B., Martin-Zanca, D., Kelder, B., Collier, K., Seamans, T.C., Hotta, K. and Pestka, S.
Isolation and bacterial expression of a murine alpha leukocyte interferon gene. J. Interferon Res.
4:635-643, 1984.
Ortaldo, J.R., Herberman, R.B., Harvey, C., Oscheroff, P., Pan, Y.-C.E., Kelder, B. and Pestka,
S. A species of human -interferon that lacks the ability to boost human natural killer activity.
Proc. Natl. Acad. Sci. USA 81:4926-4929, 1984.
Pestka, S., Kelder, B., Tarnowski, D.K. and Tarnowski, S.J. Specific immunoassay for protein
dimers, trimers, and higher oligomers. Anal. Biochem., 132:328-333, 1983.
Pestka, S., Kelder, B., Familletti, P.C., Moschera, J.A., Crowl, R. and Kempner, E.S. Molecular
weight of the functional unit of human leukocyte, fibroblast, and immune interferons. J. Biol.
Chem., 258:9706-9709, 1983.
Pestka, S., Kelder, B., Langer, J.A. and Staehelin, T. Monoclonal antibodies can discriminate
between some active and inactive forms of leukocyte interferon. Arch. Biochem. Biophys.,
224:111-116,1983.
Ortaldo, J.R., Mason, A., Rehberg, E., Moschera, J., Kelder, B., Pestka, S. and Herberman, R.B.
Effects of recombinant and hybrid recombinant human leukocyte interferons on cytotoxic activity
of natural killer cells. J. Biol. Chem., 258:15011-15015, 1983.
Rehberg, E., Kelder, B., Hoal, E.G. and Pestka, S. Specific molecular activities of recombinant
and hybrid leukocyte interferons. J. Biol. Chem., 257:11497-11502, 1982.
Pestka, S., Evinger, M., Maeda, S., Rehberg, E., Familletti, P.C. and Kelder, B. Biological
properties of natural and recombinant interferons. Tex. Rep. Biol. Med., 41:31-36, 1982.
Poster Presentations/Published Abstracts
Tanda H, Kelder B, Berryman DE, Okada S, Tarnowski M, List EO, Kopchick JJ. Effects of
Transgenic Human CIDE-A Expression on Body Composition and Lipid Accumulation in Mice.
The Endocrine Society 92st Annual Meeting, San Diego, CA, June 19-22, 2010.
Cruz-Topete D, List EO, Okada S, Kelder B, Kopchick JJ Identification of cardiac biomarkers
in a mouse model of type 2 diabetes The Endocrine Society 92st Annual Meeting, San Diego,
CA, June 19-22, 2010.
Kelder B, Berryman DE, Okada S, List EO, Tarnowski M, Kopchick JJ. Generation and
Characterization of Transgenic Mice Expressing the Human CIDE-A cDNA. P2-391, The
Endocrine Society 91st Annual Meeting, Washington DC. June 10-13, 2009.
Kelder B, TandaH, Berryman DE, Okada S, Tarnowski M, List EO, Kopchick JJ. Generation
and Characterization of Transgenic Mice Expressing the Human CIDE-A cDNA. The 3rd Annual
Diabetes Research Initiative Conference, Athens, OH April 3-4th, 2009.
Kelder B, TandaH, Berryman DE, Okada S, Tarnowski M, List EO, Kopchick JJ. Generation
and Characterization of Transgenic Mice Expressing the Human CIDE-A cDNA. Prolactin and
Growth Hormone Family, Gordon Research Conferance, Ventura, CA, 2008.
Kelder B, Berryman DE, Clark R, Li A, List EO, Kopchick JJ. CIDE-A Gene Expression Is
Decreased In White Adipose Tissue of Growth Hormone Receptor Gene Disrupted Mice and
with High-Fat Feeding of Normal Mice. The Endocrine Society Annual Meeting, Toronto,
Canada, 2007.
List EO, Berryman DE, Palmer AJ, Gosney ES, Kelder B, Kopchick JJ. The Use of Growth
Hormone to Treat Diet Induced Obesity in C57BL/6J Mice. The Endocrine Society Annual
Meeting, Toronto, Canada, 2007.
Kelder B, Boyce K, Nagatomi S, Braughler M, Kopchick JJ. The CIDE-A Gene is Expressed in
Liver of Mice at Older Ages and in Type-II Diabetic Mouse Liver Exhibiting Steatosis. The
Endocrine Society Annual Meeting, San Diego, CA, 2005.
Clark R, Kelder B, Boyce K, Nagatomi S, Braughler M, Kopchick JJ. RGS16 Gene Expression
is Increased in Liver of Hyperinsulinemic and Type II Diabetic Mice. The Endocrine Society
Annual Meeting, San Diego, CA, 2005.
Manhes C, Kayser C, Bertheau P, Kelder B, Kopchick JJ, Kelly PA, Touraine P, Goffin V.
Autocrine PRL leads to Various Mammary Dysfunctions in Transgenic Mice. The Endocrine
Society Annual Meeting, San Diego, CA, 2005.
Kelder B, Boyce K, Matheny G, Kriete A, Dela Cruz S, Braughler M, Kopchick JJ. Rapid and
Dramatic Inhibition of Cyp3a11 and Cyp26 Gene Expression in a Mouse Model of ObesityInduced Type-II Diabetes. The Endocrine Society Annual Meeting, New Orleans, LA, 2004.
Bernichtein S, Kayser C, Dillner K, Kelder B, Kopchick JJ, Isaksson O, Nordstedt G, Martial
JA, Kelly PA, Goffin V. Development of Pure Prolactin Antagonists. The Endocrine Society
Annual Meeting, Philadelphia, PA, 2003.
Li Y, Coschigano, Kelder B, Kopchick JJ. Identification, Isolation and Cloning of Brown
Adipose Tissue cDNAs from Growth Hormone Antagonist Mice. The Endocrine Society Annual
Meeting, Toronto Canada, 2000.
Stevens EC, Kelder B, Kopchick JJ. Expression of the Naturally Occurring Human Growth
Hormone Antagonist hGH R77C in Cultured Mammalian Cells. The Endocrine Society Annual
Meeting, Toronto Canada, 2000.
Hovanec GM, Lewis CJ, Kelder B, Llovera M, Goffin V, Kelly PA, Kopchick JJ. Expression of
Human Prolactin and a Human Prolactiin Antagonist (G129R) in Cultured Mammalian Cells and
Transgenic Animals: Potential Physiological Effects. The Endocrine Society Annual Meeting,
Toronto Canada, 2000.
Andry J, Kelder B, Kopchick JJ. Overexpression of a Histidine-Tagged Growth Hormone
Receptor cDNA in Mice. The Endocrine Society Annual Meeting, Toronto Canada, 2000.
List EO, Kelder B, Kopchick JJ. Growth Hormone Receptor Number for Minimal and Maximal
Growth Hormone Induced STAT-5 Activation. The Endocrine Society Annual Meeting, Toronto
Canada, 2000.
Kelder B, Stone R, Wang X, Weisbrode SE, Darus C, Holle E, Yun JS, Lade DA, Ting J,
Cassiere KL, Begley KB, Long PH, Grant RA, Kopchick JJ. Transgenic Mice Expressing
Human BMP4 cDNA Develop Extensive Osteophytes. The Endocrine Society Annual Meeting,
San Diego, CA, 1999.
Arman A, Billestrup N, Kelder B, Bellush LL, Kopchick JJ. Positive and Negative regulatory
Elements in the Growth Hormone Receptor may be Involved in the Regulation of GH Signaling.
The Endocrine Society Annual Meeting, San Diego, CA, 1999.
List EO, Okada S, Kelder B, Kopchick JJ. Decreased Weight in Mice Containing a Growth
Hormone Receptor (GHR) Ribozy;me (RZ) Gene. The Endocrine Society Annual Meeting, San
Diego, CA, 1999.
List Eo, Okada S, Kelder B, Kopchick JJ. Decreased Biological Function of Mouse Growth
Hormone Receptor (GHR) Mediated by a GHR Ribozyme. The Endocrine Society Annual
Meeting, New Orleans, LA, 1998.
Woodley, FW, Kelder B, Kopchick JJ. Evidence for Redundancy of Exonic Splice Enhancer
Elements Within the Bovine Growth Hormone Gene. The Endocrine Society Annual Meeting,
New Orleans, LA, 1998.
List E, Okada S, Kelder B, Kopchick JJ. Characterization of an anti-mouse growth hormone
receptor ribozyme. The Endocrine Society Annual Meeting, Minneapolis MN, 1997.
Arman A, Kelder B, Okada S, Kopchick JJ. The Effect of Deletion and Repositioning of
Intracellular Regions of the GHR on GH-mediated signal transduction. The Endocrine Society
Annual Meeting, Minneapolis MN, 1997.
Kelder B, Prieto PA, Mukerji P, Erney R, Nardelli C, Kopchick JJ. Production of Secondary
gene products in Mouse Milk by Transgenic Expression of a Human Glycosyltransferase. The
Endocrine Society Annual Meeting, San Francisco, CA, 1996.
Arman A, Kelder B, Xu B, Wang X, Kopchick JJ. Repositioning of a Growth Hormone
Receptor Intracellular Domain Which is Required for STAT5 Activation. The Endocrine Society
Annual Meeting, San Francisco, CA, 1996.
List EO, Zhou Y, Darus CJ, Kelder B, Kopchick, JJ. (June 1996) In vitro cleavage of mouse
growth hormone receptor (GHR) RNA using a GHR specific ribozyme. The Endocrine Society
Annual Meeting, San Francisco, CA, 1996.
Invited Oral Presentations
Kelder B. (May 18, 2009) “Role for CIDE-A in lipid metabolism and liver steatosis” Joint
Meeting on Obesity Research Madrid, SPAIN.
Kelder B. (May 15, 2009) “Role for CIDE-A in lipid metabolism and liver steatosis”. Endocrine
Retreat, Rotterdam, DENMARK.
Kelder B. (Oct 25, 2008) “CIDE-A Gene Expression in Liver and Fat as a Function of Aging,
Diabetes, and GH Receptor Status”. NIH Sponsored Retreat, Port Isobel, VA.
Kelder B.. (Oct 24, 2007) “Role for CIDE-A In Lipid Metabolism and Liver Steatosis:
Discovery of CIDE-A Inhibitors”. Endocrine Retreat, Bushkill, PA.
Kelder B. (April 24, 2006) “Potential role of CIDE-A in liver steatosis”. Endocrinology Retreat,
Edison Biotechnology Institute Athens, OH.
Kelder B (August 30, 2005) “CIDE-A Gene Expression Is Decreased In White Adipose Tissue
of Growth Hormone Receptor Gene Disrupted Mice and with High-Fat Feeding of Normal Mice”
3rd International Congress of Molecular Endocrinology, Paris, FRANCE.
Kelder B. (February 25, 2004) “The CIDE-A Gene is Expressed in Liver of Mice at Older Ages
and in Type-II Diabetic Mouse Liver Exhibiting Steatosis”. Edison Biotechnology Retreat, Ohio
University, Athens, OH.
External Funding
NIH: 1R15DK083729-01A1:
Kelder (PI)
5/01/10 – 4/30/13
“Involvement of CIDE-A in the development of liver steatosis”.
Duration of project: 05/01/2010 to 04/30/2013.
Role: Principle Investigator
Amount Awarded: $ 221,250
NIH/NIDDK-R15 DK075436
Kopchick (PI)
9/1/07-8/31/10
Creation and characterization of GH binding protein gene disrupted mice.
Role: Co-Investigator
$220,500
Sponsored Research Agreement
Kelder (PI)
Elixir Pharmaceuticals, Inc.
Affects of Gene Expression on Aging in Mice
Role: PI
3/01/08 – 2/28/10
Sponsored Research Agreement
Kelder (PI)
Elixir Pharmaceuticals, Inc.
Affects of Gene Expression on Aging in Mice
Role: PI
1/01/07 – 12/31/08
Proprietary & Confidential
Proprietary & Confidential
R01 AG 019899 NIH/NIA
Bartke (PI)
Interaction of Caloric Restriction with Longevity Genes
Role: Co-Investigator
9/1/06-8/31/11
Sponsored Research Agreement
Infectious Disease Resource Institute
“Gene Expression in Transgenic Mice”
Role: PI
Kelder (PI)
07/01/05 – 06/30/07
Sponsored Research Agreement
Elixir Pharmaceuticals, Inc.
“Gene Expression in Transgenic Mice”
Role: PI
Kelder (PI)
Proprietary & Confidential
$ 24,901
01/24/04 – 01/26/06
Proprietary & Confidential
$ 16,639