1
Table S1 Sequence distribution, coverage, and diversity of all WAD sheep rumen samples.
Animal
Experimental
sequence
counts
0.05
OTUs
Good's
coverage
Calculated
maximal
sequences*
Diet I
None
None
None
None
9
9
10
10
2,191
2,355
2,811
2,240
326
301
361
248
93.3%
94.4%
94.3%
95.4%
2,714
2,484
3,468
2,371
45.45
27.03
43.48
19.61
Diet II
None
A. saman
None
A. saman
1
1
2
2
2,396
1,968
2,433
2,237
200
176
236
161
96.6%
96.0%
95.0%
97.0%
1,915
2,037
2,363
2,426
9.71
8.40
8.55
5.29
Diet III
None
G. arborea
None
G. arborea
7
7
8
8
1,860
2,111
1,867
2,048
117
251
324
152
96.8%
95.1%
92.2%
96.6%
2,750
3,198
1,871
1,730
3.51
9.80
40.00
4.03
Diet IV
None
F. sur
None
5
5
6
2,946
2,370
2,733
231
208
268
96.6%
96.5%
95.8%
2,535
2,560
2,800
7.81
8.00
12.35
Group
Tree-based
browse
Inverse
Simpson's
diversity
2
None
3
2,187
278
93.2%
2,092
9.26
B. micrantha
3
2,544
208
97.1%
2,528
24.39
Diet V
None
4
2,456
247
95.6%
2,382
17.86
B. micrantha
4
2,509
279
95.9%
2,093
13.51
*
Number of total sequences needed for the maximum recovery of unique sequences calculated
3
from a second-order polynomial best-fit equation
4
5
Table S2 Fold change in relative sequence abundance in selected ruminal genera due to browse
6
supplementation.
7
P. maximum
Diet I
Genus
N=12
Bacteroides
1.00
Butyrivibrio
1.00
Eubacterium
1.00
Fibrobacter
1.00
Lachnospira
1.00
Megasphaera
1.00
Prevotella
1.00
Ruminococcus
1.00
Selenomonas
1.00
Streptococcus
1.00
Succinivibrio
1.00
*
ND=Not detected
8
A. saman
Diet II
N=2
0.48
0.48
1.17
ND*
ND
ND
0.44
0.81
1.30
0.25
2.37
G. arborea
Diet III
N=2
0.37
1.44
1.07
ND
ND
ND
0.42
0.74
0.37
1.27
1.03
F. sur
Diet IV
N=1
1.07
0.84
1.61
ND
6.01
ND
0.83
0.77
3.14
2.22
19.22
B. micrantha
Diet V
N=2
0.10
1.23
0.76
ND
ND
ND
1.37
0.62
0.22
ND
0.28
9
Supplemental figure legends
10
11
Figure S1 Rarefaction curves for all samples at the 95% OTU level.
12
13
Figure S2 Comparison of phylum-level distribution of 95% OTUs by relative abundance of
14
sequences. The P. maximum-fed group includes both early and late time points for animals 9 and
15
10. P. maximum-fed (Diet I) N=12, A. saman-fed (Diet II) N=2, G. arborea (Diet III) N=2, F.
16
sur-fed (Diet IV) N=1, B. micrantha-fed (Diet V) N=2, all diets N=19.
17
18
Figure S3 Comparison of phylum-level distribution of 95% OTUs by relative abundance of
19
OTUs and sequences for all P. maximum-fed animals. Animals are identified by number, with
20
control animals 9 and 10 having two entries due to being sampled both when all animals were
21
fed P. maximum (“Pre”) and after all other animals (1-8) underwent diet supplementation
22
(“Post”). (A) Phylum-level distribution of 95% OTUs based upon relative abundance of OTUs;
23
(B) phylum-level distribution of 95% OTUs based upon relative abundance of sequences within
24
OTUs.
25
26
Figure S4 Differences in relative sequence abundance for the most abundant genera based on
27
diet. The relative sequence abundance for classifiable genera with >1% abundance in at least one
28
animal is given for each animal on a tree-based browse diet. The mean abundance with standard
29
deviation (black bars) was calculated from the pooled samples taken from all P.maximum-fed
30
animals (n=12, Diet I). Diets: (●) I [control], (□) II, (Δ)III, (×) IV, (◊) V.
31
32
33
34
Figure S1
35
36
37
Figure S2
38
39
Figure S3
40
41
42
Figure S4